公开(公告)号：US10954309B2

公开(公告)日：2021-03-23

申请号：US15525688

申请日：2015-11-11

Applicant: Cytiva BioProcess R&D AB

Inventor： Gustav Rodrigo ,  Tomas Bjorkman ,  Mats Ander

IPC: C07K16/46 ,  B01J20/289 ,  B01J20/32 ,  B01D15/38 ,  C07K16/06 ,  C07K1/22 ,  C07K14/31 ,  C07K16/00

Abstract: An Fc-binding polypeptide of improved alkali stability, comprising a mutant of an Fc-binding domain of Staphylococcus Protein A (SpA), as defined by SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO:3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO 26 or SEQ ID NO 27, wherein at least the alanine residue at the position corresponding to position 42 in SEQ ID NO:4-7 has been mutated to arginine and/or wherein at least the aspartic acid residue at the position corresponding to position 37 in SEQ ID NO:4-7 has been mutated to glutamic acid.

公开(公告)号：US10928365B2

公开(公告)日：2021-02-23

申请号：US16459525

申请日：2019-07-01

Applicant: Cytiva BioProcess R&D AB

Inventor： Klaus Gebauer

IPC: G01N30/60 ,  B01D15/22 ,  G01N30/86 ,  G01N30/56 ,  B01D15/20 ,  B01D15/18

Abstract: The present invention relates to axial flow chromatography columns, methods for separating one or more analytes in a liquid by the use of such columns, and systems employing such columns. The column comprises a first port and a second port the first port and said second port being at essentially the same level or elevation above the level of the bed space on the chromatography column.

公开(公告)号：US10918971B2

公开(公告)日：2021-02-16

申请号：US15367640

申请日：2016-12-02

Applicant: Cytiva BioProcess R&D AB

Inventor： Sophia Hober

IPC: C07K1/22 ,  C07K14/195 ,  B01D15/38 ,  B01J20/286 ,  B01J20/32 ,  C07K16/06 ,  C07K14/31 ,  B01J20/24 ,  B01J20/289 ,  C07K14/00

Abstract: The present invention relates to an immunoglobulin-binding protein, wherein at least one asparagine residue has been mutated to an amino acid other than glutamine or aspartic acid, which mutation confers an increased chemical stability at pH-values of up to about 13-14 compared to the parental molecule. The protein can for example be derived from a protein capable of binding to other regions of the immunoglobulin molecule than the complementarity determining regions (CDR), such as protein A, and preferably the B-domain of Staphylococcal protein A. The invention also relates to a matrix for affinity separation, which comprises an immunoglobulin-binding protein as ligand coupled to a solid support, in which protein ligand at least one asparagine residue has been mutated to an amino acid other than glutamine.

公开(公告)号：US20210017223A1

公开(公告)日：2021-01-21

申请号：US16981716

申请日：2019-03-26

Applicant: Cytiva BioProcess R&D AB

Inventor： Tomas Bjorkman ,  Bengt Westerlund

IPC: C07K1/36 ,  C07K1/22 ,  C07K1/18 ,  C07K16/00

Abstract: The invention relates to a method of separating immunoglobulin variants, comprising the steps of: a) providing a column packed with an Fc-binding affinity chromatography resin; b) loading a sample comprising at least two Fc-comprising immunoglobulin variants onto the column; c) optionally washing the column with a washing liquid; and d) conveying an eluent through said column to elute at least a target immunoglobulin variant from said column and recovering one or more eluate fractions comprising the target immunoglobulin variant in enriched form.

公开(公告)号：US10894241B2

公开(公告)日：2021-01-19

申请号：US16306749

申请日：2017-06-22

Applicant: Cytiva BioProcess R&D AB

Inventor： Lin Chen ,  Jie Gao ,  Qiongqiong Huang

IPC: B01J13/04 ,  B05D7/24 ,  C08L5/12

Abstract: A method for forming polymer microparticles is disclosed. The method comprises spraying a liquid comprising a thermally-gelling polymer from a first orifice of a nozzle into air to form gelled microparticles, and collecting the gelled microparticles with a collecting device. The nozzle is positioned to have a central axis of the first orifice at a tilt angle in a range from about 10 degrees to about 170 degrees relative to a direction of gravity. The collecting device comprises at least two collecting units at different distances from the first orifice of the nozzle in a horizontal direction perpendicular to the direction of gravity. Each of the collecting unit is loaded with a liquid collecting medium. A system for forming polymer microparticles is also disclosed.

公开(公告)号：US12251681B2

公开(公告)日：2025-03-18

申请号：US17605502

申请日：2020-04-28

Applicant: Cytiva Bioprocess R&D AB

Inventor： Susanna Lindberg ,  Jonas Gustafsson ,  Linn Carlsson ,  Anders Hagvall ,  Erik Ringberg ,  David Jansson

IPC: B01J20/29 ,  B01J20/24 ,  B01J20/28 ,  B01J20/291 ,  B01J20/30

Abstract: The invention discloses method for manufacturing agar or agarose beads, comprising the steps of: a) providing a water phase comprising an aqueous solution of agar or agarose at a temperature of 40-100° C.: b) providing an oil phase comprising a water-immiscible solvent and an emulsifier at a temperature of 40-100° C.; c) emulsifying the water phase in the oil phase to form a water-in-oil emulsion: d) cooling the water-in-oil emulsion to a temperature below a gelation temperature of the agar or agarose to form a dispersion of solidified agar or agarose beads: and e) recovering agar or agarose beads from dispersion, wherein the emulsifier comprises a phosphate ester of an alkoxylated fatty alcohol.

公开(公告)号：US20240216831A1

公开(公告)日：2024-07-04

申请号：US18413586

申请日：2024-01-16

Applicant: Cytiva BioProcess R&D AB

Inventor： Klaus Gebauer

IPC: B01D15/14 ,  B01D15/18 ,  B01D15/22 ,  G01N30/46 ,  G01N30/60 ,  G01N30/88

CPC classification number: B01D15/14 ,  B01D15/1864 ,  B01D15/1885 ,  B01D15/22 ,  G01N30/466 ,  G01N30/6043 ,  G01N30/6091 ,  G01N30/6039 ,  G01N2030/8881 ,  Y10T29/49826

Abstract: A parallel assembly of chromatography column modules, the assembly having one common assembly inlet and one common assembly outlet, each column module comprising a bed space filled with chromatography medium and each column module comprises integrated fluid conduits which when the column module is connected with other column modules are adapted to connect the bed space of the column module with the assembly inlet and the assembly outlet, wherein the total length and/or volume of the fluid conduit from the assembly inlet to one bed space together with the length and/or volume of the fluid conduit from the same bed space to the assembly outlet is substantially the same for all bed spaces and modules installed in the parallel assembly.

公开(公告)号：US12005424B2

公开(公告)日：2024-06-11

申请号：US17045779

申请日：2019-04-24

Applicant: Cytiva BioProcess R&D AB

Inventor： Jonas Bengtsson ,  Linn Carlsson ,  Andreas Axen ,  Ronnie Palmgren

IPC: B01D15/08 ,  B01J20/24 ,  B01J20/26 ,  B01J20/28 ,  B01J20/285 ,  B01J20/291 ,  B01J20/30

CPC classification number: B01J20/267 ,  B01D15/08 ,  B01J20/24 ,  B01J20/28023 ,  B01J20/28047 ,  B01J20/285 ,  B01J20/291 ,  B01J20/3042 ,  B01J2220/445 ,  B01J2220/54

Abstract: The invention discloses a separation matrix comprising polysaccharide gel beads, wherein said polysaccharide gel beads comprise embedded fibers. The invention further discloses a method of preparing the separation matrix and use of the matrix for separation purposes.

公开(公告)号：US20240132538A1

公开(公告)日：2024-04-25

申请号：US17768461

申请日：2020-11-20

Applicant: Cytiva Bioprocess R&D AB

Inventor： Christopher James Sevinsky ,  Peter Lundback ,  Johan Ohman ,  Gregory Grossmann ,  Sean R. Dinn

IPC: C07K1/22 ,  C07K14/195 ,  C07K17/10

CPC classification number: C07K1/22 ,  C07K14/195 ,  C07K17/10 ,  C07K2319/92

Abstract: The present invention relates to protein purification, primarily in the chromatographic field. More closely, the invention relates to affinity chromatography using a split intein system with an improved C-intein tag and N-intein ligand, wherein the target protein may be purified as a tag-less end product with a native N-terminus.

公开(公告)号：US11820802B2

公开(公告)日：2023-11-21

申请号：US16328058

申请日：2017-09-05

Applicant: Cytiva BioProcess R&D AB

Inventor： Sophia Hober ,  Sara Kanje ,  Johan Nilvebrant

IPC: C07K14/47 ,  C07K1/22 ,  C07K14/31

CPC classification number: C07K14/4728 ,  C07K1/22 ,  C07K14/31 ,  C07K2319/20 ,  C07K2319/30 ,  C07K2319/705

Abstract: The present invention is within the field of protein engineering and purification. The invention relates to a target-binding polypeptide mutant of an IgG binding polypeptide, such as Protein A, Protein G, Protein L or Protein M, comprising a metal binding motif. More closely the invention relates to an Fc binding ligand comprising an engineered protein based on the Protein A derived Z domain, to which a calcium binding EF-loop has been introduced.