公开(公告)号：US20080153152A1

公开(公告)日：2008-06-26

申请号：US11943380

申请日：2007-11-20

申请人： Akira Wakabayashi ,  Hideyuki Karaki ,  Yoshihiro Sawayashiki ,  Kota Kato ,  Yoshihide Iwaki

发明人： Akira Wakabayashi ,  Hideyuki Karaki ,  Yoshihiro Sawayashiki ,  Kota Kato ,  Yoshihide Iwaki

IPC分类号： C12M1/00

CPC分类号： B01F1/0022 ,  B01F5/0646 ,  B01F5/0655 ,  B01F13/0059 ,  B01F13/1013 ,  B01F13/1027 ,  B01L3/502707 ,  B01L3/502723 ,  B01L7/525 ,  B01L2200/0684 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/0445 ,  B01L2400/0487 ,  B01L2400/0688

摘要： A microfluidic chip, includes: a first port for inputting: a sample liquid; and a first liquid; a second port for inputting a second liquid; a third port for supplying air pressure; a first channel (A) for mixing the sample liquid and the first liquid to generate a first mixed liquid; a second channel (B) for beating the first mixed liquid; a third channel (C) for allowing the second liquid to converge into the first mixed liquid to generate a second mixed liquid; a fourth channel (D) installing a first solid; a fifth channel (E) for promoting mixing of the first solid; a plurality of sixth channels (F) each having a second solid; and a seventh channel (G), which connects the fifth channel (E) and the plurality of sixth channels (F), for dispensing a fixed quantity of the second mixed liquid to each of the plurality of sixth channels (F).

摘要翻译： 一种微流体芯片，包括：第一端口，用于输入：样品液体; 和第一液体; 用于输入第二液体的第二端口; 用于提供空气压力的第三个端口; 用于混合样品液体和第一液体以产生第一混合液体的第一通道（A）; 用于打浆第一混合液体的第二通道（B）; 第三通道（C），用于允许第二液体会聚到第一混合液体中以产生第二混合液体; 第四通道（D），安装第一固体; 用于促进第一固体的混合的第五通道（E）; 多个第六通道（F），每个具有第二固体; 以及连接第五通道（E）和多个第六通道（F）的第七通道（G），用于将固定量的第二混合液体分配到多个第六通道（F）中的每一个。

公开(公告)号：US20080130402A1

公开(公告)日：2008-06-05

申请号：US11940939

申请日：2007-11-15

申请人： Hideyuki KARAKI ,  Yoshihiro Sawayashiki ,  Akira Wakabayashi ,  Yoshihide Iwaki

发明人： Hideyuki KARAKI ,  Yoshihiro Sawayashiki ,  Akira Wakabayashi ,  Yoshihide Iwaki

IPC分类号： B01F15/02 ,  B01F15/00

CPC分类号： B01F13/0059 ,  B01F15/0203 ,  B01F15/0232 ,  B01L3/502723 ,  B01L3/50273 ,  B01L3/502738 ,  B01L2200/0621 ,  B01L2200/0684 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/161 ,  B01L2400/0406 ,  B01L2400/0487 ,  B01L2400/049 ,  B01L2400/0688

摘要： A microchannel chip, includes: a first channel where a first liquid is transported from one end side to an opposite end side; a port section to which a second liquid is supplied from outside for accumulating the second liquid; and a second channel connecting the first channel and the port section through a first opening provided in a side of the first channel and a second opening provided in the port section, wherein the second channel checks flowing out of the second liquid accumulated in the port section to the first channel by a Laplace pressure valve until the first liquid arrives at the first opening, and the second channel converges the second liquid into the first liquid after the first liquid reaches the first opening, and a converging device using the same.

摘要翻译： 微通道芯片包括：第一通道，其中第一液体从一端侧输送到相对端侧; 从外部供给第二液体以积聚第二液体的端口部分; 以及第二通道，其通过设置在所述第一通道的侧面中的第一开口和设置在所述端口部分中的第二开口连接所述第一通道和所述端口部分，其中所述第二通道检查从积存在所述端口部分中的所述第二液体流出 通过拉普拉斯压力阀向第一通道供应直到第一液体到达第一开口，并且第二通道在第一液体到达第一开口之后将第二液体收敛到第一液体中，以及使用该第一流体的会聚装置。

公开(公告)号：US20080153155A1

公开(公告)日：2008-06-26

申请号：US11943353

申请日：2007-11-20

申请人： Kota KATO ,  Yoshihide Iwaki ,  Akira Wakabayashi ,  Hideyuki Karaki

发明人： Kota KATO ,  Yoshihide Iwaki ,  Akira Wakabayashi ,  Hideyuki Karaki

IPC分类号： C12M1/00 ,  B01J19/00

CPC分类号： G01N33/54366 ,  B01L3/502707 ,  B01L7/52 ,  B01L2200/16 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/1805 ,  B01L2400/0487 ,  B01L2400/0677 ,  G01N33/548

摘要： A microchannel chip for introducing an inspected liquid, includes: a micro channel; and a reagent that is mixed with a heat soluble binder and is carried at a predetermined position in the micro channel, wherein dissolution of the reagent is promoted at the predetermined position as temperature of the inspected liquid rises from temperature when the inspected liquid is introduced.

摘要翻译： 用于引入检查液体的微通道芯片包括：微通道; 以及与热溶性粘合剂混合并在微通道中的预定位置携带的试剂，其中当检查液体的温度从检查液体导入时的温度升高时，试剂的溶解在预定位置被促进。

公开(公告)号：US20080152543A1

公开(公告)日：2008-06-26

申请号：US11943370

申请日：2007-11-20

申请人： Hideyuki KARAKI ,  Tatsuo Fujikura ,  Akira Wakabayashi ,  Yoshihide Iwaki

发明人： Hideyuki KARAKI ,  Tatsuo Fujikura ,  Akira Wakabayashi ,  Yoshihide Iwaki

IPC分类号： G01N21/64 ,  B01J19/00 ,  G05D23/00 ,  G05D23/13

CPC分类号： G05D23/1919 ,  B01L3/5027 ,  B01L3/502746 ,  B01L7/52 ,  B01L2200/0684 ,  B01L2200/142 ,  B01L2200/147 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2400/0487 ,  G01N21/0332 ,  G01N21/05 ,  G01N21/272 ,  G01N21/6486 ,  G01N2021/0325 ,  G01N2021/0346

摘要： A temperature regulation method of a microfluidic chip for controlling a temperature of a liquid accumulated in a reaction channel formed in a flat plate of the microfluidic chip, the method includes: heating the reaction channel to a reaction temperature; and at the same time as the reaction channel is heated to the reaction temperature, keeping a first channel communicating with one end of the reaction channel and a second channel communicating with an opposite end of the reaction channel at the same temperature different from the reaction temperature by heating or cooling.

摘要翻译： 一种微流控芯片的温度调节方法，用于控制积聚在形成于微流体芯片的平板中的反应通道中的液体的温度，该方法包括：将反应通道加热至反应温度; 并且在反应通道被加热到反应温度的同时，保持与反应通道的一端连通的第一通道和与反应通道的相反端在与反应温度不同的相同温度下连通的第二通道 通过加热或冷却。

公开(公告)号：US20120179384A1

公开(公告)日：2012-07-12

申请号：US13395509

申请日：2010-09-09

申请人： Masayuki Kuramitsu ,  Yoshihide Iwaki ,  Dai Ujihara

发明人： Masayuki Kuramitsu ,  Yoshihide Iwaki ,  Dai Ujihara

IPC分类号： G06F19/20

CPC分类号： C12Q1/6837 ,  C12Q2600/156 ,  G16B25/00

摘要： There is provided a method for analyzing nucleic acid mutation using an array comparative genomic hybridization technique, which reduces the false positive and the false negative and improves the reliability of the analysis results. A method for analyzing nucleic acid mutation using array comparative genomic hybridization technique comprising: [(a) a step of bringing a plurality of labeled sample nucleic acids one by one into contact with the plural same probe nucleic acid sets], [(b) a step of obtaining label intensities], [(c) a step of determining whether each piece of comparison values falls within a prescribed numerical value range or not], and [(d) a step of comparing whether the number of the comparison values exceeds a prescribed number or not and, in the case of exceeding the prescribed number, judging the spot positive].

摘要翻译： 提供了使用阵列比较基因组杂交技术分析核酸突变的方法，其减少了假阳性和假阴性，并提高了分析结果的可靠性。 使用阵列比较基因组杂交技术分析核酸突变的方法，包括：（a）使多个标记的样品核酸逐个与多个相同的探针核酸组相接触的步骤]，[（b） 获得标签强度的步骤]，[（c）确定每个比较值是否落在规定的数值范围内的步骤]，[（d）比较比较值的数量是否超过 在规定数量的情况下，判定现场为正]。

公开(公告)号：US20100047794A1

公开(公告)日：2010-02-25

申请号：US12471817

申请日：2009-05-26

申请人： Hayato Miyoshi ,  Yoshihide Iwaki ,  Toshihiro Mori

发明人： Hayato Miyoshi ,  Yoshihide Iwaki ,  Toshihiro Mori

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6844 ,  C12Q1/6858 ,  C12Q2531/119 ,  C12Q2531/101

摘要： It is an object of the present invention to provide a method for discriminating between nucleic acid sequences with high accuracy by utilizing a method for specifically amplifying nucleic acid sequences under isothermal conditions. The present invention provides a method for discriminating between the nucleotide sequence of a first nucleic acid and the nucleotide sequence of a second nucleic acid by a nucleic acid amplification method that is performed under substantially isothermal conditions, wherein (1) at least one type of oligonucleotide (hereinafter “primer”) substantially complementary to the first nucleic acid, and (2) at least one type of oligonucleic acid (hereinafter “mask oligo”) that is designed such that it hybridizes to the nucleotide sequence portions of the first nucleic acid and the second nucleic acid to be discriminated, such that it is more complementary to the second nucleic acid than to the first nucleic acid, and such that it does not become an origin of an elongation reaction with polymerase, are used, the method being characterized in that a portion of the primer and a portion of the mask oligo hybridize to the same regions on the first nucleic acid and the second nucleic acid.

摘要翻译： 本发明的目的是提供一种通过利用在等温条件下特异性扩增核酸序列的方法来高精度地区分核酸序列的方法。 本发明提供了通过在基本上等温条件下进行的核酸扩增方法来区分第一核酸的核苷酸序列和第二核酸的核苷酸序列的方法，其中（1）至少一种类型的寡核苷酸 （以下简称为“引物”），和（2）至少一种低聚核酸（以下称为“掩模寡核苷酸”），其被设计为与第一核酸的核苷酸序列部分杂交， 要被鉴别的第二核酸，使得其比第一核酸与第二核酸更互补，并且使其不成为与聚合酶的延伸反应的起源，所述方法的特征在于 引物的一部分和掩模寡核苷酸的一部分与第一核酸和第二核酸上的相同区域杂交。

公开(公告)号：US07410613B2

公开(公告)日：2008-08-12

申请号：US10397376

申请日：2003-03-27

申请人： Yoshihide Iwaki ,  Kentarou Nakamura ,  Hideaki Tanaka ,  Yoshiki Sakaino ,  Kaoru Terashima ,  Hitoshi Shimizu

发明人： Yoshihide Iwaki ,  Kentarou Nakamura ,  Hideaki Tanaka ,  Yoshiki Sakaino ,  Kaoru Terashima ,  Hitoshi Shimizu

IPC分类号： G01N21/78

CPC分类号： G01N21/78 ,  G01N21/5907

摘要： A humoral testing apparatus in which a measuring light is irradiated to a reagent area forming a color as a result of a reaction and an optical density of the reagent area is detected by a detecting operation of light intensity of the reflected light. The humoral testing apparatus enables a humoral test to be performed accurately in cases where nonuniformity occurs with the reaction of a reagent with a humoral sample within the reagent area, or in cases where fine dust is present within each detecting spot in the reagent areas. The light reflected from the reagent areas is detected with a two-dimensional photodetector. The independent light intensity detecting operations are performed with respect to the subareas of the reagent area of a reagent layer. A photo detection signal S, which represents the intensity of the reflected light is statistically processed with a signal processing section 51.

摘要翻译： 通过反射光的光强度的检测操作来检测其中测量光照射到由于反应而形成颜色的试剂区域和试剂区域的光密度的体液测试装置。 体液检测装置能够在试剂区域内的试剂与体液样品的反应发生不均匀的情况下，或者在试剂区域的每个检测点内存在微细粉尘的情况下，能够准确地进行体液检查。 用二维光电检测器检测从试剂区反射的光。 针对试剂层的试剂区域的子区域进行独立的光强度检测动作。 用信号处理部分51对表示反射光强度的光检测信号S进行统计处理。

公开(公告)号：US20080044921A1

公开(公告)日：2008-02-21

申请号：US11822271

申请日：2007-07-03

申请人： Yoshihide Iwaki ,  Toshihiro Mori

发明人： Yoshihide Iwaki ,  Toshihiro Mori

IPC分类号： G01N33/48 ,  C07H21/00

CPC分类号： C12Q1/6853 ,  C12Q1/6858 ,  Y10T436/143333 ,  C12Q2525/301 ,  C12Q2525/161

摘要： A primer for amplifying a target nucleic acid sequence, comprises: a sequence region (a) complementary to a sequence region (a′) in the target nucleic acid sequence; and a sequence region (b) having a sequence complementary to a partial sequence of the sequence region (a), in this order from a 3′ terminal side to a 5′ terminal side of the primer.

摘要翻译： 用于扩增靶核酸序列的引物包括：与靶核酸序列中的序列区（a'）互补的序列区（a）; 和具有与序列区（a）的部分序列互补的序列的序列区（b），从该引物的3'末端侧至5'末端侧。

公开(公告)号：US20090170096A1

公开(公告)日：2009-07-02

申请号：US12179098

申请日：2008-07-24

申请人： Hayato MIYOSHI ,  Yoshihide Iwaki ,  Toshihiro Mori

发明人： Hayato MIYOSHI ,  Yoshihide Iwaki ,  Toshihiro Mori

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6853 ,  C12Q2531/119 ,  C12Q2527/125 ,  C12Q2521/101

摘要： An object to be achieved by the present invention is to provide a nucleic acid amplification method by which a nucleic acid can be amplified substantially isothermally using oligonucleotide primers and DNA polymerase capable of strand displacement. The present invention provides a nucleic acid amplification method which comprises performing substantially isothermal incubation of a reaction solution containing at least one type of deoxynucleotide triphosphate, at least one type of DNA polymerase having strand displacement activity, a divalent cation, at least 0.01% or more surfactant, at least two types of oligonucleotide primer, and the nucleic acid fragment as a template so as to perform a polymerase reaction that initiates from the 3′ end of the primer and thus amplifying the nucleic acid fragment.

摘要翻译： 本发明要实现的目的是提供一种使用寡核苷酸引物和能够进行链置换的DNA聚合酶基本上等温扩增核酸的核酸扩增方法。 本发明提供了一种核酸扩增方法，其包括对含有至少一种类型的脱氧核苷酸三磷酸的反应溶液进行基本上恒温培养，至少一种具有链置换活性的DNA聚合酶，二价阳离子，至少0​​.01％或更多 表面活性剂，至少两种类型的寡核苷酸引物和核酸片段作为模板，从而进行从引物的3'末端引发并因此扩增核酸片段的聚合酶反应。

公开(公告)号：US20090162856A1

公开(公告)日：2009-06-25

申请号：US12265629

申请日：2008-11-05

申请人： Hayato Miyoshi ,  Yoshihide Iwaki ,  Toshihiro Mori

发明人： Hayato Miyoshi ,  Yoshihide Iwaki ,  Toshihiro Mori

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/686

摘要： It is an object of the present invention to provide a method for rapid, convenient, and highly sensitive detection of trace RNA wherein a risk of contamination is low. The present invention provides a method for amplification of nucleic acid which comprises the steps of: (i) allowing a reverse transcriptase to act on RNA so as to produce a nucleic acid fragment; and (ii) performing substantially isothermal incubation of a reaction solution containing at least one type of deoxynucleotide triphosphate, at least one type of DNA polymerase having strand displacement activity, a divalent cation, a surfactant accounting for at least 0.01% of the solution, at least two types of oligonucleotide primers, and the nucleic acid fragment as a template obtained in the step (i) so as to perform a polymerase reaction that is initiated from the 3′ ends of the primers and thus amplify the nucleic acid fragment.

摘要翻译： 本发明的目的是提供一种用于快速，方便和高灵敏度地检测痕量RNA的方法，其中污染的风险低。 本发明提供核酸扩增方法，其包括以下步骤：（i）使逆转录酶作用于RNA以产生核酸片段; 和（ii）对包含至少一种类型的脱氧核苷酸三磷酸的反应溶液，至少一种具有链置换活性的DNA聚合酶，二价阳离子，占该溶液的至少0.01％的表面活性剂进行基本上等温温育 至少两种寡核苷酸引物，以及作为在步骤（i）中获得的模板的核酸片段，以进行从引物的3'末端引发的聚合酶反应，从而扩增核酸片段。