公开(公告)号：US08597594B2

公开(公告)日：2013-12-03

申请号：US13551704

申请日：2012-07-18

申请人： Jonathan Posner ,  Kamil Salloum ,  Michal Lebl ,  Mark Reed ,  Dale Buermann ,  Matthew Hage ,  Bryan Crane ,  David Heiner ,  Robert Kain ,  Michael Schroeder

发明人： Jonathan Posner ,  Kamil Salloum ,  Michal Lebl ,  Mark Reed ,  Dale Buermann ,  Matthew Hage ,  Bryan Crane ,  David Heiner ,  Robert Kain ,  Michael Schroeder

IPC分类号： B01L3/00

CPC分类号： F04B37/10 ,  F04B19/00 ,  Y10T436/2575

摘要： An apparatus for fragmenting nucleic acid. The apparatus includes a sample reservoir that comprises a fluid having nucleic acids. The apparatus can also include a shear wall that is positioned within the sample reservoir. The shear wall includes a porous core medium that has pores that are sized to permit nucleic acids to flow therethrough. The apparatus also includes first and second chambers that are separated by the shear wall. The first and second chambers are in fluid communication with each other through the porous core medium of the shear wall. Also, the apparatus may include first and second electrodes that are located within the first and second chambers, respectively. The first and second electrodes are configured to generate an electric field that induces a flow of the sample fluid. The nucleic acids move through the shear wall thereby fragmenting the nucleic acids.

摘要翻译： 核酸分裂装置。 该装置包括包含具有核酸的流体的样品储存器。 该装置还可以包括位于样品容器内的剪切壁。 剪切壁包括多孔芯介质，其具有尺寸允许核酸流过其中的孔。 该装置还包括被剪力墙隔开的第一和第二室。 第一和第二腔室通过剪切壁的多孔纤维介质彼此流体连通。 此外，该装置可以包括分别位于第一和第二室内的第一和第二电极。 第一和第二电极被配置成产生诱发样品流体流动的电场。 核酸通过剪切壁移动，从而使核酸分裂。

公开(公告)号：US08481903B2

公开(公告)日：2013-07-09

申请号：US13041194

申请日：2011-03-04

申请人： Alexander Triener ,  Erik Allegoren ,  Wenyi Feng ,  Dale Buermann ,  Erik Olson ,  James Osmus

发明人： Alexander Triener ,  Erik Allegoren ,  Wenyi Feng ,  Dale Buermann ,  Erik Olson ,  James Osmus

IPC分类号： G02B7/04

CPC分类号： C12Q1/6874 ,  C12Q1/6837 ,  G01N21/6428 ,  G01N21/645 ,  G01N21/6456

摘要： An optical system configured to detect optical signals during imaging sessions. The optical system includes an objective lens that has a collecting end that is positioned proximate to a sample and configured to receive optical signals therefrom. The optical system also includes a removable path compensator that is configured to be located at an imaging position between the collecting end of the objective lens and the sample. The path compensator adjusts an optical path of the light emissions when in the imaging position. Also, the optical system includes a transfer device that is configured to move the path compensator. The transfer device locates the path compensator at the imaging position for a first imaging session and removes the path compensator from the imaging position for a second imaging session.

摘要翻译： 一种配置成在成像会话期间检测光信号的光学系统。 光学系统包括物镜，该物镜具有位于样品附近并被配置为从其接收光信号的收集端。 光学系统还包括被配置为位于物镜的收集端和样品之间的成像位置处的可移除路径补偿器。 路径补偿器在成像位置调整光发射的光路。 此外，光学系统包括被配置为移动路径补偿器的传送装置。 传送装置将路径补偿器定位在用于第一成像会话的成像位置处，并从第二成像会话的成像位置移除路径补偿器。

公开(公告)号：US20100111768A1

公开(公告)日：2010-05-06

申请号：US12295337

申请日：2007-03-30

申请人： Saibal Banerjee ,  Colin Barnes ,  Kevin Benson ,  John Bridgham ,  Jason Bryant ,  Dale Buermann ,  Sergey Etchin ,  Jonny Ho ,  Xavier Lee ,  Peter Lundberg ,  Klaus Maisinger ,  Bojan Obradovic ,  Mark Pratt ,  Isabelle Rasolonjatovo ,  Mark Reed ,  Chiara Rodighiero ,  Subra Sankar ,  Gary Schroth ,  Ning Sizto ,  Harold Swerdlow ,  Eric Vermaas

发明人： Saibal Banerjee ,  Colin Barnes ,  Kevin Benson ,  John Bridgham ,  Jason Bryant ,  Dale Buermann ,  Sergey Etchin ,  Jonny Ho ,  Xavier Lee ,  Peter Lundberg ,  Klaus Maisinger ,  Bojan Obradovic ,  Mark Pratt ,  Isabelle Rasolonjatovo ,  Mark Reed ,  Chiara Rodighiero ,  Subra Sankar ,  Gary Schroth ,  Ning Sizto ,  Harold Swerdlow ,  Eric Vermaas

IPC分类号： C12Q1/68 ,  C12M3/00

CPC分类号： C12Q1/6869 ,  C12Q2535/122 ,  C12Q2565/60 ,  G01N21/6456

摘要： The present invention comprises systems and devices for sequencing of nucleic acid, such as short DNA sequences from clonally amplified single-molecule arrays.

摘要翻译： 本发明包括用于核酸测序的系统和装置，例如来自克隆扩增的单分子阵列的短DNA序列。

公开(公告)号：US20050280804A1

公开(公告)日：2005-12-22

申请号：US10871496

申请日：2004-06-18

申请人： Dale Buermann ,  Michael Mandella

发明人： Dale Buermann ,  Michael Mandella

IPC分类号： G01B11/26 ,  G01C1/00 ,  G01S5/16 ,  G01S17/48 ,  H04N7/18 ,  H04N9/47

CPC分类号： G01S5/163 ,  G01B11/26 ,  G01S17/48

摘要： An elongate object optically determines at least one of its orientation parameters relative to a plane surface. A probe radiation beam is directed from the object at various angles σ to various locations on the plane, where the angle σ is a periodic function of time. Two angularly-selective radiation detectors oriented at fixed angles τ1 and τ2 sense scattered portions of the beam from two locations a two corresponding times. The orientation parameter is computed from a time difference Δt=t2−t1 between the two times.

摘要翻译： 细长物体相对于平面表面光学地确定其取向参数中的至少一个。 探针辐射束以不同的角度从物体引导到平面上的各种位置，其中角度σ是时间的周期函数。 以固定角度τ1和τ2定向的两个角度选择性辐射探测器从两个位置感测来自两个对应时间的波束的散射部分。 方向参数是从两次之间的时间差Deltat = t 2 -t 1 1 <1>计算的。

公开(公告)号：US5991022A

公开(公告)日：1999-11-23

申请号：US79875

申请日：1998-05-15

申请人： Dale Buermann ,  Abdul Rahim Forouhi ,  Michael J. Mandella

发明人： Dale Buermann ,  Abdul Rahim Forouhi ,  Michael J. Mandella

IPC分类号： G01B11/06 ,  G01J3/02 ,  G01J3/12 ,  G01J3/42 ,  G01J3/45 ,  G01J4/00 ,  G01N21/27 ,  G01N21/45

CPC分类号： G01J3/12 ,  G01B11/0625 ,  G01B11/0641 ,  G01J3/02 ,  G01J3/0208 ,  G01J3/42 ,  G01J4/00

摘要： An apparatus uses reflectance spectrophotometry to characterize a sample having any number of thin films. The apparatus uses two toroidal mirrors in an optical relay to direct light reflected by the sample to a spectrophotometer. A computer then analyzes the reflected spectrum to characterize the optical properties of the sample. The optical relay allows a range of angles of reflection from the sample, and has no chromatic aberration. The optical relay is also arranged so that the non-chromatic aberration is minimized. The sample is mounted on a movable stage so that different areas of the sample may be characterized. Furthermore, a deflector and a viewer are used to allow the operator of the apparatus to view the region of the sample under study.

摘要翻译： 一种装置使用反射分光光度法来表征具有任何数量薄膜的样品。 该装置在光学继电器中使用两个环形反射镜将由样品反射的光引导到分光光度计。 然后计算机分析反射光谱以表征样品的光学性质。 光学继电器允许来自样品的一定范围的反射角度，并且没有色差。 光学继电器也被布置成使得非色差最小化。 将样品安装在可移动的载物台上，以便可以对样品的不同区域进行表征。 此外，偏转器和观察器用于允许装置的操作者观察正在研究的样品的区域。

公开(公告)号：US20120292190A1

公开(公告)日：2012-11-22

申请号：US13551704

申请日：2012-07-18

申请人： Jonathan Posner ,  Kamil Salloum ,  Michal Lebl ,  Mark Reed ,  Dale Buermann ,  Matthew Hage ,  Bryan Crane ,  David Heiner ,  Robert Kain

发明人： Jonathan Posner ,  Kamil Salloum ,  Michal Lebl ,  Mark Reed ,  Dale Buermann ,  Matthew Hage ,  Bryan Crane ,  David Heiner ,  Robert Kain

IPC分类号： C25B7/00

CPC分类号： F04B37/10 ,  F04B19/00 ,  Y10T436/2575

摘要： An apparatus for fragmenting nucleic acid. The apparatus includes a sample reservoir that comprises a fluid having nucleic acids. The apparatus can also include a shear wall that is positioned within the sample reservoir. The shear wall includes a porous core medium that has pores that are sized to permit nucleic acids to flow therethrough. The apparatus also includes first and second chambers that are separated by the shear wall. The first and second chambers are in fluid communication with each other through the porous core medium of the shear wall. Also, the apparatus may include first and second electrodes that are located within the first and second chambers, respectively. The first and second electrodes are configured to generate an electric field that induces a flow of the sample fluid. The nucleic acids move through the shear wall thereby fragmenting the nucleic acids.

摘要翻译： 核酸分裂装置。 该装置包括包含具有核酸的流体的样品储存器。 该装置还可以包括位于样品容器内的剪切壁。 剪切壁包括多孔芯介质，其具有尺寸允许核酸流过其中的孔。 该装置还包括被剪力墙隔开的第一和第二室。 第一和第二腔室通过剪切壁的多孔纤维介质彼此流体连通。 此外，该装置可以包括分别位于第一和第二室内的第一和第二电极。 第一和第二电极被配置成产生诱发样品流体流动的电场。 核酸通过剪切壁移动，从而使核酸分裂。

公开(公告)号：US20120270305A1

公开(公告)日：2012-10-25

申请号：US13273666

申请日：2011-10-14

申请人： MARK T. REED ,  ERIC WILLIAMSON ,  BRYAN CRANE ,  PATRICK LEUNG ,  DALE BUERMANN ,  ALEXANDER P. KINDWALL ,  Frederick ERIE ,  MARK PRATT ,  JASON HARRIS ,  ANDREW JAMES CARSON ,  Stanley S. HONG ,  JASON BRYANT ,  MARK WANG ,  DREW VERKADE

发明人： MARK T. REED ,  ERIC WILLIAMSON ,  BRYAN CRANE ,  PATRICK LEUNG ,  DALE BUERMANN ,  ALEXANDER P. KINDWALL ,  Frederick ERIE ,  MARK PRATT ,  JASON HARRIS ,  ANDREW JAMES CARSON ,  Stanley S. HONG ,  JASON BRYANT ,  MARK WANG ,  DREW VERKADE

IPC分类号： C12M1/34 ,  G01N1/00 ,  G01N21/00 ,  B01L9/00

CPC分类号： B01L3/502715 ,  B01L7/52 ,  B01L9/527 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/04 ,  B01L2200/0689 ,  B01L2300/022 ,  B01L2300/041 ,  B01L2300/043 ,  B01L2300/0816 ,  B01L2300/0877 ,  G01N21/05 ,  G01N2021/058 ,  Y10T436/25

摘要： A fluidic device holder configured to orient a fluidic device. The device holder includes a support structure configured to receive a fluidic device. The support structure includes a base surface that faces in a direction along the Z-axis and is configured to have the fluidic device positioned thereon. The device holder also includes a plurality of reference surfaces facing in respective directions along an XY-plane. The device holder also includes an alignment assembly having an actuator and a movable locator arm that is operatively coupled to the actuator. The locator arm has an engagement end. The actuator moves the locator arm between retracted and biased positions to move the engagement end away from and toward the reference surfaces. The locator arm is configured to hold the fluidic device against the reference surfaces when the locator arm is in the biased position.

摘要翻译： 流体装置保持器，其构造成定向流体装置。 装置保持器包括构造成接收流体装置的支撑结构。 所述支撑结构包括在Z轴方向上面对并且被配置成具有定位在其上的流体装置的基面。 装置保持器还包括沿着XY平面在各个方向上面对的多个参考表面。 装置保持器还包括具有致动器和可操作地联接到致动器的可动定位臂的对准组件。 定位臂具有接合端。 致动器将定位臂移动到缩回位置和偏置位置之间，以使接合端远离参考表面移动。 定位臂构造成当定位臂处于偏置位置时将流体装置保持在参考表面上。

公开(公告)号：US20110220775A1

公开(公告)日：2011-09-15

申请号：US13041194

申请日：2011-03-04

申请人： ALEXANDER TRIENER ,  ERIK ALLEGOREN ,  WENYI FENG ,  DALE BUERMANN ,  ERIK OLSON ,  JAMES OSMUS

发明人： ALEXANDER TRIENER ,  ERIK ALLEGOREN ,  WENYI FENG ,  DALE BUERMANN ,  ERIK OLSON ,  JAMES OSMUS

IPC分类号： G01J3/50 ,  G01J1/02

CPC分类号： C12Q1/6874 ,  C12Q1/6837 ,  G01N21/6428 ,  G01N21/645 ,  G01N21/6456

摘要： An optical system configured to detect optical signals during imaging sessions. The optical system includes an objective lens that has a collecting end that is positioned proximate to a sample and configured to receive optical signals therefrom. The optical system also includes a removable path compensator that is configured to be located at an imaging position between the collecting end of the objective lens and the sample. The path compensator adjusts an optical path of the light emissions when in the imaging position. Also, the optical system includes a transfer device that is configured to move the path compensator. The transfer device locates the path compensator at the imaging position for a first imaging session and removes the path compensator from the imaging position for a second imaging session.

摘要翻译： 一种配置成在成像会话期间检测光信号的光学系统。 光学系统包括物镜，该物镜具有位于样品附近并被配置为从其接收光信号的收集端。 光学系统还包括被配置为位于物镜的收集端和样本之间的成像位置处的可移除路径补偿器。 路径补偿器在成像位置调整光发射的光路。 此外，光学系统包括被配置为移动路径补偿器的传送装置。 传送装置将路径补偿器定位在用于第一成像会话的成像位置处，并从第二成像会话的成像位置移除路径补偿器。

公开(公告)号：US20110072914A1

公开(公告)日：2011-03-31

申请号：US12031054

申请日：2008-02-14

申请人： Michal Lebl ,  Dale Buermann ,  Mark T. Reed ,  David L. Heiner ,  Alexander Triener

发明人： Michal Lebl ,  Dale Buermann ,  Mark T. Reed ,  David L. Heiner ,  Alexander Triener

IPC分类号： G01N1/14 ,  F04B37/10 ,  B01L3/02

CPC分类号： F04B19/006 ,  B01L3/502715 ,  B01L3/50273 ,  B01L7/00 ,  B01L2300/0816 ,  B01L2300/0877 ,  B01L2400/0418 ,  G01N35/00613 ,  G01N35/1095 ,  Y10T436/2575

摘要： A flows cell for use in a microfluidic detection system is provided. The flow cell includes a flows cell body having a channel that is configured to convey a solution through the flows cell body. The flow cell also includes a bottom surface and a top surface. The bottom surface is configured to be removably held by the detection system and the top surface is transparent and permits light to pass therethrough. The flow cell body also includes fluidic inlet and outlet ports that are in fluid communication with the channel. A pump cavity is also provided in the flow cell body. The pump cavity fluidly communicates with, and is interposed between, an end of the channel and one of the fluidic inlet and outlet ports. An electroosmotic (EO) pump is held in the pump cavity. The EO pump induces flow of the solution through the EO pump and channel between the fluidic inlet and outlet ports.

摘要翻译： 提供了一种用于微流体检测系统的流通池。 流动池包括具有通道的流动池主体，该通道被配置为将溶液输送通过流动池主体。 流动池还包括底面和顶面。 底表面被构造成由检测系统可移除地保持，并且顶表面是透明的并且允许光通过。 流动池体还包括与通道流体连通的流体入口和出口。 在流动池主体中还设有泵腔。 泵腔与通道的端部和流体入口和出口之一流体连通并且介于其间。 电渗（EO）泵保持在泵腔中。 EO泵通过EO泵和流体入口和出口之间的通道引起溶液的流动。

公开(公告)号：US20050195387A1

公开(公告)日：2005-09-08

申请号：US10796236

申请日：2004-03-08

申请人： Guanghua Zhang ,  Dale Buermann ,  Michael Mandella ,  Hector Gonzalez-Banos ,  Stewart Carl

发明人： Guanghua Zhang ,  Dale Buermann ,  Michael Mandella ,  Hector Gonzalez-Banos ,  Stewart Carl

IPC分类号： G01B11/26 ,  G01C1/00

CPC分类号： G01B11/002 ,  G01B11/272 ,  G01S17/46 ,  G06F3/0304 ,  G06F3/03545

摘要： An apparatus and method employing principles of stereo vision for determining one or more orientation parameters and especially the second and third Euler angles θ, ψ of an elongate object whose tip is contacting a surface at a contact point. The apparatus has a projector mounted on the elongate object for illuminating the surface with a probe radiation in a known pattern from a first point of view and a detector mounted on the elongate object for detecting a scattered portion of the probe radiation returning from the surface to the elongate object from a second point of view. The orientation parameters are determined from a difference between the projected and detected probe radiation such as the difference between the shape of the feature produced by the projected probe radiation and the shape of the feature detected by the detector. The pattern of probe radiation is chosen to provide information for determination of the one or more orientation parameters and can include asymmetric patterns such as lines, ellipses, rectangles, polygons or the symmetric cases including circles, squares and regular polygons. To produce the patterns the projector can use a scanning arrangement or a structured light optic such as a holographic, diffractive, refractive or reflective element and any combinations thereof. The apparatus is suitable for determining the orientation of a jotting implement such as a pen, pencil or stylus.

摘要翻译： 一种采用立体视觉原理的装置和方法，用于确定一个或多个取向参数，特别是尖端在接触点处接触表面的细长物体的第二和第三欧拉角θ，psi。 该装置具有安装在细长物体上的投影仪，用于从第一角度以已知图案的探测辐射照射表面，以及安装在细长物体上的检测器，用于检测从表面返回的探针辐射的散射部分 细长物体从第二个角度来看。 取向参数根据投射和检测的探针辐射之间的差异确定，例如由投射的探针辐射产生的特征的形状与由检测器检测到的特征的形状之间的差异。 选择探针辐射的图案以提供用于确定一个或多个取向参数的信息，并且可以包括不对称图案，例如线，椭圆，矩形，多边形或包括圆形，正方形和正多边形的对称情况。 为了产生图案，投影仪可以使用扫描装置或诸如全息衍射，折射或反射元件的结构化光学元件及其任何组合。 该装置适用于确定诸如笔，铅笔或触笔之类的记录工具的取向。