公开(公告)号：US09249408B2

公开(公告)日：2016-02-02

申请号：US12734416

申请日：2008-10-31

Applicant: Chang Liu ,  Meng-Lin Tsao ,  Vaughn Smider ,  Peter G. Schultz

Inventor： Chang Liu ,  Meng-Lin Tsao ,  Vaughn Smider ,  Peter G. Schultz

IPC: C40B40/08 ,  C12N15/10 ,  C12N15/67 ,  C40B40/10

CPC classification number: C12N15/1037 ,  C12N15/1058 ,  C12N15/67 ,  C40B40/08 ,  C40B40/10

Abstract: The invention provides methods and compositions for screening polypeptide libraries that include variants comprising unnatural amino acids. In addition, the invention provides vector packaging systems and methods for packaging a nucleic acid in a vector. Compositions of vectors produced by the methods and systems are also provided.

公开(公告)号：US08946128B2

公开(公告)日：2015-02-03

申请号：US13201334

申请日：2010-02-24

Applicant: Geir Åge Løset

Inventor： Geir Åge Løset

IPC: C40B40/08 ,  C12N15/10

CPC classification number: C12N15/1037

Abstract: The present invention provides an alternative scaffold for peptides displayed on filamentous phages through novel fusion proteins primarily originating from pIX. Libraries of filamentous phages can be created from fusion proteins, and a phage display system comprising a phagemid and a helper phage is a part of the invention. An aspect of the invention is a kit containing a phage display system comprising a phagemid that contains a nucleic acid encoding the fusion protein of the invention and a helper phage.

Abstract translation: 本发明通过主要来自pIX的新型融合蛋白提供了在丝状噬菌体上显示的肽的替代性支架。 丝状噬菌体的文库可由融合蛋白产生，包含噬菌粒和辅助噬菌体的噬菌体展示系统是本发明的一部分。 本发明的一个方面是含有噬菌体展示系统的试剂盒，其包含含有编码本发明的融合蛋白的核酸和辅助噬菌体的噬菌粒。

公开(公告)号：US08932879B2

公开(公告)日：2015-01-13

申请号：US12290447

申请日：2008-10-30

Applicant: Mark J. Lim ,  Kenneth J. Rothschild

Inventor： Mark J. Lim ,  Kenneth J. Rothschild

IPC: G01N33/543 ,  C40B50/00 ,  C40B50/14 ,  C40B50/18 ,  C12P21/02 ,  C12N15/10 ,  C40B40/08 ,  C40B50/06 ,  C40B80/00

CPC classification number: C12P21/02 ,  B01J2219/00459 ,  B01J2219/005 ,  B01J2219/00576 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/00722 ,  B01J2219/00725 ,  C12N15/1075 ,  C40B40/08 ,  C40B50/06 ,  C40B80/00

Abstract: Methods are described for phototransferring a compound from a first surface to a second surface. Compounds are described with photocleavable linkers. Compounds attached to a first surface through a photocleavable linker are put in proximity (or contact) with a second surface, and then phototransferred to the second surface upon exposure to electromagnetic radiation. Illuminating the compound with radiation photocleaves the compound from the first surface and transfers the compound to the second surface.

Abstract translation: 描述了将化合物从第一表面转移到第二表面的方法。 用可光洁化的接头描述化合物。 通过光可透洗接头连接到第一表面的化合物与第二表面接近（或接触），然后在暴露于电磁辐射时转移到第二表面。 用辐射照射化合物使化合物从第一表面光刻，并将化合物转移到第二表面。

公开(公告)号：US08795967B2

公开(公告)日：2014-08-05

申请号：US13893273

申请日：2013-05-13

Applicant: Illumina, Inc

Inventor： Mark S. Chee ,  John R. Stuelpnagel ,  Anthony W. Czarnik

IPC: C12Q1/68 ,  C12M1/00 ,  C12M1/34 ,  C12M3/00 ,  C40B40/08 ,  C07H21/02 ,  C07H21/04

CPC classification number: C12Q1/6837 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00459 ,  B01J2219/00466 ,  B01J2219/005 ,  B01J2219/00572 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00619 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00644 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00702 ,  B01J2219/00722 ,  B01L3/5085 ,  B82Y30/00 ,  C12Q1/6816 ,  C12Q1/6825 ,  C12Q1/6834 ,  C40B40/06 ,  C40B60/14 ,  C40B70/00 ,  G01N33/54313 ,  G06K9/6202 ,  Y10S977/924 ,  C12Q2563/155 ,  C12Q2563/179 ,  C12Q2563/107 ,  C12Q2527/137 ,  C12Q2521/313 ,  C12Q2537/143

Abstract: The invention relates to compositions and methods for multiplex decoding of microsphere array sensors.

Abstract translation: 本发明涉及微球阵列传感器的复用解码的组合物和方法。

公开(公告)号：US20130338038A1

公开(公告)日：2013-12-19

申请号：US12340199

申请日：2008-12-19

Applicant: Robert DuBridge ,  David Powers

Inventor： Robert DuBridge ,  David Powers

IPC: C40B30/06 ,  C40B40/02 ,  C40B40/10 ,  C40B50/06 ,  C40B40/08

CPC classification number: C12Q1/6883 ,  C12N15/1037 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2600/16

Abstract: The invention provides methods of making designed and constructed protein (e.g., antibody) libraries and libraries resulting from the same.

公开(公告)号：US20130072748A1

公开(公告)日：2013-03-21

申请号：US13699814

申请日：2011-05-27

Applicant: Samir Hamamah ,  Delphine Haouzi

Inventor： Samir Hamamah ,  Delphine Haouzi

IPC: C12Q1/68 ,  A61B17/435 ,  C40B40/08

CPC classification number: C12Q1/6837 ,  A61B17/435 ,  C12Q1/6883 ,  C12Q2600/158 ,  C40B40/08

Abstract: The present invention relates to a method for assessing the endometrium receptivity of a patient, comprising a step consisting of measuring the expression level of eleven genes in an endometrial biopsy sample obtained from said patient wherein said genes are MFAP5, ANGPTL1, PROK1, NLF2, LAMB3, BCL2L10, CD68, TRPC4, SORCS1, FST and KRT80.

Abstract translation: 本发明涉及一种用于评估患者的子宫内膜接受性的方法，包括测量从所述患者获得的子宫内膜活检样品中11种基因的表达水平的步骤，其中所述基因是MFAP5，ANGPTL1，PROK1，NLF2，LAMB3 ，BCL2L10，CD68，TRPC4，SORCS1，FST和KRT80。

公开(公告)号：US08399384B2

公开(公告)日：2013-03-19

申请号：US13161441

申请日：2011-06-15

Applicant: Robert Charles Ladner

Inventor： Robert Charles Ladner

IPC: C40B40/02 ,  C40B40/08

CPC classification number: C12N15/1037 ,  C07K16/00 ,  C07K16/005 ,  C07K2317/565 ,  C40B40/02 ,  C40B40/08 ,  C40B40/10

Abstract: Focused libraries of vectors or genetic packages that display, display and express, or comprise a member of a diverse family of antibody peptides, polypeptides or proteins and collectively display, display and express, or comprise at least a portion of the focused diversity of the family. The libraries have length and sequence diversities that mimic that found in native human antibodies.

公开(公告)号：US08283294B2

公开(公告)日：2012-10-09

申请号：US12074066

申请日：2008-02-29

Applicant: Jesper Kastrup ,  Lars S. Nielsen ,  Per-Johan Meijer

Inventor： Jesper Kastrup ,  Lars S. Nielsen ,  Per-Johan Meijer

IPC: C40B40/08 ,  C40B50/06

CPC classification number: C12N15/1093 ,  C07K16/005 ,  C07K16/1282 ,  C07K16/2863 ,  C07K2317/24 ,  C07K2317/73

Abstract: The invention relates to a procedure for linking cognate pairs of VH and VL encoding sequences from a population of cells enriched in particular surface antigen markers. The linking procedure involves a multiplex molecular amplification procedure capable of linking nucleotide sequences of interest in connection with the amplification, in particular polymerase chain reaction (multiplex PCR). The method is particularly advantageous for the generation of cognate pair libraries as well as combinatorial libraries of variable region encoding sequences from immunoglobulins. The invention also relates to methods for generation of chimeric human/non-human antibodies and expression libraries generated by such methods.

Abstract translation: 本发明涉及从富集特定表面抗原标记的细胞群中连接VH和VL编码序列的同源对的方法。 连接方法涉及能够连接与扩增有关的核苷酸序列的多重分子扩增方法，特别是聚合酶链式反应（多重PCR）。 该方法特别有利于产生同源对文库以及来自免疫球蛋白的可变区编码序列的组合文库。 本发明还涉及用于产生嵌合人/非人抗体的方法和通过这些方法产生的表达文库。

公开(公告)号：US08252727B2

公开(公告)日：2012-08-28

申请号：US12026412

申请日：2008-02-05

Applicant: Erik Karrer ,  Steven H. Bass ,  Robert Whalen ,  Philip A. Patten

Inventor： Erik Karrer ,  Steven H. Bass ,  Robert Whalen ,  Philip A. Patten

IPC: C07K1/00 ,  C12N15/00 ,  C40B30/00 ,  C40B30/02 ,  C40B30/04 ,  C40B40/08 ,  C40B50/00

CPC classification number: C07K16/1271 ,  C07K16/00 ,  C07K16/1275 ,  C07K2317/24

Abstract: Methods for improving antibodies by a variety of DNA diversification and selection procedures are provided. Improvements include increases in affinity, alterations in specificity and effector function, as well as reduced antigenicity, e.g. humanization. Libraries of recombinant antibody sequences are provided, as are cells expressing members of such libraries. Novel phage display vectors are provided. Methods for the coevolution of an antibody and its cognate antigen are provided. Coevolution is used to evolve HIV envelope proteins with increased antigenicity and broadly neutralizing antibodies that interact therewith. Methods of improving antibodies for use in the detection of biological warfare agents are provided.

Abstract translation: 提供了通过多种DNA多样化和选择程序改进抗体的方法。 改善包括亲和力的增加，特异性和效应子功能的改变，以及降低的抗原性，例如。 人性化。 提供重组抗体序列的文库，以及表达这些文库成员的细胞。 提供了新的噬菌体展示载体。 提供了抗体及其同源抗原共转化方法。 共转化用于发展具有增加的抗原性的HIV包膜蛋白和与之相互作用的广泛中和抗体。 提供了改进用于检测生物战剂的抗体的方法。

公开(公告)号：US20120178646A1

公开(公告)日：2012-07-12

申请号：US13382702

申请日：2010-07-08

Applicant: Herman Pieter Spaink ,  Ronald Petronella Hubertus Dirks

Inventor： Herman Pieter Spaink ,  Ronald Petronella Hubertus Dirks

IPC: C40B30/04 ,  C40B40/08 ,  C07H21/04 ,  C40B60/12

CPC classification number: G01N33/5082 ,  G01N33/5011 ,  G01N33/5023 ,  G01N33/5088

Abstract: Provided is a method and system for screening chemical compounds or compositions, wherein replicating entities are introduced into the yolk of an (un)fertilized egg or embryo. The method may be extended to elucidate the mechanism-of-action of functional chemical compounds or compositions in the same method and system. The method and system may also be employed for identifying marker genes, marker proteins or marker metabolites.

Abstract translation: 提供了用于筛选化合物或组合物的方法和系统，其中将复制实体引入（未）受精卵或胚胎的蛋黄中。 该方法可以扩展以阐明功能性化合物或组合物在相同方法和系统中的作用机理。 该方法和系统也可以用于鉴定标记基因，标记蛋白或标记物代谢物。