Bactericide compositions prepared and obtained from microccus varians
    1.
    发明授权
    Bactericide compositions prepared and obtained from microccus varians 失效
    由微球菌制备并获得的杀菌剂组合物

    公开(公告)号:US06689750B1

    公开(公告)日:2004-02-10

    申请号:US08693353

    申请日:1996-08-06

    IPC分类号: A23L33526

    摘要: A bacteriocin is obtained by culturing cells of a strain of Micrococcus varians which, upon culturing in a culture medium, produces a bacteriocin which has agar well incubation inhibition test activity against at least one of Lactobacillus, Lactococcus, Streptococcus, Enterococcus, Listeria, Bacillus, Clostridia and Straphylococcus bacteria. The strain is cultured to obtain cultured cells in a concentration of from 107 to 1011 organisms per ml of the medium, and the culture medium supernatant is separated from the cultured cells to obtain the supernatant which contains the bacteriocin, which is also identified by having an amino acid sequence from SEQ ID NO: 1 or a sequence differing from SEQ ID NO: 1 by from 1 to 4 amino acids. The supernatant, as is, or a concentrate thereof or a bacteriocin product isolated from the supernatant by dehydration or otherwise isolated and purified therefrom is added to a food or cosmetic product to inhibit the growth of bacteria against which agar well incubation inhibition test activity is exhibited by the bacteriocin.

    摘要翻译: 通过培养变细菌微生物菌株的细胞获得细菌素,其在培养基中培养后产生细菌素,其对乳酸杆菌,乳球菌,链球菌,肠球菌,李斯特菌,芽孢杆菌等中的至少一种具有琼脂培养抑制试验活性, 梭菌和杆菌属细菌。 培养菌株以获得每ml培养基浓度为10 7至10 11个生物体的培养细胞,并从培养细胞中分离出培养基上清液,得到含有细菌素的上清液, 还通过具有来自SEQ ID NO:1的氨基酸序列或不同于SEQ ID NO:1的序列与1至4个氨基酸鉴定。 将上清液或其浓缩物或通过脱水或以其它方式分离和纯化从上清液中分离的细菌素产物加入到食品或化妆品中以抑制细菌的生长,从而可以显示出琼脂培养抑制试验活性的细菌生长 由细菌素。

    Plasmid derived from Lactobacillus elbrueckii Sp.
    8.
    发明授权
    Plasmid derived from Lactobacillus elbrueckii Sp. 失效
    源自艾氏乳杆菌的质粒

    公开(公告)号:US5639644A

    公开(公告)日:1997-06-17

    申请号:US297294

    申请日:1994-08-26

    CPC分类号: C12N15/74

    摘要: The present invention concerns a plasmid derived from Lactobacillus delbrueckii sp. comprising at least the restriction map of the FIG. 1 or portion(s) thereof; the recombinant vector comprising the said plasmid, at least one DNA sequence capable of replication into E. coli and/or Lc. lactis and at least one marker.The present invention concerns also the microorganism transformed by the said plasmid and/or by the said recombinant vector.

    摘要翻译: 本发明涉及来自德氏乳杆菌(Lactobacillus delbrueckii)的质粒。 至少包括图1的限制图。 1或其一部分; 包含所述质粒的重组载体,能够复制到大肠杆菌和/或Lc中的至少一个DNA序列。 乳酸菌和至少一种标记物。 本发明还涉及由所述质粒和/或所述重组载体转化的微生物。

    La1—the genome of a lactobacillus strain
    9.
    发明授权
    La1—the genome of a lactobacillus strain 有权
    La1 - 乳杆菌菌株的基因组

    公开(公告)号:US07875421B2

    公开(公告)日:2011-01-25

    申请号:US12244581

    申请日:2008-10-02

    IPC分类号: C12Q1/00 G01N33/554

    CPC分类号: C07K14/335

    摘要: The present invention pertains to the use of the DNA sequence of a Lactobacillus johnsonii strain, in particular to its genomic sequence for elucidating interactions of micro-organism with hosts they colonize, and moreover for elucidating the basis of probiotic properties exhibited by such strain. In addition, the present invention also relates to methods of detecting nucleic acids or polypeptides of Lactobacilli and related species, respectively. A data carrier is provided comprising nucleotide sequences and/or polypeptide sequences of La1.

    摘要翻译: 本发明涉及约翰氏乳杆菌菌株的DNA序列的用途,特别是其基因组序列用于阐明微生物与其定植宿主的相互作用,此外用于阐明这种菌株表现的益生菌性质的基础。 此外,本发明还涉及分别检测乳酸杆菌和相关物种的核酸或多肽的方法。 提供包含La1的核苷酸序列和/或多肽序列的数据载体。