公开(公告)号：US09387226B2

公开(公告)日：2016-07-12

申请号：US12516913

申请日：2007-11-29

Applicant: Wonil Oh ,  Yoon-Sun Yang ,  Jong Wook Chang ,  Soo Jin Choi ,  Ju-Yeon Kim

Inventor： Wonil Oh ,  Yoon-Sun Yang ,  Jong Wook Chang ,  Soo Jin Choi ,  Ju-Yeon Kim

IPC: C12N15/00 ,  A61K35/51 ,  C12N5/0797 ,  A61K45/06 ,  C12N5/0789

CPC classification number: A61K35/51 ,  A61K9/0019 ,  A61K35/28 ,  A61K35/50 ,  A61K45/06 ,  A61K2300/00 ,  C12N5/0623 ,  C12N5/0647 ,  C12N2501/01 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2502/03 ,  C12N2502/1358

Abstract: A use of a composition comprising umbilical cord blood-derived mesenchymal stem cells for inducing differentiation and proliferation of neural precursor cells or neural stem cells to neural cells is provided, the composition being effective for the treatment of nerve injury diseases.

Abstract translation: 提供了包含脐带血来源的间充质干细胞用于诱导神经前体细胞或神经干细胞向神经细胞的分化和增殖的组合物的用途，所述组合物对于治疗神经损伤疾病是有效的。

公开(公告)号：US09358255B2

公开(公告)日：2016-06-07

申请号：US14049026

申请日：2013-10-08

Applicant: SpineSmith Partners, L.P.

Inventor： Kevin Dunworth ,  Richard J. Kana ,  Theodore Sand ,  Matthew Murphy

IPC: A61K35/12 ,  A61K35/35 ,  A61K35/14 ,  A61K35/28 ,  C12N5/0775

CPC classification number: A61K35/28 ,  A61K35/14 ,  A61K35/35 ,  C12N5/0663 ,  C12N5/0667 ,  C12N2502/1358 ,  C12N2502/1382 ,  A61K2300/00

Abstract: The invention is directed to a method for isolating stromal cells to form a therapeutic composition; and administering said therapeutic composition to the subject. A further embodiment of the invention is directed to therapeutic compositions comprising isolated stromal cells.

Abstract translation: 本发明涉及分离基质细胞以形成治疗组合物的方法; 并将所述治疗组合物施用于受试者。 本发明的另一个实施方案涉及包含分离的基质细胞的治疗组合物。

公开(公告)号：US20160151418A9

公开(公告)日：2016-06-02

申请号：US13733510

申请日：2013-01-03

Applicant: SANBIO, INC.

Inventor： Mari Dezawa ,  Hajime Sawada ,  Hiroshi Kanno ,  Masahiko Takano

IPC: A61K35/28

CPC classification number: C12N5/0619 ,  A61K9/0085 ,  A61K35/28 ,  A61K35/30 ,  A61K2035/124 ,  C12N5/0618 ,  C12N5/0658 ,  C12N5/0663 ,  C12N2501/42 ,  C12N2502/1358 ,  C12N2506/1353 ,  C12N2510/00

Abstract: There is provided a method of inducing differentiation of bone marrow stromal cells to neural cells or skeletal muscle cells by introduction of a Notch gene. Specifically, the invention provides a method of inducing differentiation of bone marrow stromal cells to neural cells or skeletal muscle cells in vitro, which method comprises introducing a Notch gene and/or a Notch signaling related gene into the cells, wherein the finally obtained differentiated cells are the result of cell division of the bone marrow stromal cells into which the Notch gene and/or Notch signaling related gene have been introduced. The invention also provides a method of inducing further differentiation of the differentiation-induced neural cells to dopaminergic neurons or acetylcholinergic neurons. The invention yet further provides a treatment method for neurodegenerative and skeletal muscle degenerative diseases which employs neural precursor cells, neural cells or skeletal muscle cells produced by the method of the invention.

Abstract translation: 提供了通过引入Notch基因诱导骨髓基质细胞分化为神经细胞或骨骼肌细胞的方法。 具体地说，本发明提供了一种在体外诱导骨髓基质细胞分化为神经细胞或骨骼肌细胞的方法，该方法包括将Notch基因和/或Notch信号传导相关基因引入细胞，其中最终获得的分化细胞 是引入了Notch基因和/或Notch信号传导相关基因的骨髓基质细胞的细胞分裂的结果。 本发明还提供了诱导分化诱导的神经细胞进一步分化为多巴胺能神经元或乙酰胆碱能神经元的方法。 本发明还进一步提供了一种神经变性和骨骼肌退行性疾病的治疗方法，其采用通过本发明的方法生产的神经前体细胞，神经细胞或骨骼肌细胞。

公开(公告)号：US20160040120A1

公开(公告)日：2016-02-11

申请号：US14821994

申请日：2015-08-10

Applicant: Karlsruher Institut für Technologie ,  Ruprecht-Karls-Universität Heidelberg

Inventor： Eric Gottwald ,  Stefan Giselbrecht ,  Patrick Wuchter ,  Rainer Saffrich

IPC: C12N5/00

CPC classification number: C12N5/0062 ,  C12N5/0647 ,  C12N2502/1171 ,  C12N2502/13 ,  C12N2502/1305 ,  C12N2502/1311 ,  C12N2502/1317 ,  C12N2502/1323 ,  C12N2502/1329 ,  C12N2502/1335 ,  C12N2502/1341 ,  C12N2502/1347 ,  C12N2502/1352 ,  C12N2502/1358 ,  C12N2502/1364 ,  C12N2502/137 ,  C12N2502/1376 ,  C12N2502/1382 ,  C12N2502/1388 ,  C12N2502/1394 ,  C12N2513/00 ,  C12N2531/00

Abstract: The present invention relates to a three-dimensional (3D) model of a hematopoietic stem and progenitor cell (HSPC) niche, that comprises the coculture of human HSPC and human mesenchymal stromal cells in a defined 3D environment and thereby procures vital stem cell functions.

Abstract translation: 本发明涉及造血干细胞和祖细胞（HSPC）利基的三维（3D）模型，其包括在定义的3D环境中人HSPC和人间充质基质细胞的共培养，从而获得重要的干细胞功能。

公开(公告)号：US20150282885A1

公开(公告)日：2015-10-08

申请号：US14678392

申请日：2015-04-03

Applicant: ORGANOVO, INC.

Inventor： Shelby Marie KING ,  Deborah Lynn Greene NGUYEN ,  Vivian A. GORGEN ,  Benjamin R. SHEPHERD ,  Sharon C. PRESNELL

IPC: A61B19/00 ,  C12N5/09

CPC classification number: G01N33/5011 ,  B33Y10/00 ,  B33Y80/00 ,  C12N5/0656 ,  C12N5/0693 ,  C12N5/0697 ,  C12N2502/13 ,  C12N2502/1305 ,  C12N2502/1323 ,  C12N2502/1358 ,  C12N2502/28 ,  C12N2513/00 ,  C12N2533/74 ,  G01N33/5082

Abstract: Described are three-dimensional, engineered, biological breast tissues, adipose tissues, and tumor models, including breast cancer models.

Abstract translation: 描述了三维，工程化，生物乳腺组织，脂肪组织和肿瘤模型，包括乳腺癌模型。

公开(公告)号：US20150064273A1

公开(公告)日：2015-03-05

申请号：US14378221

申请日：2013-02-13

Applicant: Gamida-Cell Ltd.

Inventor： Tony Peled ,  Yair Steinhardt

IPC: C12N5/0775 ,  A61K35/28

CPC classification number: C12N5/0667 ,  A61K35/28 ,  A61K2035/124 ,  C12N5/0663 ,  C12N2500/38 ,  C12N2501/119 ,  C12N2502/1358 ,  C12N2502/1382

Abstract: Mesenchymal stem cell conditioned medium and methods for preparing the conditioned medium are provided. The methods comprise culturing MSCs in a medium comprising nicotinamide or nicotinamide and fibroblast growth factor 4 (FGF4) and collecting the conditioned medium. Compositions comprising the mesenchymal stem cell conditioned medium and uses thereof are also provided.

Abstract translation: 提供间充质干细胞条件培养基和制备条件培养基的方法。 所述方法包括在包含烟酰胺或烟酰胺和成纤维细胞生长因子4（FGF4）的培养基中培养MSC并收集条件培养基。 还提供了包含间充质干细胞条件培养基的组合物及其用途。

公开(公告)号：US20150037298A1

公开(公告)日：2015-02-05

申请号：US14380155

申请日：2013-02-21

Applicant: BrainStem Biotec Ltd. ,  Henry Ford Health System

Inventor： Chaya Brodie ,  Shimon Slavin

IPC: A61K35/30 ,  C12Q1/68 ,  C12N5/079

CPC classification number: A61K35/30 ,  A61K35/28 ,  A61K35/50 ,  A61K35/51 ,  C12N5/0622 ,  C12N15/113 ,  C12N2310/141 ,  C12N2320/11 ,  C12N2330/10 ,  C12N2501/01 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/135 ,  C12N2501/195 ,  C12N2501/41 ,  C12N2501/65 ,  C12N2502/13 ,  C12N2502/1305 ,  C12N2502/1311 ,  C12N2502/1317 ,  C12N2502/1323 ,  C12N2502/1329 ,  C12N2502/1335 ,  C12N2502/1341 ,  C12N2502/1347 ,  C12N2502/1352 ,  C12N2502/1358 ,  C12N2502/1364 ,  C12N2502/137 ,  C12N2502/1376 ,  C12N2502/1382 ,  C12N2502/1388 ,  C12N2502/1394 ,  C12N2506/025 ,  C12N2506/1353 ,  C12N2506/1369 ,  C12N2506/1384 ,  C12N2506/1392 ,  C12N2510/00 ,  C12Q1/6876 ,  C12Q2600/178

Abstract: A method of generating a population of cells useful for treating a nerve disease or disorder in a subject, the method comprising up-regulating a level of at least one exogenous miRNA in mesenchymal stem cells (MSCs) and/or down-regulating a level of at least one miRNA using a polynucleotide agent that hybridizes to the miRNA, thereby generating the population of cells useful for treating the nerve disease or disorder. Isolated populations of cells with an astrocytic phenotype generated thereby and uses thereof are also provided.

Abstract translation: 一种产生可用于治疗受试者神经疾病或病症的细胞群的方法，所述方法包括上调间充质干细胞（MSC）中至少一种外源miRNA的水平和/或下调 使用与miRNA杂交的多核苷酸试剂的至少一种miRNA，从而产生可用于治疗神经疾病或病症的细胞群。 还提供了由此产生的具有星形细胞表型的细胞的分离群体及其用途。

公开(公告)号：US20140377832A1

公开(公告)日：2014-12-25

申请号：US14371640

申请日：2013-01-14

Applicant: YEDA RESEARCH AND DEVELOPMENT CO. LTD.

Inventor： Dov Zipori ,  Ofer Shoshani

IPC: C12N5/074 ,  C12N13/00

CPC classification number: C12N5/0696 ,  C12N5/0663 ,  C12N13/00 ,  C12N2500/02 ,  C12N2502/13 ,  C12N2502/1305 ,  C12N2502/1311 ,  C12N2502/1317 ,  C12N2502/1323 ,  C12N2502/1329 ,  C12N2502/1335 ,  C12N2502/1341 ,  C12N2502/1347 ,  C12N2502/1352 ,  C12N2502/1358 ,  C12N2502/1364 ,  C12N2502/137 ,  C12N2502/1376 ,  C12N2502/1382 ,  C12N2502/1388 ,  C12N2502/1394 ,  C12N2506/1353 ,  C12N2510/00

Abstract: The invention relates to induction of reprogramming of somatic cells, by methods which require mild growth conditions. Disclosed are methods of inducing dedifferentiation of mesenchymal stromal cell (MSC), by seeding or incubating mesenchymal stromal cells (MSCs) at low density, and without introduction or expression of exogenous genes in the cells.

Abstract translation: 本发明涉及通过需要温和生长条件的方法诱导体细胞重编程。 公开了通过以低密度接种或培养间充质基质细胞（MSC）诱导间充质基质细胞（MSC）去分化的方法，并且不在细胞中引入或表达外源基因。

公开(公告)号：US20140322135A1

公开(公告)日：2014-10-30

申请号：US14266480

申请日：2014-04-30

Applicant: Valerie Roobrouck ,  Michel Delforge ,  Catherine M. Verfaillie

Inventor： Valerie Roobrouck ,  Michel Delforge ,  Catherine M. Verfaillie

IPC: A61K35/54 ,  G01N33/50 ,  A61K49/00

CPC classification number: A61K35/545 ,  A61K49/0004 ,  C12N5/0607 ,  C12N2502/13 ,  C12N2502/1305 ,  C12N2502/1311 ,  C12N2502/1317 ,  C12N2502/1323 ,  C12N2502/1329 ,  C12N2502/1335 ,  C12N2502/1341 ,  C12N2502/1347 ,  C12N2502/1352 ,  C12N2502/1358 ,  C12N2502/1364 ,  C12N2502/137 ,  C12N2502/1376 ,  C12N2502/1382 ,  C12N2502/1388 ,  C12N2502/1394 ,  G01N33/5044

Abstract: The invention provides methods for treating myelodysplastic syndrome (MDS). The invention is generally directed to reducing certain overt symptoms and disease-causing biological events in MDS by administering certain cells to a subject having MDS. The invention is also directed to drug discovery methods to screen for agents that modulate the ability of the cells to affect these events. The invention is also directed to cell banks that can be used to provide cells for administration to a subject, the banks comprising cells having desired potency for affecting these events.

Abstract translation: 本发明提供治疗骨髓增生异常综合征（MDS）的方法。 本发明一般涉及通过向具有MDS的受试者施用某些细胞来减少MDS中的某些明显症状和引起疾病的生物学事件。 本发明还涉及用于筛选调节细胞影响这些事件的能力的药剂的药物发现方法。 本发明还涉及可用于向受试者提供细胞的细胞库，所述细胞库包含具有影响这些事件的期望效力的细胞。

公开(公告)号：US08828375B2

公开(公告)日：2014-09-09

申请号：US13061693

申请日：2009-09-03

Applicant: Silviu Itescu ,  Michael David Schuster

Inventor： Silviu Itescu ,  Michael David Schuster

IPC: A61K38/20 ,  A61K35/14 ,  A61K38/19 ,  A61P35/02 ,  A61P35/00 ,  A61P7/00 ,  A61P19/02 ,  A61P31/12 ,  A61P31/06 ,  A61P7/06 ,  C12N5/0789 ,  A61K35/12

CPC classification number: A61K35/28 ,  A61K45/06 ,  A61K2035/124 ,  C12N5/0647 ,  C12N2501/125 ,  C12N2501/145 ,  C12N2501/22 ,  C12N2501/26 ,  C12N2502/1352 ,  C12N2502/1358

Abstract: The present invention relates to a method of transplanting haematopoietic precursor cells into a subject in need thereof which involves culturing the haematopoietic precursor cells in the presence of a population of cells enriched for STRO-1bright cells. The method of the present invention is useful in the treatment of haematological disorders.

Abstract translation: 本发明涉及将造血前体细胞移植到有需要的受试者中的方法，其涉及在富含STRO-1Bight细胞的细胞群的存在下培养造血前体细胞。 本发明的方法可用于治疗血液病。