公开(公告)号：US07319003B2

公开(公告)日：2008-01-15

申请号：US09030571

申请日：1998-02-24

Applicant: Charles R. Cantor ,  Marek Prezetakiewiczr ,  Cassandra L. Smith ,  Takeshi Sano

Inventor： Charles R. Cantor ,  Marek Prezetakiewiczr ,  Cassandra L. Smith ,  Takeshi Sano

IPC: C12Q1/68 ,  C12N1/36 ,  C07H21/04

CPC classification number: C12Q1/6874 ,  B01J19/0046 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/0059 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00644 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00722 ,  C12Q1/6837 ,  C40B40/06 ,  C40B80/00 ,  C12Q2565/537 ,  C12Q2525/161 ,  C12Q2565/515 ,  C12Q2525/204 ,  C12Q2521/501

Abstract: This invention is directed to methods and reagents useful for sequencing nucleic acid targets utilizing sequencing by hybridization technology comprising probes, arrays of probes and methods whereby sequence information is obtained rapidly and efficiently in discrete packages. That information can be used for the detection, identification, purification and complete or partial sequencing of a particular target nucleic acid. When coupled with a ligation step, these methods can be performed under a single set of hybridization conditions. The invention also relates to the replication of probe arrays and methods for making and replicating arrays of probes which are useful for the large scale manufacture of diagnostic aids used to screen biological samples for specific target sequences. Arrays created using PCR technology may comprise probes with 5′- and/or 3′-overhangs.

Abstract translation: 本发明涉及可用于使用包括探针，探针阵列和方法的杂交技术测​​序的用于测序核酸靶的方法和试剂，从而在离散包装中快速有效地获得序列信息。 该信息可以用于特定靶核酸的检测，鉴定，纯化和完全或部分测序。 当与连接步骤相结合时，这些方法可以在单一杂交条件下进行。 本发明还涉及探针阵列的复制和用于制备和复制探针阵列的方法，所述探针阵列可用于大规模制造用于筛选特定靶序列的生物样品的诊断辅助物。 使用PCR技术产生的阵列可以包含具有5'和/或3'突出端的探针。

公开(公告)号：US06287844B1

公开(公告)日：2001-09-11

申请号：US09019966

申请日：1998-02-06

Applicant: Przemyslaw Szafranski ,  Charlene Mello ,  Takeshi Sano ,  Cassandra L. Smith ,  David L. Kaplan ,  Charles R. Cantor

Inventor： Przemyslaw Szafranski ,  Charlene Mello ,  Takeshi Sano ,  Cassandra L. Smith ,  David L. Kaplan ,  Charles R. Cantor

IPC: C12N120

CPC classification number: C07K14/36 ,  C12N15/72 ,  C12N15/74

Abstract: Compositions and methods for the control of genetically engineered organisms are described. A more effective cell suicide approach is contemplated based on the conditional expression of the lethal Streptomyces avidinii streptavidin gene. Toxicity of streptavidin is derived from its exceptionally high binding affinity for an essential prosthetic group, D-biotin. The general requirement for biotin through the living world makes streptavidin-based conditional lethal designs applicable to a broad range of containment strategies.

Abstract translation: 描述了用于控制转基因生物的组合物和方法。 基于致死性链霉菌链霉亲和素链霉亲和素基因的条件表达考虑了更有效的细胞自杀方法。 链霉抗生物素蛋白的毒性源自其对必需的假基团D-生物素的极高的结合亲和力。 生物素通过生活世界的一般要求使基于链霉亲和素的条件致命设计适用于广泛的遏制策略。

公开(公告)号：US5795714A

公开(公告)日：1998-08-18

申请号：US110691

申请日：1993-08-23

Applicant: Charles R. Cantor ,  Marek Przetakiewicz ,  Cassandra L. Smith ,  Takeshi Sano

Inventor： Charles R. Cantor ,  Marek Przetakiewicz ,  Cassandra L. Smith ,  Takeshi Sano

IPC: G01N33/53 ,  B01J19/00 ,  C07H21/04 ,  C12M1/00 ,  C12N15/09 ,  C12Q1/68 ,  C40B40/06 ,  G01N37/00

CPC classification number: C12Q1/6874 ,  B01J19/0046 ,  C12Q1/6837 ,  C40B80/00 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/0059 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00644 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00722 ,  C40B40/06

Abstract: The invention relates to the replication of probe arrays and methods for replicating arrays of probes which are useful for the large scale manufacture of diagnostic aids used to screen biological samples for specific target sequences. Arrays created using PCR technology may comprise probes with 5'- and/or 3'-overhangs.

Abstract translation: 本发明涉及用于复制探针阵列的探针阵列的复制和可用于大规模制造用于筛选特定靶序列的生物样品的诊断辅助物的方法。 使用PCR技术产生的阵列可以包含具有5'和/或3'突出端的探针。

公开(公告)号：US07179618B2

公开(公告)日：2007-02-20

申请号：US10285876

申请日：2002-11-01

Applicant: Takeshi Sano ,  Alexander N. Glazer ,  Charles R. Cantor

Inventor： Takeshi Sano ,  Alexander N. Glazer ,  Charles R. Cantor

IPC: C12N1/20 ,  C12N15/00 ,  C07K1/00 ,  C12P21/06

CPC classification number: C07H21/04 ,  C07K14/36 ,  C07K14/825 ,  C07K2319/00 ,  C07K2319/22

Abstract: Streptavidin-metallothionein chimeric proteins with biological recognition specificity in which the streptavidin moiety provides high affinity biotin binding and the metallothionein moiety provides a high affinity metal binding. The binding affinity of the streptavidin-metallothionein chimeric protein both for biotin and heavy metal ions allows specific incorporation into, conjugation with, or labelling of any biological material containing biotin with various heavy metal ions.

Abstract translation: 具有生物识别特异性的链霉亲和素 - 金属硫蛋白嵌合蛋白，其中链霉亲和素部分提供高亲和力生物素结合和金属硫蛋白部分提供高亲和力的金属结合。 链霉抗生物素蛋白 - 金属硫蛋白嵌合蛋白对生物素和重金属离子的结合亲和力允许特异性掺入，缀合或标记含有生物素与各种重金属离子的任何生物材料。

公开(公告)号：US6124129A

公开(公告)日：2000-09-26

申请号：US19966

申请日：1998-02-06

Applicant: Przemyslaw Szafranski ,  Charlene Mello ,  Takeshi Sano ,  Cassandra L. Smith ,  David L. Kaplan ,  Charles R. Cantor

Inventor： Przemyslaw Szafranski ,  Charlene Mello ,  Takeshi Sano ,  Cassandra L. Smith ,  David L. Kaplan ,  Charles R. Cantor

IPC: C07K14/36 ,  C12N1/21 ,  C12N15/72 ,  C12N15/74 ,  C12N1/20

CPC classification number: C07K14/36 ,  C12N15/72 ,  C12N15/74

Abstract: Compositions and methods for the control of genetically engineered organisms are described. A more effective cell suicide approach is contemplated based on the conditional expression of the lethal Streptomyces avidinii streptavidin gene. Toxicity of streptavidin is derived from its exceptionally high binding affinity for an essential prosthetic group, D-biotin. The general requirement for biotin through the living world makes streptavidin-based conditional lethal designs applicable to a broad range of containment strategies.

Abstract translation: 描述了用于控制转基因生物的组合物和方法。 基于致死性链霉菌链霉亲和素链霉亲和素基因的条件表达考虑了更有效的细胞自杀方法。 链霉抗生物素蛋白的毒性源自其对必需的假基团D-生物素的极高的结合亲和力。 生物素通过生活世界的一般要求使基于链霉亲和素的条件致命设计适用于广泛的遏制策略。

公开(公告)号：US20100255558A1

公开(公告)日：2010-10-07

申请号：US12435665

申请日：2009-05-05

Applicant: Christof M. Niemeyer ,  Charles R. Cantor ,  Takeshi Sano ,  Cassandra L. Smith

Inventor： Christof M. Niemeyer ,  Charles R. Cantor ,  Takeshi Sano ,  Cassandra L. Smith

IPC: C07K17/00 ,  C07K1/00 ,  C12N9/96

CPC classification number: B82Y5/00 ,  A61K47/54 ,  A61K47/55 ,  A61K47/557 ,  A61K47/6949 ,  A61K51/1268 ,  C03C17/30 ,  C03C17/3405 ,  C12Q1/6804 ,  C12Q1/682

Abstract: The invention relates to supramolecular bioconjugates and to methods for assembling and utilizing supramolecular bioconjugates. Supramolecular bioconjugates comprise a plurality of first nucleic acids and a plurality of mediators wherein each mediator comprises a second nucleic acid complementary to a sequence within said plurality of first nucleic acids. To assemble a supramolecular bioconjugate, one or more sets of bioreactive agents are coupled to the plurality of mediators, forming a plurality of bioreactive complexes. The plurality of bioreactive complexes are hybridized to the plurality of first nucleic acids to form the supramolecular bioconjugate. Bioconjugates can be used to detect and isolate targets, to screen samples for targets such as antigens, to treat patients with multiple agents or to diagnose disorders in the form of a kit.

Abstract translation: 本发明涉及超分子生物缀合物和组分和利用超分子生物缀合物的方法。 超分子生物缀合物包含多个第一核酸和多个介体，其中每个介体包含与所述多个第一核酸内的序列互补的第二核酸。 为了组装超分子生物缀合物，将一组或多组生物反应剂偶联至多个介体，形成多个生物反应性复合物。 多个生物反应性复合物与多个第一核酸杂交以形成超分子生物缀合物。 生物共轭物可用于检测和分离靶标，筛选靶标如抗原的样品，以治疗患有多种药物的患者或以试剂盒的形式诊断疾病。

公开(公告)号：US06391590B1

公开(公告)日：2002-05-21

申请号：US07780717

申请日：1991-10-21

Applicant: Takeshi Sano ,  Alexander N. Glazer ,  Charles R. Cantor

Inventor： Takeshi Sano ,  Alexander N. Glazer ,  Charles R. Cantor

IPC: C12N1562

CPC classification number: C07H21/04 ,  C07K14/36 ,  C07K14/825 ,  C07K2319/00 ,  C07K2319/22

Abstract: Streptavidin-metallothionein chimeric proteins with biological recognition specificity in which the streptavidin moiety provides high affinity biotin binding and the metallothionein moiety provides a high affinity metal binding. The binding affinity of the streptavidin-metallothionein chimeric protein both for biotin and heavy metal ions allows specific incorporation into, conjugation with, or labelling of any biological material containing biotin with various heavy metal ions.

Abstract translation: 具有生物识别特异性的链霉亲和素 - 金属硫蛋白嵌合蛋白，其中链霉亲和素部分提供高亲和力生物素结合和金属硫蛋白部分提供高亲和力的金属结合。 链霉抗生物素蛋白 - 金属硫蛋白嵌合蛋白对生物素和重金属离子的结合亲和力允许特异性掺入，缀合或标记含有生物素与各种重金属离子的任何生物材料。

公开(公告)号：US5679533A

公开(公告)日：1997-10-21

申请号：US479390

申请日：1995-06-07

Applicant: Przemyslaw Szafranski ,  Charlene M. Mello ,  Takeshi Sano ,  Kenneth A. Marx ,  Charles R. Cantor ,  David L. Kaplan ,  Cassandra L. Smith

Inventor： Przemyslaw Szafranski ,  Charlene M. Mello ,  Takeshi Sano ,  Kenneth A. Marx ,  Charles R. Cantor ,  David L. Kaplan ,  Cassandra L. Smith

IPC: C07K14/36 ,  C07K14/465 ,  C12N1/21 ,  C12N15/12 ,  C12N15/70 ,  G01N33/53

CPC classification number: C12N15/70 ,  C07K14/36 ,  C07K14/465

Abstract: The present invention relates to genetic containment systems which express a biotin-binding component that can be used for selectively destroying recombinant cells such as genetically engineered microorganisms. These systems may comprise a streptavidin or an avidin gene whose expression is controlled by a regulatable promoter. The regulatory agent such as a transcriptional effector is expressed from another gene which may also be expressed and its expression controlled by the containment system. Expression of the agent can be designed to respond to physiological changes in the environment. The invention also relates to containment systems and methods for the selective detection or tracking of recombinant cells and to eukaryotic and prokaryotic cells which contain these genetic containment systems.

公开(公告)号：US5665539A

公开(公告)日：1997-09-09

申请号：US131301

申请日：1993-10-04

Applicant: Takeshi Sano ,  Charles R. Cantor ,  Cassandra L. Smith

Inventor： Takeshi Sano ,  Charles R. Cantor ,  Cassandra L. Smith

IPC: A61K38/00 ,  A61K47/48 ,  C07K14/31 ,  C07K14/36 ,  C12Q1/68 ,  C12Q1/66

CPC classification number: C07K14/31 ,  B82Y5/00 ,  C07K14/36 ,  A61K38/00

Abstract: A novel system and method for sensitive antigen detection. The system utilizes immuno-polymerase chain reaction in which a specific biotinylated nucleic acid molecule is used as the marker. The biotinylated marker is attached to antigen-antibody complex through a streptavidin-protein A chimeric protein that possesses tight and specific binding affinity both for biotin and immunoglobulin G. A segment of the attached biotinylated marker is amplified by polymerase chain reactions with appropriate primers and the polymerase chain reaction products are detected by agarose gel electrophoresis. The method can detect any antigen and has a greater sensitivity than any existing antigen detection system.

Abstract translation: 一种用于敏感抗原检测的新型系统和方法。 该系统利用免疫聚合酶链反应，其中使用特异性生物素化的核酸分子作为标记。 生物素标记通过链霉抗生物素蛋白 - 蛋白A嵌合蛋白连接到抗原 - 抗体复合物上，该嵌合蛋白对于生物素和免疫球蛋白G都具有紧密和特异性的结合亲和力。通过与适当引物的聚合酶链反应扩增连接的生物素标记的片段，并且 通过琼脂糖凝胶电泳检测聚合酶链反应产物。 该方法可以检测任何抗原并且比任何现有的抗原检测系统具有更高的灵敏度。

公开(公告)号：US5561043A

公开(公告)日：1996-10-01

申请号：US189448

申请日：1994-01-31

Applicant: Charles R. Cantor ,  Christof M. Niemeyer ,  Cassandra L. Smith ,  Takeshi Sano ,  Donald J. Hnatowich ,  Mary Rusckowski

Inventor： Charles R. Cantor ,  Christof M. Niemeyer ,  Cassandra L. Smith ,  Takeshi Sano ,  Donald J. Hnatowich ,  Mary Rusckowski

IPC: G01N33/566 ,  A61K38/00 ,  A61K38/16 ,  A61K38/21 ,  A61K38/22 ,  A61K38/27 ,  A61K38/43 ,  A61K39/00 ,  A61K39/395 ,  A61K45/00 ,  A61K47/48 ,  A61K49/00 ,  A61K51/00 ,  A61K51/12 ,  A61P31/04 ,  A61P31/12 ,  A61P35/00 ,  A61P35/02 ,  C03C17/30 ,  C03C17/34 ,  C07H21/04 ,  C12N15/09 ,  C12Q1/68 ,  C07H21/02

CPC classification number: B82Y5/00 ,  A61K47/481 ,  A61K47/48146 ,  A61K47/48961 ,  A61K51/1268 ,  C03C17/30 ,  C03C17/3405 ,  C12Q1/6804 ,  C12Q1/682 ,  Y10S977/80 ,  Y10S977/808 ,  Y10S977/898 ,  Y10S977/906 ,  Y10S977/92

Abstract: The invention is directed to constructs and compositions containing multimeric forms of nucleic acid. Multimeric nucleic acids comprise single-stranded nucleic acids attached via biotin to streptavidin and bound with a functional group. These constructs can be utilized in vivo to treat or identify diseased tissue or cells. Repeated administrations of multimeric nucleic acid compositions produce a rapid and specific amplification of nucleic acid constructs and their attached functional groups. For treatment purposes, functional groups may be toxins, radioisotopes, genes or enzymes. Diagnostically, labeled multimeric constructs may be used to identify specific targets in vivo or in vitro. Multimeric nucleic acids may also be used in nanotechnology and to create self-assembling polymeric aggregates such as membranes of defined porosity, microcircuits and many other products.