公开(公告)号：US20040153249A1

公开(公告)日：2004-08-05

申请号：US10635241

申请日：2003-08-05

Applicant: The Johns Hopkins University

Inventor： Zhen Zhang ,  Daniel Wanyui Chan

IPC: G06F015/18 ,  G06F019/00 ,  G01N033/48 ,  G01N033/50

CPC classification number: G01N33/6803 ,  C12Q1/6876 ,  G16B20/00 ,  G16B25/00 ,  G16B40/00 ,  H01J49/00

Abstract: The invention provides a method, system and software to screen for, identify and validate biomarkers that are predictive of a biological state, such as a cell state and/or patient status.

Abstract translation: 本发明提供了一种方法，系统和软件，用于筛选，鉴定和验证预测生物状态的细胞状态和/或患者状态的生物标志物。

公开(公告)号：US20040076549A1

公开(公告)日：2004-04-22

申请号：US10274247

申请日：2002-10-18

Inventor： Tony J. Tegeler ,  Yehia Mechref ,  Kirk S. Boraas ,  James P. Reilly ,  Milos V. Novotny

IPC: B01L003/02

CPC classification number: B01L3/0268 ,  B01L2300/0812 ,  B01L2400/0487 ,  G01N30/84 ,  G01N2030/8417 ,  H01J49/00 ,  Y10T436/11 ,  Y10T436/110833 ,  Y10T436/112499 ,  Y10T436/113332 ,  Y10T436/25 ,  Y10T436/2575

Abstract: A device for placement of effluent comprises a substrate positioner, a deposition conduit, and a conduit positioner. The substrate positioner supports and positions a substrate on which effluent exiting from the deposition conduit is to be deposited. The conduit positioner moves an exit end of the deposition conduit relative to the substrate.

Abstract translation: 用于放置流出物的装置包括基板定位器，沉积导管和导管定位器。 衬底定位器支撑并定位要沉积从沉积导管排出的流出物的衬底。 管道定位器相对于衬底移动沉积管道的出口端。

公开(公告)号：US06720190B1

公开(公告)日：2004-04-13

申请号：US09276444

申请日：1999-03-25

Applicant: Ole Hindsgaul ,  David C. Schriemer

Inventor： Ole Hindsgaul ,  David C. Schriemer

IPC: G01N3002

CPC classification number: G01N33/68 ,  B01J2219/00585 ,  B01J2219/00704 ,  B01J2219/00707 ,  B01J2219/00731 ,  B01J2219/00738 ,  B01J2219/00745 ,  B01J2219/00747 ,  C40B30/08 ,  C40B40/12 ,  C40B40/18 ,  G01N30/466 ,  G01N30/7266 ,  G01N33/538 ,  G01N33/54306 ,  G01N33/54366 ,  G01N33/6845 ,  G01N33/6848 ,  G01N2030/628 ,  H01J49/00 ,  H01J49/0036 ,  Y10T436/24

Abstract: Disclosed are methods for screening compound libraries using frontal chromatography in combination with mass spectrometry to identify and rank those members of the library that bind to a target receptor. Methods are also disclosed which permit a compound library to be rapidly screened to determine if any member of the library has an affinity for the target receptor as measured by a pre-selected indicator compound.

公开(公告)号：US20030228700A1

公开(公告)日：2003-12-11

申请号：US10289009

申请日：2002-11-05

Applicant: IRM LLC

Inventor： Eric C. Peters ,  Ansgar Brock ,  Christer Ericson

IPC: G01N033/00

CPC classification number: C07K1/13 ,  C07K14/805 ,  G01N33/6848 ,  G01N2035/00158 ,  G01N2458/15 ,  G16B30/00 ,  G16B50/00 ,  H01J49/00 ,  H01J49/0027 ,  H01J49/38 ,  Y10T436/24

Abstract: The present invention provides compounds which are useful as multifunctional labels in proteomics studies. The labels of the present invention are both lysine specific and increase the overall sequence coverage obtained in polypeptide mapping experiments, by for example, increasing the ionization efficiencies of lysine-terminated tryptic fragments. In certain aspects, the labels of the present invention can be used to measure differential quantitation, as for example, deuterium(s) can easily be introduced during their synthesis. In one aspect, a C-terminal derivatized lysine biases the fragment ion intensities strongly toward C-terminal fragment ions, resulting in a highly simplified tandem mass spectrum. In further aspects, the number of lysine residues can be determined in a polypeptide.

Abstract translation: 本发明提供在蛋白质组学研究中可用作多功能标记的化合物。 本发明的标记物都是赖氨酸特异性的，并且通过例如提高赖氨酸终止的胰蛋白酶片段的离子化效率来增加多肽测绘实验中获得的整体序列覆盖率。 在某些方面，本发明的标记可以用于测量差异定量，例如，在合成过程中可以容易地引入氘。 在一个方面，C末端衍生的赖氨酸强烈地将片段离子强度偏向C末端片段离子，导致高度简化的串联质谱。 在另外的方面，可以在多肽中确定赖氨酸残基的数目。

公开(公告)号：US06613575B1

公开(公告)日：2003-09-02

申请号：US09571268

申请日：2000-05-15

Applicant: Ole Hindsgaul ,  David C. Schriemer

Inventor： Ole Hindsgaul ,  David C. Schriemer

IPC: G01N3002

CPC classification number: G01N33/538 ,  B01J2219/00585 ,  B01J2219/00704 ,  B01J2219/00707 ,  B01J2219/00731 ,  B01J2219/00738 ,  B01J2219/00745 ,  B01J2219/00747 ,  C40B30/08 ,  C40B40/12 ,  C40B40/18 ,  G01N30/466 ,  G01N30/7266 ,  G01N33/54306 ,  G01N33/54366 ,  G01N33/68 ,  G01N2030/628 ,  H01J49/00 ,  Y10T436/24

Abstract: Disclosed are methods for screening compound libraries using frontal chromatography in combination with mass spectrometry to identify and rank those members of the library that bind to a target receptor. Methods are also disclosed which permit a compound library to be rapidly screened to determine if any members of the library have a higher affinity for the target receptor relative to a pre-selected indicator compound.

公开(公告)号：US06589485B2

公开(公告)日：2003-07-08

申请号：US09879341

申请日：2001-06-11

Applicant: Hubert Köster

Inventor： Hubert Köster

IPC: B01L900

CPC classification number: C12Q1/6872 ,  C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/6837 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6862 ,  C12Q1/6883 ,  C12Q1/706 ,  G01N35/1067 ,  H01J49/00 ,  Y10T436/143333 ,  Y10T436/24 ,  C12Q2565/627 ,  C12Q2537/113 ,  C12Q2535/113 ,  C12Q2521/325 ,  C12Q2535/131 ,  C12Q2525/107 ,  C12Q2523/107 ,  C12Q2523/319 ,  C12Q2565/625 ,  C12Q2535/125

Abstract: Fast and highly accurate mass spectrometry-based processes for detecting particular nucleic acid molecules and sequences in the molecules are provided. Arrays of oligonucleotides for performing mass spectrometric analyses are provided. In one aspect, a solid support is provided that includes a plurality of single-stranded oligonucleotides immobilized thereon by a linker cleavable under conditions of matrix-assisted laser desorption/ionization (MALDI) mass spectrometry; and matrix for performing MALDI mass spectrometry, wherein the support comprises a flat surface.

Abstract translation: 提供了用于检测分子中特定核酸分子和序列的基于质谱的快速和高精度的方法。 提供了用于进行质谱分析的寡核苷酸阵列。 在一个方面，提供了固体支持物，其包括通过在基质辅助激光解吸/电离（MALDI）质谱法的条件下可切割的接头固定在其上的多个单链寡核苷酸; 和用于进行MALDI质谱分析的基质，其中所述载体包括平坦表面。

公开(公告)号：US20030104483A1

公开(公告)日：2003-06-05

申请号：US10301110

申请日：2002-11-21

Applicant: Boehringer Ingelheim Pharmaceuticals, Inc.

Inventor： Walter Carroll Davidson ,  Lee Frego

IPC: G01N033/53 ,  C12Q001/37 ,  G01N033/00

CPC classification number: G01N33/6848 ,  B01D15/325 ,  C12Q1/37 ,  G01N30/7233 ,  H01J49/00

Abstract: ECD (Electron Capture Dissociation) FTMS (Fourier-Transform Mass Spectrometry) induced fragmentation is employed to generate sequence information for a protein enzymatic digest. The digest is initially separated by liquid chromatography, e.g., reversed phase nullHPLC, and then ionized nullon-linenull. The ions thus formed may be accumulated in the interface hexapole prior to injection and trapping in the FTMS cell. Typically, no parent ion isolation is performed. The trapped ions are subjected to a pulse of electrons to induce fragmentation. Broad band spectra are acquired continuously to produce a three-dimensional LC/MS data set. The spectra are dominated by c and to a lesser degree z ions, which provide nearly complete sequence coverage. External calibration provides good mass accuracy and resolution, typical of FTMS. Thus, LC/ECD-FTMS is shown to be a highly informative method for the analysis of enzymatic protein digests.

Abstract translation: 使用ECD（电子俘获解离）FTMS（傅里叶变换质谱）诱导的片段化产生蛋白酶消化的序列信息。 消化液最初通过液相色谱分离，例如反相muHPLC，然后电离“在线”。 这样形成的离子可以在注入之前积聚在界面六极中并在FTMS单元中捕获。 通常，不进行母离子隔离。 被俘获的离子受到电子脉冲的诱导分裂。 连续获取宽带光谱以产生三维LC / MS数据集。 光谱由c和较少的z离子支配，其提供几乎完整的序列覆盖。 外部校准提供了良好的质量精度和分辨率，典型的FTMS。 因此，LC / ECD-FTMS被证明是用于分析酶蛋白质消化的高度信息的方法。

公开(公告)号：US06500621B2

公开(公告)日：2002-12-31

申请号：US09796416

申请日：2001-02-28

Applicant: Hubert Köster

Inventor： Hubert Köster

IPC: C12Q168

CPC classification number: C12Q1/6872 ,  C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/6837 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6862 ,  C12Q1/6883 ,  C12Q1/706 ,  G01N35/1067 ,  H01J49/00 ,  Y10T436/143333 ,  Y10T436/24 ,  C12Q2565/627 ,  C12Q2537/113 ,  C12Q2535/113 ,  C12Q2521/325 ,  C12Q2535/131 ,  C12Q2525/107 ,  C12Q2523/107 ,  C12Q2523/319 ,  C12Q2565/625 ,  C12Q2535/125

Abstract: Fast and highly accurate mass spectrometry-based processes for detecting particular nucleic acid molecules and sequences in the molecules are provided. Depending upon the sequence to be detected, the processes, for example, can be used to diagnose a genetic disease or a chromosomal abnormality, a predisposition to a disease or condition, or infection by a pathogen, or for determining identity or heredity.

公开(公告)号：US20020134718A1

公开(公告)日：2002-09-26

申请号：US10093484

申请日：2002-03-11

Inventor： Ole Hindsgaul ,  David C. Schriemer

IPC: B01D015/08

CPC classification number: G01N33/6845 ,  B01J2219/00585 ,  B01J2219/00704 ,  B01J2219/00707 ,  B01J2219/00731 ,  B01J2219/00738 ,  B01J2219/00745 ,  B01J2219/00747 ,  C40B30/08 ,  C40B40/12 ,  C40B40/18 ,  G01N30/466 ,  G01N30/7266 ,  G01N33/538 ,  G01N33/54306 ,  G01N33/54366 ,  G01N33/68 ,  G01N33/6842 ,  G01N33/6848 ,  G01N2030/628 ,  H01J49/00

Abstract: Disclosed are apparatus for screening compound libraries using frontal chromatography in combination with mass spectrometry to identify and rank those members of the library that bind to a target receptor. The apparatus of this invention also permit a compound library to be rapidly screened to determine if any member of the library has an affinity for the target receptor as measured by a pre-selected indicator compound.

公开(公告)号：US20020127609A1

公开(公告)日：2002-09-12

申请号：US10082780

申请日：2002-02-22

Applicant: Maxygen, Inc.

Inventor： S. Christopher Davis ,  Yong Hong Chen ,  Lorraine J. Giver ,  Kurt Vogel

IPC: G01N033/53

CPC classification number: C12Q1/34 ,  H01J49/00

Abstract: Methods of screening for enzyme stereoselectivity that include detecting isotopically labeled products by mass spectrometry are provided. The methods are particularly suitable for screening enzyme libraries for stereoselectivity.

Abstract translation: 提供了通过质谱法检测同位素标记产物的酶立体选择性筛选方法。 该方法特别适用于筛选立体选择性酶库。