公开(公告)号：US20190060905A1

公开(公告)日：2019-02-28

申请号：US15977824

申请日：2018-05-11

Applicant: 10X Genomics, Inc.

Inventor： Rajiv BHARADWAJ ,  Anthony MAKAREWICZ ,  Bill Kengli LIN

IPC: B01L3/00 ,  B01F13/00

Abstract: Devices, systems, and their methods of use, for generating droplets are provided. One or more geometric parameters of a microfluidic channel can be selected to generate droplets of a desired and predictable droplet size.

公开(公告)号：US20200114358A1

公开(公告)日：2020-04-16

申请号：US16706313

申请日：2019-12-06

Applicant: 10X Genomics, Inc.

Inventor： Rajiv BHARADWAJ ,  Anthony MAKAREWICZ ,  Bill Kengli LIN

IPC: B01L3/00 ,  B01F5/04 ,  B01F11/02 ,  B01F13/00 ,  B01F3/08 ,  G01N33/543 ,  G01N33/58 ,  C12Q1/6806 ,  B01L3/02

Abstract: Devices, systems, and their methods of use, for generating droplets are provided. One or more geometric parameters of a microfluidic channel can be selected to generate droplets of a desired and predictable droplet size.

公开(公告)号：US20190060906A1

公开(公告)日：2019-02-28

申请号：US15977861

申请日：2018-05-11

Applicant: 10X Genomics, Inc.

Inventor： Rajiv BHARADWAJ ,  Anthony MAKAREWICZ ,  Bill Kengli LIN

IPC: B01L3/00 ,  B01F13/00 ,  B01F3/08

CPC classification number: B01L3/0241 ,  B01F3/0807 ,  B01F3/0811 ,  B01F5/0473 ,  B01F5/0478 ,  B01F5/048 ,  B01F11/0241 ,  B01F13/0062 ,  B01F13/0071 ,  B01L3/50273 ,  B01L3/502761 ,  B01L3/502784 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0673 ,  B01L2300/0851 ,  B01L2300/161 ,  B01L2400/086 ,  C12N2533/50 ,  C12Q1/6806 ,  C12Q1/686 ,  C12Q2563/185 ,  C40B40/06 ,  G01N33/54313 ,  G01N33/58 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2565/629

Abstract: Devices, systems, and their methods of use, for generating droplets are provided. One or more geometric parameters of a microfluidic channel can be selected to generate droplets of a desired and predictable droplet size.

公开(公告)号：US20230271187A1

公开(公告)日：2023-08-31

申请号：US18143272

申请日：2023-05-04

Applicant: 10x Genomics, Inc.

Inventor： Rajiv BHARADWAJ ,  Michael SCHNALL-LEVIN ,  Anthony MAKAREWICZ ,  Steven SHORT

IPC: B01L3/00 ,  G01N15/10 ,  G01N15/14

CPC classification number: B01L3/502761 ,  B01L3/502715 ,  B01L3/502784 ,  G01N15/1031 ,  G01N15/1056 ,  G01N15/1484 ,  B01L2200/0652 ,  B01L2200/0673 ,  B01L2300/0867 ,  B01L2400/0415 ,  B01L2400/043 ,  G01N2015/1081 ,  G01N2015/149

Abstract: Methods and systems for sorting particles are provided. Methods and systems for sorting cell beads are provided. In some cases, cell beads may be sorted from particles unoccupied with cell derivatives. In some cases, singularly occupied cell beads may be sorted from unoccupied particles and multiply occupied cell beads.

公开(公告)号：US20220080424A1

公开(公告)日：2022-03-17

申请号：US17357617

申请日：2021-06-24

Applicant: 10X Genomics, Inc.

Inventor： Ivan AKHREMICHEV ,  Rajiv BHARADWAJ ,  Lynna CHEN ,  Francis CUI ,  Rachel GERVER ,  Mohammad Rahimi LENJI ,  Bill Kengli LIN ,  Anthony MAKAREWICZ ,  Martin SAUZADE ,  Astha TANNA ,  Fernandino VALDECANAS ,  Tobias Daniel WHEELER ,  Yiran ZHANG ,  Alireza SALMANZADEH

IPC: B01L3/00 ,  B01L7/00

Abstract: Disclosed are devices, systems, kits, and methods for controlling liquid flow and, e.g., in particular, for forming droplet having substantially uniform droplet-to-droplet content. The devices, systems, and kits may include a first channel including a funnel or may include a first channel and a first-side channel, the first channel being in fluid communication with a droplet formation region. The devices, systems, and kits may further include a second channel fluidically connected to the first channel or the first side-channel. Funnels and/or side-channels may be used to enhance the control over particle spacing in the channels, thereby providing superior control over the number of particles of the same kind in formed droplets. The devices, systems, and kits of the invention may further include a mixer downstream of a channel intersection. Mixers can be used to reduce localized pockets of high concentration of dissolved ingredients.

公开(公告)号：US20200290048A1

公开(公告)日：2020-09-17

申请号：US16797727

申请日：2020-02-21

Applicant: 10X Genomics, Inc.

Inventor： Rajiv BHARADWAJ ,  Anthony MAKAREWICZ ,  Bill Kengli LIN

IPC: B01L3/00 ,  B01F11/02 ,  B01F13/00 ,  B01F3/08 ,  B01L3/02

Abstract: Devices, systems, and their methods of use, for generating droplets are provided. One or more geometric parameters of a microfluidic channel can be selected to generate droplets of a desired and predictable droplet size.

公开(公告)号：US20240253044A1

公开(公告)日：2024-08-01

申请号：US18376276

申请日：2023-10-03

Applicant: 10x Genomics, Inc.

Inventor： Rajiv BHARADWAJ ,  Anthony MAKAREWICZ ,  Bill Kengli LIN

IPC: B01L3/00 ,  B01F23/41 ,  B01F25/314 ,  B01F31/80 ,  B01F33/3011 ,  B01F33/302 ,  B01L3/02 ,  C12Q1/6806 ,  C12Q1/686 ,  C40B40/06 ,  G01N33/543 ,  G01N33/58

CPC classification number: B01L3/502784 ,  B01F23/41 ,  B01F23/4105 ,  B01F25/3141 ,  B01F25/31422 ,  B01F25/31423 ,  B01F31/84 ,  B01F33/3011 ,  B01F33/3021 ,  B01L3/0241 ,  B01L3/50273 ,  B01L3/502761 ,  C12Q1/6806 ,  G01N33/54313 ,  G01N33/58 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0673 ,  B01L2300/0851 ,  B01L2300/161 ,  B01L2400/086 ,  C12N2533/50 ,  C12Q1/686 ,  C12Q2563/185 ,  C40B40/06

Abstract: Devices, systems, and their methods of use, for generating droplets are provided. One or more geometric parameters of a microfluidic channel can be selected to generate droplets of a desired and predictable droplet size.

公开(公告)号：US20230173491A1

公开(公告)日：2023-06-08

申请号：US18161643

申请日：2023-01-30

Applicant: 10x Genomics, Inc.

Inventor： Rajiv BHARADWAJ ,  Anthony MAKAREWICZ ,  Bill Kengli LIN

IPC: B01L3/00 ,  G01N33/543 ,  B01F23/41 ,  B01F25/314 ,  B01F31/80 ,  B01F33/3011 ,  B01F33/302 ,  B01L3/02 ,  C12Q1/6806 ,  G01N33/58

CPC classification number: B01L3/502784 ,  B01L3/502761 ,  G01N33/54313 ,  B01F23/41 ,  B01F23/4105 ,  B01F25/3141 ,  B01F25/31422 ,  B01F25/31423 ,  B01F31/84 ,  B01F33/3011 ,  B01F33/3021 ,  B01L3/0241 ,  B01L3/50273 ,  C12Q1/6806 ,  G01N33/58 ,  B01L2300/0851 ,  B01L2300/161 ,  B01L2200/0652 ,  B01L2400/086 ,  B01L2200/0673 ,  B01L2200/0647 ,  C12Q1/686

Abstract: Devices, systems, and their methods of use, for generating droplets are provided. One or more geometric parameters of a microfluidic channel can be selected to generate droplets of a desired and predictable droplet size.

公开(公告)号：US20220410162A1

公开(公告)日：2022-12-29

申请号：US17851416

申请日：2022-06-28

Applicant: 10x Genomics, Inc.

Inventor： Rajiv BHARADWAJ ,  Michael SCHNALL-LEVIN ,  Anthony MAKAREWICZ ,  Steven SHORT

IPC: B01L3/00 ,  G01N15/10 ,  G01N15/14

Abstract: Methods and systems for sorting particles are provided. Methods and systems for sorting cell beads are provided. In some cases, cell beads may be sorted from particles unoccupied with cell derivatives. In some cases, singularly occupied cell beads may be sorted from unoccupied particles and multiply occupied cell beads.

公开(公告)号：US20200206742A1

公开(公告)日：2020-07-02

申请号：US16812866

申请日：2020-03-09

Applicant: 10X Genomics, Inc.

Inventor： Rajiv BHARADWAJ ,  Anthony MAKAREWICZ ,  Bill Kengli LIN

IPC: B01L3/00 ,  B01F5/04 ,  G01N33/543 ,  B01F3/08 ,  B01F13/00 ,  B01F11/02 ,  B01L3/02 ,  C12Q1/6806 ,  G01N33/58

Abstract: Devices, systems, and their methods of use, for generating droplets are provided. One or more geometric parameters of a microfluidic channel can be selected to generate droplets of a desired and predictable droplet size.