公开(公告)号：US20210206816A1

公开(公告)日：2021-07-08

申请号：US16670125

申请日：2019-10-31

申请人： Board of Trustees of Michigan State University

发明人： Cheryl A. Kerfeld ,  Cesar R. Gonzalez

IPC分类号： C07K14/405 ,  C10L1/02 ,  C12N1/32 ,  C12N9/14 ,  C12P5/00 ,  C12N15/82 ,  C12P7/16 ,  C12P7/06 ,  C12P7/24 ,  C07K14/195

摘要： A fusion chimeric protein is described herein that can assemble a functional carboxysome core, which is able to fix carbon by taking atmospheric carbon dioxide and converting it into useful carbon-containing compounds such as 3-phosphoglycerate (3-PGA).

公开(公告)号：US20210040440A1

公开(公告)日：2021-02-11

申请号：US16967834

申请日：2019-02-06

申请人： White Dog Labs, Inc.

发明人： Bryan P. TRACY ,  Shawn William JONES ,  Alon KARPOL ,  Hadar GILARY ,  Aharon M. EYAL

IPC分类号： C12N1/20 ,  C12N1/32

摘要： A selection method including providing a microorganism preparation including a variety of microorganisms and optionally water; providing an organic liquid including at least 70% by weight hydrophobic solvent; forming a multiple phase medium including a selected amount of the microorganism preparation and a selected amount of the organic liquid; and maintaining the multiple phase medium at a selected temperature for selected duration, whereby a treated microorganism preparation is formed.

公开(公告)号：US10889842B2

公开(公告)日：2021-01-12

申请号：US14599383

申请日：2015-01-16

申请人： CALYSTA, INC.

发明人： Renee M. Saville ,  Joshua A. Silverman ,  Eric G. Luning ,  Brandon D. Doss ,  Lorraine Joan Giver ,  Sol M. Resnick ,  Drew D. Regitsky

IPC分类号： C12N1/04 ,  C12N1/32 ,  C12P13/08 ,  C12P13/12 ,  C12N15/52 ,  C12N1/30

摘要： The present disclosure relates to recombinant microorganisms engineered for enhance production of a desired amino acid, as well as related biomass, and compositions which are useful, inter alia, as animal feed ingredients. The present invention also provides related methods.

公开(公告)号：US10717964B2

公开(公告)日：2020-07-21

申请号：US16042257

申请日：2018-07-23

申请人： University of Delaware

发明人： Eleftherios T. Papoutsakis ,  Sergios Nicolaou ,  Alan Fast ,  Vasiliki Falara ,  Robert Kyle Bennett ,  William Brian Whitaker ,  Nicholas Richard Sandoval ,  Jacqueline Gonzalez ,  Maciek Antoniewicz

IPC分类号： C12N1/32 ,  C12P7/16 ,  C12P7/40 ,  C12P7/42 ,  C12P7/44 ,  C12P7/46 ,  C12P7/62 ,  C12N9/04 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12N15/70

摘要： A non-naturally occurring microbe capable of growing in a medium comprising methanol is provided. The methanol contributes to a significant percentage (e.g., at least 40%) of the carbon source for the non-naturally occurring microbe, which expresses heterologous methanol dehydrogenase (MDH) and heterologous ribulose monophosphate (RuMP) pathway enzymes. Methods for producing liquid fuels and chemicals by the non-naturally occurring microbe and methods for preparing the non-naturally occurring microbe are also provided.

公开(公告)号：US10533999B2

公开(公告)日：2020-01-14

申请号：US15038554

申请日：2014-12-08

申请人： BIOMERIEUX

发明人： Florence Bettsworth ,  Jérôme Martinez

IPC分类号： C12N1/32 ,  G01N33/573 ,  C12N9/06 ,  C12N9/96 ,  C12Q1/04 ,  C12Q1/32

摘要： A process for stabilizing glutamate dehydrogenase (GDH) from a bacterium of the Clostridium genus, in an aqueous solution, in order to maintain the antigenic properties thereof, includes the step of mixing the glutamate dehydrogenase and a stabilizing composition which is a carboxylic acid having a carbon-based chain of at least 3 carbon atoms and comprising at least 2 —COOH groups, or a salt thereof. GDH compositions thus stabilized and a method of detecting the presence of bacteria of the Clostridium genus are also disclosed.

公开(公告)号：US20190024040A1

公开(公告)日：2019-01-24

申请号：US16070318

申请日：2017-01-13

申请人： University of Delaware

发明人： Eleftherios T. Papoutsakis ,  William Brian Whitaker ,  Robert Kyle Bennett

IPC分类号： C12N1/32 ,  C12N15/52 ,  C12N9/04 ,  C12R1/15 ,  C12R1/19

摘要： The present invention provides a non-naturally occurring microbe capable of growing in a medium comprising methanol, comprising a heterologous polynucleotide encoding a heterologous methanol dehydrogenase (MDH) derived from a Corynebacterium organism (Cor), wherein the MDH is expressed in the microbe, and wherein the MDH exhibits a Km of no more than 3 mM for methanol. Also provided are uses of the non-naturally occurring microbe for oxidizing methanol and producing a metabolite as well as the preparation of the non-naturally occurring microbe.

公开(公告)号：US10138435B2

公开(公告)日：2018-11-27

申请号：US15173335

申请日：2016-06-03

申请人： Corbion Biotech, Inc.

发明人： Donald E. Trimbur ,  Chung-Soon Im ,  Harrison F. Dillon ,  Anthony G. Day ,  Scott Franklin ,  Anna Coragliotti

IPC分类号： A61K48/00 ,  C12P21/06 ,  C12N1/12 ,  C07H21/04 ,  C10L1/02 ,  C12N1/32 ,  C12N9/04 ,  C12N9/02 ,  C12N9/12 ,  C12N9/16 ,  C12N9/20 ,  C12N9/26 ,  C12N9/88 ,  C12P5/02 ,  C12P7/64 ,  C11C3/00 ,  C12N15/79 ,  C10L1/06 ,  C12N9/14

摘要： The invention provides methods of manufacturing alkanes from triglyceride oils produced through fermentation of oil-bearing microbes. The processes provided herein can utilize a variety of carbohydrate feedstocks including cane bagasse, sugar beet pulp, corn stover, glycerol, corn starch, sorghum, molasses, waste glycerol, and other renewable materials. These processes further comprise hydrotreating, hydrocracking, isomerization, distillation, and other petrochemical processes for use with oil-bearing microbes and products derived therefrom to manufacture fuels. Particular embodiments include the manufacture of ASTM D975 and ASTM D1655 compliant fuels. Genetically engineered microbes provided herein can be used in the manufacture of renewable diesel and renewable jet fuel.

公开(公告)号：US10119152B2

公开(公告)日：2018-11-06

申请号：US15090293

申请日：2016-04-04

申请人： Vogelbusch GmbH

发明人： Michael Sauer ,  Hans Marx ,  Stefan Pfluegl ,  Diethard Mattanovich

IPC分类号： C12P7/18 ,  C12N1/32 ,  C12N9/04 ,  C12P7/56 ,  C12R1/225

摘要： The invention refers to a method of biotransforming a carbohydrate of a raw material into a chemical, by cultivating Lactobacillus diolivorans in the presence of the raw material to produce a chemical substance, and isolating the chemical substance in the purified form, and the use of L. diolivorans in one of a series of biotransformation methods, wherein carbohydrates from at least two different carbohydrate sources of low purity are transformed into chemicals.

公开(公告)号：US20180119090A1

公开(公告)日：2018-05-03

申请号：US15856667

申请日：2017-12-28

申请人： Research Foundation of the City University of New York

发明人： Peter Lipke ,  Uthama Edupuganti

IPC分类号： C12N1/38 ,  C12N1/16 ,  C12N1/32

CPC分类号： C12N1/38 ,  C12N1/16 ,  C12N1/18 ,  C12N1/32 ,  C12P19/04

摘要： The present invention relates to a process for enhancing the growth and increasing the biomass of yeast cultures. The addition of ethanol during log growth phase increases the yield of yeast biomass and products purified from the biomass.

公开(公告)号：US20180119089A1

公开(公告)日：2018-05-03

申请号：US15590925

申请日：2017-05-09

申请人： Massachusetts Institute of Technology

发明人： Janelle Renee Thompson ,  Kyle C. Peet ,  Adam J.E. Freedman ,  Kristala Lanett Jones Prather ,  Jason Thomas Boock ,  Michael T. Timko

IPC分类号： C12N1/32 ,  C12R1/07 ,  C12Q1/68 ,  C12P7/64 ,  C12N15/00

CPC分类号： C12N1/32 ,  C12M1/06 ,  C12M21/00 ,  C12M41/40 ,  C12N1/20 ,  C12N9/88 ,  C12N15/00 ,  C12P7/04 ,  C12P7/16 ,  C12P7/22 ,  C12P7/6427 ,  C12P7/6472 ,  C12Q1/68 ,  C12R1/07 ,  Y02E50/10 ,  Y02P20/544

摘要： Provided herein are methods of producing a bioproduct by culturing a cell in a multiphase reactor that comprises supercritical CO2, near critical CO2, or liquid CO2.