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    COMPOSITIONS AND METHODS FOR PRODUCING CHEMICALS AND DERIVATIVES THEREOF
    51.
    发明申请
    COMPOSITIONS AND METHODS FOR PRODUCING CHEMICALS AND DERIVATIVES THEREOF 有权
    用于生产化学品及其衍生物的组合物和方法

    公开(公告)号：US20140206047A1

    公开(公告)日：2014-07-24

    申请号：US14222453

    申请日：2014-03-21

    申请人： Synthetic Genomics, Inc.

    发明人： Spiros Kambourakis ,  Benjamin M. Griffin ,  Kevin V. Martin

    IPC分类号： C12P7/58 ,  C12P7/42 ,  C12P17/04

    CPC分类号： C12P7/58 ,  C07D307/33 ,  C07D309/30 ,  C12N15/52 ,  C12P7/42

    摘要： The present invention provides methods for producing a product of one or more enzymatic pathways. The pathways used in the methods of the invention involve one or more conversion steps such as, for example, an enzymatic conversion of guluronic acid into D-glucarate (Step 7); an enzymatic conversion of 5-ketogluconate (5-KGA) into L-Iduronic acid (Step 15); an enzymatic conversion of L-Iduronic acid into Idaric acid Step 7b); and an enzymatic conversion of 5-ketocluconate into 4,6-dihydroxy 2,5-diketo hexanoate (2,5-DDH) (Step 16). In some embodiments the methods of the invention produce 2,5-furandicarboxylic acid (FDCA) as a product. The methods include both enzymatic and chemical conversions as steps. Various pathways are also provided for converting glucose into 5-dehdyro-4-deoxy-glucarate (DDG), and for converting glucose into 2,5-furandicarboxylic acid (FDCA). The methods also involve the use of engineered enzymes that perform reactions with high specificity and efficiency. Additional products that can be produce include metabolic products such as, but not limited to, guluronic acid, L-iduronic acid, idaric acid, glucaric acid. Any of the products can be produced using glucose as a substrate or using any intermediate in any of the methods or pathways of the invention.

    摘要翻译： 本发明提供了生产一种或多种酶途径的产物的方法。 用于本发明方法的途径包括一个或多个转化步骤，例如将古罗糖醛酸酶促转化成D-葡糖二酸（步骤7）; 将5-酮葡萄糖酸（5-KGA）酶促转化成L-阿魏酸（步骤15）; 步骤7b）中将L-阿魏酸转化成Idaric酸的酶促转化; 并将5-酮基葡萄糖酸酯酶促转化成4,6-二羟基2,5-二酮己酸酯（2,5-DDH）（步骤16）。 在一些实施方案中，本发明的方法产生作为产物的2,5-呋喃二羧酸（FDCA）。 所述方法包括作为步骤的酶和化学转化。 还提供了将葡萄糖转化为5-脱氢-4-脱氧葡糖二酸（DDG）和将葡萄糖转化为2,5-呋喃二羧酸（FDCA）的各种途径。 该方法还涉及使用以高特异性和高效率进行反应的工程化酶。 可以生产的其它产品包括代谢产物，例如但不限于古洛糖醛酸，L-艾杜糖醛酸，id酸，葡糖二酸。 可以使用葡萄糖作为底物或在本发明的任何方法或途径中使用任何中间体来生产任何产物。

    MULTIMERIC OXIDOREDUCTASES
    52.
    发明申请
    MULTIMERIC OXIDOREDUCTASES 有权
    多种氧化剂

    公开(公告)号：US20140170718A1

    公开(公告)日：2014-06-19

    申请号：US14076945

    申请日：2013-11-11

    申请人： Danisco US Inc.

    发明人： Robert M. CALDWELL ,  M. Harunur RASHID ,  Fernando VALLE

    IPC分类号： C12P7/58 ,  C12P7/60

    CPC分类号： C12P7/58 ,  C12N9/0004 ,  C12N9/002 ,  C12N15/74 ,  C12N15/78 ,  C12P7/60

    摘要： The present invention concerns multimeric oxidoreductase complexes which function in the enzymatic conversion of a carbon substrate, said complexes having a dehydrogenase subunit and a cytochrome C subunit. The invention further relates to polynucleotides coding for the multimeric complexes and methods of use thereof.

    摘要翻译： 本发明涉及在碳底物的酶促转化中起作用的多聚体氧化还原酶复合物，所述复合物具有脱氢酶亚基和细胞色素C亚基。 本发明还涉及编码多聚体复合物的多核苷酸及其使用方法。

    Isolated fungal promoters and gene transcription terminators and methods of protein and chemical production in a fungus
    53.
    发明授权
    Isolated fungal promoters and gene transcription terminators and methods of protein and chemical production in a fungus 有权
    分离的真菌启动子和基因转录终止子以及真菌中蛋白质和化学生产的方法

    公开(公告)号：US08735562B2

    公开(公告)日：2014-05-27

    申请号：US12257261

    申请日：2008-10-23

    申请人： Ziyu Dai ,  Linda L. Lasure ,  Jon K. Magnuson

    发明人： Ziyu Dai ,  Linda L. Lasure ,  Jon K. Magnuson

    IPC分类号： C07H21/04 ,  C12N15/63

    CPC分类号： C12P7/44 ,  C07K14/38 ,  C12N15/80 ,  C12P7/46 ,  C12P7/48 ,  C12P7/56 ,  C12P7/58 ,  C12P17/06 ,  C12P21/005 ,  C12P35/00 ,  C12P37/00

    摘要： The present invention encompasses isolated gene regulatory elements and gene transcription terminators that are differentially expressed in a native fungus exhibiting a first morphology relative to the native fungus exhibiting a second morphology. The invention also encompasses a method of utilizing a fungus for protein or chemical production. A transformed fungus is produced by transforming a fungus with a recombinant polynucleotide molecule. The recombinant polynucleotide molecule contains an isolated polynucleotide sequence linked operably to another molecule comprising a coding region of a gene of interest. The gene regulatory element and gene transcription terminator may temporally and spatially regulate expression of particular genes for optimum production of compounds of interest in a transgenic fungus.

    摘要翻译： 本发明包括在天然真菌中差异表达的分离的基因调控元件和基因转录终止子，其表现出相对于表现出第二形态的天然真菌的第一形态。 本发明还包括利用真菌进行蛋白质或化学生产的方法。 通过用重组多核苷酸分子转化真菌产生转化的真菌。 重组多核苷酸分子含有与包含感兴趣的基因的编码区的另一个分子可操作地连接的分离的多核苷酸序列。 基因调控元件和基因转录终止子可以在时间上和空间上调节特定基因的表达，以在转基因真菌中最佳产生感兴趣的化合物。

    GLUCO-OLIGOSACCHARIDE OXIDASES FROM ACREMONIUM STRICTUM AND USES THEREOF
    54.
    发明申请
    GLUCO-OLIGOSACCHARIDE OXIDASES FROM ACREMONIUM STRICTUM AND USES THEREOF 审中-公开
    来自ACREMONIUM STRICTUM的GLUCO-OLIGOSACCHARIDE氧化物及其用途

    公开(公告)号：US20140057332A1

    公开(公告)日：2014-02-27

    申请号：US14002002

    申请日：2012-02-28

    申请人： Maryam Foumani ,  Thu V. Vuong ,  Emma R. Master

    发明人： Maryam Foumani ,  Thu V. Vuong ,  Emma R. Master

    IPC分类号： C12N9/04

    CPC分类号： C12N9/0006 ,  C12P7/58 ,  C12P19/00 ,  C12P19/04 ,  C12Y101/99

    摘要： This invention provides a description of novel polypeptides and nucleotide sequences having gluco-oligosaccharide oxidase (GOOX) activity. The polypeptides of the invention can be used for enzymatic processes that modify carbohydrates from wood fiber. These polypeptides can be used in the oxidation of C6 and C5 mono- and oligomeric sugars. These polypeptides can also be used for the oxidation of glucose, xylose, galactose, NAG, xylo-oligosaccharides, cello-oligosaccharides. The novel polypeptides of the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts.

    摘要翻译： 本发明提供具有葡糖 - 寡糖氧化酶（GOOX）活性的新型多肽和核苷酸序列的描述。 本发明的多肽可用于从木纤维改性碳水化合物的酶促方法。 这些多肽可用于C6和C5单糖和低聚糖的氧化。 这些多肽也可以用于葡萄糖，木糖，半乳糖，NAG，低聚木糖，低聚低聚糖的氧化。 本发明的新颖多肽可用于多种药物，农业和工业环境中。

    Methods and materials for the production of shikimic acid
    57.
    发明授权
    Methods and materials for the production of shikimic acid 有权
    用于生产莽草酸的方法和材料

    公开(公告)号：US08372621B2

    公开(公告)日：2013-02-12

    申请号：US12839964

    申请日：2010-07-20

    申请人： John W. Frost

    发明人： John W. Frost

    IPC分类号： C12N9/04 ,  C12N9/88

    CPC分类号： C12N9/0006 ,  C12N9/88 ,  C12P7/26 ,  C12P7/42 ,  C12P7/58 ,  C12P9/00

    摘要： Novel enzymes and novel enzymatic pathways for the pyruvate-based synthesis of shikimate or at least one intermediate thereto or derivative thereof, nucleic acids encoding the enzymes, cells transformed therewith, and kits containing said enzymes, cells, or nucleic acid. A KDPGal aldolase is used to perform condensation of pyruvate with D-erythrose 4-phosphate to form 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP); a 3-dehydroquinate synthase is used to convert the DAHP to 3-dehydroquinate (DHQ); DHQ dehydratase can then convert DHQ to the key shikimate intermediate, 3-dehydroshikimate.

    摘要翻译： 用于基于丙酮酸的合成莽草酸或其至少一种中间体或其衍生物的新型酶和新的酶途径，编码酶的核酸，用其转化的细胞，以及含有所述酶，细胞或核酸的试剂盒。 使用KDPGal醛缩酶进行丙酮酸与D-赤藓糖-4-磷酸缩合形成3-脱氧-D-阿拉伯 - 庚酮酸7-磷酸（DAHP）; 使用3-脱氢秋水仙碱合酶将DAHP转化为3-脱氢秋水仙碱（DHQ）; DHQ脱水酶可以将DHQ转化为重要的莽草酸中间体，3-脱水莽草酸。