公开(公告)号：US5229113A

公开(公告)日：1993-07-20

申请号：US393081

申请日：1989-08-09

申请人： Renee Kosslak ,  Roger Bookland ,  Edward R. Appelbaum

发明人： Renee Kosslak ,  Roger Bookland ,  Edward R. Appelbaum

IPC分类号： A01C1/00 ,  A01N63/00 ,  C05F11/08 ,  C07K14/195 ,  C12N15/74

CPC分类号： C12N15/74 ,  A01C1/00 ,  A01N63/00 ,  C05F11/08 ,  C07K14/195 ,  Y10S435/878 ,  Y10S47/09 ,  Y10S47/10

摘要： The chemical compounds that induce expression of nodulation genes of Bradyrhizobium japonicum strains have been identified. The isoflavones daidzein and genistein were identified as soybean exudate components that induce nod-genes. Other isoflavones, including 7-hydroxyisoflavone, 5,7-dihydroxyisoflavone, biochanin A, formononetin and prunetin, were also found to act as inducers. In addition, several flavonoid compounds including 4',7-dihydroxyflavone, apigenin, kaempferol and coumestrol, were also found to act as inducers. Nodulation inducing compositions and a method for selectively activating the expression of a gene under the control of a soybean exudate inducible promoter in B. japonicum in response to these inducer molecules are described. In addition, soybean inoculating compositions containing the nod-gene inducers and inoculating methods employing these compositions are described.

摘要翻译： 已经鉴定了引起日本。Brad of of of of结节基因表达的化合物。 异黄酮黄豆苷原和染料木素被鉴定为诱导结节基因的大豆渗出物成分。 还发现其他异黄酮，包括7-羟基异黄酮，5,7-二羟基异黄酮，鹰嘴豆素A，角叉菜素和杏仁素，作为诱导剂。 此外，还发现了包括4'，7-二羟基黄酮，芹菜素，山奈酚和香豆素的几种类黄酮化合物作为诱导剂。 描述了结瘤诱导组合物和用于选择性地激活在这些诱导剂分子响应于日本粳稻的大豆渗出物诱导型启动子控制下的基因表达的方法。 此外，还描述了含有点状基因诱导剂的大豆接种组合物和使用这些组合物的接种方法。

公开(公告)号：US5023180A

公开(公告)日：1991-06-11

申请号：US61848

申请日：1987-06-11

申请人： Edward R. Appelbaum ,  Hauke Hennecke ,  Joseph W. Lamb ,  Michael Gottfert

发明人： Edward R. Appelbaum ,  Hauke Hennecke ,  Joseph W. Lamb ,  Michael Gottfert

IPC分类号： C07K14/195 ,  C12N15/74

CPC分类号： C07K14/195 ,  C12N15/743 ,  C07K2319/00 ,  C07K2319/61 ,  Y10S435/878

摘要： Nodulation regulatory genes (nodD genes) of Bradyrhizobium japonicum strains have been isolated and sequenced. Recombinant DNA molecules and vectors containing these regulatory genes are described. These genes, molecules and vectors are useful in the genetic engineering of Rhizobium and Bradyrhizobium strains. A method for selective expression of structural genes in response to the application of chemical factors which induce B. japonicum nod genes which employs these genes is described.

摘要翻译： 已经分离并测序了Bradyrhizobium japonicum菌株的结瘤调节基因（nodD基因）。 描述了重组DNA分子和含有这些调节基因的载体。 这些基因，分子和载体可用于根瘤菌和Bradyrhizobium菌株的遗传工程。 描述了应用这些诱导使用这些基因的日本粳稻节点基因的化学因子的应用来选择性表达结构基因的方法。

公开(公告)号：US5001061A

公开(公告)日：1991-03-19

申请号：US109868

申请日：1987-10-16

申请人： Barry G. Rolfe ,  John Shine ,  Kieran F. Scott ,  Hauke Hennecke ,  Kieran F. Scott

发明人： Barry G. Rolfe ,  John Shine ,  Kieran F. Scott ,  Hauke Hennecke ,  Kieran F. Scott

IPC分类号： C07K14/325 ,  C07K14/825 ,  C12N9/02 ,  C12N15/74 ,  C12N15/90

CPC分类号： C12N9/0095 ,  C07K14/325 ,  C07K14/825 ,  C12N15/743 ,  C12N15/90 ,  C12Y118/06001

摘要： The nifD promoter regions of Bradyrhizobium japonicum and Bradyhizobium sp. (Parasponia) have been sequenced and found to be significantly homologous. Recombinant DNA molecules comprising foreign genes under the control of such promoters are provided. Rhizobial species containing such recombinant constructions, either in plasmids or integrated into the genome, are provided. These are especially useful for expressing desired foreign genes within root nodules.

摘要翻译： 日本Bradyrhizobium japonicum和Bradyhizobium sp。的nifD启动子区 （Parasponia）已被测序并发现是显着同源的。 提供了包含这种启动子控制下的外源基因的重组DNA分子。 提供了含有质粒或整合到基因组中的重组构建物的根瘤菌物种。 这些对于在根结节内表达所需的外来基因特别有用。

公开(公告)号：US4885357A

公开(公告)日：1989-12-05

申请号：US184348

申请日：1988-04-21

申请人： Brian Larkins ,  Richard E. Cuellar ,  Wallace John C.

发明人： Brian Larkins ,  Richard E. Cuellar ,  Wallace John C.

IPC分类号： C07K14/425 ,  C12N15/82 ,  C12P21/02

CPC分类号： C12N15/8251 ,  C07K14/425 ,  C12N15/8253 ,  C12N15/8254 ,  C12P21/02

摘要： Modified 19kd and 22kd zeins containing lysine are provided. Lysine additions are made by modifying structural genes which encode 19kd and 22kd pre-zeins. Modified zeins produced from the modified structural gene templates are found to retain important properties of unmodified zeins, having similar molecular weights, having the ability to form protein bodies within the rough endoplasmic reticulum of a host cell and being soluble in alcohol.

摘要翻译： 提供了含有赖氨酸的19kd和22kd的玉米蛋白质。 通过修饰编码19kd和22kd前原蛋白的结构基因来制备赖氨酸添加物。 发现由修饰的结构基因模板产生的修饰的玉米醇溶蛋白保留了具有相似分子量的未修饰玉米蛋白的重要性质，具有在宿主细胞的粗糙内质网内形成蛋白质体并且可溶于醇的能力。

公开(公告)号：US4885248A

公开(公告)日：1989-12-05

申请号：US12739

申请日：1987-03-09

申请人： Paul G. Ahlquist

发明人： Paul G. Ahlquist

IPC分类号： C12N15/73

CPC分类号： C12N15/73

摘要： A recombinant DNA vector is provided as a universal transcription vector having a replication origin and selectable marker, a promoter and a transcription initiation site comprising a first transcribed nucleotide, wherein a restriction site is provided immediately adjacent to and upstream from the transcription initiation site so as to separate transcribed from untranscribed nucleotides. A second restriction site may also be positioned downstream from the said restriction site. Precise control of initiation and termination of transcription is attained by this invention. Such control is important in assuring the effectiveness of transcribed RNA viral vectors.A high fidelity in vitro RNA transcription method is also provided utilizing vectors constructed from the universal transcription vector, or other vectors producing transcripts having no more than one extra 5' base. This method is capable of producing functional RNA transcripts, preferably comprising infectious viral sequences.

摘要翻译： 提供重组DNA载体作为具有复制起点和选择性标记的通用转录载体，启动子和包含第一转录核苷酸的转录起始位点，其中在转录起始位点的紧邻和上游提供限制性位点，以便 分离转录自未转录的核苷酸。 第二限制性位点也可以位于所述限制性位点的下游。 通过本发明实现了转录起始和终止的精确控制。 这种控制对于确保转录的RNA病毒载体的有效性是重要的。 使用由通用转录载体构建的载体或产生具有不超过一个额外的5'碱基的转录物的其他载体也提供高保真体外RNA转录方法。 该方法能够产生功能性RNA转录物，优选包含感染性病毒序列。

公开(公告)号：US4857465A

公开(公告)日：1989-08-15

申请号：US936812

申请日：1986-12-02

申请人： Usha B. Barwale ,  Jack M. Widholm

发明人： Usha B. Barwale ,  Jack M. Widholm

IPC分类号： A01H1/04 ,  A01H1/02 ,  A01H1/06 ,  A01H4/00 ,  A01H5/00 ,  A01H5/10 ,  C12N5/00

CPC分类号： A01H5/10 ,  A01H1/02 ,  A01H4/00 ,  C12N5/0025

摘要： A method is provided for the production of organogenic tissue culture and regeneration of whole plants of Glycine species, preferably Glycine max, involving the use of an organogenic culture medium comprising high cytokinin, preferably at least about 10 .mu.M BAP, and also preferably comprising at least about 6 times normal concentration of MS micronutrients. The organogenic culture medium is useful for the production of plants embodying desirable characteristics induced by somaclonal variation.

摘要翻译： 提供了一种方法，用于生产组织培养和再生整个植物的甘氨酸，优选大豆，涉及使用包含高细胞分裂素，优选至少约10μMBAP的组织培养基，并且还优选包含 至少约6倍正常浓度的MS微量营养素。 有机生长培养基可用于生产体细胞克隆变异诱导的所需特征的植物。

公开(公告)号：US5215912A

公开(公告)日：1993-06-01

申请号：US393834

申请日：1989-08-14

申请人： Leslie M. Hoffman

发明人： Leslie M. Hoffman

IPC分类号： C07K14/425 ,  C12N15/82

CPC分类号： C12N15/8251 ,  C07K14/425

摘要： The present invention discloses dicot cells containing monocot seed storage protein. Construction of genes encoding monocot seed storage proteins which are expressible in dicot cells and transformation of such genes into plant cells is also taught. Furthermore, methods and DNA molecules useful for producing plant cells containing monocot seed storage proteins are also disclosed. The invention is exemplified by combination of a 15 kD zein structural gene from Zea mays gene with a promoter and a polyadenylation site derived from a Phaseolus vulgaris phaseolin gene.

摘要翻译： 本发明公开了含有单子叶种子贮藏蛋白的双子叶植物细胞。 还教导了编码在双子叶植物细胞中可表达并且将这些基因转化到植物细胞中的单子叶种子储存蛋白的基因的构建。 此外，还公开了可用于产生含有单子叶种子储存蛋白的植物细胞的方法和DNA分子。 本发明通过将来自玉蜀黍基因的15kD玉米醇溶蛋白结构基因与启动子的组合和来自菜豆提取物的Phaseolus Phaseolin基因的多聚腺苷酸化位点进行组合。

公开(公告)号：US5053331A

公开(公告)日：1991-10-01

申请号：US198781

申请日：1988-05-25

申请人： Adrienne Clarke ,  Shaio Lim Mau ,  Marilyn Anderson ,  Edwina Cornish ,  Hugh D. Niall ,  Geoffrey W. Tregear ,  Robert J. Crawford ,  Robert Bernatzky

发明人： Adrienne Clarke ,  Shaio Lim Mau ,  Marilyn Anderson ,  Edwina Cornish ,  Hugh D. Niall ,  Geoffrey W. Tregear ,  Robert J. Crawford ,  Robert Bernatzky

IPC分类号： A01H1/00 ,  C07K14/415 ,  C12N15/29 ,  C12N15/62 ,  C12N15/82 ,  C12Q1/68

CPC分类号： C12N15/625 ,  C07K14/415 ,  C12N15/8287 ,  C12Q1/6895 ,  C07K2319/02

摘要： DNA sequence of S-genes which encode S-proteins and control the self-incompatibility reaction in gametophytic self-incompatible plants have been identified. The DNA sequence encoding several S-proteins of N. alata and their attendant signal sequences are specifically provided. Regulatory sequences which direct expression of the S-genes in reproduction tissue of self-incompatible plants have also been identified. A method for the identification and isolation of cDNA and genomic DNA coding sequences of the S-genes is described.

摘要翻译： 已经鉴定了编码S-蛋白并控制配子体自身不相容植物自交不亲和性反应的S基因的DNA序列。 具体提供编码N. alata的几种S蛋白的DNA序列及其伴随的信号序列。 也鉴定了在自交不亲和植物的繁殖组织中指导S基因表达的调控序列。 描述了用于鉴定和分离S基因的cDNA和基因组DNA编码序列的方法。

公开(公告)号：US4818696A

公开(公告)日：1989-04-04

申请号：US616950

申请日：1984-06-01

申请人： Edward R. Appelbaum ,  Thomas J. McLoughlin ,  Michael P. O'Connell

发明人： Edward R. Appelbaum ,  Thomas J. McLoughlin ,  Michael P. O'Connell

IPC分类号： C07K14/195 ,  C12N15/74 ,  C12N15/00 ,  C07H21/00 ,  C12N1/20 ,  C12R1/41

CPC分类号： C12R1/41 ,  C07K14/195 ,  C12N15/743 ,  Y10S435/878

摘要： A symbiosis plasmid from a fast-growing Rhizobium japonicum donor strain can be transferred to Rhizobium recipient strains, the recipient strains being previously incapable of forming an effective symbiotic relationship with plants of certain Glycine (e.g. soybean) varieties. The recipient strains harboring the symbiosis plasmid will form effective symbioses with soybean plants of said certain varieties, the transferred symbiosis plasmid conferring the host range or specificity of the donor strain on the recipient strains. Methods, strains, and plasmids useful for practice of this invention are also provided.

摘要翻译： 来自快速生长的日本根瘤菌供体菌株的共生质粒可以转移到根瘤菌受体菌株中，受体菌株以前不能与某些甘氨酸（例如大豆）品种的植物形成有效的共生关系。 携带共生质粒的受体菌株将与所述某些品种的大豆植物形成有效的共生，转移的共生质粒赋予受体菌株的供体菌株的宿主范围或特异性。 还提供了可用于实践本发明的方法，菌株和质粒。

公开(公告)号：US4771002A

公开(公告)日：1988-09-13

申请号：US584244

申请日：1984-02-24

申请人： Stanton B. Gelvin

发明人： Stanton B. Gelvin

IPC分类号： A01H5/00 ,  A01H1/00 ,  C12N1/20 ,  C12N1/21 ,  C12N5/00 ,  C12N5/10 ,  C12N15/00 ,  C12N15/09 ,  C12N15/70 ,  C12N15/82 ,  C12R1/01 ,  C12R1/19 ,  C12R1/91

CPC分类号： C12N15/70 ,  C12N15/8216

摘要： A promoter region that drives expression of a 1450 base T.sub.R transcript in octopine-type crown gall tumors can also promote expression of a foreign structural gene in bacteria. Use of this dul-purpose promoter region to drive expression of a single copy of a foreign structural gene in both plants and bacteria is taught. The construction of a selectable marker functional in eukaryotes and prokaryotes is exemplified, as are vectors useful in efforts to transform plants.

摘要翻译： 在章鱼型冠状胆囊肿瘤中驱动1450碱基TR转录物表达的启动子区域也可促进外源结构基因在细菌中的表达。 教导了这种多用途启动子区域驱动外来结构基因在植物和细菌中的单拷贝表达的用途。 例证了在真核生物和原核生物中功能的选择性标记的构建，以及可用于转化植物的载体。