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公开(公告)号：US20100035315A1
公开(公告)日：2010-02-11
申请号：US12514215
申请日：2007-11-13
申请人： Rainer Stürmer , Bernhard Hauer , Thomas Friedrich , Kurt Faber , Melanie Hall
发明人： Rainer Stürmer , Bernhard Hauer , Thomas Friedrich , Kurt Faber , Melanie Hall
IPC分类号： C12P7/26
CPC分类号： C12P7/24 , C12P7/26 , C12P7/40 , C12P7/46 , C12P7/62 , C12P13/008 , C12P17/10 , C12P41/002
摘要： A method for enzymatic preparation of compounds of the general formula (2) from unsaturated alkene derivatives of the general formula (1) by reducing a compound of the formula (1) in the presence of a reductase, comprising at least one of the polypeptide sequences SEQ ID NO: 1, 2 or 3 or having a functionally equivalent polypeptide sequence which is at least 80% identical to SEQ ID NO: 1, 2 or 3.
摘要翻译： 一种通过在还原酶的存在下还原式（1）的化合物，从通式（1）的不饱和烯烃衍生物中酶制备通式（2）的化合物的方法，所述方法包括至少一种多肽序列 具有与SEQ ID NO：1,2或3至少80％相同的功能等同多肽序列的SEQ ID NO：1,2或3。
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82.发明申请PROCESS FOR THE PREPARATION OF OPTICALLY ACTIVE 5-SUBSTITUTED 2-OXAZOLIDINONES FROM RACEMIC EPOXIDES AND CYANATE EMPLOYING A HALOHYDRIN DEHALOGENASE 失效用于制备光化活性的5-取代的2-取代的二氧杂环己烷的方法来自环氧化物和使用氢化物脱氢酶的氰酸酯公开(公告)号：US20090042261A1
公开(公告)日：2009-02-12
申请号：US12279786
申请日：2007-02-27
申请人： Bernhard Hauer
发明人： Bernhard Hauer
IPC分类号： C12P17/14
CPC分类号： C12P17/14 , C12P41/006
摘要： A process for the production of an optically enriched oxazolidinone of the formula (2a) or (2b), by reacting an epoxide of the formula (1) with cyanate in the presence of halo-hydrin dehalogenase.
摘要翻译： 通过式（1）的环氧化物与卤代 - 卤素脱卤素酶的存在下的氰酸酯反应制备式（2a）或（2b）的光学富集的恶唑烷酮的方法。
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83.发明授权Method for analyzing enzyme-catalyzed reactions using MALDI-TOF mass spectrometry 失效使用MALDI-TOF质谱法分析酶催化反应的方法公开(公告)号：US07445885B2
公开(公告)日：2008-11-04
申请号：US10296381
申请日：2001-06-06
CPC分类号： H01J49/04
摘要： A process is described for analyzing enzyme-catalyzed conversions of nonpolymeric substrates to nonpolymeric products with the aid of MALDI-TOF mass spectrometry, preferably in the presence of an internal standard on a specific carrier material.
摘要翻译： 描述了一种方法，用于借助MALDI-TOF质谱法，优选在特定载体材料上存在内标物的情况下分析非聚合物底物对非聚合物产物的酶催化转化。
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公开(公告)号：US20080009046A1
公开(公告)日：2008-01-10
申请号：US11667721
申请日：2005-11-15
IPC分类号： C12P7/16
摘要： The present invention relates to enzymic processes for preparing S-butan-2-ol; and to enzymes for carrying out said processes; to nucleic acid sequences coding for said enzymes, to expression cassettes, vectors and recombinant hosts containing said nucleic acid sequences.
摘要翻译： 本发明涉及制备S-丁-2-醇的酶法; 和用于进行所述方法的酶; 编码所述酶的核酸序列，含有所述核酸序列的表达盒，载体和重组宿主。
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公开(公告)号：US07314739B2
公开(公告)日：2008-01-01
申请号：US10493210
申请日：2002-10-17
CPC分类号： C12N9/20
摘要： The invention relates to a lipase variant which can be prepared by carrying out, on the amino acid sequence of a starting lipase selected from the lipase homologous family I.1 or I.2, at least one amino acid substitution in those positions which correspond to the positions 17, 29, 30, 52, 86, 117, 122, 160, 163, 167, 265, 266, 286, 289 in the prototype lipase sequence SEQ ID NO: 1.
摘要翻译： 本发明涉及脂酶变体，其可通过在选自脂肪酶同源家族I.1或I.2的起始脂肪酶的氨基酸序列上进行至少一个氨基酸取代而制备， 原型脂肪酶序列SEQ ID NO：1中的位置17,29,30,25,66,117,122,160,163,167,265,266,286,289。
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86.发明申请Process for an Enzymatic Oxygenation by Direct Electrochemical Regeneration of the Fad-Dependent Monooxygenase 审中-公开通过直接电化学再生法自由基单氧化酶进行酶促氧化的方法公开(公告)号：US20070231871A1
公开(公告)日：2007-10-04
申请号：US11596301
申请日：2005-05-11
IPC分类号： C12P1/00
CPC分类号： C12P1/00 , C12N9/0071 , C12P7/00
摘要： Processes comprising oxygenating an educt to form a product in the presence of an FAD-dependent monooxygenase, wherein the FAD-dependent monooxygenase is regenerated by direct electrochemical reduction are described.
摘要翻译： 描述了通过在FAD依赖性单加氧酶存在下使排气物氧化以形成产物的方法，其中通过直接电化学还原再生FAD依赖性单加氧酶。
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87.发明申请Methods for the production of 3-methylamino-1-(thiene-2-yl)-propane-1-ol 有权3-甲基氨基-1-（噻吩-2-基） - 丙-1-醇的制备方法公开(公告)号：US20070083055A1
公开(公告)日：2007-04-12
申请号：US10573130
申请日：2004-09-30
IPC分类号： C07D333/22 , C12P17/00 , C12N9/02 , C12N1/21 , C12N1/18
CPC分类号： C07D333/16
摘要： The present invention relates to enzymic and nonenzymic processes for preparing 3-methylamino-1-(thien-2-yl)propan-1-ol; as well as to enzymes for implementing these processes; and to nucleic acid sequences encoding these enzymes, to expression cassettes containing these nucleic acid sequences, to vectors and to recombinant hosts.
摘要翻译： 本发明涉及制备3-甲基氨基-1-（噻吩-2-基）丙-1-醇的酶和非酶法; 以及用于实施这些过程的酶; 以及编码这些酶的核酸序列，含有这些核酸序列的表达盒，载体和重组宿主。
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公开(公告)号：US07189545B2
公开(公告)日：2007-03-13
申请号：US10488007
申请日：2002-08-16
申请人： Michael Breuer , Bernhard Hauer , Thomas Friedrich
发明人： Michael Breuer , Bernhard Hauer , Thomas Friedrich
CPC分类号： C12N9/88 , C12P41/002
摘要： A polypeptide with pyruvate decarboxylase activity, derived from the pyruvate decarboxylase from Zymomonas mobilis by substitution of an amino acid in position 553.
摘要翻译： 具有丙酮酸脱羧酶活性的多肽，通过用位置553的氨基酸取代来自运动发酵单胞菌的丙酮酸脱羧酶。
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89.发明授权Method for producing chiral carboxylic acids from nitriles with the assistance of a nitrilase or microoganisms which contain a gene for the nitrilase 有权在腈水解酶或含有腈水解酶基因的微生物的帮助下，由腈生产手性羧酸的方法公开(公告)号：US06869783B1
公开(公告)日：2005-03-22
申请号：US09806876
申请日：1999-10-13
IPC分类号： C12N15/09 , C07H21/00 , C12N1/21 , C12N9/78 , C12N15/55 , C12P7/40 , C12P7/42 , C12P41/00 , C12R1/05 , C12P7/62
CPC分类号： C07H21/00 , C12N9/78 , C12P7/42 , C12P41/006
摘要： The invention relates to nucleic acid sequences which code for a polypeptide having nitrilase activity, to nucleic acid constructs comprising the nucleic acid sequences, and to vectors comprising the nucleic acid sequences or the nucleic acid constructs. The invention further relates to amino acid sequences which are encoded by the nucleic acid sequences, and to microorganisms comprising the nucleic acid sequences, the nucleic acid constructs or vectors comprising the nucleic acid sequences or the nucleic acid constructs.The invention additionally relates to a process for preparing chiral carboxylic acids from the racemic nitriles.
摘要翻译： 本发明涉及编码具有腈水解酶活性的多肽的核酸序列，包含核酸序列的核酸构建体以及包含核酸序列或核酸构建体的载体。 本发明还涉及由核酸序列编码的氨基酸序列，以及包含核酸序列，核酸构建体或包含核酸序列或核酸构建体的载体的微生物。本发明还涉及一种方法 用于从外消旋腈制备手性羧酸。
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公开(公告)号：US20050048484A1
公开(公告)日：2005-03-03
申请号：US10398178
申请日：2001-10-16
申请人： Bernhard Hauer , Rolf Schmid , Rainer Merkl , Francesca Blasco
发明人： Bernhard Hauer , Rolf Schmid , Rainer Merkl , Francesca Blasco
IPC分类号： C12N15/09 , C12M1/40 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/02 , C12N15/53 , C12P1/04 , C12P7/00 , C12R1/01 , C12Q1/68 , C07H21/04 , C12N15/74
CPC分类号： C12N9/0077 , C12P1/04
摘要： The invention relates to novel cytochrome P450 monooxygenases from thermophilic bacteria, in particular the genus Thermus sp., to nucleotide sequences encoding them, to the recombinant production of these monooxygenases and to their use for the microbiological oxidation of organic compounds.
摘要翻译： 本发明涉及嗜热细菌，特别是栖热菌属的新型细胞色素P450单加氧酶与编码它们的核苷酸序列，重组生产这些单加氧酶及其用于微生物氧化有机化合物的新型细胞色素P450单加氧酶。
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