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公开(公告)号：US20180243665A1
公开(公告)日：2018-08-30
申请号：US15753341
申请日：2016-08-18
Applicant: GE HEALTHCARE BIOPROCESS R&D AB
Inventor： Karol Maciej Lacki , Hans Blom , Helena Skoglar , Linus Laurin
CPC classification number: B01D15/3809 , B01J20/24 , B01J20/28004 , B01J20/28011 , B01J20/28019 , B01J20/286 , B01J20/3212 , B01J20/3274 , B01J20/3278 , B01J20/3293 , B01J2220/603 , C07K1/22 , C07K16/065
Abstract: The invention discloses a separation matrix comprised of porous spherical particles to which antibody-binding protein ligands have been covalently immobilized, wherein the density of said ligands is in the range of 10.5-15 mg/ml and the volume-weighted median diameter of said particles is in the range of 30-55 μm. The invention further discloses a method of separation of antibodies by affinity chromatography which employs the said separation matrix within a chromatography column.
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公开(公告)号：US20180094024A1
公开(公告)日：2018-04-05
申请号：US15348699
申请日：2016-11-10
Applicant: GE Healthcare BioProcess R&D AB
Inventor： Annika Kristina FORSS , Gustav Jose RODRIGO , Tomas BJORKMAN , Jesper Ulf Hansson , Mats Ander
IPC: C07K1/22 , C07K16/12 , C07K17/10 , B01J20/286
Abstract: The invention relates to a method of isolating an immunoglobulin, comprising the steps of:a) providing a separation matrix comprising multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support, b) contacting a liquid sample comprising an immunoglobulin with the separation matrix, c) washing said separation matrix with a washing liquid, d) eluting the immunoglobulin from the separation matrix with an elution liquid, and e) cleaning the separation matrix with a cleaning liquid, wherein the alkali-stabilized Protein A domains comprise mutants of a parental Fc-binding domain of Staphylococcus Protein A (SpA), as defined by, or having at least 80% such as at least 90%, 95% or 98% identity to, SEQ ID NO 51 or SEQ ID NO 52, wherein the amino acid residues at positions 13 and 44 of SEQ ID NO 51 or 52 are asparagines and wherein at least the asparagine residue at position 3 of SEQ ID NO 51 or 52 has been mutated to an amino acid selected from the group consisting of glutamic acid, lysine, tyrosine, threonine, phenylalanine, leucine, isoleucine, tryptophan, methionine, valine, alanine, histidine and arginine.
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公开(公告)号：US20170334954A1
公开(公告)日：2017-11-23
申请号：US15526941
申请日：2015-11-16
Applicant: GE HEALTHCARE BIOPROCESS R&D AB
Inventor： Gustav Rodrigo , Tomas Bjorkman , Mats Ander
CPC classification number: C07K16/46 , B01D15/3809 , B01J20/289 , B01J20/3219 , B01J20/3274 , C07K1/22 , C07K14/31 , C07K16/00 , C07K16/065
Abstract: An Fc-binding polypeptide of improved alkali stability, comprising a mutant of an Fc-binding domain of Staphylococcus Protein A (SpA), as defined by SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO:3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:22, SEQ ID NO 51 or SEQ ID NO 52 wherein at least the asparagine or serine residue at the position corresponding to position 11 in SEQ ID NO:4-7 has been mutated to an amino acid selected from the group consisting of glutamic acid, lysine, tyrosine, threonine, phenylalanine, leucine, isoleucine, tryptophan, methionine, valine, alanine, histidine and arginine.
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公开(公告)号：US20170247407A1
公开(公告)日：2017-08-31
申请号：US15596040
申请日：2017-05-16
Applicant: GE Healthcare BioProcess R&D AB
Inventor： Hans J. Johansson , Ronnie Palmgren
IPC: C07K1/22 , B01D15/38 , B01J20/285 , C07K14/31 , B01J20/289
CPC classification number: C07K1/22 , B01D15/34 , B01D15/3809 , B01J20/285 , B01J20/286 , B01J20/289 , B01J20/3274 , C07K14/245 , C07K14/31 , C07K2319/00
Abstract: The invention discloses a polypeptide capable of binding immunoglobulins or immunoglobulin-containing proteins, which polypeptide comprises six or more domains of protein Z or the C domain of protein A or a functional variant thereof. It also discloses separation matrices comprising the polypeptide and methods of using the separation matrices for separation of immunoglobulins or immunoglobulin-containing proteins.
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公开(公告)号：US20170182432A1
公开(公告)日：2017-06-29
申请号：US15458667
申请日：2017-03-14
Applicant: GE Healthcare BioProcess R&D AB
Inventor： Klaus Gebauer
CPC classification number: B01D15/206 , B01D15/14 , B01D15/22 , G01N30/56 , G01N30/6021 , G01N30/603 , G01N30/606 , G01N2030/565
Abstract: The invention relates to a method for providing an aseptic chromatography column, said method comprising the steps of: pre-sterilize an empty chromatography column; pre-sterilize a chromatography medium; introducing the pre-sterilized chromatography medium into the pre-sterilized chromatography column using aseptic equipment, thereby providing an aseptic chromatography column comprising chromatography medium.
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Patent Agency Ranking
公开(公告)号：US09597611B2
公开(公告)日：2017-03-21
申请号：US13672832
申请日：2012-11-09
Applicant: GE HEALTHCARE BIOPROCESS R&D AB
Inventor： Klaus Gebauer
CPC classification number: B01D15/206 , B01D15/14 , B01D15/22 , G01N30/56 , G01N30/6021 , G01N30/603 , G01N30/606 , G01N2030/565
Abstract: A method for providing an aseptic chromatography column includes the steps of pre-sterilizing an empty chromatography column, pre-sterilizing a chromatography medium, introducing the pre-sterilized chromatography medium into the pre-sterilized chromatography column using aseptic equipment, thus providing an aseptic chromatography column comprising chromatography medium.
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公开(公告)号：US20170051010A1
公开(公告)日：2017-02-23
申请号：US15308327
申请日：2015-06-04
Applicant: GE Healthcare BioProcess R&D AB
Inventor： Jean-Luc Maloisel , Ola Lind , Bjorn Noren , Ronnie Palmgren
CPC classification number: C07K1/22 , C07K1/145 , C07K16/00 , C07K16/065 , C07K2317/55
Abstract: The present invention relates to a method for removal of large contaminants, such as virus, in a chromatographic process for purification of a target molecule, preferably monoclonal antibodies, mAbs, by using a specifically designed chromatographic bead having a thin outer layer and a core functionalized with a ligand adsorbing the mAbs or parts thereof.
Abstract translation: 本发明涉及在用于纯化目标分子的色谱方法（优选单克隆抗体mAb）中通过使用具有薄的外层和核心功能化的专门设计的色谱珠来去除大污染物如病毒的方法 具有吸附mAb或其部分的配体。
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公开(公告)号：US20170050121A1
公开(公告)日：2017-02-23
申请号：US15183526
申请日：2016-06-15
Applicant: GE Healthcare BioProcess R&D AB
Inventor： JAN BERGSTROM , GUNNAR GLAD , BO-LENNART JOHANSSON , JEAN-LUC MALOISEL , NILS NORRMAN , TOBIAS E. SODERMAN
IPC: B01D15/32 , B01J20/287 , C12N7/00 , G01N1/40 , C07K1/20 , C07K16/00 , B01J20/285 , B01J39/26
CPC classification number: B01D15/327 , B01J20/28009 , B01J20/285 , B01J20/287 , B01J20/3212 , B01J20/3293 , B01J39/26 , C07K1/20 , C07K16/00 , C12N7/00 , C12N2760/16151 , G01N1/405
Abstract: The present invention is within the field of chromatography. More precisely, it relates to a novel chromatography medium, namely a hydrophobic medium provided with different lids excluding molecules over a certain size due to the porosity of the hydrophobic medium and/or the porosity of the lid. The invention also relates to use of the separation medium for purification of large molecules, which do not enter the separation medium, as well as small molecules, which enter the separation medium and are eluted from there.
Abstract translation: 本发明在色谱领域内。 更准确地说，它涉及一种新颖的色谱介质，即由于疏水介质的孔隙率和/或盖的孔隙度，提供了不同盖子的疏水介质，其中排除了一定尺寸的分子。 本发明还涉及用于纯化不分离介质的大分子的分离介质以及进入分离介质并从其中洗脱的小分子的用途。
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公开(公告)号：US20160243526A1
公开(公告)日：2016-08-25
申请号：US15027146
申请日：2014-10-09
Applicant: GE HEALTHCARE BIOPROCESS R&D AB
Inventor： Tobias E. Soderman
IPC: B01J20/32 , C08F290/10 , B01D15/32 , B01J20/287
Abstract: The present invention relates to a method for production of a chromatography material. More closely, the invention relates to a method for production of a reverse phase chromatography (RPC) material comprising the following steps: introduction of unsaturated groups onto porous carbohydrate particles and grafting of styrenic monomers on said particles comprising an unsaturated group.
Abstract translation: 本发明涉及色谱材料的制造方法。 更详细地说，本发明涉及一种生产反相色谱（RPC）材料的方法，包括以下步骤：将不饱和基团引入到多孔碳水化合物颗粒上，并将苯乙烯单体接枝到包含不饱和基团的所述颗粒上。
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公开(公告)号：US10933350B2
公开(公告)日：2021-03-02
申请号：US16154639
申请日：2018-10-08
Applicant: GE Healthcare BioProcess R&D AB
Inventor： Klaus Gebauer
Abstract: A parallel assembly of chromatography column modules, the assembly having one common assembly inlet and one common assembly outlet, each column module comprising a bed space filled with chromatography medium and each column module comprises integrated fluid conduits which when the column module is connected with other column modules are adapted to connect the bed space of the column module with the assembly inlet and the assembly outlet, wherein the total length and/or volume of the fluid conduit from the assembly inlet to one bed space together with the length and/or volume of the fluid conduit from the same bed space to the assembly outlet is substantially the same for all bed spaces and modules installed in the parallel assembly.
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