摘要:
A bacteriocin is obtained by culturing cells of a strain of Micrococcus varians which, upon culturing in a culture medium, produces a bacteriocin which has agar well incubation inhibition test activity against at least one of Lactobacillus, Lactococcus, Streptococcus, Enterococcus, Listeria, Bacillus, Clostridia and Straphylococcus bacteria. The strain is cultured to obtain cultured cells in a concentration of from 107 to 1011 organisms per ml of the medium, and the culture medium supernatant is separated from the cultured cells to obtain the supernatant which contains the bacteriocin, which is also identified by having an amino acid sequence from SEQ ID NO: 1 or a sequence differing from SEQ ID NO: 1 by from 1 to 4 amino acids. The supernatant, as is, or a concentrate thereof or a bacteriocin product isolated from the supernatant by dehydration or otherwise isolated and purified therefrom is added to a food or cosmetic product to inhibit the growth of bacteria against which agar well incubation inhibition test activity is exhibited by the bacteriocin.
摘要翻译:通过培养变细菌微生物菌株的细胞获得细菌素,其在培养基中培养后产生细菌素,其对乳酸杆菌,乳球菌,链球菌,肠球菌,李斯特菌,芽孢杆菌等中的至少一种具有琼脂培养抑制试验活性, 梭菌和杆菌属细菌。 培养菌株以获得每ml培养基浓度为10 7至10 11个生物体的培养细胞,并从培养细胞中分离出培养基上清液,得到含有细菌素的上清液, 还通过具有来自SEQ ID NO:1的氨基酸序列或不同于SEQ ID NO:1的序列与1至4个氨基酸鉴定。 将上清液或其浓缩物或通过脱水或以其它方式分离和纯化从上清液中分离的细菌素产物加入到食品或化妆品中以抑制细菌的生长,从而可以显示出琼脂培养抑制试验活性的细菌生长 由细菌素。
摘要:
An isolated peptide which in a microorganism, and particularly in Micrococcus varians, is a signal peptide which initiates expression of a bactericide composition.
摘要:
A bacteriocin having activity against bacteria including Listeria monocytogenes is obtained by culturing cells of a strain of Micrococcus varians, particularly cells of deposited strains CNCM I-1586 and CNCM I-1587, to obtain cultured cells and a supernatant and by separating the supernatant from the cultured cells to obtain the supernatant which contains the bacteriocin which, in turn, may be isolated from the supernatant.
摘要:
Micrococcus varians strains which produce a composition having bactericidal activity, including deposited strains CNCM I-1586 and CNCM I-1587 and biologically pure cultures thereof and including also strains having nucleotide sequence SEQ ID NO:2 or including nucleotides 154 to 228 of sequence SEQ ID NO:2 and which may further include amino acid sequence SEQ ID NO:1 or amino acid sequence SEQ ID NO:3.
摘要翻译:产生具有杀菌活性的组合物的微球菌变种菌株,包括沉积菌株CNCM I-1586和CNCM I-1587及其生物纯培养物,并且还包括具有核苷酸序列SEQ ID NO:2或包含序列SEQ ID NO:2的核苷酸154至228的菌株 NO 2,并且其还可以包括氨基酸序列SEQ ID NO:1或氨基酸序列SEQ ID NO:3。
摘要:
Nucleotide fragments which encode a bacteriocin composition derived from Micrococcus varians and for preparing compositions to obtain the bacteriocin and for obtaining recombinant microorganisms. The nucleotide fragments include, in particular, nucleotides 154 to 228 and nucleotides 88 to 153 and their combination, nucleotides 88 to 228, identified in the sequence lists herein.
摘要:
A novel microorganism of the genus Bifidobacterium longum, in particular to its genomic and plasmid sequence and to a method of producing polypeptides of said Bifidobacterium, respectively. Also methods of detecting these nucleic acids or polypeptides, respectively. A data carrier is provided comprising nucleotide sequences and/or polypeptide sequences of NCC2705. In addition, the Bifidobacterium longum strain NCC2705 and also to food and pharmaceutical compositions containing said Bifidobacterium or active components thereof for the prevention and/or treatment of diarrhoea brought about by rotaviruses and pathogenic bacteria are provided.
摘要:
Recombinant strains of L. johnsonii which have capacities of surviving passage through the intestine, adhering to human intestinal cells and increasing phagocytosis of macrophages and which have a sequence of gene coding which is an L. johnsonii strain sequence of gene coding for D-lactate dehydrogenase modified so that the recombinant strain produces only L(+)-lactate.
摘要:
The present invention concerns a plasmid derived from Lactobacillus delbrueckii sp. comprising at least the restriction map of the FIG. 1 or portion(s) thereof; the recombinant vector comprising the said plasmid, at least one DNA sequence capable of replication into E. coli and/or Lc. lactis and at least one marker.The present invention concerns also the microorganism transformed by the said plasmid and/or by the said recombinant vector.
摘要:
The present invention pertains to the use of the DNA sequence of a Lactobacillus johnsonii strain, in particular to its genomic sequence for elucidating interactions of micro-organism with hosts they colonize, and moreover for elucidating the basis of probiotic properties exhibited by such strain. In addition, the present invention also relates to methods of detecting nucleic acids or polypeptides of Lactobacilli and related species, respectively. A data carrier is provided comprising nucleotide sequences and/or polypeptide sequences of La1.
摘要:
The present invention pertains to novel strains of the Bacillus subtilis group capable of fermenting beans, which are essentially devoid of any iso-valeric acid production. The present invention especially relates to novel strains of Bacillus natto, in which one or more genes involved in the biosynthetic pathway for the production of iso-valeric acids are essentially non-functional.