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公开(公告)号：US20090155893A1
公开(公告)日：2009-06-18
申请号：US12372904
申请日：2009-02-18
IPC分类号： C12M1/40
CPC分类号： G01N21/6428 , B01L7/52 , B01L99/00 , B01L2300/18 , B01L2400/0439 , C12Q1/6844 , G01N21/6408 , C12Q2563/107 , C12Q2561/113 , C12Q2523/301
摘要： Apparatus and methods for amplification of DNA are provided that use sonic energy in place of conventional thermocyclers. In one embodiment, sonic energy is applied to a PCR cocktail to effect dissociation of double stranded DNA into single strands of DNA. A quiescence stage, where no sonic energy is applied, results in amplification of DNA. Repetition of the cycles of application of sonic energy and cessation of application of sonic energy results in DNA amplification.
摘要翻译： 提供用于扩增DNA的装置和方法，其使用声能代替常规热循环仪。 在一个实施方案中，将声能量施加到PCR混合物以使双链DNA解离成单链DNA。 没有声波能量的静止阶段导致DNA的扩增。 重复使用声能的循环和停止应用声能导致DNA扩增。
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公开(公告)号：US07504219B2
公开(公告)日：2009-03-17
申请号：US11432935
申请日：2006-05-12
CPC分类号： G01N21/6428 , B01L7/52 , B01L99/00 , B01L2300/18 , B01L2400/0439 , C12Q1/6844 , G01N21/6408 , C12Q2563/107 , C12Q2561/113 , C12Q2523/301
摘要： Apparatus and methods for amplification of DNA are provided that use sonic energy in place of conventional thermocyclers. In one embodiment, sonic energy is applied to a PCR cocktail to effect dissociation of double stranded DNA into single strands of DNA. A quiescence stage, where no sonic energy is applied, results in amplification of DNA. Repetition of the cycles of application of sonic energy and cessation of application of sonic energy results in DNA amplification.
摘要翻译： 提供用于扩增DNA的装置和方法，其使用声能代替常规热循环仪。 在一个实施方案中，将声能量施加到PCR混合物以使双链DNA解离成单链DNA。 没有声波能量的静止阶段导致DNA的扩增。 重复使用声能的循环和停止应用声能导致DNA扩增。
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公开(公告)号：US20080280353A1
公开(公告)日：2008-11-13
申请号：US11902503
申请日：2007-09-21
IPC分类号： C12M1/00
CPC分类号： G01N33/542 , B82Y5/00 , B82Y10/00 , B82Y15/00 , C12Q1/68 , C12Q1/6834 , G01N33/58 , C12Q2563/155 , C12Q2563/137 , C12Q2523/301
摘要： A detection device comprised of a hybrid nucleic acid assembly. The device further comprises a nucleic acid polymer, a first nanoparticle conjugated to the nucleic acid polymer, a second nanoparticle conjugated with the nucleic acid polymer, a means for introducing energy into the first nanoparticle, means for detecting energy from the second nanoparticle, and means for determining a physical property of the nucleic acid polymer, wherein the means of introducing energy into the first nanoparticle is a source of mechanical energy.
摘要翻译： 一种由杂交核酸组合物组成的检测装置。 该装置还包括核酸聚合物，与核酸聚合物缀合的第一纳米颗粒，与核酸聚合物缀合的第二纳米颗粒，用于将能量引入第一纳米颗粒的装置，用于检测来自第二纳米颗粒的能量的装置，以及装置 用于确定核酸聚合物的物理性质，其中将能量引入第一纳米颗粒的手段是机械能的来源。
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公开(公告)号：US07294467B2
公开(公告)日：2007-11-13
申请号：US11138981
申请日：2005-05-26
申请人： Sang-Ryoul Park , In-Chul Yang , Myung-Sub Han , Yong-Hyeon Yim , Eui-Jin Hwang
发明人： Sang-Ryoul Park , In-Chul Yang , Myung-Sub Han , Yong-Hyeon Yim , Eui-Jin Hwang
CPC分类号： C12Q1/68 , C12Q2545/114 , C12Q2523/301 , C12Q2523/107
摘要： The present invention is related to a novel approach for quantitation of DNA based on the stoichiometric existence of phosphorus (P) in DNA.
摘要翻译： 本发明涉及基于DNA中磷（P）的化学计量存在的DNA定量的新方法。
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公开(公告)号：US20060024703A1
公开(公告)日：2006-02-02
申请号：US11142590
申请日：2005-06-01
申请人： Lixin Zhang , Betsy Foxman , Carl Marrs , Janet Gilsdorf , Usha Srinivasan , Debashis Ghosh
发明人： Lixin Zhang , Betsy Foxman , Carl Marrs , Janet Gilsdorf , Usha Srinivasan , Debashis Ghosh
CPC分类号： C12Q1/6837 , C12Q2565/513 , C12Q2523/301
摘要： The present invention relates to compositions and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences present in multiple genomes. In particular, the present invention provides microarrays possessing two or more whole genomes and methods of making and using the same to detect the presence or absence of target sequences in the plurality of genomes.
摘要翻译： 本发明涉及用于检测和表征核酸序列的组合物和方法以及存在于多个基因组中的核酸序列的变化。 特别地，本发明提供了具有两个或更多个全基因组的微阵列及其制备和使用它们以检测多个基因组中靶序列的存在或不存在的方法。
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公开(公告)号：US20040146918A1
公开(公告)日：2004-07-29
申请号：US10758875
申请日：2004-01-16
IPC分类号： C12Q001/68 , C12M001/34
CPC分类号： G01N33/542 , B82Y5/00 , B82Y10/00 , B82Y15/00 , C12Q1/68 , C12Q1/6834 , G01N33/58 , C12Q2563/155 , C12Q2563/137 , C12Q2523/301
摘要： A detection device comprised of a hybrid nucleic acid assembly. The device further comprises a nucleic acid polymer, a first nanoparticle conjugated to the nucleic acid polymer, a second nanoparticle conjugated with the nucleic acid polymer, a means for introducing energy into the first nanoparticle, means for detecting energy from the second nanoparticle, and means for determining a physical property of the nucleic acid polymer, wherein the means of introducing energy into the first nanoparticle is a source of mechanical energy.
摘要翻译： 一种由杂交核酸组合物组成的检测装置。 该装置还包括核酸聚合物，与核酸聚合物缀合的第一纳米颗粒，与核酸聚合物缀合的第二纳米颗粒，用于将能量引入第一纳米颗粒的装置，用于检测来自第二纳米颗粒的能量的装置，以及装置 用于确定核酸聚合物的物理性质，其中将能量引入第一纳米颗粒的手段是机械能的来源。
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57.发明授权公开(公告)号：US11725228B2
公开(公告)日：2023-08-15
申请号：US16754648
申请日：2018-10-11
IPC分类号： C12Q1/44 , C12Q1/6855 , C12Q1/6869
CPC分类号： C12Q1/44 , C12Q1/6855 , C12Q1/6869 , C12Q2521/301 , C12Q2523/301 , C12Q2525/191 , C12Q2531/113
摘要： Methodologies to detect off-target mutations induced by the deaminase activity of Base Editing technology.
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公开(公告)号：US10066260B2
公开(公告)日：2018-09-04
申请号：US14774662
申请日：2014-03-13
发明人： David Light , Wolfgang Hinz , Ronald L. Cicero , Christina E. Inman , Paul M. Kenney , Alexander Mastroianni , Roman Rozhkov , Yufang Wang , Jeremy Gray , Marc Glazer , Dmitriy Gremyachinskiy
IPC分类号： C12Q1/6837 , C12Q1/6806 , C12Q1/6869
CPC分类号： C12Q1/6837 , C12Q1/6806 , C12Q1/6834 , C12Q1/6869 , C12Q2523/101 , C12Q2527/119 , C12Q2535/122 , C12Q2547/107 , C12Q2563/159 , C12Q2565/537 , C12Q2523/301 , C12Q2523/32
摘要： A method of forming a polymer matrix array includes applying an aqueous solution into wells of a well array. The aqueous solution includes polymer precursors. The method further includes applying an immiscible fluid over the well array to isolate the aqueous solution within the wells of the well array and polymerizing the polymer precursors isolated in the wells of the well array to form the polymer matrix array. An apparatus includes a sensor array, a well array corresponding to the sensor array, and an array of polymer matrices disposed in the well array.
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公开(公告)号：US20180135120A1
公开(公告)日：2018-05-17
申请号：US15719722
申请日：2017-09-29
发明人： Yijun Ruan
IPC分类号： C12Q1/6869 , C12Q1/6806
CPC分类号： C12Q1/6869 , C12Q1/6806 , C12Q2521/501 , C12Q2523/303 , C12Q2525/191 , C12Q2535/122 , C12Q2523/301
摘要： The invention described herein provides methods and systems for comprehensive genomic analysis that enables the detection of a broad range of genomic variations, including single nucleotide polymorphisms (SNPs), small insertions or deletions (indels), Tandem Base Mutations (TBM), copy number variations (CNVs), structural variations (SVs), and combination thereof, in a single assay.The invention can be used, for example, to analyze the complicated underlying genomic defects in diseases and conditions such as Autism spectrum disorders (ASD), cancers, Alzheimer's disease, and other neurological disorders.
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公开(公告)号：US20180051341A1
公开(公告)日：2018-02-22
申请号：US15414260
申请日：2017-01-24
IPC分类号： C12Q1/68
CPC分类号： C12Y605/01001 , C12Q1/6806 , C12Y207/07007 , C12Y301/21002 , C12Y302/02023 , C12Q2521/514 , C12Q2523/301 , C12Q2535/122
摘要： Provided herein, among other things, is a method for reducing sequencing errors caused by mechanical DNA fragmentation. In some embodiments, this method may comprise: (a) obtaining a DNA sample; (b) mechanically fragmenting the DNA sample to produce a template; (c) treating the fragmented DNA with a plurality of DNA repair enzymes; and (d) obtaining a sequence of the fragmented DNA. The treatment step (c) reduces the number of fragmentation induced errors.
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