利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

12452 条记录 (耗时 0.041 秒)

    Apparatus and method for increasing insect resistance in transgenic plants
    41.
    发明申请
    Apparatus and method for increasing insect resistance in transgenic plants 审中-公开
    用于增加转基因植物抗虫性的装置和方法

    公开(公告)号：US20040259080A1

    公开(公告)日：2004-12-23

    申请号：US09956326

    申请日：2001-09-18

    发明人： Gary W. Felton ,  Yinong Yang ,  Qin Wang

    IPC分类号： C12Q001/68 ,  C07H021/04 ,  C12N009/02

    CPC分类号： A01N37/46 ,  A01N63/02 ,  C12N9/0006 ,  C12Q1/26

    摘要： The present invention discloses a polynucleotide sequence for an insect salivary glucose oxidase enzyme and the amino acid sequence of the enzyme itself. It also provides recombinant polynucleotide vector systems designed to express the enzyme in a variety of host organisms. The invention also discloses a method for creating transgenic plants having increased resistance to insect predation resulting from the expression of the foreign glucose oxidase protein. The presence of the insect glucose oxidase enzyme triggers a plant's defensive mechanisms and results in increased resistance to insects.

    摘要翻译： 本发明公开了一种昆虫唾液葡萄糖氧化酶的多核苷酸序列和酶本身的氨基酸序列。 它还提供设计用于在多种宿主生物体中表达酶的重组多核苷酸载体系统。 本发明还公开了一种产生转基因植物的方法，所述转基因植物具有增加的抗外源葡萄糖氧化酶蛋白质表达引起的昆虫捕食的能力。 昆虫葡萄糖氧化酶的存在触发植物的防御机制，并导致对昆虫的抗性增加。

    Blueberry red ringspot virus, sequences, promoters, and uses thereof
    44.
    发明申请
    Blueberry red ringspot virus, sequences, promoters, and uses thereof 失效
    蓝莓红环斑病毒，序列，启动子及其用途

    公开(公告)号：US20040255348A1

    公开(公告)日：2004-12-16

    申请号：US10793454

    申请日：2004-03-04

    发明人： Richard F. Allison ,  Jerri Gillett ,  Christy Mecey

    IPC分类号： C12N015/82 ,  C12Q001/68 ,  C12N015/87 ,  C12N005/10 ,  C07H021/04

    CPC分类号： C07K14/005 ,  C12N15/8227 ,  C12N2710/00022

    摘要： A nucleic acid sequence of the blueberry red ringspot virus is disclosed. Also disclosed are putative promoter regions of the sequence and promoter regions capable of directing transgene expression in plants, including tissue-specific expression. Also disclosed are expression vectors, transformed plant cells and plants containing a blueberry red ringspot virus promoter and an encoded product for expression. Methods for diagnosis of blueberry red ringspot virus infection are also provided.

    摘要翻译： 公开了蓝莓红环斑病毒的核酸序列。 还公开了能够指导植物中转基因表达的序列和启动子区域的推定启动子区域，包括组织特异性表达。 还公开了含有蓝莓红环斑病毒启动子和用于表达的编码产物的表达载体，转化的植物细胞和植物。 还提供了蓝莓红环斑病毒感染的诊断方法。

    Circular site-directed mutagenesis
    45.
    发明申请
    Circular site-directed mutagenesis 有权

    公开(公告)号：US20040253729A1

    公开(公告)日：2004-12-16

    申请号：US10811062

    申请日：2004-03-25

    申请人： Stratagene and Children's Medical Center Corporation

    发明人： John C. Bauer ,  Dowain A. Wright ,  Jeffrey Carl Braman ,  Raif S. Geha

    IPC分类号： C12Q001/68 ,  C12P019/34 ,  C12N015/87 ,  C12N015/74

    CPC分类号： C12N15/102 ,  C12Q1/6806 ,  C12Q1/6827 ,  C12Q1/6853 ,  C12Q2600/156 ,  Y10S435/81 ,  C12Q2525/307 ,  C12Q2521/319 ,  C12Q2525/185 ,  C12Q2521/331 ,  C12Q2521/101 ,  C12Q2531/125

    摘要： The invention provides improved methods of introducing site-directed mutations into circular DNA molecules of interest by means of mutagenic primer pairs. The mutagenic primer pairs are also selected so as to be either completely complementary or partially complementary to each other, wherein the mutation site (or sites) is located within the region of complementarity. A mutagenic primer pair is annealed to opposite strands of a circular DNA molecule containing the DNA sequence to be mutagenized. After annealing, first and second mutagenized DNA strands, each incorporating a member of the mutagenic oligonucleotide primer pair is synthesized by a linear cyclic amplification reaction. After the linear cyclic amplification mediated synthesis step is completed, the reaction mixture is treated with a selection enzyme that digests the parental template strands. After the digesting step, a double-stranded circular DNA intermediate is formed. The double-stranded circular DNA intermediates is transformed in suitable competent host cells and closed circular double-stranded DNA corresponding to the parental template molecules, but containing the desired mutation or mutations of interest, may be conveniently recovered from the transformed cells. The invention also provide kits for site-directed mutagenesis in accordance with methods of the present invention.

    COMPOSITIONS AND METHODS FOR MAKING MUTATIONS IN CELL LINES AND ANIMALS
    46.
    发明申请
    COMPOSITIONS AND METHODS FOR MAKING MUTATIONS IN CELL LINES AND ANIMALS 有权

    公开(公告)号：US20040253727A1

    公开(公告)日：2004-12-16

    申请号：US10342761

    申请日：2003-01-15

    申请人： Athersys, Inc.

    发明人： John Joseph Harrington ,  Paul David Jackson ,  Li Jiang

    IPC分类号： C12Q001/68 ,  C12N015/85

    CPC分类号： C12N15/102 ,  C12N15/01 ,  C12Q1/6897

    摘要： Abstract of DisclosureThe present invention is directed generally to reduction or inactivation of gene function or gene expression in cells in vitro and in multiorganisms. The invention encompasses methods for mutating cells using a combination of mutagens, particularly wherein at least one mutagen is an insertional mutagen, to achieve homozygous gene mutation or mutation of multiple genes required cumulatively to achieve a phenotype to create knock-outs, knock-downs, and other modifications in the same cell. The invention is also directed to cells (and libraries thereof) and organisms created by the methods of the invention, including those in which at least one of the genes created by insertional mutagenesis is tagged by means of the insertion sequences thereby allowing identification of the mutated gene(s). The invention is also directed to libraries of mutated cells and their uses. The invention is also directed to methods of identifying mutations with methods of the invention, in cells (and libraries thereof) and organisms, by means of the insertional tag.

    Methods and kits for detecting protein kinases
    47.
    发明申请
    Methods and kits for detecting protein kinases 审中-公开
    用于检测蛋白激酶的方法和试剂盒

    公开(公告)号：US20040253658A1

    公开(公告)日：2004-12-16

    申请号：US10892285

    申请日：2004-07-16

    申请人： Lumitech (UK) Limited

    发明人： Sharon Patricia Mary Crouch ,  Kevin John Slater

    IPC分类号： C12Q001/68 ,  C12Q001/48

    CPC分类号： C12Q1/485 ,  G01N2500/04

    摘要： Methods and kits for detecting kinase activity A method for measuring protein kinase activity comprising: (a) providing a first solution comprising ATP and a protein kinase to be tested, and a second solution comprising ATP in the absence of said kinase to be tested; (b) adding a substrate capable of being phosphorylated by the protein kinase to be tested to the first and second solutions of step (a); (c) measuring the concentration of ATP and/or ADP, or the rate of change thereof with respect to time, in each of the reaction mixtures formed in step (b) using a bioluminescence reaction; and (d) using the information about the concentration of ATP and/or ADP to determine the activity of the protein kinase to be tested.

    摘要翻译： 用于检测激酶活性的方法和试剂盒用于测量蛋白激酶活性的方法，包括：（a）提供包含ATP和待测试的蛋白激酶的第一溶液，以及在不存在所述待测试激酶的情况下包含ATP的第二溶液; （b）将待测试的蛋白激酶磷酸化的底物加入到步骤（a）的第一和第二溶液中; （c）使用生物发光反应测量在步骤（b）中形成的每种反应混合物中ATP和/或ADP的浓度或其相对于时间的变化速率; 和（d）使用关于ATP和/或ADP的浓度的信息来确定待测试的蛋白激酶的活性。

    Protein quantitation with cell imaging densitometry
    48.
    发明申请
    Protein quantitation with cell imaging densitometry 审中-公开
    蛋白质定量与细胞成像光密度测定

    公开(公告)号：US20040253649A1

    公开(公告)日：2004-12-16

    申请号：US10806570

    申请日：2004-03-22

    发明人： Steven J. Smith

    IPC分类号： C12Q001/68 ,  G01N033/53 ,  G01N033/567 ,  G01N033/574

    CPC分类号： G01N33/57496 ,  G01N1/30 ,  G01N15/1475 ,  G01N33/574 ,  G01N33/57484 ,  G01N2015/1006 ,  G01N2800/52

    摘要： A method for quantitating cellular proteins in tissue, by means of a cell imaging densitometer in conjunction with immunohistological staining and a reference standard, is provided. Unlike prior art methods, which provide ordinal measures of relative amounts of protein among different cells, the method enables the quantitation of antigenic proteins in terms of absolute mass of protein/tumor or protein/patient, molecules of protein per cell, and volume or fraction of a tissue sample expressing the protein of interest. The method is useful for research purposes in the study of protein expression, and is shown to improve the accuracy of clinical histopathological analysis of tumor tissue sections for diagnosis and prognosis. The method is expected to be useful for prescribing in situ treatment dosages. The demonstrated resulting improvement in the correlation between tissue levels and blood levels of tumor-associated proteins should facilitate minimally-invasive monitoring of cancer progression and therapeutic response.

    摘要翻译： 提供了通过细胞成像密度计结合免疫组织染色和参考标准定量组织中的细胞蛋白的方法。 不同于提供不同细胞中蛋白质相对量的顺序测量的现有技术方法，该方法能够根据蛋白质/肿瘤或蛋白质/患者的绝对质量，每个细胞的蛋白质分子和体积或分数来定量抗原蛋白质 的表达目的蛋白质的组织样品。 该方法在研究蛋白质表达的研究中是有用的，并且显示提高了肿瘤组织切片临床组织病理学分析的准确性，用于诊断和预后。 该方法预期对于处方原位治疗剂量是有用的。 所证实的组织水平与肿瘤相关蛋白的血液水平之间的相关性的改善可促进对癌症进展和治疗反应的微创监测。