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    PROPIONIBACTERIUM ACNES AND GRANULOSUM PARIETAL FRACTION HAVING IMMUNOMODULATING ACTION
    62.
    发明申请
    PROPIONIBACTERIUM ACNES AND GRANULOSUM PARIETAL FRACTION HAVING IMMUNOMODULATING ACTION 审中-公开

    公开(公告)号：US20170202886A1

    公开(公告)日：2017-07-20

    申请号：US15326265

    申请日：2015-07-16

    申请人： DEPOFARMA S.P.A.

    发明人： Paola DALLA ZORZA ,  Alessandra DALLA ZORZA

    IPC分类号： A61K35/74 ,  C12N1/06 ,  C12N1/20

    摘要： The present invention relates to a free parietal fraction of Propionibacterium acnes and granulosum, obtained by delipidation and controlled crushing of the strain ATCC51277 or DSM20458. Said free fraction is particularly useful as an immunomodulating agent for a series of pathologies, being further characterized in that it has a high inhibitory effect against the symptoms associated with disorders. The fraction obtained can moreover be formulated in pharmaceutical compositions for local topical use, for example in gels.

    Protein extraction methods
    64.
    发明授权
    Protein extraction methods 有权
    蛋白质提取方法

    公开(公告)号：US09580462B2

    公开(公告)日：2017-02-28

    申请号：US13223737

    申请日：2011-09-01

    申请人： Lin Yan ,  Quanzhi Li

    发明人： Lin Yan ,  Quanzhi Li

    IPC分类号： C07K1/34 ,  C12N1/06 ,  C07K1/00

    CPC分类号： C07K1/34

    摘要： The present invention relates to methods for isolating polypepitdes from cells. The methods may be used to isolate denatured polypeptides or native polypeptides. In one embodiment the methods may be used to isolated polypeptide from cytosolic and nuclear compartments of eukaryotic cells. In one embodiment the methods may be used to isolate polypeptides from bacterial cells.

    摘要翻译： 本发明涉及从细胞中分离多肽的方法。 该方法可用于分离变性多肽或天然多肽。 在一个实施方案中，该方法可用于来自真核细胞的胞质和核隔室的分离的多肽。 在一个实施方案中，所述方法可用于从细菌细胞中分离多肽。

    OPTIMISED METHOD FOR BREAKING CHLORELLA WALLS BY MECHANICAL CRUSHING
    66.
    发明申请
    OPTIMISED METHOD FOR BREAKING CHLORELLA WALLS BY MECHANICAL CRUSHING 审中-公开
    通过机械破碎破坏氯氰酸盐的优化方法

    公开(公告)号：US20160376542A1

    公开(公告)日：2016-12-29

    申请号：US14902430

    申请日：2014-07-03

    申请人： ROQUETTE FRERES

    发明人： Beatrice TOURSEL ,  Francois DELANNOY ,  Samuel PATINIER

    IPC分类号： C12N1/06 ,  B02C17/20 ,  C12N1/12

    CPC分类号： C12N1/066 ,  B02C17/16 ,  B02C17/20 ,  B02C23/00 ,  B02C23/02 ,  B02C25/00 ,  C12M21/02 ,  C12M47/06 ,  C12N1/12

    摘要： Disclosed is a method of mechanically crushing microalgae cells of the genus Chlorella at an industrial scale, the mechanical crushing being carried out in a horizontal ball mill system. In the method, the balls have an apparent density of between 2 and 3.5 kg/1, the filling rate of the crushing chamber is greater than or equal to 80%, and preferably greater than or equal to 85%, and the mechanical crushing is carried out continuously by a series of successive passes.

    摘要翻译： 公开了以工业规模机械破碎小球藻属微藻细胞的方法，机械破碎是在水平球磨机系统中进行的。 在该方法中，球的表观密度为2〜3.5kg / l，破碎室的填充率为80％以上，优选为85％以上，机械破碎为 通过一系列连续通行持续进行。

    Assays for pathogen detection using microwaves for lysing and accelerating metal-enhanced fluorescence
    67.
    发明授权
    Assays for pathogen detection using microwaves for lysing and accelerating metal-enhanced fluorescence 有权
    使用微波进行病原体检测，用于裂解和加速金属增强荧光

    公开(公告)号：US09500590B2

    公开(公告)日：2016-11-22

    申请号：US13990828

    申请日：2011-12-01

    申请人： Chris D. Geddes

    发明人： Chris D. Geddes

    IPC分类号： C12Q1/68 ,  C07H21/00 ,  G01N1/34 ,  G01N21/64 ,  C12N1/06 ,  C12N13/00 ,  C12Q1/04

    CPC分类号： G01N21/6486 ,  C12N1/066 ,  C12N13/00 ,  C12Q1/04 ,  G01N21/648

    摘要： The present invention relates to an assay for the determination of a microorganism including a lysing chamber having triangular shaped metallic structures wherein the apexes of two triangles are arranged in alignment and forming a reactive zone for placement of the microorganism and lysing by microwave energy for exposing and isolating a target polynucleotide sequence. The isolated target polynucleotide sequence is introduced to an assay system for contact with polynucleotides which are complimentary to the isolated target polynucleotide sequence. Fluorophore-labeled capture polynucleotides are added for hybridizing to any bound target polynucleotide. Bound target polynucleotides are detected by metal enhanced fluorescence.

    摘要翻译： 本发明涉及一种测定微生物的方法，该微生物包括具有三角形金属结构的裂解室，其中两个三角形的顶点排成一列并形成用于放置微生物的反应区和通过微波裂解裂解以暴露和 分离靶多核苷酸序列。 将分离的靶多核苷酸序列引入到与分离的靶多核苷酸序列互补的多核苷酸的测定系统中。 添加荧光团标记的捕获多核苷酸用于与任何结合的靶多核苷酸杂交。 通过金属增强荧光检测结合靶多核苷酸。

    E. coli plasmid DNA production
    68.
    发明授权
    E. coli plasmid DNA production 有权
    大肠杆菌质粒DNA生产

    公开(公告)号：US09487789B2

    公开(公告)日：2016-11-08

    申请号：US14612960

    申请日：2015-02-03

    申请人： NATURE TECHNOLOGY CORPORATION

    发明人： James A. Williams ,  Aaron E. Carnes

    IPC分类号： C12P21/06 ,  C12N15/73 ,  C12N1/06 ,  C12N15/10 ,  C12N15/64 ,  C12P21/04 ,  C12N15/74

    CPC分类号： C12N15/64 ,  C12N1/06 ,  C12N15/1003 ,  C12N15/1017 ,  C12N15/73

    摘要： General methods and strains of bacteria are described that dramatically simplify and streamline plasmid DNA production. In one preferred embodiment, endolysin mediated plasmid extraction combined with flocculation mediated removal of cell debris and host nucleic acids achieves increased yield and purity with simplified downstream purification and reduced waste streams, thus reducing production costs.

    摘要翻译： 描述了一般方法和细菌菌株，其显着简化和简化质粒DNA生产。 在一个优选的实施方案中，细胞内溶素介导的质粒提取结合絮凝介导的细胞碎片和宿主核酸的去除通过简化的下游纯化和减少的废物流实现了增加的产量和纯度，从而降低了生产成本。

    METHOD OF EXTRACTING ORGANELLES FROM CELLS
    69.
    发明申请
    METHOD OF EXTRACTING ORGANELLES FROM CELLS 有权
    从细胞提取有机物的方法

    公开(公告)号：US20160257924A1

    公开(公告)日：2016-09-08

    申请号：US14844692

    申请日：2015-09-03

    申请人： TAIWAN MITOCHONDRION APPLIED TECHNOLOGY CO., LTD.

    发明人： Chia-Wen Lin ,  Hsin-Ting Tsai ,  Han-Chung Cheng

    IPC分类号： C12N1/06

    CPC分类号： C12N5/00

    摘要： A method of extracting organelles from cells includes following process. A plurality of cells and a solution are mixed uniformly to obtain a mixture. The mixture is loaded into a container. The container is placed in a milling device comprising a miller and a mechanical power source connected to the miller. The miller is immersed into the mixture within the container. The mixture is milled by the miller to obtain a homogenized mixture, wherein a milling parameter is provided to the milling device, and the mechanical power source drives the miller according to the milling parameter. The homogenized mixture is centrifuged to obtain a supernatant. The supernatant is centrifuged to obtain a precipitate with a plurality of organelles. In the process that the mixture is milled by the miller, at least a part of the miller is kept in the mixture.

    摘要翻译： 从细胞中提取细胞器的方法包括以下过程。 将多个电池和溶液均匀混合以获得混合物。 将混合物装入容器中。 将容器放置在包括铣刀和连接到铣刀的机械动力源的铣削装置中。 将磨机浸入容器内的混合物中。 混合物由研磨机研磨以获得均质混合物，其中铣削参数提供给研磨装置，机械动力源根据研磨参数驱动铣刀。 将匀化的混合物离心以获得上清液。 将上清液离心以获得具有多个细胞器的沉淀物。 在混合物由研磨机研磨的过程中，将磨机的至少一部分保持在混合物中。