公开(公告)号：US20150107995A1

公开(公告)日：2015-04-23

申请号：US14589314

申请日：2015-01-05

Applicant: ADVANCED LIQUID LOGIC, INC.

Inventor： Ramakrishna Sista ,  Vamsee Pamula ,  Arjun Sudarsan ,  Vijay Srinivasan ,  Prasanna Thwar

IPC: B01L3/00 ,  G01N27/447

CPC classification number: B01L3/502792 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0647 ,  B01L2300/0819 ,  B01L2300/0851 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/089 ,  B01L2300/161 ,  B01L2400/0427 ,  B01L2400/043

Abstract: The invention provides droplet actuators and droplet actuator techniques. Among other things, the droplet actuators and methods are useful for manipulating beads on a droplet actuator, such as conducting droplet operations using bead-containing droplets on a droplet actuator. For example, beads may be manipulated on a droplet actuator in the context of executing a sample preparation protocol and/or an assay protocol. An output of the methods of the invention may be beads prepared for execution of an assay protocol. Another output of the methods of the invention may be results of an assay protocol executed using beads. Among the methods described herein are methods of concentrating beads in droplets, methods of washing beads, methods of suspending beads, methods of separating beads, methods of localizing beads within a droplet, methods of forming emulsions in which droplets include beads, methods of loading beads into a droplet operations gap of a droplet actuator, methods of organizing beads in a monolayer, and methods of capturing, trapping or restraining beads.

Abstract translation: 本发明提供了液滴致动器和液滴致动器技术。 除其他之外，液滴致动器和方法可用于操纵液滴致动器上的珠，例如使用液滴致动器上的含珠液滴进行液滴操作。 例如，在执行样品制备方案和/或测定方案的上下文中，珠可以在液滴致动器上操作。 本发明方法的输出可以是准备用于执行测定方案的珠粒。 本发明方法的另一个输出可以是使用珠子执行的测定方案的结果。 在本文描述的方法中，将珠粒集中在液滴中的方法，洗涤珠粒的方法，悬浮珠粒的方法，分离珠粒的方法，在液滴内定位珠粒的方法，形成液滴包括珠子的乳液的方法，加载珠粒的方法 液滴致动器的液滴操作间隙，单层组织珠粒的方法，以及捕获，捕获或抑制珠粒的方法。

公开(公告)号：US20170241949A1

公开(公告)日：2017-08-24

申请号：US15472248

申请日：2017-03-28

Applicant: Advanced Liquid Logic, Inc.

Inventor： Donovan Bort ,  Carrie Graham ,  Vamsee Pamula ,  Michael Pollack ,  Ramakrishna Sista ,  Vijay Srinivasan

IPC: G01N27/447 ,  B01F13/00 ,  B01L3/00 ,  B01F1/00

CPC classification number: G01N27/44791 ,  B01F1/0027 ,  B01F13/0071 ,  B01F13/0076 ,  B01F2001/0055 ,  B01F2215/0037 ,  B01L3/502761 ,  B01L3/502792 ,  B01L2200/0605 ,  B01L2200/0668 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/089 ,  B01L2400/0406 ,  B01L2400/0418 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0487 ,  B81B1/004

Abstract: A droplet actuator for manipulating a fluid using an electrical field includes a droplet arranged on or over an electrode. The droplet includes a set of beads arranged substantially in a monolayer on or over a surface of the droplet actuator.

公开(公告)号：US20160231268A1

公开(公告)日：2016-08-11

申请号：US15099147

申请日：2016-04-14

Applicant: ADVANCED LIQUID LOGIC, INC. ,  Duke University

Inventor： Vamsee Pamula ,  Vijay Srinivasan ,  Michael G. Pollack ,  Richard B. Fair

IPC: G01N27/26 ,  G01N27/416 ,  G01N27/327

CPC classification number: G01N27/26 ,  B01F13/0071 ,  B01F13/0076 ,  B01L3/0268 ,  B01L3/502715 ,  B01L3/502723 ,  B01L3/502761 ,  B01L3/502784 ,  B01L3/502792 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/027 ,  B01L2200/0668 ,  B01L2200/0673 ,  B01L2200/0684 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/089 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0436 ,  B01L2400/0442 ,  B01L2400/0448 ,  C12Q1/686 ,  C12Q1/6869 ,  G01N27/3271 ,  G01N27/416 ,  G01N27/447 ,  G01N35/00029 ,  G01N2035/00158 ,  G01N2035/00237

Abstract: The present invention relates to droplet-based surface modification and washing. According to one embodiment, a method of splitting a droplet is provided, the method including providing a droplet microactuator including a droplet including one or more beads and immobilizing at least one of the one or more beads. The method further includes conducting one or more droplet operations to divide the droplet to yield a set of droplets including a droplet including the one or more immobilized beads and a droplet substantially lacking the one or more immobilized beads.

公开(公告)号：US20150148238A1

公开(公告)日：2015-05-28

申请号：US14609744

申请日：2015-01-30

Applicant: Advanced Liquid Logic, Inc. ,  Duke University

Inventor： Vamsee Pamula ,  Vijay Srinivasan ,  Michael G. Pollack ,  Richard B. Fair

IPC: B01L3/00 ,  B01L7/00 ,  G01N27/447 ,  C12Q1/68

CPC classification number: G01N27/26 ,  B01F13/0071 ,  B01F13/0076 ,  B01L3/0268 ,  B01L3/502715 ,  B01L3/502723 ,  B01L3/502761 ,  B01L3/502784 ,  B01L3/502792 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/027 ,  B01L2200/0668 ,  B01L2200/0673 ,  B01L2200/0684 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/089 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0436 ,  B01L2400/0442 ,  B01L2400/0448 ,  C12Q1/686 ,  C12Q1/6869 ,  G01N27/3271 ,  G01N27/416 ,  G01N27/447 ,  G01N35/00029 ,  G01N2035/00158 ,  G01N2035/00237

Abstract: The present invention relates to droplet-based surface modification and washing. According to one embodiment, a method of splitting a droplet is provided, the method including providing a droplet microactuator including a droplet including one or more beads and immobilizing at least one of the one or more beads. The method further includes conducting one or more droplet operations to divide the droplet to yield a set of droplets including a droplet including the one or more immobilized beads and a droplet substantially lacking the one or more immobilized beads.

Abstract translation: 本发明涉及液滴表面改性和洗涤。 根据一个实施例，提供了一种分离液滴的方法，所述方法包括提供包括包含一个或多个珠并且固定所述一个或多个珠中的至少一个珠的液滴的液滴微致动器。 该方法还包括进行一个或多个液滴操作以分开液滴以产生一组液滴，包括包含一个或多个固定化珠粒的液滴和基本上不含一个或多个固定化珠粒的液滴。