公开(公告)号：US5578444A

公开(公告)日：1996-11-26

申请号：US171389

申请日：1993-12-20

Applicant: Cynthia A. Edwards ,  Charles R. Cantor ,  Beth M. Andrews ,  Lisa M. Turin ,  Kirk E. Fry

Inventor： Cynthia A. Edwards ,  Charles R. Cantor ,  Beth M. Andrews ,  Lisa M. Turin ,  Kirk E. Fry

IPC: C07K14/47 ,  C12N15/10 ,  C12N15/67 ,  C12Q1/68 ,  C12Q1/70 ,  C07H21/02 ,  C12N15/00 ,  G01N33/574

CPC classification number: C12Q1/701 ,  C07K14/47 ,  C12N15/1048 ,  C12N15/67 ,  C12Q1/6811 ,  C12Q1/6813 ,  C12Q1/6876 ,  C12Q1/6883 ,  C12Q1/705 ,  H01L2924/14

Abstract: The present invention defines a DNA:protein-binding assay useful for screening libraries of synthetic or biological compounds for their ability to bind DNA test sequences. The assay is versatile in that any number of test sequences can be tested by placing the test sequence adjacent to a defined protein binding screening sequence. Binding of molecules to these test sequence changes the binding characteristics of the protein molecule to its cognate binding sequence. When such a molecule binds the test sequence the equilibrium of the DNA:protein complexes is disturbed, generating changes in the concentration of free DNA probe. Numerous exemplary target test sequences (SEQ ID NO:1 to SEQ ID NO:600) are set forth. The assay of the present invention is also useful to characterize the preferred binding sequences of any selected DNA-binding molecule.

Abstract translation: 本发明定义了一种DNA：蛋白结合测定法，用于筛选合成或生物化合物文库结合DNA测试序列的能力。 该测定法是通用的，因为可以通过将测试序列置于与定义的蛋白质结合筛选序列相邻的位置来测试任何数量的测试序列。 分子与这些测试序列的结合改变了蛋白质分子与其同源结合序列的结合特征。 当这样的分子结合测试序列时，DNA：蛋白复合物的平衡受到干扰，产生游离DNA探针浓度的变化。 阐述了许多示例性目标测试序列（SEQ ID NO：1至SEQ ID NO：600）。 本发明的测定也可用于表征任何所选DNA结合分子的优选结合序列。

公开(公告)号：US5328985A

公开(公告)日：1994-07-12

申请号：US729460

申请日：1991-07-12

Applicant: Takeshi Sano ,  Charles R. Cantor

Inventor： Takeshi Sano ,  Charles R. Cantor

IPC: A61K38/00 ,  A61K47/48 ,  C07K14/31 ,  C07K14/36 ,  C12Q1/68 ,  C07K3/00 ,  A61K35/14 ,  C07H21/04 ,  C12Q1/00

CPC classification number: B82Y5/00 ,  A61K47/48753 ,  C07K14/31 ,  C07K14/36 ,  A61K38/00

Abstract: A novel recombinant streptavidin-protein A chimeric protein which allows conjugation of antibody molecules with biological materials. The chimeric protein is efficiently expressed in Escherichia coli and is purified by simple procedures. The purified chimetic protein can bind one biotin molecule and one to two immunoglobulin molecules per subunit.

Abstract translation: 一种新的重组链霉亲和素蛋白A嵌合蛋白，其允许抗体分子与生物材料缀合。 嵌合蛋白在大肠杆菌中有效表达，并通过简单的方法纯化。 纯化的模仿蛋白可以结合一个生物素分子和每个亚基一至两个免疫球蛋白分子。

公开(公告)号：US4868311A

公开(公告)日：1989-09-19

申请号：US847422

申请日：1988-04-02

Applicant: Wilma A. Saffran ,  Richard L. Edelson ,  Francis P. Gasparro ,  John T. Welsh ,  Charles R. Cantor

Inventor： Wilma A. Saffran ,  Richard L. Edelson ,  Francis P. Gasparro ,  John T. Welsh ,  Charles R. Cantor

IPC: C07D519/00

CPC classification number: C07D519/00 ,  Y10T436/141111

Abstract: The present invention provides a compound having the formula ##STR1## wherein Y is biotin or iminobiotin, X is CH.sub.2, P is psoralen or a psoralen derivative, r is an integer equal to or greater than 2 and s is an integer equal to or greater than 1.The invention also provides a method for preparing a biotinylated psoralen or a biotinylated psoralen derivative.Further provided are methods for detecting, purifying, and isolating nucleic acids, and methods for delivering an iminobiotinylated psoralen to a cell.

Abstract translation: 本发明提供了具有式“IMAGE”的化合物，其中Y是生物素或亚氨基生物素，X是CH 2，P是补骨脂素或补骨脂素衍生物，r是等于或大于2的整数，s是等于或大于 本发明还提供了制备生物素化补骨脂素或生物素化补骨脂素衍生物的方法。 进一步提供了用于检测，纯化和分离核酸的方法，以及将亚氨基生物碱化补骨脂素递送至细胞的方法。

公开(公告)号：US4861448A

公开(公告)日：1989-08-29

申请号：US99535

申请日：1987-09-22

Applicant: Charles R. Cantor ,  David C. Schwartz

Inventor： Charles R. Cantor ,  David C. Schwartz

IPC: G01N27/447

CPC classification number: G01N27/44743 ,  G01N27/44773

Abstract: Gel inserts comprising a solidified liquid such as agarose suitable for use in an electrophoretic method, lysed cells entrapped within a matrix formed by the solidified liquid and macromolecules such as DNA or intact chromosomes derived from the lysed cells may be advantageously used in electrophoretic separations. The gel inserts are placed directly in a suitable support medium and subjected to one or more electric fields to separate the macromolecules.

Abstract translation: 包含适合用于电泳方法的固化液体如琼脂糖的凝胶插入物，包埋在由固化液体形成的基质中的裂解细胞和大分子如DNA或来自裂解细胞的完整染色体可以有利地用于电泳分离。 将凝胶插入物直接放置在合适的载体介质中并经受一个或多个电场以分离大分子。

公开(公告)号：US4695548A

公开(公告)日：1987-09-22

申请号：US654641

申请日：1984-09-25

Applicant: Charles R. Cantor ,  David C. Schwartz

Inventor： Charles R. Cantor ,  David C. Schwartz

IPC: G01N27/447 ,  C12N11/12

CPC classification number: G01N27/44773 ,  Y10T428/2984 ,  Y10T428/2987

Abstract: Gel inserts comprising a solidified liquid such as agarose suitable for use in an electrophoretic method, lysed cells entrapped within a matrix formed by the solidified liquid and macromolecules such as DNA or intact chromosomes derived from the lysed cells may be advantageously used in electrophoretic separations. The gel inserts are placed directly in a suitable support medium and subjected to one or more electric fields to separate the macromolecules.

Abstract translation: 包含适合用于电泳方法的固化液体如琼脂糖的凝胶插入物，包埋在由固化液体形成的基质中的裂解细胞和大分子如DNA或来自裂解细胞的完整染色体可以有利地用于电泳分离。 将凝胶插入物直接放置在合适的载体介质中并经受一个或多个电场以分离大分子。

公开(公告)号：US09534224B2

公开(公告)日：2017-01-03

申请号：US10535128

申请日：2003-11-14

Applicant: James J. Collins ,  Farren J. Isaacs ,  Charles R. Cantor ,  Daniel J. Dwyer

Inventor： James J. Collins ,  Farren J. Isaacs ,  Charles R. Cantor ,  Daniel J. Dwyer

IPC: C12N15/67 ,  C12N15/11 ,  C12Q1/68

CPC classification number: C12N15/67 ,  C12N15/11 ,  C12N2310/53 ,  C12Q1/6897

Abstract: The present invention provides nucleic acid molecules, DNA constructs, plasmids, and methods for post-transcriptional regulation of gene expression using RNA molecules to both repress and activate translation of an open reading frame. Repression of gene expression is achieved through the presence of a regulatory nucleic acid element (the cis-repressive RNA or crRNA) within the 5′ untranslated region (5′ UTR) of an mRNA molecule. The nucleic acid element forms a hairpin (stem/loop) structure through complementary base pairing. The hairpin blocks access to the mRNA transcript by the ribosome, thereby preventing translation. In particular, in embodiments of the invention designed to operate in prokaryotic cells, the stem of the hairpin secondary structure sequesters the ribosome binding site (RBS). In embodiments of the invention designed to operate in eukaryotic cells, the stem of the hairpin is positioned upstream of the start codon, anywhere within the 5′ UTR of an mRNA. A small RNA (trans-activating RNA, or taRNA), expressed in trans, interacts with the crRNA and alters the hairpin structure. This alteration allows the ribosome to gain access to the region of the transcript upstream of the start codon, thereby activating transcription from its previously repressed state.

Abstract translation: 本发明提供核酸分子，DNA构建体，质粒和用于转录后调节基因表达的方法，其使用RNA分子抑制和激活开放阅读框的翻译。 通过在mRNA分子的5'非翻译区（5'UTR）内存在调节性核酸元件（顺式抑制性RNA或crRNA）来实现基因表达的抑制。 核酸元件通过互补碱基配对形成发夹（茎/环）结构。 发夹阻止核糖体进入mRNA转录物，从而阻止翻译。 特别地，在设计用于在原核细胞中操作的本发明的实施方案中，发夹二级结构的茎螯合核糖体结合位点（RBS）。 在设计用于在真核细胞中操作的本发明的实施方案中，发夹的茎位于起始密码子的上游，位于mRNA的5'UTR内的任何位置。 以反式表达的小RNA（反式激活RNA或taRNA）与crRNA相互作用并改变发夹结构。 这种改变允许核糖体进入起始密码子上游的转录物区域，从而从其先前的抑制状态激活转录。

公开(公告)号：US09034580B2

公开(公告)日：2015-05-19

申请号：US12354749

申请日：2009-01-15

Applicant: Charles R. Cantor

Inventor： Charles R. Cantor

IPC: C12Q1/68 ,  C12P19/34

CPC classification number: C12Q1/6876 ,  C12Q1/6806 ,  C12Q1/6837 ,  C12Q1/6844 ,  C12Q2565/537 ,  C12Q2565/519 ,  C12Q2565/501 ,  C12Q2531/101 ,  C12Q2525/161 ,  C12Q2525/155

Abstract: Improved solid supports and methods for analyzing target nucleotide sequences are provided herein. Certain improvements are directed to efficiently preparing nucleic acids that comprise nucleotide sequences identical to or substantially identical to one or more target nucleotide sequences, or complement thereof. The prepared nucleic acids include a reference sequence that facilitates sequence analysis. The solid supports and methods provided herein minimize the number of steps required by published sequence analysis methodologies, and thereby offer improved sequence analysis efficiency.

Abstract translation: 本文提供了改进的固体支持物和分析靶核苷酸序列的方法。 某些改进旨在有效地制备包含与一个或多个靶核苷酸序列或其互补序列相同或基本相同的核苷酸序列的核酸。 所制备的核酸包括促进序列分析的参考序列。 本文提供的固体支持物和方法使发布的序列分析方法所需的步骤数量最小化，从而提供改进的序列分析效率。

公开(公告)号：US20110263453A1

公开(公告)日：2011-10-27

申请号：US13129797

申请日：2009-11-20

Applicant: Charles R. Cantor

Inventor： Charles R. Cantor

IPC: C40B30/04 ,  C12Q1/68 ,  G01N33/53 ,  B82Y15/00

Abstract: Described herein are products and processes for nucleic acid quantification, which are in part useful for detecting and determining the nucleotide sequence of rare nucleic acids (i.e., low copy number nucleic acids) in a sample. Such products and processes are useful for reducing the dynamic range among different nucleic acid species.

Abstract translation: 本文描述的是用于核酸定量的产物和方法，其部分可用于检测和测定样品中稀有核酸（即低拷贝数核酸）的核苷酸序列。 这样的产物和方法可用于减少不同核酸物种之间的动态范围。

公开(公告)号：US20090220942A1

公开(公告)日：2009-09-03

申请号：US12091709

申请日：2006-10-27

Applicant: Natalia Broude ,  Charles R. Cantor ,  Vadim V. Demidov

Inventor： Natalia Broude ,  Charles R. Cantor ,  Vadim V. Demidov

IPC: C12Q1/70 ,  C12Q1/68 ,  C07K14/00 ,  C12P21/00

CPC classification number: C12Q1/6883

Abstract: The present invention relates to a method to produce activated split-polypeptide fragments that on reconstitution immediately forms an active protein. The method relate to real-time protein complementation. Also encompassed in the invention is a method to split and produce split-fluorescent proteins in an active state which produce a fluorescent signal immediately on reconstitution. The present application also provides methods to detect nucleic acids; non-nucleic acid analytes and nucleic acid hybridization in real-time using the novel activated split-polypeptide fragments of the invention.

Abstract translation: 本发明涉及一种产生活化的裂多肽片段的方法，其在重构时立即形成活性蛋白质。 该方法涉及实时蛋白质互补。 本发明还包括分裂和产生活性状态的分裂荧光蛋白的方法，其在重建时立即产生荧光信号。 本申请还提供了检测核酸的方法; 非核酸分析物和核酸杂交，使用本发明的新型活化的裂解多肽片段。

公开(公告)号：US20090202984A1

公开(公告)日：2009-08-13

申请号：US12354749

申请日：2009-01-15

Applicant: Charles R. Cantor

Inventor： Charles R. Cantor

IPC: C12Q1/70 ,  C12Q1/68

CPC classification number: C12Q1/6876 ,  C12Q1/6806 ,  C12Q1/6837 ,  C12Q1/6844 ,  C12Q2565/537 ,  C12Q2565/519 ,  C12Q2565/501 ,  C12Q2531/101 ,  C12Q2525/161 ,  C12Q2525/155

Abstract: Improved solid supports and methods for analyzing target nucleotide sequences are provided herein. Certain improvements are directed to efficiently preparing nucleic acids that comprise nucleotide sequences identical to or substantially identical to one or more target nucleotide sequences, or complement thereof. The prepared nucleic acids include a reference sequence that facilitates sequence analysis. The solid supports and methods provided herein minimize the number of steps required by published sequence analysis methodologies, and thereby offer improved sequence analysis efficiency.

Abstract translation: 本文提供了改进的固体支持物和分析靶核苷酸序列的方法。 某些改进旨在有效地制备包含与一个或多个靶核苷酸序列或其互补序列相同或基本相同的核苷酸序列的核酸。 所制备的核酸包括促进序列分析的参考序列。 本文提供的固体支持物和方法使发布的序列分析方法所需的步骤数量最小化，从而提供改进的序列分析效率。