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公开(公告)号：US08753803B2
公开(公告)日：2014-06-17
申请号：US12878828
申请日：2010-09-09
申请人： Yoshihisa Kawamura , Eishi Shiobara , Shinichi Ito
发明人： Yoshihisa Kawamura , Eishi Shiobara , Shinichi Ito
IPC分类号： G03F7/20
CPC分类号： G03F7/0002
摘要： According to one embodiment, a pattern forming method is disclosed. The method can include selectively providing a curing agent to a pattern in a template, contacting the template provided the curing agent to a substrate, irradiating the curing agent with light where the template and the substrate are contacted each other to harden the curing agent, demolding the template from the substrate to form a curing agent pattern on the substrate, and etching the substrate on a basis of the curing agent pattern.
摘要翻译： 根据一个实施例，公开了一种图案形成方法。 该方法可以包括选择性地向模板中的图案提供固化剂，使提供固化剂的模板与基底接触，用光照射固化剂，其中模板和基底彼此接触以硬化固化剂，脱模 所述模板从所述基板在所述基板上形成固化剂图案，并且基于所述固化剂图案来蚀刻所述基板。
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公开(公告)号：US20120248065A1
公开(公告)日：2012-10-04
申请号：US13424837
申请日：2012-03-20
申请人： Yoshihisa KAWAMURA , Shinichi Ito
发明人： Yoshihisa KAWAMURA , Shinichi Ito
CPC分类号： H01L21/31144 , B05D1/42 , B05D3/067 , B05D5/02 , B82Y10/00 , B82Y40/00 , G03F7/0002 , H01L21/3105
摘要： According to one embodiment, a pattern forming methoo is disclosed. A film is formed on a substrate to be processed. A gaps is formed in a surface of the film. A photo-curable imprinting agent is supplied on the film surface in which the gaps are formed. The agent is contacted with a template including a concave pattern. The contacting is configured to fill the concave pattern with the agent. Light is applied to the agent while the agent is contacted with the template, wherein the agent is cured by the light. The template is separated from the substrate, wherein a pattern of the cured agent is formed on the substrate.
摘要翻译： 根据一个实施例，公开了形成图案的图案。 在被处理基板上形成膜。 在膜的表面形成间隙。 在其上形成间隙的膜表面上提供可光固化的压印剂。 该试剂与包括凹形图案的模板接触。 接触被配置成用该药剂填充凹形图案。 当试剂与模板接触时，将光施加到试剂上，其中试剂通过光固化。 将模板与基材分离，其中在基材上形成固化剂的图案。
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公开(公告)号：US20110159209A1
公开(公告)日：2011-06-30
申请号：US12977443
申请日：2010-12-23
申请人： Yoshihisa KAWAMURA , Shinichi Ito
发明人： Yoshihisa KAWAMURA , Shinichi Ito
CPC分类号： G03F7/0002 , B82Y10/00 , B82Y40/00
摘要： To forming a pattern on a substrate using a template simply and at low cost by forming a high-molecular copolymer having a first segment and a second segment on a substrate, contacting a template having a groove with the copolymer, filling the copolymer into the groove of the template, causing a phase separation of the copolymer to form a first phase having the first segment and a second phase having the second segment, releasing the template from the copolymer, and removing the first phase or the second phase of the copolymer.
摘要翻译： 为了通过在基板上形成具有第一段和第二段的高分子共聚物，简单且低成本地使用模板在基板上形成图案，使具有凹槽的模板与共聚物接触，将共聚物填充到凹槽中 的模板，导致共聚物的相分离形成具有第一段的第一相和具有第二段的第二相，从共聚物中释放模板，以及除去共聚物的第一相或第二相。
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公开(公告)号：US20100078860A1
公开(公告)日：2010-04-01
申请号：US12563461
申请日：2009-09-21
申请人： Ikuo Yoneda , Kentaro Matsunaga , Yukiko Kikuchi , Yoshihisa Kawamura , Eishi Shiobara , Shinichi Ito , Tetsuro Nakasugi , Hirokazu Kato
发明人： Ikuo Yoneda , Kentaro Matsunaga , Yukiko Kikuchi , Yoshihisa Kawamura , Eishi Shiobara , Shinichi Ito , Tetsuro Nakasugi , Hirokazu Kato
IPC分类号： B29C35/08
CPC分类号： B29C37/0003 , B29C35/0888 , B29C37/006 , B29C59/022 , B29C2035/0822 , B29C2035/0827 , B29C2059/023 , B82Y10/00 , B82Y40/00 , G03F7/0002
摘要： An imprint method includes applying a light curable resin on a substrate to be processed, the substrate including first and second regions on which the light curable resin is applied, contacting an imprint mold with the light curable resin, curing the light curable resin by irradiating the light curable resin with light passing through the imprint mold, generating gas by performing a predetermined process to the light curable resin applied on a region of the substrate, the region including at least the first region, wherein an amount of gas generated from the light curable resin applied on the first region is larger than an amount of gas generated from the light curable resin of the second region, and forming a pattern by separating the imprint mold from the light curable resin after the gas being generated.
摘要翻译： 压印方法包括将光固化树脂施加在待加工的基板上，所述基板包括施加有光固化树脂的第一和第二区域，使印模与光固化树脂接触，通过照射光固化树脂来固化光固化树脂 光固化树脂，其光通过压印模具，通过对施加在基板的区域上的光固化树脂进行预定处理产生气体，该区域至少包括第一区域，其中从光可固化 施加在第一区域上的树脂大于由第二区域的光固化树脂产生的气体的量，并且在产生气体之后通过从光固化树脂分离压印模具来形成图案。
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公开(公告)号：US06986848B2
公开(公告)日：2006-01-17
申请号：US10235496
申请日：2002-09-06
CPC分类号： G01N35/0098 , C12N15/1013 , C12Q1/6806 , G01N1/34 , G01N35/028 , C12Q2563/143
摘要： A method for purifying nucleic acids or proteins comprising a plurality of piston pumps; and a plurality of nozzles capable of having a plurality of disposable tips which are automatically attachable/detachable. The apparatus includes a mechanism for mixing a liquid mixture of each sample and a reagent in a plurality of sections of a container by sucking up a plurality of the mixtures into tips, followed by discharging the mixtures in the sections simultaneously. The apparatus also includes a mechanism for dispensing a desired amount of a reagent to be used subsequently into the same number of sections of a different container as samples, while the mixing is in progress.
摘要翻译： 一种纯化包含多个活塞泵的核酸或蛋白质的方法; 以及能够具有可自动附接/拆卸的多个一次性尖端的多个喷嘴。 该装置包括一种机构，用于通过将多个混合物吸入到尖端中，然后将该混合物同时排出，将每个样品的液体混合物和试剂混合在容器的多个部分中。 该设备还包括一种用于在混合进行期间分配期望量的试剂以随后用于与样品相同数量的不同容器的部分的机构。
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6.发明授权公开(公告)号：US06867012B2
公开(公告)日：2005-03-15
申请号：US09998130
申请日：2001-12-03
CPC分类号： C12N9/0008 , C12Q1/26 , C12Q1/32 , C12Q1/48 , Y10S435/975
摘要： The present invention provides novel glutathione-dependent formaldehyde dehydrogenase that makes possible quantitative measurement of formaldehyde by cycling reaction, and a determination method of formaldehyde and biological components, such as creatinine, creatine, and homocysteine, which produces formaldehyde as a reaction intermediate. In addition, the present invention provides a reagent kit for the above-mentioned determination method. The present invention provides a novel determination method of a homocysteine using transferase utilizing homocysteine and other compound as a pair of substrates. Particularly, the present invention provides a determination method of homocysteine which includes bringing betaine-homocysteine methyltransferase and dimethylglycine oxidase into contact with a sample and measuring produced hydrogen peroxide or formaldehyde. Moreover, the present invention provides novel dimethylglycine oxidase stable to thiol compound, which is suitably used for the measurement. The present invention provides a reagent kit used for any of the above-mentioned determination methods of homocysteine.
摘要翻译： 本发明提供了新的谷胱甘肽依赖性甲醛脱氢酶，其可以通过循环反应定量测定甲醛，以及产生甲醛作为反应中间体的甲醛和生物成分如肌酸酐，肌酸和高半胱氨酸的测定方法。 此外，本发明提供了用于上述确定方法的试剂盒。 本发明提供使用高半胱氨酸和其它化合物作为一对底物的转移酶的同型半胱氨酸的新型测定方法。 特别地，本发明提供了同型半胱氨酸的测定方法，其包括使甜菜碱 - 高半胱氨酸甲基转移酶和二甲基甘氨酸氧化酶与样品接触并测量产生的过氧化氢或甲醛。 此外，本发明提供了适用于测定的硫醇化合物稳定的新型二甲基甘氨酸氧化酶。 本发明提供了用于任何上述同型半胱氨酸测定方法的试剂盒。
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公开(公告)号：US06455684B1
公开(公告)日：2002-09-24
申请号：US09967361
申请日：2001-09-28
IPC分类号： C07H2102
CPC分类号： G01N33/582 , G01N2458/40
摘要： A method for analyzing an objective substance, comprising reacting a labeled probe with an objective substance on a biological sample, said probe comprising a label substance of the formula (I): wherein A1 is an aromatic group, R1 is a hydrogen or —COCH2COCnF2n+1 and n is an integer of 1-6, which is bonded to a probe selected from the group consisting of nucleic acid, nucleic acid binding protein, low molecular ligand and receptor for ligand (except antibody) to give a fluorescent complex, reacting the complex with an objective substance on a biological sample and assaying fluorescence of the resultant fluorescent complex, a labeled nucleic acid probe and a labeled nucleotide. According to the method of the present invention, defects such as hindrance of fluorescence due to contaminant substance, low sensitivity and the like can be resolved, thereby enabling analysis on a tissue.
摘要翻译： 一种用于分析目标物质的方法，包括使标记的探针与生物样品上的目标物质反应，所述探针包含式（I）的标记物质：其中A1为芳族基团，R1为氢或-COCH2COCnF2n + 1和n是1-6的整数，其与选自核酸，核酸结合蛋白，低分子配体和配体（抗体除外）的受体的探针结合，得到荧光复合物，使 与生物样品上的目标物质复合，并测定所得荧光复合物，标记的核酸探针和标记的核苷酸的荧光。 根据本发明的方法，可以解决诸如由于污染物质引起的荧光阻碍，低灵敏度等的缺陷，从而能够对组织进行分析。
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公开(公告)号：US06339172B1
公开(公告)日：2002-01-15
申请号：US09301406
申请日：1999-04-28
IPC分类号： C07C30900
CPC分类号： G01N33/582 , G01N2458/40
摘要： A method for analyzing an objective substance, comprsing reacting a labeled probe with an objective substance on a biological sample, said probe comprsing a label substance of the formula (I): wherein A1 is an aromatic group, R1 is a hydrogen or —COCH2COCnF2n+1 and n is an integer of 1-6, which is bonded to a probe selected from the group consisting of nucleic acid, nudeic acid binding potein, low molecular ligand and receptor for ligand (except antibody) to give a fluorescent complex, reacting the complex with an objective substance on a biolgical sample and assaying fluorescence of the resultant fluorescent complex, a labeled nucleic acid probe and a labeled nucleotide. According to the method of the present invention, defects such as hindrance of fluorescence due to contaminant substance, low sensitivity and the like can be resolved, thereby enabling analysis on a tissue.
摘要翻译： 一种用于分析目标物质的方法，包括使标记的探针与生物样品上的目标物质反应，所述探针包含式（I）的标记物质：其中A1是芳族基团，R1是氢或-COCH2COCnF2n + 1，n为1-6的整数，其与选自核酸，裸露酸结合蛋白，低分子配体和配体受体（抗体除外）的探针结合，得到荧光复合物，使 与目标物质在生物样品上复合并测定所得荧光复合物的荧光，标记的核酸探针和标记的核苷酸。 根据本发明的方法，可以解决诸如由于污染物质引起的荧光阻碍，低灵敏度等的缺陷，从而能够对组织进行分析。
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公开(公告)号：US06225065B1
公开(公告)日：2001-05-01
申请号：US09418027
申请日：1999-10-14
申请人： Masao Kitabayashi , Taku Arakawa , Hiroaki Inoue , Bunsei Kawakami , Yoshihisa Kawamura , Tadayuki Imanaka , Masahiro Takagi , Masaaki Morikawa
发明人： Masao Kitabayashi , Taku Arakawa , Hiroaki Inoue , Bunsei Kawakami , Yoshihisa Kawamura , Tadayuki Imanaka , Masahiro Takagi , Masaaki Morikawa
IPC分类号： C12Q168
CPC分类号： C12N9/1252 , C12Q1/686
摘要： A nucleic acid amplifying enzyme having a short reaction time and high fidelity is provided. The enzyme of this invention is a thermostable DNA polymerase having a nucleic acid extension rate of at least 30 bases per second and a 3′-5′ exonuclease activity. Also provided are a method and kit for amplifying nucleic acid.
摘要翻译： 提供了具有短反应时间和高保真度的核酸扩增酶。 本发明的酶是具有至少30个碱基/秒的核酸延伸速率和3'-5'核酸外切酶活性的热稳定的DNA聚合酶。 还提供了用于扩增核酸的方法和试剂盒。
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公开(公告)号：US5990302A
公开(公告)日：1999-11-23
申请号：US893561
申请日：1997-07-11
CPC分类号： C12N15/1013
摘要： A method for isolating a ribonucleic acid, which comprises dissolution of a sample containing the ribonucleic acid, such as cells, in an acidic solution containing a lithium salt and a chaotropic agent, bringing the ribonucleic acid into contact with a nucleic acid-binding carrier such as silica particles, thereby to allow selective adsorption of the ribonucleic acid alone onto said carrier, and eluting the ribonucleic acid from the nucleic acid-bound carrier; a reagent therefor; and a method for producing a cDNA from the ribonucleic acid isolated by this method. According to the present invention, a high purity ribonucleic acid can be isolated quickly and safely from a sample containing the ribonucleic acid.
摘要翻译： 一种分离核糖核酸的方法，其包括将含有核糖核酸例如细胞的样品溶解在含有锂盐和离液剂的酸性溶液中，使核糖核酸与核酸结合载体接触，如 作为二氧化硅颗粒，从而允许单核糖核酸选择性吸附到所述载体上，并从核酸结合载体洗脱核糖核酸; 一种试剂; 以及通过该方法分离的核糖核酸产生cDNA的方法。 根据本发明，可以从含有核糖核酸的样品中快速，安全地分离出高纯度的核糖核酸。
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