公开(公告)号：US06911305B2

公开(公告)日：2005-06-28

申请号：US10813284

申请日：2004-03-30

Applicant: Derek W. K. Levison ,  Uwe Moller ,  Stuart Levison

Inventor： Derek W. K. Levison ,  Uwe Moller ,  Stuart Levison

IPC: C09K11/07 ,  C12M1/34 ,  C12M3/00 ,  C12Q20060101 ,  C12Q1/00 ,  C12Q1/04 ,  C12Q1/08 ,  C12Q1/28 ,  C12Q1/42 ,  C12Q1/68 ,  G01N33/53 ,  G01N33/543 ,  G01N33/558 ,  G01N33/569 ,  G03C5/04

CPC classification number: C09K11/07 ,  C12Q1/04 ,  C12Q1/6816 ,  G01N33/543 ,  G01N33/558 ,  Y10S435/968 ,  Y10S435/975

Abstract: The invention provides chemiluminescent assays that incorporate a film including at least one chemiluminescent precursor immobilized therewith which produces a triggerable chemiluminescent compound, the film being free of compounds which generate singlet oxygen and being adapted for use with a sensitizer-labeled agent or agent probative of the analyte.

Abstract translation: 本发明提供化学发光测定法，其包括包含至少一种固定化合物的化学发光前体的膜，其产生可触发的化学发光化合物，该膜不含产生单线态氧的化合物，并适用于敏化剂标记的试剂或试剂 分析物。

公开(公告)号：US6126899A

公开(公告)日：2000-10-03

申请号：US831983

申请日：1997-04-02

Applicant: Timothy W. Woudenberg ,  Michael Albin ,  Reid B. Kowallis ,  Yefim Raysberg ,  Robert P. Ragusa ,  Emily S. Winn-Deen

Inventor： Timothy W. Woudenberg ,  Michael Albin ,  Reid B. Kowallis ,  Yefim Raysberg ,  Robert P. Ragusa ,  Emily S. Winn-Deen

IPC: C12N15/09 ,  B01L3/00 ,  B01L7/00 ,  C12M1/00 ,  C12Q1/08 ,  C12Q1/68 ,  G01N33/53 ,  G01N33/543 ,  G01N35/00 ,  G01N1/14 ,  G01N15/06 ,  G01N21/00 ,  G01N21/64

CPC classification number: G01N33/5304 ,  B01L3/5027 ,  B01L3/50273 ,  C12Q1/08 ,  B01L2200/027 ,  B01L2200/0642 ,  B01L2200/141 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/1827 ,  B01L2400/049 ,  B01L7/52 ,  G01N2035/00148 ,  Y10S435/973 ,  Y10S436/807

Abstract: The invention is directed to a method and device for simultaneously testing a sample for the presence, absence, and/or amounts of one or more a plurality of selected analytes. The invention includes, in one aspect, a device for detecting or quantitating a plurality of different analytes in a liquid sample. The device includes a substrate which defines a sample-distribution network having (i) a sample inlet, (ii) one or more detection chambers, and (iii) channel means providing a dead-end fluid connection between each of the chambers and the inlet. Each chamber may include an analyte-specific reagent effective to react with a selected analyte that may be present in the sample, and detection means for detecting the signal. Also disclosed are methods utilizing the device.

Abstract translation: 本发明涉及一种用于同时测试样品的一种或多种选择的分析物的存在，不存在和/或量的方法和装置。 本发明在一个方面包括用于检测或定量液体样品中的多种不同分析物的装置。 该装置包括限定样品分配网络的基板，其具有（i）样品入口，（ii）一个或多个检测室，以及（iii）通道装置，其在每个室之间提供死端流体连接，并且入口 。 每个室可以包括有效地与可能存在于样品中的选定分析物反应的分析物特异性试剂和用于检测信号的检测装置。 还公开了利用该装置的方法。

公开(公告)号：US6008009A

公开(公告)日：1999-12-28

申请号：US174380

申请日：1998-10-15

Applicant: Mark S. F. Clarke ,  Daniel L. Feeback

Inventor： Mark S. F. Clarke ,  Daniel L. Feeback

IPC: G01N1/30 ,  G01N1/31 ,  G01N35/00 ,  C12Q1/00 ,  C12Q1/08

CPC classification number: G01N1/30 ,  G01N1/31 ,  G01N2035/00336 ,  Y10S435/96

Abstract: A method of staining preselected, mounted specimens of either biological or nonbiological material enclosed within a staining chamber where the liquid staining reagents are applied and removed from the staining chamber using hypergravity as the propelling force. In the preferred embodiment, a spacecraft-operated centrifuge and method of diagnosing biological specimens while in orbit, characterized by hermetically sealing a shell assembly. The assembly contains slide stain apparatus with computer control therefor, the operative effect of which is to overcome microgravity, for example on board an International Space Station.

Abstract translation: 将预先选定的，安装在染色室内的生物或非生物材料的样品的染色方法，其中使用超重力作为推进力施加液体染色试剂并从染色室除去。 在优选实施例中，航天器操作的离心机和在轨道上诊断生物样本的方法，其特征在于密封壳体组件。 该组件包含具有计算机控制的滑动污渍装置，其操作效果是克服微重力，例如在国际空间站上。

公开(公告)号：US5801151A

公开(公告)日：1998-09-01

申请号：US761470

申请日：1996-12-06

Applicant: Jack Van't Hof

Inventor： Jack Van't Hof

IPC: G01N1/30 ,  G01N33/36 ,  C12Q1/08 ,  A01N43/04

CPC classification number: G01N1/30 ,  G01N33/362

Abstract: The present invention is a method for rendering cotton fiber cells that are post-anthesis and pre-harvest available for analysis of their physical properties. The method includes the steps of hydrolyzing cotton fiber cells and separating cotton fiber cells from cotton ovules thereby rendering the cells available for analysis. The analysis of the fiber cells is through any suitable means, e.g., visual inspection. Visual inspection of the cells can be accomplished by placing the cells under an instrument for detection, such as microscope or other means.

Abstract translation: 本发明是一种用于使棉花纤维细胞在开花期和预收获后可用于分析其物理性质的方法。 该方法包括水解棉纤维细胞并从棉花胚珠分离棉花纤维细胞的步骤，从而使细胞可用于分析。 纤维细胞的分析是通过任何合适的手段，例如目视检查。 可以通过将细胞放置在用于检测的仪器（例如显微镜或其它方法）下来实现细胞的目视检查。

公开(公告)号：US5691164A

公开(公告)日：1997-11-25

申请号：US563192

申请日：1995-11-27

Applicant: Eric N. Speiser

Inventor： Eric N. Speiser

IPC: A01N1/00 ,  A01N3/00 ,  C12Q1/08

CPC classification number: A01N1/00 ,  A01N3/00

Abstract: A tissue fixative comprising a solvent including one or more alkanols, one or more diols and/or triols and a catalyst, and a solute including an osmotically active substance and a mordant. A preferred composition comprises 14 ml ethanol, 14 ml ethandiol, 2.43 ml of a 37% solution of methanal, 0.375 g NaCl, 0.0375 g ZaCl.sub.2 and 69.57 mls water.

Abstract translation: 一种组织固定剂，其包含溶剂，其包含一种或多种链烷醇，一种或多种二醇和/或三醇和催化剂，以及包含渗透活性物质和媒染剂的溶质。 优选的组合物包含14ml乙醇，14ml乙二醇，2.43ml 37％甲醛溶液，0.375g NaCl，0.0375g ZaCl 2和69.57ml水。

公开(公告)号：US5650334A

公开(公告)日：1997-07-22

申请号：US521860

申请日：1995-08-31

Applicant: Robert F. Zuk ,  Sae Hyun Choo

Inventor： Robert F. Zuk ,  Sae Hyun Choo

IPC: C07K17/10 ,  C09B23/00 ,  C09K11/06 ,  C12N11/10 ,  C12Q1/08 ,  C12Q1/68 ,  G01N33/533 ,  G01N33/548 ,  G01N37/00 ,  G01N33/544 ,  G01N33/53 ,  G01N33/58 ,  G01N33/60

CPC classification number: G01N33/533 ,  Y10S435/96 ,  Y10S435/968 ,  Y10S436/80

Abstract: A fluorescent labelling composition comprises a linear polysaccharide backbone molecule having a plurality of target-binding molecules, such as antibodies or nucleic acids, attached at spaced-apart intervals thereon. Each of the target-binding molecules, in turn, includes a multiplicity of fluorescent dye molecules bound thereto. In this way, fluorescent signal introduced to a single target-site on a solid phase surface may be increased without loss of binding activity.

Abstract translation: 荧光标记组合物包含线性多糖主链分子，其具有以间隔开间隔连接的多个靶结合分子，例如抗体或核酸。 靶结合分子中的每一个又包含与其结合的多个荧光染料分子。 以这种方式，可以增加引入固相表面上的单个靶位点的荧光信号，而不损失结合活性。

公开(公告)号：US5624815A

公开(公告)日：1997-04-29

申请号：US302905

申请日：1994-12-05

Applicant: Peter L. Grant ,  Nicholas P. M. Foote ,  Michael Noble ,  Christopher T. Evans

Inventor： Peter L. Grant ,  Nicholas P. M. Foote ,  Michael Noble ,  Christopher T. Evans

IPC: B01L3/00 ,  C12Q1/04 ,  C12Q1/06 ,  C12Q1/68 ,  G01N33/53 ,  C12Q1/24 ,  C12M1/12 ,  C12M1/34 ,  C12Q1/08

CPC classification number: B01L3/50853 ,  B01L3/5025 ,  B01L3/50255 ,  C12Q1/04 ,  C12Q1/06 ,  G01N33/5302

Abstract: A method for analyzing solid material in a liquid sample comprises the steps of: distributing the sample equally by passage through a number of discrete wells adapted to retain the solid material, the concentration of solid material being such that it is absent in at least one well; and analyzing the wells for the presence of retained solid material. A device adapted for use in a method for analyzing solid material in a liquid sample comprises the combination of: a container for the sample; a unit comprising a number of discrete wells adapted to retain the solid material and allow the passage of liquid under the application of reduced pressure; structure for drawing liquid from the container and through the wells under reduced pressure; and a manifold or other element that provides uniform distribution of the sample passing from the container into the wells.

Abstract translation: PCT No.PCT / GB93 / 00577 Sec。 371日期1994年12月5日第 102（e）日期1994年12月5日PCT 1993年3月22日PCT公布。 公开号WO93 / 19199 日期1993年9月30日一种用于分析液体样品中的固体材料的方法包括以下步骤：通过穿过适于保持固体材料的多个离散孔平均分配样品，固体材料的浓度使得其不存在 在至少一口井中; 并分析井中是否存在保留的固体材料。 适用于分析液体样品中固体物质的方法的装置包括以下组合：用于样品的容器; 包括适于保持固体材料并允许在减压下使液体通过的多个不连续孔的单元; 用于从容器中抽取液体并在减压下通过井的结构; 以及提供从容器进入孔中的样品均匀分布的歧管或其它元件。

公开(公告)号：US5508175A

公开(公告)日：1996-04-16

申请号：US252113

申请日：1994-06-01

Applicant: Malcolm Slifkin

Inventor： Malcolm Slifkin

IPC: C12Q1/04 ,  G01N1/30 ,  C12Q1/08 ,  C12Q1/02 ,  C12Q3/00

CPC classification number: C12Q1/04 ,  G01N1/30

Abstract: A fixative-stain system, which gives superior preservation of nuclear detail, is free from toxic mercury compounds, and which is simple and easy to use, includes a zinc salt and a cobalt salt, in combination, as a fixative, and at least one of Chlorazol Black E, Fast Green FCF and May-Grunwald stains, and preferably the three in admixture, as a staining composition. The fixative may also be used alone. The present fixative-stain system is suitable for fixing and staining all types of parasites such as enteric and other parasites which infect animals and humans.

Abstract translation: 具有优异的核细节保存性的固色染色系统不含有毒的汞化合物，其简单易用，包括锌盐和钴盐，作为固定剂，至少一种 的氯唑黑E，Fast Green FCF和May-Grunwald污渍，优选三种混合物作为染色组合物。 固定剂也可以单独使用。 本发明的固定染色系统适用于固定和染色所有类型的寄生虫，如感染动物和人类的肠道和其他寄生虫。

公开(公告)号：US4503149A

公开(公告)日：1985-03-05

申请号：US501480

申请日：1983-06-06

Applicant: Phil A. Boyd

Inventor： Phil A. Boyd

IPC: C12Q1/06 ,  C12Q1/08 ,  G01N21/33

CPC classification number: C12Q1/06 ,  Y10S435/808 ,  Y10T436/143333 ,  Y10T436/25375

Abstract: A process for detecting the population count of microorganisms in an aqueous medium by means of ultraviolet spectrophotometry. The specimen is first filtered and the resulting filtrate then centrifuged to concentrate the microbial cells in a pellet layer. The microbial cells are then redispersed in an aqueous solution having a volume less than the volume of the original specimen and the absorption of ultraviolet light by the redispersed solution at wavelengths within the range of 260-280 nanometers is measured. The concentrated microbial cells may be lysed in order to release nucleic acid and protein components from the cellular wall structure.

Abstract translation: 一种通过紫外分光光度法检测水介质中微生物群体数量的方法。 首先过滤样品，然后离心所得滤液，将微生物细胞浓缩成颗粒层。 然后将微生物细胞再分散在体积小于原始样品的体积的水溶液中，并测量通过再分散溶液在260-280纳米范围内的波长的紫外光吸收。 可以将浓缩的微生物细胞裂解以从细胞壁结构释放核酸和蛋白质组分。

公开(公告)号：US4247634A

公开(公告)日：1981-01-27

申请号：US41223

申请日：1979-05-21

Applicant: Mohamed Abdou

Inventor： Mohamed Abdou

IPC: C12M1/16 ,  C12M1/20 ,  C12Q1/08

CPC classification number: C12M23/38 ,  C12M23/12

Abstract: A culture cup for sampling and microbial-count determination, comprising a body and a tightly closing removable lid, the bottom of the body being provided with depressions for accomodation of at least one culture medium. The lid on its underside may be provided with a recess for accommodation of a condensate absorbent, and the body of the cup with a shoulder to serve as a seat for the bottom of the lid. The lid and body are threaded for engagement. By placing a culture medium in the depression, a biological specimen such as urine or blood can be added in just sufficient quantity to wet the culture so pouring and handling are reduced. Culture and analysis can then be undertaken in the usual manner using the cup body as the carrier of the culture medium.

Abstract translation: 一种用于取样和微生物计数测定的培养杯，包括主体和紧密封闭的可移除盖，所述身体的底部设置有用于容纳至少一种培养基的凹陷。 其下侧的盖可以设置有用于容纳冷凝物吸收剂的凹部和具有肩部的杯体，以用作盖的底部的座。 盖子和主体被螺纹接合。 通过将培养基放置在抑郁症中，可以仅添加足够量的生物样品如尿液或血液来润湿培养物，从而减少浇注和处理。 然后可以以通常的方式使用杯体作为培养基的载体来进行培养和分析。