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    Chemical temperature control
    51.
    发明授权
    Chemical temperature control 有权
    化学温度控制

    公开(公告)号：US08431387B2

    公开(公告)日：2013-04-30

    申请号：US12134965

    申请日：2008-06-06

    申请人： Paul Donald LaBarre ,  Jay Lewis Gerlach ,  Bernhard Hans Weigl ,  Gonzalo Jose Domingo-Villegas

    发明人： Paul Donald LaBarre ,  Jay Lewis Gerlach ,  Bernhard Hans Weigl ,  Gonzalo Jose Domingo-Villegas

    IPC分类号： C12M3/00 ,  C12M1/34 ,  C12M1/00 ,  B06B1/00 ,  A61F7/00 ,  A61F7/08 ,  F24J1/00 ,  F24J3/00

    CPC分类号： C12Q1/686 ,  B01L3/5027 ,  B01L3/50273 ,  B01L7/00 ,  B01L7/525 ,  B01L2300/0663 ,  B01L2300/0816 ,  B01L2300/088 ,  B01L2300/1855 ,  B01L2300/1877 ,  B01L2400/0406 ,  B01L2400/0442 ,  B01L2400/0445 ,  B01L2400/0478 ,  B01L2400/0487

    摘要： Exothermic and/or endothermic chemical reactions in combination with phase change materials can produce output temperature(s) within strict tolerances without requiring expensive and complicated external equipment to generate and maintain an output temperature. Similarly, an exothermic phase change material, which generates heat as a consequence of crystallizing a supercooled liquid, can generate heat at a constant temperature, without requiring expensive and complicated external equipment, as a consequence of the liquid form of the exothermic phase change material being in equilibrium with the solid form of the exothermic phase change material. Numerous biological and chemical processes and/or diagnostic devices require a constant temperature or temperatures for set periods of time. An example completely non-instrumented diagnostic platform based on nucleic acid amplification is described, which is particularly suited for use in developing countries that may not have access to expensive and complicated external equipment.

    摘要翻译： 与相变材料相结合的放热和/或吸热化学反应可以在严格的公差范围内产生输出温度，而不需要昂贵和复杂的外部设备来产生和保持输出温度。 类似地，作为结晶过冷液体的结果产生热量的放热相变材料可以在恒定温度下产生热量，而不需要昂贵且复杂的外部设备，因为放热相变材料的液体形式是 与固体形式的放热相变材料平衡。 许多生物和化学过程和/或诊断装置需要恒定的温度或温度来设定一段时间。 描述了基于核酸扩增的完全非仪器化诊断平台的示例，其特别适用于可能无法访问昂贵且复杂的外部设备的发展中国家。

    METHOD AND APPARATUS FOR AMPLIFICATION OF NUCLEIC ACID SEQUENCES BY USING THERMAL CONVECTION
    53.
    发明申请
    METHOD AND APPARATUS FOR AMPLIFICATION OF NUCLEIC ACID SEQUENCES BY USING THERMAL CONVECTION 审中-公开
    通过使用热对流来放大核酸序列的方法和装置

    公开(公告)号：US20120021463A1

    公开(公告)日：2012-01-26

    申请号：US13252039

    申请日：2011-10-03

    申请人： Hyun Jin Hwang ,  Jeong Hee Kim ,  Kyunghoon Jeong

    发明人： Hyun Jin Hwang ,  Jeong Hee Kim ,  Kyunghoon Jeong

    IPC分类号： C12P19/34 ,  C12M1/00

    CPC分类号： C12P19/34 ,  B01L7/525 ,  B01L2300/1838 ,  B01L2300/1883 ,  B01L2400/0442 ,  B01L2400/0445

    摘要： The present invention provides a nucleic acid sequence amplification method and apparatuses thereof that are simple in the design and easy to miniaturize and integrate into complex apparatuses, with capability of using DNA polymerases that are not thermostable. In the present invention, a plurality of heat sources are combined to supply or remove heat from specific regions of the sample such that a specific spatial temperature distribution is maintained inside the sample by locating a relatively high temperature region lower in height than a relatively low temperature region.

    摘要翻译： 本发明提供一种设计简单，易于小型化并且整合到复杂装置中的核酸序列扩增方法及其装置，具有使用不耐热的DNA聚合酶的能力。 在本发明中，组合多个热源以从样品的特定区域供应或除去热量，使得通过将相对高的温度区域定位在比相对低的温度 地区。

    METHOD AND APPARATUS FOR AMPLIFICATION OF NUCLEIC ACID SEQUENCES BY USING THERMAL CONVECTION
    54.
    发明申请
    METHOD AND APPARATUS FOR AMPLIFICATION OF NUCLEIC ACID SEQUENCES BY USING THERMAL CONVECTION 有权
    通过使用热对流来放大核酸序列的方法和装置

    公开(公告)号：US20100285536A1

    公开(公告)日：2010-11-11

    申请号：US12610245

    申请日：2009-10-30

    申请人： Hyun Jin Hwang ,  Jeong Hee Kim ,  Kyunghoon Jeong

    发明人： Hyun Jin Hwang ,  Jeong Hee Kim ,  Kyunghoon Jeong

    IPC分类号： C12P19/34 ,  C12M1/00 ,  C12M1/02

    CPC分类号： C12P19/34 ,  B01L7/525 ,  B01L2300/1838 ,  B01L2300/1883 ,  B01L2400/0442 ,  B01L2400/0445

    摘要： The present invention provides a nucleic acid sequence amplification method and apparatuses thereof that are simple in the design and easy to miniaturize and integrate into complex apparatuses, with capability of using DNA polymerases that are not thermostable. In the present invention, a plurality of heat sources are combined to supply or remove heat from specific regions of the sample such that a specific spatial temperature distribution is maintained inside the sample by locating a relatively high temperature region lower in height than a relatively low temperature region.

    摘要翻译： 本发明提供一种设计简单，易于小型化并且整合到复杂装置中的核酸序列扩增方法及其装置，具有使用不耐热的DNA聚合酶的能力。 在本发明中，组合多个热源以从样品的特定区域供应或除去热量，使得通过将相对高的温度区域定位在比相对低的温度 地区。

    METHOD AND APPARATUS FOR AMPLIFICATION OF NUCLEIC ACID SEQUENCES USING IMMOBILIZED DNA POLYMERASE
    56.
    发明申请
    METHOD AND APPARATUS FOR AMPLIFICATION OF NUCLEIC ACID SEQUENCES USING IMMOBILIZED DNA POLYMERASE 有权
    使用固定化DNA聚合酶扩增核酸序列的方法和装置

    公开(公告)号：US20100086975A1

    公开(公告)日：2010-04-08

    申请号：US12351764

    申请日：2009-01-09

    申请人： Hyun Jin Hwang ,  Jeong Hee Kim ,  Kyunghoon Jeong

    发明人： Hyun Jin Hwang ,  Jeong Hee Kim ,  Kyunghoon Jeong

    IPC分类号： C12P19/34 ,  C12M1/38

    CPC分类号： B01L7/525 ,  B01L2300/1838 ,  B01L2300/1883 ,  B01L2400/0442 ,  B01L2400/0445

    摘要： The present invention generally relates to methods and apparatuses for amplifying nucleic acid sequences using immobilized DNA polymerase. More particularly, it relates to methods and apparatuses useful for amplifying target nucleic acid sequences by forming a plurality of reaction regions in which polymerase chain reaction (PCR) can occur, positioning immobilized DNA polymerase in a specific reaction region, and circulating DNA through the reaction regions. The present invention provides those methods and apparatuses that allow simple separation and recovery of the DNA polymerase after the amplification, that can be operated not only with thermostable DNA polymerases but also with non-thermostable DNA polymerases, and that are simpler in their designs and processes so that they can be readily integrated into complex devices such as Lab-on-a-chip.

    摘要翻译： 本发明一般涉及使用固定化DNA聚合酶扩增核酸序列的方法和装置。 更具体地说，涉及通过形成能够发生聚合酶链式反应（PCR）的多个反应区域扩增靶核酸序列的方法和装置，将固定的DNA聚合酶定位在特定的反应区域中，并通过反应循环DNA 地区。 本发明提供了在扩增后能够简单地分离和回收DNA聚合酶的方法和装置，其不仅可以用热稳定的DNA聚合酶而且可以用非热稳定的DNA聚合酶来操作，并且它们的设计和过程更简单 使得它们可以容易地集成到诸如片上实验室的复杂器件中。

    MICROFLUIDIC CHIP
    57.
    发明申请
    MICROFLUIDIC CHIP 审中-公开
    微流感芯片

    公开(公告)号：US20080153152A1

    公开(公告)日：2008-06-26

    申请号：US11943380

    申请日：2007-11-20

    申请人： Akira Wakabayashi ,  Hideyuki Karaki ,  Yoshihiro Sawayashiki ,  Kota Kato ,  Yoshihide Iwaki

    发明人： Akira Wakabayashi ,  Hideyuki Karaki ,  Yoshihiro Sawayashiki ,  Kota Kato ,  Yoshihide Iwaki

    IPC分类号： C12M1/00

    CPC分类号： B01F1/0022 ,  B01F5/0646 ,  B01F5/0655 ,  B01F13/0059 ,  B01F13/1013 ,  B01F13/1027 ,  B01L3/502707 ,  B01L3/502723 ,  B01L7/525 ,  B01L2200/0684 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/0445 ,  B01L2400/0487 ,  B01L2400/0688

    摘要： A microfluidic chip, includes: a first port for inputting: a sample liquid; and a first liquid; a second port for inputting a second liquid; a third port for supplying air pressure; a first channel (A) for mixing the sample liquid and the first liquid to generate a first mixed liquid; a second channel (B) for beating the first mixed liquid; a third channel (C) for allowing the second liquid to converge into the first mixed liquid to generate a second mixed liquid; a fourth channel (D) installing a first solid; a fifth channel (E) for promoting mixing of the first solid; a plurality of sixth channels (F) each having a second solid; and a seventh channel (G), which connects the fifth channel (E) and the plurality of sixth channels (F), for dispensing a fixed quantity of the second mixed liquid to each of the plurality of sixth channels (F).

    摘要翻译： 一种微流体芯片，包括：第一端口，用于输入：样品液体; 和第一液体; 用于输入第二液体的第二端口; 用于提供空气压力的第三个端口; 用于混合样品液体和第一液体以产生第一混合液体的第一通道（A）; 用于打浆第一混合液体的第二通道（B）; 第三通道（C），用于允许第二液体会聚到第一混合液体中以产生第二混合液体; 第四通道（D），安装第一固体; 用于促进第一固体的混合的第五通道（E）; 多个第六通道（F），每个具有第二固体; 以及连接第五通道（E）和多个第六通道（F）的第七通道（G），用于将固定量的第二混合液体分配到多个第六通道（F）中的每一个。