Method for the closed-loop control of the boost pressure of a
turbocharged internal combustion engine
    2.
    发明授权
    Method for the closed-loop control of the boost pressure of a turbocharged internal combustion engine 失效
    用于涡轮增压内燃机的增压压力的闭环控制的方法

    公开(公告)号:US6067799A

    公开(公告)日:2000-05-30

    申请号:US220235

    申请日:1998-12-23

    IPC分类号: F02D41/00 F02D23/00

    CPC分类号: F02D41/0007 Y02T10/144

    摘要: The boost pressure in a turbocharged internal combustion engine is regulated by closed-loop control. A current performance graph value u(k), corresponding to a desired boost pressure, is determined from a speed/load-dependent performance graph. After that, the current performance graph value u(k) is compared with a previous performance graph value u(k-1). If the current performance graph value u(k) is greater than or equal to the previous performance graph value u(k-1), then the current performance graph value u(k) is transmitted as the process parameter x(k). Conversely, if the current performance graph value u(k) is less than the previous performance graph value u(k-1), then the current performance graph value u(k) is filtered by a PT.sub.2 member after an idle time elapses and is transmitted as the process parameter x(k).

    摘要翻译: 涡轮增压内燃机的增压压力由闭环控制来调节。 根据速度/负载相关的性能图确定对应于期望的增压压力的当前性能图值u(k)。 之后,将当前性能图值u(k)与先前的性能图值u(k-1)进行比较。 如果当前性能图值u(k)大于或等于先前的性能曲线图值u(k-1),那么当前的性能图值u(k)被作为过程参数x(k)被发送。 相反,如果当前的性能图值u(k)小于先前的性能图值u(k-1),那么在空闲时间过去之后,当前性能图值u(k)被PT2成员过滤,并且是 作为过程参数x(k)发送。

    Detection of PCR products in gel electrophoresis
    4.
    发明授权
    Detection of PCR products in gel electrophoresis 失效
    凝胶电泳检测PCR产物

    公开(公告)号:US08053213B2

    公开(公告)日:2011-11-08

    申请号:US12876320

    申请日:2010-09-07

    申请人: Christian Birkner

    发明人: Christian Birkner

    摘要: Disclosed is a method for analyzing nucleic acids in a sample comprising the following steps: (i) adding a DNA binding dye containing a benzothiazolium or benzoxazolium group to the sample to be analyzed, (ii) carrying out a polymerase chain reaction, (iii) applying the sample to a gel matrix, (iv) separating the nucleic acid molecules according to their size by applying a voltage and (v) excitation with light of a suitable wavelength for the optical visualization of the nucleic acid/DNA binding dye complexes.

    摘要翻译: 本发明公开了一种分析样品中的核酸的方法,包括以下步骤:(i)向待分析的样品中加入含有苯并噻唑鎓或苯并恶唑鎓基团的DNA结合染料,(ii)进行聚合酶链式反应,(iii) 将样品施加到凝胶基质上,(iv)通过施加电压和(v)用适当波长的光激发核酸/ DNA结合染料复合物的光学可视化,根据其大小分离核酸分子。

    NUCLEIC ACID ADSORPTION UNDER LOW-SALT CONDITIONS
    5.
    发明申请
    NUCLEIC ACID ADSORPTION UNDER LOW-SALT CONDITIONS 有权
    低盐条件下的核酸吸附

    公开(公告)号:US20090012285A1

    公开(公告)日:2009-01-08

    申请号:US12105381

    申请日:2008-04-18

    申请人: Christian Birkner

    发明人: Christian Birkner

    IPC分类号: C07H1/06 C07H1/00 C09K3/00

    CPC分类号: C12N15/1006

    摘要: Biscationic organic compounds are disclosed which promote adsorption of nucleic acids from an aqueous solution to a solid phase such as silica. Adsorption takes place under low salt conditions. Further disclosed are methods and kits suitable for nucleic acid isolation from aqueous solutions.

    摘要翻译: 揭示了一种促进核酸从水溶液吸附到固相如二氧化硅的杂质有机化合物。 吸附在低盐条件下进行。 还公开了适用于从水溶液中分离核酸的方法和试剂盒。

    Method for bisulfite treatment
    8.
    发明申请
    Method for bisulfite treatment 失效
    亚硫酸氢盐处理方法

    公开(公告)号:US20070190530A1

    公开(公告)日:2007-08-16

    申请号:US10573215

    申请日:2004-12-01

    IPC分类号: C12Q1/68 C07H19/06

    摘要: The invention is related to the detection of a methylated cytosine in a nucleic acid wherein guanidinium hydrogen sulfite is used for the preparation of a solution containing guanidinium ions and sulfite ions and subsequent modification of the nucleic acid. Thereby, a non-methylated cytosine is converted to uracil. The invention further discloses kits for performing the methods of the invention.

    摘要翻译: 本发明涉及检测核酸中的甲基化胞嘧啶,其中将亚硫酸氢胍用于制备含有胍离子和亚硫酸根离子的溶液,随后改变核酸。 由此,将非甲基化胞嘧啶转化为尿嘧啶。 本发明还公开了用于执行本发明方法的套件。

    Roofing membrane
    10.
    发明授权
    Roofing membrane 失效
    屋顶膜

    公开(公告)号:US06830802B2

    公开(公告)日:2004-12-14

    申请号:US10302355

    申请日:2002-11-22

    IPC分类号: C08L9500

    摘要: A roofing membrane, includes at least one support layer and a plurality of strips arranged in spaced-apart disposition at least on one side of the support layer and made of bituminous material and have a penetration depth between about 120 and 170 at a temperature of 50° C., and a softening point between about 100° C. and 110° C. The strips of bituminous material are separated from one another by a layer of scattered material and are covered by a fusible film. The film is composed of a plurality of strips which are oriented in parallel relationship to the bituminous strips but not interconnected.

    摘要翻译: 顶盖膜包括至少一个支撑层和多个条带,其布置成间隔布置在支撑层的至少一侧上并由沥青材料制成,并且在50℃的温度下具有介于约120和170之间的穿透深度 ℃,软化点在约100℃和110℃之间。沥青材料条通过一层分散的材料彼此分离,并被可熔膜覆盖。 该膜由多个条带组成,这些条带与烟煤条平行地定向但不相互连接。