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64 条记录 (耗时 0.034 秒)

    Nucleotide sequences coding for the export of branched-chain amino acids, process for the isolation thereof and use thereof
    56.
    发明授权
    Nucleotide sequences coding for the export of branched-chain amino acids, process for the isolation thereof and use thereof 有权
    编码支链氨基酸输出的核苷酸序列，其分离方法及其用途

    公开(公告)号：US06841360B2

    公开(公告)日：2005-01-11

    申请号：US10608504

    申请日：2003-06-30

    申请人： Nicole Kennerknecht ,  Hermann Sahm ,  Lothar Eggeling ,  Walter Pfefferie

    发明人： Nicole Kennerknecht ,  Hermann Sahm ,  Lothar Eggeling ,  Walter Pfefferie

    IPC分类号： C12N15/09 ,  C07K14/34 ,  C12N1/21 ,  C12N15/31 ,  C12N15/54 ,  C12P13/06 ,  C12P13/08 ,  C12R1/15 ,  C12P21/06

    CPC分类号： C07K14/34 ,  C12P13/06 ,  C12P13/08

    摘要： This invention relates to isolated polynucleotides containing at least one of the polynucleotide sequences selected from the group a) polynucleotide which is at least 70% identical to a polynucleotide which codes for a polypeptide containing at least one amino acid sequence of SEQ ID no. 3 or 5, b) polynucleotide which codes for a polypeptide which contains an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID no. 3 or 5, c) polynucleotide which is complementary to the polynucleotides of a), b) or c), and d) polynucleotide containing at least 15 successive bases of the polynucleotide sequences of a), b) or c). wherein the polypeptides exhibit the biological activity of the enzymes for which the brnE or bernF (sic)gene codes and a process for the fermentative production of branched-chain L-amino acids with amplification of the stated genes.

    摘要翻译： 本发明涉及分离的多核苷酸，其含有至少一种多核苷酸序列，所述多核苷酸序列选自a组多核苷酸，所述多核苷酸序列与编码含有至少一个氨基酸序列SEQ ID NO：1的多核苷酸至少70％相同。 3或5，b）编码多肽的多核苷酸，其含有与SEQ ID No.3的氨基酸序列至少70％相同的氨基酸序列。 3或5，c）与a），b）或c）的多核苷酸互补的多核苷酸，和d）包含a），b）或c）的多核苷酸序列的至少15个连续碱基的多核苷酸。 brnE或bernF（sic）基因编码的酶的生物活性以及用所述基因扩增产生支链L-氨基酸的方法。

    Process for the fermentative preparation of L-amino acids using coryneform bacteria
    57.
    发明授权
    Process for the fermentative preparation of L-amino acids using coryneform bacteria 失效
    使用棒状细菌发酵制备L-氨基酸的方法

    公开(公告)号：US06596516B2

    公开(公告)日：2003-07-22

    申请号：US09731826

    申请日：2000-12-08

    申请人： Petra Ziegler ,  Lothar Eggeling ,  Hermann Sahm ,  Georg Thierbach ,  Walter Pfefferle

    发明人： Petra Ziegler ,  Lothar Eggeling ,  Hermann Sahm ,  Georg Thierbach ,  Walter Pfefferle

    IPC分类号： C12P1304

    CPC分类号： C12N9/1014 ,  C12P13/04 ,  C12P13/08

    摘要： A process for the preparation of L-amino acids, in which the following steps are carried out, a) fermenting the desired L-amino acid-producing bacteria in which at least the glyA gene is attenuated, in particular by removal of the natural promoter, and optionally b) concentrating the desired product in the medium or in the cells of the bacteria and c) isolating the L-amino acid, and optionally bacteria in which further genes of the biosynthesis pathway of the desired L-amino acid are additionally amplified are employed, or bacteria in which the metabolic pathways which reduce the formation of the desired L-amino acid are at least partly eliminated are employed, and nucleotide sequences of the lacI-tac-5′glyA or lacI-tac-glyA unit.

    摘要翻译： 一种制备L-氨基酸的方法，其中进行以下步骤：a）将至少所述glyA基因减毒的所需L-氨基酸产生细菌发酵，特别是通过除去天然启动子 并且任选地b）将所需产物浓缩在细菌的培养基中或细胞中，并且c）分离L-氨基酸，以及任选的细菌，其中另外扩增所需L-氨基酸的生物合成途径的其它基因的细菌 或其中代谢途径减少所需L-氨基酸的形成至少部分消除的细菌，以及lacI-tac-5'glyA或lacI-tac-glyA单元的核苷酸序列。

    Nucleotide sequences which code for the pck gene
    58.
    发明授权
    Nucleotide sequences which code for the pck gene 有权
    编码pck基因的核苷酸序列

    公开(公告)号：US06420151B1

    公开(公告)日：2002-07-16

    申请号：US09455777

    申请日：1999-12-07

    申请人： Bernard Eikmanns ,  Christian Riedel ,  Hermann Sahm ,  Bettina Mockel

    发明人： Bernard Eikmanns ,  Christian Riedel ,  Hermann Sahm ,  Bettina Mockel

    IPC分类号： C12P2106

    CPC分类号： C12N9/88 ,  C12P13/08

    摘要： Isolated polynucleotide from coryneform bacteria, comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for the polypeptide which is expressed by the pck gene contained on vector pK19mobsacB&Dgr;pck in the deposited E.coli strain DSM 13047, c) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2, d) polynucleotide which is complementary to the polynucleotides of a), b) or c) and e) polynucleotide comprising at least 15 successive bases of the polynucleotide sequence of a), b), c) or d).

    摘要翻译： 来自棒状细菌的分离的多核苷酸，包含选自以下的多核苷酸序列：a）与编码包含SEQ ID No.2的氨基酸序列的多肽的多核苷酸的程度相同的多核苷酸， b）与编码由沉积的大肠杆菌菌株DSM 13047中载体pK19mobsacBDELTApck上包含的pck基因表达的多肽的多核苷酸的程度至少70％相同的多核苷酸，c）编码 多肽，其包含与SEQ ID No.2的氨基酸序列至少70％相同的氨基酸序列，d）与a），b）或c）和ee）的多核苷酸互补的多核苷酸， 包含a），b），c）或d）的多核苷酸序列的至少15个连续碱基的多核苷酸。