公开(公告)号：US10954309B2

公开(公告)日：2021-03-23

申请号：US15525688

申请日：2015-11-11

Applicant: Cytiva BioProcess R&D AB

Inventor： Gustav Rodrigo ,  Tomas Bjorkman ,  Mats Ander

IPC: C07K16/46 ,  B01J20/289 ,  B01J20/32 ,  B01D15/38 ,  C07K16/06 ,  C07K1/22 ,  C07K14/31 ,  C07K16/00

Abstract: An Fc-binding polypeptide of improved alkali stability, comprising a mutant of an Fc-binding domain of Staphylococcus Protein A (SpA), as defined by SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO:3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO 26 or SEQ ID NO 27, wherein at least the alanine residue at the position corresponding to position 42 in SEQ ID NO:4-7 has been mutated to arginine and/or wherein at least the aspartic acid residue at the position corresponding to position 37 in SEQ ID NO:4-7 has been mutated to glutamic acid.

公开(公告)号：US10928365B2

公开(公告)日：2021-02-23

申请号：US16459525

申请日：2019-07-01

Applicant: Cytiva BioProcess R&D AB

Inventor： Klaus Gebauer

IPC: G01N30/60 ,  B01D15/22 ,  G01N30/86 ,  G01N30/56 ,  B01D15/20 ,  B01D15/18

Abstract: The present invention relates to axial flow chromatography columns, methods for separating one or more analytes in a liquid by the use of such columns, and systems employing such columns. The column comprises a first port and a second port the first port and said second port being at essentially the same level or elevation above the level of the bed space on the chromatography column.

公开(公告)号：US10918971B2

公开(公告)日：2021-02-16

申请号：US15367640

申请日：2016-12-02

Applicant: Cytiva BioProcess R&D AB

Inventor： Sophia Hober

IPC: C07K1/22 ,  C07K14/195 ,  B01D15/38 ,  B01J20/286 ,  B01J20/32 ,  C07K16/06 ,  C07K14/31 ,  B01J20/24 ,  B01J20/289 ,  C07K14/00

Abstract: The present invention relates to an immunoglobulin-binding protein, wherein at least one asparagine residue has been mutated to an amino acid other than glutamine or aspartic acid, which mutation confers an increased chemical stability at pH-values of up to about 13-14 compared to the parental molecule. The protein can for example be derived from a protein capable of binding to other regions of the immunoglobulin molecule than the complementarity determining regions (CDR), such as protein A, and preferably the B-domain of Staphylococcal protein A. The invention also relates to a matrix for affinity separation, which comprises an immunoglobulin-binding protein as ligand coupled to a solid support, in which protein ligand at least one asparagine residue has been mutated to an amino acid other than glutamine.

公开(公告)号：US20210017223A1

公开(公告)日：2021-01-21

申请号：US16981716

申请日：2019-03-26

Applicant: Cytiva BioProcess R&D AB

Inventor： Tomas Bjorkman ,  Bengt Westerlund

IPC: C07K1/36 ,  C07K1/22 ,  C07K1/18 ,  C07K16/00

Abstract: The invention relates to a method of separating immunoglobulin variants, comprising the steps of: a) providing a column packed with an Fc-binding affinity chromatography resin; b) loading a sample comprising at least two Fc-comprising immunoglobulin variants onto the column; c) optionally washing the column with a washing liquid; and d) conveying an eluent through said column to elute at least a target immunoglobulin variant from said column and recovering one or more eluate fractions comprising the target immunoglobulin variant in enriched form.

公开(公告)号：US10894241B2

公开(公告)日：2021-01-19

申请号：US16306749

申请日：2017-06-22

Applicant: Cytiva BioProcess R&D AB

Inventor： Lin Chen ,  Jie Gao ,  Qiongqiong Huang

IPC: B01J13/04 ,  B05D7/24 ,  C08L5/12

Abstract: A method for forming polymer microparticles is disclosed. The method comprises spraying a liquid comprising a thermally-gelling polymer from a first orifice of a nozzle into air to form gelled microparticles, and collecting the gelled microparticles with a collecting device. The nozzle is positioned to have a central axis of the first orifice at a tilt angle in a range from about 10 degrees to about 170 degrees relative to a direction of gravity. The collecting device comprises at least two collecting units at different distances from the first orifice of the nozzle in a horizontal direction perpendicular to the direction of gravity. Each of the collecting unit is loaded with a liquid collecting medium. A system for forming polymer microparticles is also disclosed.

公开(公告)号：US20250153140A1

公开(公告)日：2025-05-15

申请号：US18839374

申请日：2023-02-16

Applicant: Cytiva BioProcess R&D AB

Inventor： Jean-Luc Maloisel ,  Frederic Leroux ,  Lisa Marx

IPC: B01J20/289 ,  B01D15/36 ,  B01D15/38 ,  B01J20/24 ,  B01J20/26 ,  B01J20/28 ,  B01J20/32

Abstract: A chromatography medium is provided, comprising a matrix of cellulose-based nanofibers, the nanofibers optionally being crosslinked to one another. A ligand coupled to the matrix without any intermediate extender group. Also provided is a method of preparing a functionalised chromatography medium. The method comprises: (i) providing a substrate comprising cellulose acetate; (ii) forming a fibrous matrix/membrane spun of nanofibers from the substrate; (iii) saponification of the nanofibers to form regenerated cellulose nanofibers; (iv) derivatisation of the regenerated cellulose nanofibers with a cross-linker, and (v) coupling of a ligand to the derivatised cellulose nanofibers, wherein the preparation of the functionalised chromatography medium does not comprise any surface extender. The chromatography medium is useful for separation of large analytes, such as viruses.

公开(公告)号：US20250058298A1

公开(公告)日：2025-02-20

申请号：US18712580

申请日：2022-12-06

Applicant: Cytiva BioProcess R&D AB

Inventor： Jean-Luc Maloisel ,  Åsa Hagner McWhirter ,  Ola Lind ,  Ida Helena Vestin ,  Erik Tingborn ,  Björn Norén

IPC: B01J20/28 ,  B01J20/24 ,  B01J20/282 ,  B01J20/32

Abstract: The present disclosure is directed to a separation matrix comprising a plurality of chromatography particles, each chromatography particle comprising a core and a layer surrounding the core, wherein the core has a first average pore diameter and the layer surrounding the core has a second average pore diameter, wherein the second average pore diameter is at least 1.5 times higher than the first average pore diameter, wherein the first average pore diameter excludes diffusion of a target molecule through the pores of the core and wherein the second average pore diameter at least partly permits diffusion of the target molecule through the pores of the layer surrounding the core. Further disclosed are a method for preparing such a separation matrix, uses of such a separation matrix and methods for separating target molecules by use of such a separation matrix, in particular a method for separating adeno associated virus capsids fully packaged with genetic material from adeno associated virus capsids not fully packaged with genetic material, and compositions obtained by said method.

公开(公告)号：US20240261705A1

公开(公告)日：2024-08-08

申请号：US18566551

申请日：2022-06-27

Applicant: Cytiva BioProcess R&D AB

Inventor： Jean-Luc Maloisel ,  Asa Hagner McWhirter ,  Ola Lind ,  Frederic Leroux

IPC: B01D15/36 ,  B01J41/20 ,  C12N7/02

CPC classification number: B01D15/363 ,  B01J41/20 ,  C12N7/02

Abstract: An anion exchange chromatography medium (1) for use in purification of enveloped virus particles or exosomes from a feed, the anion exchange chromatography medium comprising a support material being functionalized with a ligand comprising a diamine functionality generating at least one weak anion exchange group to an ionic capacity of 10-500 μmol/mL.

公开(公告)号：US20240226357A1

公开(公告)日：2024-07-11

申请号：US18604231

申请日：2024-03-13

Applicant: Cytiva BioProcess R&D AB

Inventor： Elin Marianne Monie ,  Tomas Bjorkman ,  Anna Gronberg ,  Anders Ljunglof ,  Gustav Jose Rodrigo ,  Karin Torstenson ,  Magnus Carl Erik Wetterhall

IPC: A61L2/18 ,  B01D15/20 ,  B01D15/38 ,  G01N30/50

CPC classification number: A61L2/18 ,  B01D15/203 ,  B01D15/3804 ,  G01N30/50

Abstract: The invention discloses a method for cleaning or sanitization of an affinity chromatography matrix, comprising the steps of:
a) providing an affinity chromatography matrix having oxidation-tolerant proteinaceous ligands coupled to a support,
b) contacting the matrix with a sanitization solution comprising at least one oxidant defined by formula I,




wherein R is hydrogen or an acyl group R′—C(O)—, with R′ being a hydrogen or a methyl, ethyl or propyl group.

公开(公告)号：US20240076650A1

公开(公告)日：2024-03-07

申请号：US18260560

申请日：2022-01-26

Applicant: CYTIVA BIOPROCESS R&D AB

Inventor： Peter Lundback ,  Ronnie Palmgren ,  Ola Lind ,  Nils Norrman ,  Helen Cheek ,  Susana Brito Dos Santos ,  Per Denker

IPC: C12N15/10 ,  B01D15/38 ,  B01J20/26 ,  B01J20/28 ,  B01J20/289

CPC classification number: C12N15/101 ,  B01D15/3819 ,  B01J20/265 ,  B01J20/28035 ,  B01J20/28038 ,  B01J20/289 ,  B01J2220/52

Abstract: The invention relates to processes for purification of poly-tagged products, such as mRNA, from synthetic or biological compositions. The process involves contacting the composition with a oligo d (T)-functionalized chromatography medium comprising a convection-based chromatography material.