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    Modified tobacco mosaic virus particles as scaffolds for display of protein antigens for vaccine applications
    5.
    发明申请
    Modified tobacco mosaic virus particles as scaffolds for display of protein antigens for vaccine applications 审中-公开

    公开(公告)号：US20090053261A1

    公开(公告)日：2009-02-26

    申请号：US11518549

    申请日：2006-09-08

    申请人： John A. Lindbo ,  Kenneth E. Palmer ,  Mark L. Smith

    发明人： John A. Lindbo ,  Kenneth E. Palmer ,  Mark L. Smith

    IPC分类号： A61K39/00 ,  C12N7/00 ,  A61P31/00

    CPC分类号： C07K14/005 ,  A61K39/12 ,  A61K39/21 ,  A61K39/385 ,  A61K2039/5258 ,  A61K2039/55566 ,  A61K2039/6075 ,  A61K2039/625 ,  C07K2319/00 ,  C07K2319/22 ,  C07K2319/60 ,  C12N7/00 ,  C12N15/8203 ,  C12N15/8257 ,  C12N15/8258 ,  C12N2710/20022 ,  C12N2710/20023 ,  C12N2710/20034 ,  C12N2740/16122 ,  C12N2740/16134 ,  C12N2770/00022

    摘要： Display of peptides or proteins in an ordered, repetitive array, such as on the surface of a virus-like particle, is known to induce an enhanced immune response relative to vaccination with the “free” protein antigen. The 2100 coat proteins comprising the rod-shaped capsid of Tobacco mosaic virus (TMV) can accommodate short peptide insertions into the primary sequence, but the display of larger protein moieties on the virion surface by genetic fusions to the capsid protein has not been possible. Since TMV lacks surface exposed residues compatible with commonly available linker chemistries, we employed a randomized library approach to introduce a reactive lysine at the externally located at the amino-terminus of the coat protein. We found that we could easily control the extent of virion conjugation and demonstrated stoichiometric biotinylation of the introduced lysine. To characterize this modular platform for the display of heterologous proteins, we bound a model antigen (streptavidin (SA)-green fluorescent protein (GFP), expressed and purified from plants) to the surface of TMV, creating a GFP-SA decorated virus particle. Rapid and quantitative determination of the level of TMV capsid decoration was accomplished by subjecting the complex to amino acid analysis and solving the family of linear equations relating the pmoles of each residue to the known amino acid composition of the complex components. We obtained a GFP-SA tetramer loading of 26%, which corresponds to display of approximately 2200 GFP moieties per intact virion. We evaluated the immunogenicity of GFP decorated virions in both mice and guinea pigs, and found augmented humoral IgG titers in both species, relative to unbound GFP-SA tetramer. In mice, we observed a detectable humoral immune response after only a single immunization with the TMV-protein complex. By demonstrating the presentation of whole proteins, this study expands the utility of TMV as a vaccine scaffold beyond that which is possible by genetic manipulation.

    INTEGRATED HANDLE AND LATCH ASSEMBLY FOR RV SCREEN AND ENTRY DOORS
    7.
    发明申请
    INTEGRATED HANDLE AND LATCH ASSEMBLY FOR RV SCREEN AND ENTRY DOORS 有权
    集成手柄和锁定装置用于RV屏幕和入口门

    公开(公告)号：US20140015260A1

    公开(公告)日：2014-01-16

    申请号：US13939659

    申请日：2013-07-11

    申请人： Barbara J. Pickar ,  Mark L. Smith

    发明人： Barbara J. Pickar ,  Mark L. Smith

    IPC分类号： E05B65/12

    CPC分类号： E05B83/44 ,  E05B63/143 ,  E05C1/14 ,  E05C7/02 ,  Y10T292/0968

    摘要： A handle assembly is provided for a dual main door and screen door assembly of an RV or trailer. The assembly includes a main door module and a screen door module. The main door module includes an outside handle which retracts the main door plunger when the doors are both closed. The screen door module includes an inside handle which retracts the main door plunger from inside the RV or trailer when both doors are closed. The inside handle also retracts the screen door plunger when from inside the RV or trailer when the screen door is closed and the main door is open. An outside handle on the screen door module retracts the screen door plunger from the outside when the main door is open and the screen door is closed.

    摘要翻译： 为RV或拖车的双主门和屏风门组件提供手柄组件。 组件包括主门模块和屏幕门模块。 主门模块包括当门都关闭时使主门柱塞缩回的外把手。 屏幕门模块包括内部把手，当两个门都关闭时，内部手柄将主门柱塞从RV或拖车内部缩回。 当门关闭并且主门打开时，内部把手还从RV或拖车内部缩回屏幕门柱塞。 当主门打开并且屏幕门关闭时，屏幕门模块上的外部手柄将屏幕门柱塞从外部收回。

    Flush-closing window for motor vehicles and method, guide track system and pin assembly therefor
    8.
    发明授权
    Flush-closing window for motor vehicles and method, guide track system and pin assembly therefor 有权
    汽车冲洗窗和方法，导轨系统和销组装

    公开(公告)号：US08091948B2

    公开(公告)日：2012-01-10

    申请号：US12110694

    申请日：2008-04-28

    申请人： Mark L. Smith ,  Todd E. Cripe ,  Bruce L. Drudge ,  Darren M. Grumm ,  Mark Mackenzie ,  Eric Wong

    发明人： Mark L. Smith ,  Todd E. Cripe ,  Bruce L. Drudge ,  Darren M. Grumm ,  Mark Mackenzie ,  Eric Wong

    IPC分类号： B60J1/00

    CPC分类号： B60J1/16 ,  B60J1/007

    摘要： A multi-pane flush closing window assembly for a vehicle that has a body enclosing a passenger compartment is secured in an opening in the vehicle body by a clamp ring fastened to the interior wall of the vehicle body. The improved window assembly includes at least one fixed sub-assembly and at least one sliding sub-assembly supported by a frame. The fixed sub-assembly has a sash, and removable fasteners extend through the peripheral edge of the sash into the interior side of the frame so that the fixed sub-assembly can be independently disassembled from the interior of the vehicle without removing the frame or the clamp ring. The sliding sub-assembly is removably secured to the frame by an improved multi-piece guide track and slide pin assembly that allows removal of the sliding sub-assembly without removing the frame. The slide pins are removably secured to opposite sides of the sliding sub-assembly.

    摘要翻译： 一种具有包围乘客舱的车身的车辆的多窗格冲洗关闭窗组件通过固定在车身内壁上的夹紧环固定在车身的开口中。 改进的窗组件包括至少一个固定子组件和由框架支撑的至少一个滑动子组件。 固定子组件具有窗框，并且可拆卸的紧固件通过窗框的周缘延伸到框架的内侧，使得固定的子组件可以独立地从车辆的内部拆卸而不移除框架或 夹环。 滑动子组件通过改进的多片导轨和滑动销组件可拆卸地固定到框架，其允许在不移除框架的情况下移除滑动子组件。 滑动销可拆卸地固定在滑动子组件的相对侧上。

    System and method for selectively providing termination power to a SCSI bus terminator from a host device
    10.
    发明授权
    System and method for selectively providing termination power to a SCSI bus terminator from a host device 失效
    用于从主机设备选择性地向SCSI总线终端器提供终端电力的系统和方法

    公开(公告)号：US5596757A

    公开(公告)日：1997-01-21

    申请号：US390749

    申请日：1995-02-16

    申请人： Mark L. Smith

    发明人： Mark L. Smith

    IPC分类号： G06F1/26 ,  G06F1/32 ,  G06F13/40

    CPC分类号： G06F1/3215 ,  G06F1/26 ,  G06F1/3253 ,  G06F13/4086 ,  H04L25/0298 ,  Y02B60/1235

    摘要： A system which determines whether one of a plurality of peripheral devices on a SCSI bus is providing termination power to a bus terminator and provides termination power from a power supply associated with a host device only when none of the peripheral devices are providing termination power. The system is further configured to only provide termination power from the host device when information signals are likely to be present on the information bus. Specifically, the system only provides termination power when the host device seeks access to the information bus to thereby limit consumption of the host device's power supply.

    摘要翻译： 一种确定SCSI总线上的多个外围设备中的一个是否向总线终端器提供终端电力并且仅在没有外围设备提供终端电力时从与主机设备相关联的电源提供终端电力的系统。 该系统还被配置为当信息信号可能存在​​于信息总线上时，仅从主机设备提供终端电力。 具体地，当主机设备寻求访问信息总线时，系统仅提供终端电力，从而限制主机设备电源的消耗。