公开(公告)号：US20190249244A1

公开(公告)日：2019-08-15

申请号：US16396284

申请日：2019-04-26

Applicant: GENIA TECHNOLOGIES, INC.

Inventor： Randall DAVIS ,  Roger CHEN

IPC: C12Q1/6869 ,  G01N33/487 ,  C12N15/11 ,  G01N33/543

CPC classification number: C12Q1/6869 ,  B01J2219/00313 ,  B01J2219/00653 ,  B01J2219/00734 ,  C12N15/111 ,  C12N2310/11 ,  C12N2310/16 ,  C12N2310/3517 ,  C12N2320/10 ,  C12N2320/32 ,  C12Q2565/607 ,  C12Q2565/631 ,  G01N33/48721 ,  G01N33/5432

Abstract: The present disclosure provides biochips and methods for making biochips. A biochip can comprise a nanopore in a membrane (e.g., lipid bilayer) adjacent or in proximity to an electrode. Methods are described for forming the membrane and insert-ing the nanopore into the membrane. The biochips and methods can be used for nucleic acid (e.g., DNA) sequencing. The present disclosure also describes methods for detecting, sorting, and binning molecules (e.g., proteins) using biochips.

公开(公告)号：US20190212296A1

公开(公告)日：2019-07-11

申请号：US16238451

申请日：2019-01-02

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen ,  Hui Tian ,  J. William Maney, JR.

IPC: G01N27/447 ,  C12Q1/6869 ,  B01L3/00 ,  G01N33/487

CPC classification number: G01N27/44791 ,  B01L3/50273 ,  B01L2400/0421 ,  C12Q1/6869 ,  G01N27/44743 ,  G01N33/48721 ,  C12Q2565/607 ,  C12Q2565/631

Abstract: A method of analyzing a molecule in a nanopore is disclosed. A voltage is applied across a nanopore that is inserted in a membrane by coupling the nanopore to a voltage source. The nanopore is decoupled from the voltage source. After the decoupling, a rate of decay of the voltage across the nanopore is determined. A molecule in the nanopore is distinguished from other possible molecules based on the determined rate of decay of the voltage across the nanopore.

公开(公告)号：US10316360B2

公开(公告)日：2019-06-11

申请号：US15809725

申请日：2017-11-10

Applicant: GENIA TECHNOLOGIES, INC.

Inventor： Randall Davis ,  Roger Chen

IPC: C12Q1/6869 ,  G01N33/487 ,  G01N33/543 ,  C12N15/11

Abstract: Provided is a method for detecting a target molecule. The method includes providing a chip, the chip including a nanopore in a membrane that is disposed adjacent to or in proximity to a sensing electrode. A nucleic acid molecule is then directed through the nanopore, the nucleic acid molecule being associated with a reporter molecule. The nucleic acid molecule also includes an address region and a probe region, the reporter molecule being associated with the nucleic acid molecule at the probe region. The reporter molecule is also coupled to a target molecule. While the nucleic acid molecule is directed through the nanopore, the address region can be sequenced to determine a nucleic acid sequence of the address region. The target molecule can then be identified, with the aid of a computer processor, based upon the nucleic acid sequence of the address region.

公开(公告)号：US20190079067A1

公开(公告)日：2019-03-14

申请号：US16146053

申请日：2018-09-28

Applicant: GENIA TECHNOLOGIES, INC.

Inventor： Timothy Craig ,  Corissa Harris ,  Matt Dipetro ,  Mark Ambroso ,  Yaozhong Zou ,  Marshall Porter ,  Issa Delkaninia ,  Alexxa Noble ,  James Fairman ,  Wiseley Wu ,  Kapil Bajaj ,  Giovanni Bellesia ,  Seong-Ho Shin

IPC: G01N33/487 ,  C07K14/31

Abstract: Described herein are nanopore protein conjugates that can be used in DNA sequencing reactions. The nanopore protein conjugates includes a nanopore protein monomer that is joined to a DNA binding domain. The nanopore protein monomer is available to oligomerize with other nanopore protein monomers, while the DNA binding domain is available to bind to a template DNA strand. In certain examples, the nanopore protein monomer is an alpha-hemolysin monomer or variant thereof and the DNA binding domain is an Sso7d protein or variant thereof, such as an Sso7d-like protein. Also provided are nanopore protein assemblies incorporating the nanopore protein conjugates, along with methods of using the nanopore protein assemblies in sequencing reactions.

公开(公告)号：US20190079049A1

公开(公告)日：2019-03-14

申请号：US16157019

申请日：2018-10-10

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen

IPC: G01N27/447 ,  G01N33/487 ,  G01N27/327

CPC classification number: G01N27/44791 ,  G01N27/3278 ,  G01N27/4473 ,  G01N27/44743 ,  G01N33/48721

Abstract: A method of identifying a molecule is disclosed. A molecule is drawn to a nanopore by applying a first voltage signal to a pair of electrodes during a first period, wherein the first voltage signal causes a first ionic current through the nanopore that is indicative of a property of a portion of the molecule proximate to the nanopore. The molecule is released from the nanopore by applying a second voltage signal to the pair of electrodes during a second period, wherein the second voltage signal causes a second ionic current through the nanopore. The first period and the second period are determined based at least in part on a net ionic current through the nanopore comprising the first ionic current and the second ionic current.

公开(公告)号：US10215731B2

公开(公告)日：2019-02-26

申请号：US15371106

申请日：2016-12-06

Applicant: Genia Technologies, Inc.

Inventor： Roger J. A. Chen ,  Hui Tian ,  J. William Maney, Jr.

IPC: G01N27/447 ,  B01L3/00 ,  C12Q1/6869 ,  G01N33/487

Abstract: A method of analyzing a molecule in a nanopore is disclosed. A voltage is applied across a nanopore that is inserted in a membrane by coupling the nanopore to a voltage source. The nanopore is decoupled from the voltage source. After the decoupling, a rate of decay of the voltage across the nanopore is determined. A molecule in the nanopore is distinguished from other possible molecules based on the determined rate of decay of the voltage across the nanopore.

公开(公告)号：US20180362594A1

公开(公告)日：2018-12-20

申请号：US15762092

申请日：2016-09-20

Applicant: GENIA TECHNOLOGIES, INC.

Inventor： Cynthia CECH ,  Tim CRAIG ,  Christos TZITZILONIS ,  Alexander YANG ,  Liv JENSEN ,  Charlotte YANG ,  Corissa HARRIS

IPC: C07K14/245 ,  C12N9/12 ,  C12Q1/6874

CPC classification number: C07K14/245 ,  C07K2319/00 ,  C12N9/1252 ,  C12Q1/68 ,  C12Q1/6874 ,  C12Y207/07007 ,  G01N33/6803 ,  C12Q2525/197 ,  C12Q2525/203 ,  C12Q2565/631

Abstract: The present disclosure provides variant OmpG polypeptides, compositions comprising the OmpG variant polypeptides, and methods for using the variant OmpG polypeptides as nanopores for determining the sequence of single stranded nucleic acids. The variant OmpG nanopores reduce the ionic current noise versus the parental OmpG polypeptide from which they are derived and thereby enable sequencing of polynucleotides with single nucleotide resolution. The reduced ionic current noise also provides for the use of these OmpG nanopore variants in other single molecule sensing applications, e.g., protein sequencing.

公开(公告)号：US10126262B2

公开(公告)日：2018-11-13

申请号：US14864395

申请日：2015-09-24

Applicant: Genia Technologies, Inc.

Inventor： Bill Maney ,  Hui Tian ,  Santiago Fernandez-Gomez

IPC: G01N27/26 ,  B82Y35/00 ,  G01N33/487

Abstract: A nanopore measurement circuit is disclosed. The nanopore measurement circuit includes a nanopore electrode, a first analog memory and a second analog memory. The nanopore measurement circuit also includes a switch network that selectively connects the nanopore electrode to at least one of the first analog and the second analog memory.

公开(公告)号：US20180299400A1

公开(公告)日：2018-10-18

申请号：US15768215

申请日：2016-10-21

Applicant: Genia Technologies, Inc.

Inventor： John Foster ,  Steven Henck

IPC: G01N27/327 ,  C12Q1/6869 ,  G01N33/487 ,  G01N27/40

Abstract: A method of sequencing a DNA sample is disclosed. A nanopore-based sequencing device is provided. The nanopore-based sequencing device includes a conductive layer. The device further includes a working electrode disposed above the conductive layer. The device further includes a side wall disposed above the working electrode, wherein the side wall and the working electrode form a well in which an electrolyte may be contained, and wherein at least an upper portion of the side wall comprises a hydrophobic portion formed by a fluoropolymer material. The DNA sample is sequenced using the nanopore-based sequencing device.

公开(公告)号：US10006899B2

公开(公告)日：2018-06-26

申请号：US14225263

申请日：2014-03-25

Applicant: Genia Technologies, Inc.

Inventor： Hui Tian

IPC: C12Q1/68 ,  G01N33/487

CPC classification number: G01N33/48721

Abstract: A nanopore based sequencing chip is disclosed. The sequencing chip comprises a first portion made from a first wafer. The first portion includes an array of nanopore cells. The first portion further includes a measurement circuit connected to one or more nanopore cells, the measurement circuit producing an output measurement signal. The first portion further includes one or more vias transmitting the output measurement signal. The sequencing chip further includes a second portion made from a second wafer, the second portion comprising one or more corresponding vias receiving the output measurement signal.