公开(公告)号：US20190225656A1

公开(公告)日：2019-07-25

申请号：US16375140

申请日：2019-04-04

Applicant: Genia Technologies, Inc.

Inventor： Michael Dorwart ,  Daniel Korenblum

IPC: C07K14/31 ,  C12Q1/6869

Abstract: Described herein are variants of alpha-hemolysin having at least one mutation selected from T12R, T12K, N17R, N17K or combinations of T12 and N17 mutations. The variants in some embodiments may further comprise H144A. The α-hemolysin variants have a decreased time to thread.

公开(公告)号：US10343350B2

公开(公告)日：2019-07-09

申请号：US15411780

申请日：2017-01-20

Applicant: Genia Technologies, Inc.

Inventor： Roger J. A. Chen ,  Randy Davis

IPC: G01N33/487 ,  C12Q1/68 ,  B29C67/20 ,  B82Y5/00 ,  C12Q1/6869 ,  B82Y10/00 ,  B82Y40/00

Abstract: Techniques for forming a nanopore in a lipid bilayer are described herein. In one example, an agitation stimulus level such as an electrical agitation stimulus is applied to a lipid bilayer wherein the agitation stimulus level tends to facilitate the formation of nanopores in the lipid bilayer. In some embodiments, a change in an electrical property of the lipid bilayer resulting from the formation of the nanopore in the lipid bilayer is detected, and a nanopore has formed in the lipid bilayer is determined based on the detected change in the lipid bilayer electrical property.

公开(公告)号：US10308918B2

公开(公告)日：2019-06-04

申请号：US15012317

申请日：2016-02-01

Applicant: Genia Technologies, Inc. ,  Roche Molecular Systems, Inc.

Inventor： Aruna Ayer ,  Arkadiusz Bibillo ,  Cleoma Renetta Arnold ,  Charles Wayan Schwab ,  Eileen Thai ,  Ilya Lederman ,  Colin Alexander McGaw ,  Tyler O'Brien Shultz ,  Barbara Eckert ,  Shawn Suko ,  Mara Boenitz-Dulat ,  Bigna Woersdoerfer ,  David Daniel Wunderlich

IPC: C12N9/12

Abstract: Described herein are variant pol6 polymerase having at least having at least two substitutions, wherein at least one of the substitutions is at position S366 of SEQ ID NO: 2. The disclosed polymerases may be coupled to alpha-hemolysin nanopores, and may be used in nanopore-based sequencing apparatuses.

公开(公告)号：US20190161795A1

公开(公告)日：2019-05-30

申请号：US16201069

申请日：2018-11-27

Applicant: Genia Technologies, Inc.

Inventor： John Foster ,  Jason Komadina

IPC: C12Q1/6874 ,  G01N27/447 ,  C23C14/34 ,  C23C14/06 ,  G01N33/487 ,  C12Q1/6869 ,  C23C14/00

Abstract: A nanopore cell includes a conductive layer. The nanopore cell further includes a titanium nitride (TiN) working electrode disposed above the conductive layer. The nanopore cell further includes insulating walls disposed above the TiN working electrode, wherein the insulating walls and the TiN working electrode form a well into which an electrolyte may be contained. In some embodiments, the TiN working electrode comprises a spongy and porous TiN working electrode that is deposited by a deposition technique with conditions tuned to deposit sparsely-spaced TiN columnar structures or columns of TiN crystals above the conductive layer.

公开(公告)号：US10301361B2

公开(公告)日：2019-05-28

申请号：US14924861

申请日：2015-10-28

Applicant: Genia Technologies, Inc.

Inventor： Michael Dorwart ,  Daniel Korenblum

IPC: C07K14/31 ,  C12Q1/6869

Abstract: Described herein are variants of alpha-hemolysin having at least one mutation selected from T12R, T12K, N17R, N17K or combinations of T12 and N17 mutations. The variants in some embodiments may further comprise H144A. The α-hemolysin variants have a decreased time to thread.

公开(公告)号：US20190154623A1

公开(公告)日：2019-05-23

申请号：US16183618

申请日：2018-11-07

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen

IPC: G01N27/327 ,  G01N33/487 ,  C12Q1/6874 ,  G01N27/447

CPC classification number: G01N27/3278 ,  C12Q1/6874 ,  G01N27/44791 ,  G01N33/48721

Abstract: A system for detecting electrical properties of a molecular complex is disclosed. The system includes an electrode electrically coupled to a molecular complex that outputs an electrical signal affected by an electrical property of the molecular complex, wherein the effect of the electrical property of the molecular complex on the electrical signal is characterized by an expected bandwidth. The system further includes an integrating amplifier circuit configured to receive the electrical signal from the electrode. The integrating amplifier circuit is further configured to selectively amplify and integrate a portion of the electrical signal over time within a predetermined bandwidth, wherein the predetermined bandwidth is selected at least in part based on the expected bandwidth.

公开(公告)号：US10266813B2

公开(公告)日：2019-04-23

申请号：US15444020

申请日：2017-02-27

Applicant: Genia Technologies, Inc.

Inventor： Aruna Ayer ,  Arkadiusz Bibillo ,  Preethi Sarvabhowman ,  Dhruti Vasudev Dalal ,  Ilya Lederman ,  Eileen Thai

IPC: C12N9/12 ,  C12N15/00 ,  C07H21/04

Abstract: Described herein are polymerase variants that are exonuclease deficient. Some variants retain the strand displacement capability comparable to the wild-type or parental polymerase. Some variants have a strand displacement capability that is improved relative to the wild-type or parental polymerase. The variants may have an extension rate that is greater than the wild-type or parental polymerase. The variants may have a waiting time that is less than the wild-type or parental polymerase.

公开(公告)号：US10227645B2

公开(公告)日：2019-03-12

申请号：US15274770

申请日：2016-09-23

Applicant: Genia Technologies, Inc.

Inventor： Timothy Kellogg Craig ,  Cynthia Ann Cech ,  Michael Dorwart ,  Liv Elisabeth Jensen ,  Marshall Winston Porter ,  Christos Tzitzilonis ,  Alexander Hyun-min Yang

IPC: C07K14/31 ,  C12Q1/6869

Abstract: Described herein are engineered alpha-hemolysin subunits having mutated oligomerization domains for assembling into heptameric nanopores in lipid bilayers.

公开(公告)号：US20190025238A1

公开(公告)日：2019-01-24

申请号：US16141658

申请日：2018-09-25

Applicant: Genia Technologies, Inc.

Inventor： Bill Maney ,  Hui Tian ,  Santiago Fernandez-Gomez

IPC: G01N27/26 ,  G01N33/487 ,  B82Y35/00

Abstract: A nanopore measurement circuit includes a first analog memory configured to store a first electrical value corresponding to a first measurement sample of a nanopore and a second analog memory configured to store a second electrical value corresponding to a second measurement sample of the nanopore. The nanopore measurement circuit also includes a measurement circuitry configured to provide an output indicating a difference between the first electrical value of the first analog memory and the second electrical value of the second analog memory.

公开(公告)号：US10174371B2

公开(公告)日：2019-01-08

申请号：US14818977

申请日：2015-08-05

Applicant: Genia Technologies, Inc.

Inventor： John Foster ,  Jason Komadina

IPC: C12Q1/6874 ,  C12Q1/6869 ,  G01N33/487 ,  C23C14/00 ,  C23C14/06 ,  C23C14/34 ,  G01N27/447

Abstract: A nanopore cell includes a conductive layer. The nanopore cell further includes a titanium nitride (TiN) working electrode disposed above the conductive layer. The nanopore cell further includes insulating walls disposed above the TiN working electrode, wherein the insulating walls and the TiN working electrode form a well into which an electrolyte may be contained. In some embodiments, the TiN working electrode comprises a spongy and porous TiN working electrode that is deposited by a deposition technique with conditions tuned to deposit sparsely-spaced TiN columnar structures or columns of TiN crystals above the conductive layer.