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公开(公告)号：US10006055B2
公开(公告)日：2018-06-26
申请号：US14869872
申请日：2015-09-29
Applicant: Genomatica, Inc.
Inventor： Mark J. Burk , Anthony P. Burgard , Robin E. Osterhout , Jun Sun , Priti Pharkya
Abstract: The invention provides non-naturally occurring microbial organisms having a butadiene or crotyl alcohol pathway. The invention additionally provides methods of using such organisms to produce butadiene or crotyl alcohol.
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公开(公告)号：US09982281B2
公开(公告)日：2018-05-29
申请号：US14692630
申请日：2015-04-21
Applicant: Genomatica, Inc.
Inventor： Anthony P. Burgard , Mark J. Burk , Robin E. Osterhout , Jun Sun , Priti Pharkya
Abstract: Provided herein is a non-naturally occurring microbial organism having a 1,3-butanediol (1,3-BDO) pathway and comprising at least one exogenous nucleic acid encoding a 1,3-BDO pathway enzyme expressed in a sufficient amount to produce 1,3-BDO. In some embodiments, the pathway includes reducing equivalents from CO or hydrogen. In certain embodiments, a 1,3-BDO pathway proceeds by way of central metabolites pyruvate, succinate or alpha-ketoglutarate. Also provided herein is a method for producing 1,3-BDO, includes culturing such microbial organisms under conditions and for a sufficient period of time to produce 1,3-BDO.
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73.发明授权公开(公告)号：US09885064B2
公开(公告)日：2018-02-06
申请号：US14185709
申请日：2014-02-20
Applicant: Genomatica, Inc.
Inventor： Mark J. Burk , Christophe H. Schilling , Anthony P. Burgard , John D. Trawick
IPC: C12N1/20 , C12N1/12 , C12P7/62 , C12P7/04 , C07H21/04 , C12P7/18 , C12N9/02 , C12N9/10 , C12N9/00 , C12P19/32 , C12N15/70
CPC classification number: C12P7/18 , C12N9/0008 , C12N9/0036 , C12N9/1007 , C12N9/93 , C12N15/70 , C12P19/32 , Y02E50/343
Abstract: The invention provides a non-naturally occurring microbial organism having an acetyl-CoA pathway and the capability of utilizing syngas or syngas and methanol. In one embodiment, the invention provides a non-naturally occurring microorganism, comprising one or more exogenous proteins conferring to the microorganism a pathway to convert CO, CO2 and/or H2 to acetyl-coenzyme A (acetyl-CoA), methyl tetrahydrofolate (methyl-THF) or other desired products, wherein the microorganism lacks the ability to convert CO or CO2 and H2 to acetyl-CoA or methyl-THF in the absence of the one or more exogenous proteins. For example, the microbial organism can contain at least one exogenous nucleic acid encoding an enzyme or protein in an acetyl-CoA pathway. The microbial organism is capable of utilizing synthesis gases comprising CO, CO2 and/or H2, alone or in combination with methanol, to produce acetyl-CoA. The invention additionally provides a method for producing acetyl-CoA, for example, by culturing an acetyl-CoA producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding an acetyl-CoA pathway enzyme or protein in a sufficient amount to produce acetyl-CoA, under conditions and for a sufficient period of time to produce acetyl-CoA.
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公开(公告)号：US09732361B2
公开(公告)日：2017-08-15
申请号：US14059131
申请日：2013-10-21
Applicant: Genomatica, Inc.
Inventor： Mark J. Burk , Anthony P. Burgard , Jun Sun , Robin E. Osterhout , Priti Pharkya
CPC classification number: C12P5/026 , C07C11/16 , C08F136/06 , C12N15/52
Abstract: The invention provides non-naturally occurring microbial organisms having a butadiene pathway. The invention additionally provides methods of using such organisms to produce butadiene.
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公开(公告)号：US20160053286A1
公开(公告)日：2016-02-25
申请号：US14673600
申请日：2015-03-30
Applicant: Genomatica, Inc.
Inventor： Anthony P. Burgard , Mark J. Burk , Robin E. Osterhout , Priti Pharkya
CPC classification number: C12P7/18 , C07C29/80 , C12N9/0006 , C12N9/0008 , C12N9/1029 , C12N9/1096 , C12N9/88 , C12N15/52 , C12Y101/01 , C12Y101/01157 , C12Y102/01 , C12Y203/01 , C12Y206/01 , C12Y401/01 , C12Y402/01 , C12Y403/01
Abstract: A non-naturally occurring microbial organism includes a microbial organism having a 1,3-butanediol (1,3-BDO) pathway having at least one exogenous nucleic acid encoding a 1,3-BDO pathway enzyme expressed in a sufficient amount to produce 1,3-BDO. The pathway includes an enzyme selected from a 2-amino-4-ketopentanoate (AKP) thiolase, an AKP dehydrogenase, a 2-amino-4-hydroxypentanoate aminotransferase, a 2-amino-4-hydroxypentanoate oxidoreductase (deaminating), a 2-oxo-4-hydroxypentanoate decarboxylase, a 3-hydroxybutyraldehyde reductase, an AKP aminotransferase, an AKP oxidoreductase (deaminating), a 2,4-dioxopentanoate decarboxylase, a 3-oxobutyraldehyde reductase (ketone reducing), a 3-oxobutyraldehyde reductase (aldehyde reducing), a 4-hydroxy-2-butanone reductase, an AKP decarboxylase, a 4-aminobutan-2-one aminotransferase, a 4-aminobutan-2-one oxidoreductase (deaminating), a 4-aminobutan-2-one ammonia-lyase, a butenone hydratase, an AKP ammonia-lyase, an acetylacrylate decarboxylase, an acetoacetyl-CoA reductase (CoA-dependent, aldehyde forming), an acetoacetyl-CoA reductase (CoA-dependent, alcohol forming), an acetoacetyl-CoA reductase (ketone reducing), a 3-hydroxybutyryl-CoA reductase (aldehyde forming), a 3-hydroxybutyryl-CoA reductase (alcohol forming), a 4-hydroxybutyryl-CoA dehydratase, and a crotonase. A method for producing 1,3-BDO, includes culturing such microbial organisms under conditions and for a sufficient period of time to produce 1,3-BDO.
Abstract translation: 非天然存在的微生物生物体包括具有1,3-丁二醇（1,3-BDO）途径的微生物生物，其具有至少一种编码1,3-BDO途径酶的外源核酸，所述外源核酸以足够的量表达，以产生1 ，3-BDO。 该途径包括选自2-氨基-4-酮戊酸酯（AKP）硫解酶，AKP脱氢酶，2-氨基-4-羟基戊酸转氨酶，2-氨基-4-羟基戊酸酯氧化还原酶（脱氨基），2- 4-羟基戊酸脱羧酶，3-羟基丁醛还原酶，AKP氨基转移酶，AKP氧化还原酶（脱氨），2,4-二氧代戊酸脱羧酶，3-氧代丁醛还原酶（还原酮），3-氧代丁醛还原酶 ），4-羟基-2-丁酮还原酶，AKP脱羧酶，4-氨基丁-2-酮氨基转移酶，4-氨基丁-2-酮氧化还原酶（脱氨基），4-氨基丁-2-酮氨裂解酶 乙酰乙酰辅酶A还原酶（CoA依赖性醛形成），乙酰乙酰辅酶A还原酶（CoA依赖性醇形成），乙酰乙酰辅酶A还原酶（酮），乙酰乙酰辅酶A还原酶 还原），3-羟基丁酰辅酶A还原酶（醛形成），3-羟基 Ryl-CoA还原酶（醇形成），4-羟基丁酰辅酶A脱水酶和巴豆酸酶。 一种生产1,3-BDO的方法包括在条件和足够的时间内培养这些微生物生产1,3-BDO。
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Patent Agency Ranking
公开(公告)号：US20140322777A1
公开(公告)日：2014-10-30
申请号：US14066598
申请日：2013-10-29
Applicant: Genomatica, Inc.
Inventor： Warren Clark , Michael Japs , Mark J. Burk
CPC classification number: C07C29/76 , C12P7/18 , C07C31/207
Abstract: A process of isolating 1,4-butanediol (1,4-BDO) from a fermentation broth includes separating a liquid fraction enriched in 1,4-BDO from a solid fraction comprising cells, removing water from said liquid fraction, removing salts from said liquid fraction, and purifying 1,4-BDO. A process for producing 1,4-BDO includes culturing a 1,4-BDO-producing microorganism in a fermentor for a sufficient period of time to produce 1,4-BDO. The 1,4-BDO-producing microorganism includes a microorganism having a 1,4-BDO pathway having one or more exogenous genes encoding a 1,4-BDO pathway enzyme and/or one or more gene disruptions. The process for producing 1,4-BDO further includes isolating 1,4-BDO.
Abstract translation: 从发酵液中分离1,4-丁二醇（1,4-BDO）的方法包括从含有细胞的固体部分中分离富含1,4-BDO的液体级分，从所述液体馏分中除去水，从所述 液体馏分，并纯化1,4-BDO。 生产1,4-BDO的方法包括在发酵罐中培养产生1,4-BDO的微生物足够长的时间以产生1,4-BDO。 产生1,4-BDO的微生物包括具有1,4-BDO途径的微生物，其具有编码1,4-BDO途径酶的一种或多种外源基因和/或一种或多种基因中断。 生产1,4-BDO的方法还包括分离1,4-BDO。
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公开(公告)号：US20130316426A1
公开(公告)日：2013-11-28
申请号：US13887140
申请日：2013-05-03
Applicant: Genomatica, Inc.
Inventor： Mark J. Burk , Priti Pharkya , Stephen J. Van Dien , Anthony P. Burgard , Christophe H. Schilling
CPC classification number: C07C51/353 , C07C51/38 , C07C57/04 , C07C67/08 , C07C67/32 , C07C67/333 , C12P7/40 , C12P7/46 , C12P7/62 , Y02P20/125 , C07C69/60 , C07C69/54
Abstract: The invention provides a method of producing acrylic acid. The method includes contacting fumaric acid with a sufficient amount of ethylene in the presence of a cross-metathesis transformation catalyst to produce about two moles of acrylic acid per mole of fumaric acid. Also provided is an acrylate ester. The method includes contacting fumarate diester with a sufficient amount of ethylene in the presence of a cross-metathesis transformation catalyst to produce about two moles of acrylate ester per mole of fumarate diester. An integrated process for process for producing acrylic acid or acrylate ester is provided which couples bioproduction of fumaric acid with metathesis transformation. An acrylic acid and an acrylate ester production also is provided.
Abstract translation: 本发明提供了制备丙烯酸的方法。 该方法包括在交叉复分解转化催化剂存在下使富马酸与足够量的乙烯接触，以产生约2摩尔丙烯酸/摩尔富马酸。 还提供了丙烯酸酯。 该方法包括在交叉复分解转化催化剂存在下使富马酸二酯与足够量的乙烯接触，以产生每摩尔富马酸二酯约2摩尔丙烯酸酯。 提供了用于生产丙烯酸或丙烯酸酯的方法的综合方法，其将富马酸的生物生产与易位转化相结合。 还提供丙烯酸和丙烯酸酯生产。
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公开(公告)号：US20240182935A1
公开(公告)日：2024-06-06
申请号：US18321585
申请日：2023-05-22
Applicant: Genomatica, Inc.
Inventor： Lauri H. Suominen , Connor J. Galleher , Michael Japs , Mark J. Burk , Cara Tracewell
CPC classification number: C12P13/001 , C12N1/02 , C12N1/20 , C12N9/88 , C12N9/96 , C12N11/16 , C12N15/52 , C12Y402/01001
Abstract: Provided is a method of producing and isolating a diamine produced by microbial fermentation that minimizes undesirable salt formation to provide a lower cost process.
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公开(公告)号：US11814664B2
公开(公告)日：2023-11-14
申请号：US14893510
申请日：2014-05-23
Applicant: Genomatica, Inc.
Inventor： Benjamin Adelstein , Robin E. Osterhout , Mark J. Burk
IPC: C12P7/62 , C12N9/18 , A61K31/22 , A61K35/66 , A61K36/064 , A23L33/135 , C12N1/00 , C12N1/14 , C12N1/20 , C07C69/675 , C12N15/52 , C12R1/00 , C12R1/01 , C12R1/645
CPC classification number: C12P7/62 , A23L33/135 , A61K31/22 , A61K35/66 , A61K36/064 , C07C69/675 , C12N1/00 , C12N1/145 , C12N1/205 , C12N9/18 , C12N15/52 , C12Y101/011 , C12Y101/01035 , C12Y301/01067 , C12Y301/08001 , A23V2002/00 , C07B2200/05 , C12R2001/00 , C12R2001/01 , C12R2001/645 , Y02P20/10
Abstract: Provided herein are non-naturally occurring microbial organisms having a pathway for production of (3R)-hydroxybutyl (3R)-hydroxybutyrate, wherein the organism can further include a (R)-1,3-butanediol pathway, a (3R)-hydroxybutyrate pathway, a (3R)-hydroxybutyryl-CoA pathway, an acetoacetate pathway, an acetoacetyl-CoA pathway, a (3R)-hydroxybutyl-ACP pathway, or an acetoacetyl-ACP pathway. Additionally provided are methods and processes for producing and isolating (3R)-hydroxybutyl (3R)-hydroxybutyrate using the microbial organisms, and various compositions having the (3R)-hydroxybutyl (3R)-hydroxybutyrate. Still further provided are methods of treating or preventing a disease, disorder or condition using the (3R)-hydroxybutyl (3R)-hydroxybutyrate produced by the microbial organisms of the invention.
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公开(公告)号：US20230287464A1
公开(公告)日：2023-09-14
申请号：US17749529
申请日：2022-05-20
Applicant: Genomatica, Inc.
Inventor： Priti Pharkya , Anthony P. Burgard , Mark J. Burk
IPC: C12P5/02 , C12N15/52 , C12P3/00 , C12P7/40 , C01B3/00 , C08F36/06 , C08F36/14 , C12N9/04 , C12N9/02 , C12N9/10 , C12N9/12
CPC classification number: C12P5/026 , C01B3/00 , C08F36/06 , C08F36/14 , C12N9/0006 , C12N9/0008 , C12N9/10 , C12N9/12 , C12N9/0067 , C12N15/52 , C12P3/00 , C12P7/40 , C12Y101/02007 , C12Y101/03013 , C12Y102/99003 , Y02E50/30 , Y02E60/32
Abstract: The invention provides non-naturally occurring microbial organisms containing butadiene or 2,4-pentadienoate pathways comprising at least one exogenous nucleic acid encoding a butadiene or 2,4-pentadienoate pathway enzyme expressed in a sufficient amount to produce butadiene or 2,4-pentadienoate. The organism can further contain a hydrogen synthesis pathway. The invention additionally provides methods of using such microbial organisms to produce butadiene or 2,4-pentadienoate by culturing a non-naturally occurring microbial organism containing butadiene or 2,4-pentadienoate pathways as described herein under conditions and for a sufficient period of time to produce butadiene or 2,4-pentadienoate. Hydrogen can be produced together with the production of butadiene or 2,4-pentadienoate.
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