公开(公告)号：US20180208969A1

公开(公告)日：2018-07-26

申请号：US15870176

申请日：2018-01-12

申请人： Roche Molecular Systems, Inc.

发明人： Meike Eickhoff ,  Eberhard Russmann ,  Andreas Woelfelschneider ,  Dirk Zimmerman

IPC分类号： C12Q1/6806

CPC分类号： C12Q1/6806 ,  C12Q1/6844 ,  C12Q1/6851 ,  C12Q1/703 ,  C12Q1/706 ,  C12Q2565/518 ,  C12Q2545/101 ,  C12Q2537/143 ,  C12Q2561/113 ,  C12Q2545/113

摘要： The present invention belongs to the field of in-vitro diagnostics. Within this field, it particularly concerns the amplification of at least a first target nucleic acid that may be present in at least one fluid sample using an internal control nucleic acid for qualitative and/or quantitative purposes and at least one external control nucleic acid in an aqueous buffer. It further provides an analytical system comprising an internal control nucleic acid for qualitative and/or quantitative purposes and at least one external control nucleic acid in an aqueous buffer.

公开(公告)号：US10023917B2

公开(公告)日：2018-07-17

申请号：US14958567

申请日：2015-12-03

申请人： Roche Molecular Systems, Inc.

发明人： Reinhard Buettner ,  Stefanie Froehner ,  Sabine Merkelbach-Bruse ,  Jasmin Ney ,  Angelika Roesler

IPC分类号： C12Q1/68 ,  C12Q1/6886 ,  C12N9/14

摘要： Compositions and methods for determining an increased likelihood of a response to a targeted treatment of a cancer disease including isolating genomic DNA from a patient sample, amplifying a fragment of DNA by means of PCR with a specific pair of amplification primers, determining if the amplified fragment comprises a wildtype sequence or a mutation by means of a High Resolution Melting Analysis (HRM), and correlating the presence or absence of a mutation with an increased likelihood of success of said targeted treatment. Respective primer pairs, compositions and kits are also claimed.

公开(公告)号：US10023850B2

公开(公告)日：2018-07-17

申请号：US15674327

申请日：2017-08-10

申请人： Roche Molecular Systems, Inc.

发明人： Fred Reichert ,  Keith Bauer ,  Thomas W. Myers

IPC分类号： C12N9/12 ,  C12Q1/6806

摘要： Disclosed are mutant DNA polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.

公开(公告)号：US10023849B2

公开(公告)日：2018-07-17

申请号：US15331748

申请日：2016-10-21

申请人： Roche Molecular Systems, Inc.

发明人： Shawn Suko

IPC分类号： C12N9/12 ,  C12N1/21 ,  C12N15/54 ,  C12P19/34

摘要： Disclosed are DNA polymerases having increased reverse transcriptase efficiency relative to a corresponding, unmodified polymerase. The polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the DNA polymerases.

公开(公告)号：US10022722B2

公开(公告)日：2018-07-17

申请号：US14676662

申请日：2015-04-01

申请人： Roche Molecular Systems, Inc.

发明人： Shuqi Chen ,  Lingjun Chen

IPC分类号： B01L3/00 ,  A61B5/155 ,  A61B5/15

摘要： A sample vessel may include a segmented tubule having an opening for receiving a sample material and at least one compressible section, the at least one compressible section having a wall constructed at least partially from a material having sufficient flexibility to permit compression of opposed sections of the wall into contact, and at least two segments of the tubule being fluidically isolated from one another by a bonding of opposed sections of the tubule wall to one another. The sample vessel may also include an interface in fluid communication with the opening in the tubule, the interface facilitating delivery of a sample material to the tubule through the opening.

公开(公告)号：US20180135034A1

公开(公告)日：2018-05-17

申请号：US15862427

申请日：2018-01-04

申请人： Roche Molecular Systems, Inc.

发明人： Keith Bauer ,  Fred Reichert ,  Thomas W. Myers

IPC分类号： C12N9/12 ,  C12Q1/6806

CPC分类号： C12N9/1252 ,  C12Q1/6806 ,  C12Y207/07007

摘要： Disclosed are mutant DNA polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.

公开(公告)号：US20180119116A1

公开(公告)日：2018-05-03

申请号：US15804489

申请日：2017-11-06

申请人： Roche Molecular Systems, Inc.

发明人： Fred REICHERT ,  Keith BAUER ,  Thomas W. MYERS

IPC分类号： C12N9/12 ,  C12P19/34 ,  C12Q1/6827 ,  C12Q1/6848 ,  C12Q1/6858 ,  C12Q1/686

CPC分类号： C12N9/1252 ,  C12P19/34 ,  C12Q1/6827 ,  C12Q1/6848 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Y207/07007 ,  C12Q2521/101 ,  C12Q2535/125

摘要： Disclosed are mutant DNA polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.

公开(公告)号：US20180095046A1

公开(公告)日：2018-04-05

申请号：US15717720

申请日：2017-09-27

申请人： Roche Molecular Systems, Inc.

发明人： Claudio Cherubini ,  Michal Dobrzynski ,  Paul Federer ,  Thomas Kern ,  Lukas Schneider

IPC分类号： G01N27/22 ,  G01V3/08 ,  G01F23/26 ,  B01L3/00

摘要： A sensor (100) for detecting a state of a fluid within a microfluidic plate (120) is disclosed. The sensor (100) comprises at least a first planar electrode (102) and a second planar electrode (104) arranged parallel to one another so as to form an electric capacitor (106). The first planar electrode (102) and the second planar electrode (104) are configured to generate an electric field (114) within the fluid, wherein the sensor (100) is configured to detect the state of the fluid by means of a capacitance of the electric capacitor (106). Further, a system (116) for controlling a preparation of a sample including a fluid is disclosed. Furthermore, a method for detecting a state of a fluid within a microfluidic plate (120) is disclosed.

公开(公告)号：US09932574B2

公开(公告)日：2018-04-03

申请号：US15611527

申请日：2017-06-01

申请人： Roche Molecular Systems, Inc.

发明人： Geza Burghardt ,  Willem Mulder ,  Rolf Schneebeli

IPC分类号： B01L3/00 ,  C12N15/10 ,  B01F15/00 ,  B03C1/01 ,  B03C1/28 ,  B01F11/00 ,  G01N1/40 ,  B01L3/02 ,  B01L7/00

CPC分类号： C12N15/1006 ,  B01F11/0022 ,  B01F15/00733 ,  B01F15/00876 ,  B01L3/021 ,  B01L3/50 ,  B01L3/508 ,  B01L7/52 ,  B01L2200/0647 ,  B01L2200/141 ,  B01L2300/044 ,  B01L2300/0672 ,  B01L2300/0829 ,  B01L2300/0832 ,  B01L2400/043 ,  B01L2400/0457 ,  B03C1/01 ,  B03C1/288 ,  B03C2201/26 ,  G01N1/405

摘要： A device, method and system is provided for binding particles for the separation and/or isolation of biological materials. In particular, a container is provided including a suspension of binding particles for the isolation of biological material, inner walls forming an elongate groove at the bottom of said container, and a cover having openings and/or being penetrable for a linear arrangement of multiple pipets or pipet tips, said openings being located above and parallel to said elongate groove.

公开(公告)号：US20180056297A1

公开(公告)日：2018-03-01

申请号：US15693009

申请日：2017-08-31

申请人： Roche Molecular Systems, Inc.

发明人： Sven Ebert ,  Paul Federer ,  Thomas Schlaubitz ,  Stefan Vollenweider ,  Urs Wittenwiler

IPC分类号： B01L7/00 ,  C12P19/34 ,  B01L9/06

CPC分类号： B01L7/52 ,  B01L3/5085 ,  B01L9/06 ,  B01L2200/025 ,  B01L2300/0829 ,  B01L2300/1805 ,  B01L2300/1822 ,  B01L2300/1894 ,  C12P19/34 ,  H01L23/38 ,  H01L27/16 ,  H05B3/10

摘要： An assembly (10) comprising a sample block (12), a heat sink (30) and at least one electrodeposited thermoelectric element (48) is disclosed. Further, an instrument and a method for performing a temperature-dependent reaction are disclosed.