公开(公告)号：US11635383B2

公开(公告)日：2023-04-25

申请号：US17211154

申请日：2021-03-24

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Matthew Beaudet ,  Jill Hendrickson ,  David Hagen ,  Rich Meyer

IPC: G01N21/64 ,  B01L7/00 ,  G01N15/14 ,  G01N35/00 ,  B01L3/00

Abstract: Devices and methods for measuring the quantity of multiple analytes in a sample can include a device designed such that each of the analyte sensing elements is configured to measure the quantity of a predetermined analyte and machine executable instructions configured to select the proper analyte sensing element corresponding to the analyte to be measured.

公开(公告)号：US20230124451A1

公开(公告)日：2023-04-20

申请号：US18076986

申请日：2022-12-07

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Khairuzzaman Bashar MULLAH ,  Brian EVANS ,  Scott C. BENSON ,  Chu-An CHANG ,  Xiongwei YAN

IPC: C12Q1/6818 ,  C12Q1/686

Abstract: Disclosed is a probe for use in biological assays. The probe includes a fluorescent dye bound to a quencher compound through an oligonucleotide linker. Also disclosed are methods of using the probe, such as for a polymerase chain reaction (PCR), such as in a quantitative PCR reaction (qPCR), as well as kits including the probe.

公开(公告)号：US20230119629A1

公开(公告)日：2023-04-20

申请号：US18069264

申请日：2022-12-21

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Glen Davis

IPC: G01F25/17 ,  G01F15/00 ,  G01N29/22

Abstract: Provided are systems and methods for line volume calibration, and measurement of fluid samples delivered to an interrogation point. In various embodiments, a known fluid volume comprising a sample line fluid and a secondary fluid is delivered to a fluid boundary sensor. The fluid boundary sensor assists in determining the position of the boundaries between the various fluids, and the positions of these boundaries are used to determine the sample line fluid volume.

公开(公告)号：USD983813S1

公开(公告)日：2023-04-18

申请号：US29758178

申请日：2020-11-12

Applicant: LIFE TECHNOLOGIES CORPORATION

Designer： Laurel Stone ,  Emily Zink ,  Sean Zimmerman ,  Nicholas Diliani

公开(公告)号：US20230101252A1

公开(公告)日：2023-03-30

申请号：US18077404

申请日：2022-12-08

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： John NOBILE ,  George Thomas ROTH ,  Todd REARICK ,  Jonathan SCHULTZ ,  Jonathan M. ROTHBERG ,  David MARRAN

IPC: G01N27/30 ,  B01L3/00 ,  G01N27/414

Abstract: An apparatus includes a reaction vessels coupled to an electronic sensor for monitoring a reaction product in the reaction vessel; a fluidics system for sequentially delivering a plurality of reagents to the reaction vessel, the fluidics system including a plurality of reagent reservoirs in fluidic communication via a plurality of flow paths with a fluidics circuit and to a common passage in fluidic communication between the fluidics circuit and the reaction vessel, a solution reservoir in fluidic communication with the common passage via a branch passage connected with the common passage at a junction between the fluidics circuit and the reaction vessel; and an electrode in contact with a solution within the branch passage, the electrode being in electrical communication with the reaction vessel through fluid extending from the branch passage and through the common passage, the electronic sensor generating an output signal depending on a voltage of the electrode.

公开(公告)号：US20230093549A1

公开(公告)日：2023-03-23

申请号：US18060728

申请日：2022-12-01

Applicant: Life Technologies Corporation

Inventor： Clinton C. Staheli ,  Brandon M. Knudsen ,  Trevor G. Williams ,  Steven R. Kjar

IPC: B01F27/21 ,  C12M1/00 ,  C12M1/06 ,  B01F27/07 ,  B01F27/88 ,  B01F27/191 ,  B01F27/054 ,  B01F27/072 ,  B01F27/906 ,  B01F27/1125 ,  B01F27/2111 ,  B01F35/41 ,  B01F35/513

Abstract: A method of mixing a fluid includes at least partially unfolding a collapsible bag bounding a compartment, the collapsible bag containing in the compartment at least a portion of an elongated drive line or drive shaft and an impeller secured to the drive line or drive shaft, the impeller including a plurality of impeller blades that are pivotable relative to the drive line or drive shaft, at least one of the plurality of impeller blades being in a collapsed position. A fluid is delivered into the compartment of the collapsible bag. The drive line or drive shaft is then rotated so as to rotate the impeller within the compartment and mix the fluid therein, the at least one of the plurality of impeller blades pivoting from the collapsed position to an expanded position as the impeller is rotated within the compartment.

公开(公告)号：US20230085832A1

公开(公告)日：2023-03-23

申请号：US17936947

申请日：2022-09-30

Applicant: Life Technologies Corporation

Inventor： Kyle GEE ,  Kathleen KIHN ,  Yi-Zhen HU ,  Yexin WU ,  Kathleen FREE

IPC: G01N21/64 ,  C07D311/82 ,  C07D209/10 ,  C07F15/00 ,  C07F9/24

Abstract: Provided herein are compounds, compositions, kits, uses, and methods for assessing the viability of cells and/or quantifying the amount of live and dead cells in a mixture using differential stains. In some embodiments, the compounds are used in culture media or can function independently of fixation and/or permeabilization. In some embodiments, the compounds comprise a platinum atom. In some embodiments, the compounds comprise a nitrogen mustard moiety.

公开(公告)号：US11608491B2

公开(公告)日：2023-03-21

申请号：US16798193

申请日：2020-02-21

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Xin Yu ,  Xavier de Mollerat du Jeu ,  Chao Yan Liu ,  Jian Liu ,  Jonathan Zmuda

IPC: C12N15/86 ,  C12N5/10 ,  C12N7/00 ,  C07K14/005 ,  C12N15/64 ,  C12N1/06

Abstract: The instant technology relates to a production system to produce AAV vectors in a serum free suspension platform and at high titers. This technology uses reagents comprising media, cells, transfection reagent, AAV enhancer, and a lysis buffer, each of which is designed to provide maximal AAV production from suspension culture of mammalian cells, e.g. HEK293 cells. With this new system we are able to deliver up to about 2×1011 viral genomes per milliliter (vg/mL) of unconcentrated AAV vectors.

公开(公告)号：US11598434B2

公开(公告)日：2023-03-07

申请号：US17147586

申请日：2021-01-13

Applicant: Life Technologies Corporation

Inventor： Charles R. Rhodes ,  Jonathan O. Schultz

IPC: F16K15/14

Abstract: A check valve assembly includes a first port fitting and a second port fitting with a base secured therebetween. The base has an annular sleeve encircling a seat, the seat having a central mounting hole and one or more flow channels passing therethrough. An umbrella valve includes a flexible sealing disk having an outer surface and an opposing inner surface, a mounting stem extending from the inner surface and projecting into the mounting hole of the base, the sealing disk being movable between a first position wherein at least a portion of the sealing disk sits on the seat so as to cover the one or more flow channels and a second position wherein the sealing disk is resiliently flexed so as to at least partially uncover the one or more flow channels. A retention plate is disposed between first port fitting and the seat of the base so that the retention plate sits against the outer surface of the sealing disk.

公开(公告)号：US11573118B2

公开(公告)日：2023-02-07

申请号：US16892502

申请日：2020-06-04

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Glen Davis

IPC: G01N29/22 ,  G01F25/17 ,  G01F15/00

Abstract: Provided are systems and methods for line volume calibration, and measurement of fluid samples delivered to an interrogation point. In various embodiments, a known fluid volume comprising a sample line fluid and a secondary fluid is delivered to a fluid boundary sensor. The fluid boundary sensor assists in determining the position of the boundaries between the various fluids, and the positions of these boundaries are used to determine the sample line fluid volume.