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    Enzyme Hyaluronan-Lyase, Method of Production Thereof, Use Thereof and Method of Preparation of Low-Molecular Hyaluronan
    1.
    发明申请
    Enzyme Hyaluronan-Lyase, Method of Production Thereof, Use Thereof and Method of Preparation of Low-Molecular Hyaluronan 审中-公开
    酵素透明质酸裂解酶，其生产方法及其用途及低分子透明质酸的制备方法

    公开(公告)号：US20150344926A1

    公开(公告)日：2015-12-03

    申请号：US14430731

    申请日：2013-09-26

    申请人： CONTIPRO BIOTECH S.R.O.

    发明人： Lenka Knorova ,  Dzianis Smirnou ,  Veronika Leierova ,  Martin Krcmar ,  Lukas Franke ,  Vladimir Velebny

    IPC分类号： C12P19/26 ,  C12N9/88

    CPC分类号： C12P19/26 ,  C12N9/88 ,  C12P19/04 ,  C12P19/12 ,  C12Y402/02001

    摘要： The invention relates to an enzyme which is able to degrade hyaluronic acid and which is produced by fungi of the genus Fistulina (especially Fistulina hepatica). The degradation proceeds by lyase mechanism in which double bonds between C4 and C5 of glucuronic acid are formed. The invention also includes the process of preparation and purification of the enzyme and a possible practical use thereof for the preparation of low-molecular hyaluronan or of cosmetic or pharmaceutical devices. Further, the invention relates to the method of preparation of low-molecular hyaluronan.

    摘要翻译： 本发明涉及一种能够降解透明质酸并由瘘管真菌（特别是肝瘘）产生的酶。 通过裂解酶机理进行降解，其中形成葡萄糖醛酸的C4和C5之间的双键。 本发明还包括酶的制备和纯化方法及其可用于制备低分子透明质酸或化妆品或药物装置的实际应用。 此外，本发明涉及低分子透明质酸的制备方法。

    Hyaluronic Acid Derivative, Method of Preparation Thereof, Method of Modification Thereof and Use Thereof
    3.
    发明申请
    Hyaluronic Acid Derivative, Method of Preparation Thereof, Method of Modification Thereof and Use Thereof 有权
    透明质酸衍生物，其制备方法，其改性方法及用途

    公开(公告)号：US20150175717A1

    公开(公告)日：2015-06-25

    申请号：US14420012

    申请日：2013-08-08

    申请人： Contipro Biotech s.r.o.

    发明人： Radovan Buffa ,  Petra Sedova ,  Vladimir Velebny ,  Lucie Wolfova ,  Ivana Basarabova ,  Robert Pospisil ,  Martina Hasova ,  Kristina Nesporova

    IPC分类号： C08B37/08 ,  A61K47/48 ,  C07K17/10

    CPC分类号： C08B37/0072 ,  A61K47/61 ,  C07K17/10 ,  C08J3/075 ,  C08J2305/00

    摘要： The invention relates to the preparation and use of α,β-unsaturated aldehyde of hyaluronan having a double bond in the positions 4 and 5 and an aldehydic group in the position 6 of the glucosamine part of the polysaccharide. The method of preparation is based on dehydration of hyaluronan having an aldehydic group in the position 6 of the glucosamine part of the polysaccharide. Two methods have been described, which are dehydration in a solution or heating in solid state in absence of solvents, bases or other additives. This derivative allows stabilization of conjugates of hyaluronan with amino compounds by means of a multiple bond from the aldehyde side, and therefore, it is possible to effectively immobilize practically any compound containing an amino group to such modified hyaluronan in physiological conditions. In case of using a diamine or compounds or polymers containing three or more amino groups, it is possible to prepare crosslinked hyaluronan derivatives. The described solution brings along a significant advantage not only in the field of carriers of biologically active substances, but also in tissue engineering where crosslinking with biologically acceptable amino compounds in physiological conditions is very much demanded.

    摘要翻译： 本发明涉及在多糖的葡糖胺部分中位置4和5具有双键的透明质酸α和β-不饱和醛的制备和用途以及位置6的醛基。 制备方法是基于在多糖的葡糖胺部分的位置6中具有醛基的透明质酸的脱水。 已经描述了两种方法，其在溶液中脱水或在没有溶剂，碱或其它添加剂的情况下以固态加热。 该衍生物允许透明质酸与氨基化合物的缀合物通过来自醛侧的多重键进行稳定化，因此，有可能在生理条件下实际上将含有氨基的任何化合物有效地固定在这种修饰的透明质酸上。 在使用二胺或含有三个以上氨基的化合物或聚合物的情况下，可以制备交联的透明质酸衍生物。 所描述的解决方案不仅在生物活性物质的载体领域具有显着的优点，而且在组织工程中，其中非常需要在生理条件下与生物可接受的氨基化合物进行交联。

    SPINNING NOZZLE FOR PRODUCING NANOFIBROUS AND MICROFIBROUS MATERIALS COMPOSED OF FIBRES HAVING A COAXIAL STRUCTURE
    4.
    发明申请
    SPINNING NOZZLE FOR PRODUCING NANOFIBROUS AND MICROFIBROUS MATERIALS COMPOSED OF FIBRES HAVING A COAXIAL STRUCTURE 审中-公开
    用于生产纳米纤维的旋转喷嘴和具有同轴结构的纤维组成的微晶材料

    公开(公告)号：US20150152572A1

    公开(公告)日：2015-06-04

    申请号：US14416783

    申请日：2013-07-23

    申请人： Contipro Biotech S.R.O.

    发明人： Marek Pokorny ,  Jiri Rebicek ,  Lada Sukova ,  Jindrich Novak ,  Jana Novakova ,  Vladimir Velebny

    IPC分类号： D01D5/00 ,  D01D5/34

    CPC分类号： D01D5/0069 ,  D01D4/02 ,  D01D5/30 ,  D01D5/34

    摘要： Spinning nozzle for production of nanofibrous and microfibrous materials comprises: a first plate (1) provided with at least one continuous groove (2) for directing a first material to an outlet mouth portion (3) of the continuous groove (2) in a face (4) of the first plate (1); a second plate (5) provided with at least one continuous groove (6) for directing a second material to an outlet mouth portion (7) of the continuous groove (6) of the second plate (5), the outlet mouth portion (7) being arranged adjacent to the outlet mouth portion (3) of the first plate (1); and a separating plate (8) arranged between the first plate (1) and the second plate (5) for separating the continuous grooves (2) of the first plate (1) from the continuous grooves (6) of the second plate (5), while the face (9) of the separating plate (8) forms with the face (4) of the first plate (1) and/or the face (10) of the second plate (5) a continuous surface in the region of the outlet mouth portions (3; 7) of the continuous grooves (2; 6).

    摘要翻译： 用于制造纳米纤维和微纤维材料的纺丝喷嘴包括：第一板（1），设置有至少一个连续的凹槽（2），用于将第一材料引导到连续凹槽（2）的出口口部分（3） （1）的第一（4）; 设置有至少一个连续槽（6）的第二板（5），用于将第二材料引导到第二板（5）的连续槽（6）的出口口部分（7），出口口部分 ）邻近所述第一板（1）的出口口部分（3）布置; 以及布置在所述第一板（1）和所述第二板（5）之间的用于将所述第一板（1）的连续槽（2）与所述第二板（5）的连续槽（6）分离的分离板（8） ），而分离板（8）的面（9）与第一板（1）的面（4）和/或第二板（5）的面（10）形成区域中的连续表面 的连续槽（2; 6）的出口口部分（3; 7）。

    Crosslinked Hyaluronan Derivative, Method of Preparation Thereof, Hydrogel and Microfibers Based Thereon
    7.
    发明申请
    Crosslinked Hyaluronan Derivative, Method of Preparation Thereof, Hydrogel and Microfibers Based Thereon 审中-公开
    交联透明质酸衍生物，其制备方法，水凝胶和基于其的微纤维

    公开(公告)号：US20150104643A1

    公开(公告)日：2015-04-16

    申请号：US14395575

    申请日：2013-04-25

    申请人： Contipro Biotech s.r.o.

    发明人： Gloria Huerta-Angeles ,  Zuzana Jouklova ,  Eva Prikopova ,  Vladimir Velebny

    IPC分类号： C08B37/08

    CPC分类号： C08B37/0072 ,  D01D5/06 ,  D01F9/00 ,  Y10T428/298

    摘要： The invention relates to a crosslinked hyaluronan derivative in the form of a hydrogel or microfibers, and to a method of preparation thereof, consisting in a C—C coupling reaction. The C—C coupling reaction is performed via reaction of a hyaluronan derivative carrying a terminal aryl-halide and/or aryl-borate group, and a hyaluronan derivative carrying an alkenyl or alkynyl group, in water, phosphates buffer or a mixture of an organic acid and an alcohol, and in the presence of a palladium active catalyst. The palladium active catalyst may be e.g. a complex of palladium (II) acetate and an inorganic or organic base, or a complex of palladium (II) and 2-amino-4,6-dihydroxypyrimidine.

    摘要翻译： 本发明涉及水凝胶或微纤维形式的交联透明质酸衍生物及其制备方法，由C-C偶联反应组成。 C-C偶联反应通过带有末端芳基卤化物和/或芳基硼酸盐基团的透明质酸衍生物和带有烯基或炔基的透明质酸衍生物在水，磷酸盐缓冲液或有机 酸和醇，并在钯活性催化剂存在下进行。 钯活性催化剂可以是例如。 乙酸钯（II）和无机或有机碱的络合物，或钯（II）和2-氨基-4,6-二羟基嘧啶的络合物。

    Combined spinning nozzle for the manufacture of nanofibrous and microfibrous materials
    8.
    发明授权
    Combined spinning nozzle for the manufacture of nanofibrous and microfibrous materials 有权
    用于制造纳米纤维和微纤维材料的组合纺丝喷嘴

    公开(公告)号：US08727756B2

    公开(公告)日：2014-05-20

    申请号：US13737278

    申请日：2013-01-09

    申请人： Contipro Biotech, s.r.o.

    发明人： Marek Pokorný ,  Lada Suková ,  Ji{hacek over (r)}i {hacek over (R)}ebí{hacek over (c)}ek ,  Vladimír Velebný

    IPC分类号： D01D5/00

    CPC分类号： D01D5/0061 ,  D01D5/0069 ,  D01D5/14

    摘要： The combined spinning nozzle for the production of nanofibrous or microfibrous materials comprises a thin-walled electrode (1) and a first non-conductive body (2) adjoining the first wall of said thin-walled electrode, said first body having its wall, which faces the thin-walled electrode (1), provided with an array of grooves (5) formed therein, said grooves leading to the distal end (6) of the combined spinning nozzle and having their proximal ends connected to a supply of spinning mixture. The thin-walled electrode (1) and the first non-conductive body (2) may assume either plate-like or cylindrical shapes. The combined spinning nozzle may further comprise a second non-conductive body (4) adjoining a second wall of the thin-walled electrode (1) and directing the air from the proximal end towards the distal end (6) of the nozzle.

    摘要翻译： 用于生产纳米纤维或微纤维材料的组合纺丝喷嘴包括薄壁电极（1）和与所述薄壁电极的第一壁相邻的第一非导电体（2），所述第一体具有其壁， 面对薄壁电极（1），其设置有形成在其中的凹槽阵列（5），所述凹槽通向组合的纺丝喷嘴的远端（6），并且其近端连接到纺丝混合物的供应。 薄壁电极（1）和第一非导电体（2）可以采用板状或圆柱形。 组合的纺丝喷嘴还可包括毗邻薄壁电极（1）的第二壁并将空气从近端朝向喷嘴的远端（6）引导的第二非导电体（4）。