公开(公告)号：US20210161003A1

公开(公告)日：2021-05-27

申请号：US17102027

申请日：2020-11-23

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： Amir SADRI ,  Neven NIKOLIC ,  Gabriel CULINCO ,  Thanh-Vi TRAN ,  Nenad KIRCANSKI

IPC: H05K1/02 ,  H05K7/20

Abstract: A thermal management system that include an electronic circuit boards having at least two circuit boards with a space in between and further includes one or more air tubes or conduits. The electronic circuit board and air tubes are configured for drawing air into the space to facilitate cooling of the electronic circuit board concurrent with cooling of a heat sink of a heat pump connected with the electronic circuit board. The system can further include a partition to isolate airflow from the heat sink from airflow through the electronics circuit board, and can further include one or more interface components for maintaining isolation and control of air flow, improving air intake and/or supporting auxiliary components.

公开(公告)号：US20210130880A1

公开(公告)日：2021-05-06

申请号：US17087952

申请日：2020-11-03

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ronald LEBOFSKY

IPC: C12Q1/6834 ,  C12Q1/6855

Abstract: Methods and compositions for attaching cell-specific barcodes without formation of partitions is provided.

公开(公告)号：US10988808B2

公开(公告)日：2021-04-27

申请号：US16802264

申请日：2020-02-26

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Lucas Frenz ,  Jeremy Agresti

IPC: C12Q1/6874 ,  C12Q1/6806

Abstract: Provided herein are compositions and methods for generating phase-shift barcode oligonucleotides for library construction for next-generation sequencing. In some cases, barcode oligonucleotides are attached to particles or beads. Also provided are methods and kits for using the phase-shift barcode oligonucleotides in sequencing assays.

公开(公告)号：US10975422B2

公开(公告)日：2021-04-13

申请号：US16049057

申请日：2018-07-30

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Priyadarshini Gogoi ,  Saedeh Sepehri Javdani ,  Yi Zhou

IPC: C12Q1/68 ,  G01N33/487 ,  G01N33/574 ,  C12Q1/6841 ,  C12Q1/6844 ,  C12Q1/686 ,  G01N27/447

Abstract: A system and method for capturing and analyzing a set of cells, comprising: an array including a set of parallel pores, each pore including a chamber including a chamber inlet and a chamber outlet, and configured to hold a single cell, and a pore channel fluidly connected to the chamber outlet; an inlet channel fluidly connected to each chamber inlet of the set of parallel pores; an outlet channel fluidly connected to each pore channel of the set of parallel pores; a set of electrophoresis channels fluidly coupled to the outlet channel, configured to receive a sieving matrix for electrophoretic separation; and a set of electrodes including a first electrode and a second electrode, wherein the set of electrodes is configured to provide an electric field that facilitates electrophoretic analysis of the set of cells.

公开(公告)号：US10962563B2

公开(公告)日：2021-03-30

申请号：US16246298

申请日：2019-01-11

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Aaron Weber ,  Tony Hung ,  Sepehr Kiani

IPC: G01P5/18 ,  G01P5/20 ,  G01P5/26

Abstract: Methods and systems are provided for measuring a velocity of a droplet passing through a microfluidic channel.

公开(公告)号：US20210088519A1

公开(公告)日：2021-03-25

申请号：US17081220

申请日：2020-10-27

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Darren R. Link ,  Michael L. Samuels

IPC: G01N33/573 ,  B01F5/06 ,  B01J19/00 ,  B01F13/00 ,  B01L3/00 ,  B01F3/08 ,  C40B40/04 ,  C40B50/08 ,  C40B60/10 ,  G01N33/50 ,  C12Q1/25 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/6827 ,  G01N33/542 ,  G01N33/58

Abstract: The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the subset. The amount of target can be determined based on the detected number.

公开(公告)号：US20210071173A1

公开(公告)日：2021-03-11

申请号：US17102111

申请日：2020-11-23

Applicant: Bio-Rad Laboratories, Inc. ,  Bio-Rad Europe GmbH

Inventor： Ronald LEBOFSKY ,  Jeremy AGRESTI

IPC: C12N15/10

Abstract: Methods and compositions are provided herein for preparing high-throughput cDNA sequencing libraries.

公开(公告)号：US20210069692A1

公开(公告)日：2021-03-11

申请号：US17012108

申请日：2020-09-04

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： CHRISTOPHER BELISLE ,  XUEMEI HE ,  HONG CHEN ,  YUEPING XU ,  JIALI LIAO

IPC: B01J41/14 ,  B01J41/07 ,  B01J47/02 ,  B01J47/014 ,  C07K1/18 ,  C12N9/88

Abstract: Chromatography resins having anionic exchange-hydrophobic mixed mode ligands and methods of using such resins are provided.

公开(公告)号：US10941445B2

公开(公告)日：2021-03-09

申请号：US15926341

申请日：2018-03-20

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Tal Raz ,  Pascaline Mary

IPC: C12Q1/6876 ,  C12Q1/6869 ,  C12Q1/6848 ,  C12Q1/6806 ,  C12Q1/682

Abstract: The present invention relates to methods of target nucleic acid amplification and uses thereof in methods of sequencing, or other down-stream applications such a genotyping or cloning. More specifically, the methods relate to methods of target nucleic acid amplification through the use of a universal hairpin primer.

公开(公告)号：US10927407B2

公开(公告)日：2021-02-23

申请号：US16022360

申请日：2018-06-28

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Darren Roy Link

IPC: B01L3/00 ,  C12Q1/6848 ,  G01N1/38 ,  C12Q1/686 ,  B01J19/00 ,  B01F13/00 ,  B03C5/00 ,  B03C5/02 ,  B01F3/08 ,  C12Q1/00 ,  C12Q1/6806 ,  C12Q1/6844 ,  C12Q1/6874 ,  G01N35/08 ,  G01N15/14 ,  C12N15/10 ,  C12Q1/6827 ,  B01L7/00 ,  B01L9/00 ,  G01N35/00

Abstract: The invention generally relates to assemblies for displacing droplets from a vessel that facilitate the collection and transfer of the droplets while minimizing sample loss. In certain aspects, the assembly includes at least one droplet formation module, in which the module is configured to form droplets surrounded by an immiscible fluid. The assembly also includes at least one chamber including an outlet, in which the chamber is configured to receive droplets and an immiscible fluid, and in which the outlet is configured to receive substantially only droplets. The assembly further includes a channel, configured such that the droplet formation module and the chamber are in fluid communication with each other via the channel. In other aspects, the assembly includes a plurality of hollow members, in which the hollow members are channels and in which the members are configured to interact with a vessel.