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公开(公告)号：US20070161010A1
公开(公告)日：2007-07-12
申请号：US11435042
申请日：2006-05-16
申请人： Douglas Gjerde , Christopher Hanna , Paul Taylor , Joanne Walter , Michael Daniels , Carol Griffiths , Robert Haefele
发明人： Douglas Gjerde , Christopher Hanna , Paul Taylor , Joanne Walter , Michael Daniels , Carol Griffiths , Robert Haefele
CPC分类号： C12Q1/6827 , C12Q2565/137 , C12Q2531/113
摘要： In one aspect, a method for DNA mutation detection including the steps of PCR amplification using preferably Pho DNA polymerase, hybridization, and analysis by denaturing high performance liquid chromatography (DHPLC), the method preferably utilizing a PCR buffer and other solutions that are compatible with DHPLC analysis. In other aspects, compositions and kits including a proofreading DNA polymerase, preferably Pho DNA polymerase, and a DHPLC compatible PCR buffer are provided.
摘要翻译： 一方面，一种DNA突变检测方法，包括使用优选的Pho DNA聚合酶进行PCR扩增的步骤，通过变性高效液相色谱（DHPLC）进行杂交和分析，该方法优选利用PCR缓冲液和与 DHPLC分析。 在其它方面，提供了包括校对DNA聚合酶，优选Pho DNA聚合酶和与DHPLC相容的PCR缓冲液的组合物和试剂盒。
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公开(公告)号：US20050045543A1
公开(公告)日：2005-03-03
申请号：US10920922
申请日：2004-08-17
申请人： Douglas Gjerde , Ronald Jones , Allen Burge , Christopher Hanna , Leon Yengoyan
发明人： Douglas Gjerde , Ronald Jones , Allen Burge , Christopher Hanna , Leon Yengoyan
CPC分类号： B01J20/285 , B01D15/22 , B01J20/286 , B01J2220/54 , B01J2220/58 , B01J2220/64 , B01L3/0275 , B01L2200/0631 , B82Y30/00 , G01N1/405 , G01N30/02 , G01N30/56 , G01N2035/1053 , B01D15/3804
摘要： The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. In some embodiments, the invention provides methods of storing columns in a wet state.
摘要翻译： 本发明提供了用于从样品溶液中纯化分析物（例如生物大分子，如肽，蛋白质或核酸）的提取柱，以及制备和使用这些色谱柱的方法。 柱通常包括位于两个玻璃料之间的柱中的提取介质床。 在一些实施方案中，提取柱使用改进的移液管尖作为柱体。 在一些实施方案中，本发明提供了在湿状态下储存塔的方法。
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公开(公告)号：US20060198765A1
公开(公告)日：2006-09-07
申请号：US11367082
申请日：2006-03-02
申请人： Douglas Gjerde , Christopher Hanna , Allen Burge
发明人： Douglas Gjerde , Christopher Hanna , Allen Burge
IPC分类号： B01L3/00
CPC分类号： B01J20/26 , B01D15/424 , B01J20/265 , B01J20/28047 , B01J20/291 , B01J20/3242 , B01J2220/54 , B01L3/50255 , B01L2300/0829 , B01L2400/0487 , G01N2030/009
摘要： The invention provides a sample preparation device for processing a plurality of fluid samples comprising: (a) a plurality of sample processing chambers connected in parallel, each chamber having an internal surface and inlet and outlet ports; (b) media chambers disposed within each sample processing chamber, each media chamber comprising: (i) a bottom frit attached to and extending across the sample processing chamber; and (ii) a top barrier attached to and extending across the sample processing chamber between the bottom frit and the inlet port, wherein the top barrier, bottom frit and internal surface define a media chamber having a first average cross-sectional area; and (c) a bed of separation medium positioned inside the media chamber. In certain embodiments, the top barrier is a frit and/or the separation medium comprises gel resin beads.
摘要翻译： 本发明提供一种用于处理多个流体样品的样品制备装置，包括：（a）并联连接的多个样品处理室，每个室具有内表面和入口和出口; （b）设置在每个样品处理室内的介质室，每个介质室包括：（i）连接到并延伸穿过样品处理室的底部玻璃料; 和（ii）在底部玻璃料和入口之间附着并延伸穿过样品处理室的顶部屏障，其中顶部屏障，底部玻璃料和内部表面限定具有第一平均横截面积的介质室; 和（c）位于介质室内部的分离介质床。 在某些实施方案中，顶部阻挡层为玻璃料，和/或分离介质包含凝胶树脂珠粒。
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公开(公告)号：US20050016921A1
公开(公告)日：2005-01-27
申请号：US10920960
申请日：2004-08-17
申请人： Douglas Gjerde , Christopher Hanna
发明人： Douglas Gjerde , Christopher Hanna
CPC分类号： B01L3/0275 , B01D15/325 , B01D15/327 , B01D15/3804 , B01J20/285 , B01J20/291 , B01J2220/64 , B01L2200/0631 , B82Y30/00 , G01N30/6004 , G01N35/10 , G01N2030/009 , G01N2035/103 , G01N2035/1039 , G01N2035/1053 , Y10T436/153333 , Y10T436/25375 , Y10T436/255 , Y10T436/2575 , G01N2030/525
摘要： The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. In some embodiments, the invention provides methods characterized by the elution of analyte in a small volume of liquid.
摘要翻译： 本发明提供了用于从样品溶液中纯化分析物（例如生物大分子，如肽，蛋白质或核酸）的提取柱，以及制备和使用这些色谱柱的方法。 柱通常包括位于两个玻璃料之间的柱中的提取介质床。 在一些实施方案中，提取柱使用改进的移液管尖作为柱体。 在一些实施方案中，本发明提供了以分析物在小体积液体中洗脱为特征的方法。
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公开(公告)号：US20050019951A1
公开(公告)日：2005-01-27
申请号：US10921010
申请日：2004-08-17
申请人： Douglas Gjerde , Allen Burge , Christopher Hanna
发明人： Douglas Gjerde , Allen Burge , Christopher Hanna
IPC分类号： B01D15/32 , B01D15/38 , B01J20/285 , B01J20/291 , B01L3/02 , G01N30/00 , G01N1/10
CPC分类号： B82Y30/00 , B01D15/325 , B01D15/327 , B01D15/3804 , B01J20/285 , B01J20/291 , B01J2220/64 , B01L3/0275 , B01L2200/0631 , G01N2030/009 , G01N2035/1053 , Y10T436/25375 , Y10T436/255 , Y10T436/2575
摘要： The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. In some embodiments, the invention provides methods characterized by the use of verly slow flow rates.
摘要翻译： 本发明提供了用于从样品溶液中纯化分析物（例如生物大分子，如肽，蛋白质或核酸）的提取柱，以及制备和使用这些色谱柱的方法。 柱通常包括位于两个玻璃料之间的柱中的提取介质床。 在一些实施方案中，提取柱使用改进的移液管尖作为柱体。 在一些实施方案中，本发明提供了以使用极慢的流速为特征的方法。
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公开(公告)号：US20060202922A1
公开(公告)日：2006-09-14
申请号：US11373535
申请日：2006-03-09
申请人： Christopher Hanna , Douglas Gjerde
发明人： Christopher Hanna , Douglas Gjerde
IPC分类号： G09G3/30
CPC分类号： G01N1/405 , G01N35/1074 , G01N2035/1032
摘要： This invention relates to a method of optimizing a process for extracting an analyte from a liquid sample. A plurality of samples can be processed simultaneously while varying several factors independently to determine extraction efficiency under a wide variety of conditions.
摘要翻译： 本发明涉及优化从液体样品中提取分析物的方法。 可以同时处理多个样品，同时独立地改变几个因素，以在各种条件下确定提取效率。
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公开(公告)号：US20050258097A1
公开(公告)日：2005-11-24
申请号：US11155464
申请日：2005-06-17
申请人： Douglas Gjerde , Christopher Hanna , Jeremy Lambert , Liem Nguyen
发明人： Douglas Gjerde , Christopher Hanna , Jeremy Lambert , Liem Nguyen
IPC分类号： B01D15/08 , B01D15/38 , B01J20/285 , C07K1/18 , G01N1/40 , G01N30/00 , G01N30/06 , G01N35/10
CPC分类号： G01N1/40 , B01D15/3804 , B01J20/285 , B01J2220/54 , C07K1/18 , G01N1/405 , G01N2030/009 , G01N2030/062 , G01N2035/1053 , Y10T436/25375
摘要： The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. The invention also provides methods for using the extraction columns including methods for varying parameters, such as the volume of sample loaded, the number of capture cycles performed, and the number or wash cycles performed to achieve a particular desired result.
摘要翻译： 本发明提供了用于从样品溶液中纯化分析物（例如生物大分子，如肽，蛋白质或核酸）的提取柱，以及制备和使用这些色谱柱的方法。 柱通常包括位于两个玻璃料之间的柱中的提取介质床。 在一些实施方案中，提取柱使用改进的移液管尖作为柱体。 本发明还提供了使用提取柱的方法，其包括用于改变参数的方法，例如加载的样品的体积，进行的捕获循环的数量以及为实现特定的期望结果而执行的数量或洗涤循环。
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公开(公告)号：US20050255604A1
公开(公告)日：2005-11-17
申请号：US10920901
申请日：2004-08-17
申请人： Douglas Gjerde , Christopher Hanna , Allen Burge
发明人： Douglas Gjerde , Christopher Hanna , Allen Burge
CPC分类号： B01L3/0275 , B01D15/325 , B01D15/327 , B01D15/3804 , B01J20/285 , B01J20/291 , B01J2220/64 , B01L2200/0631 , B82Y30/00 , G01N30/6004 , G01N35/10 , G01N2030/009 , G01N2035/103 , G01N2035/1039 , G01N2035/1053 , Y10T436/153333 , Y10T436/25375 , Y10T436/255 , Y10T436/2575 , G01N2030/525
摘要： The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies.
摘要翻译： 本发明提供了用于从样品溶液中纯化分析物（例如生物大分子，如肽，蛋白质或核酸）的提取柱，以及制备和使用这些色谱柱的方法。 柱通常包括位于两个玻璃料之间的柱中的提取介质床。 在一些实施方案中，提取柱使用改进的移液管尖作为柱体。
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公开(公告)号：US20100158259A1
公开(公告)日：2010-06-24
申请号：US12619653
申请日：2009-11-16
CPC分类号： H04R5/04 , H03G3/3005 , H03G3/3089 , H03G3/341 , H04R3/007 , H04R2430/01 , H04S1/00
摘要： A disclosed system and method dynamically controls the perceived volume of a stereo audio program including left and right channel signals. The system comprises: a dynamic volume control configured and arranged so as to maintain a perceived constant volume level of the stereo audio program; and an excessive spatial processing protection processor configured and arranged for controlling the level of a difference signal, created as a function of the right channel signal subtracted from the left channel signal (L−R), relative to the level of a sum signal, created as a function of the right channel signal plus the left channel signal;; wherein the excessive spatial processing protection processor processes the audio signals so as to control the difference (L−R) signal relative to the sum (L+R) signal. A system and method are also provided for dynamically controlling the perceived volume of a stereo audio program including left and right channel signals, comprising: a dynamic volume control configured and arranged so as to maintain a perceived constant volume level of the stereo audio program; and a program change detector configured and arranged to provide a program change signal indicating that the volume of the left and right channel signals has dropped below a threshold level for at least a threshold time period so as to anticipate a possible change in the sound level of the left and right channel signals; wherein the dynamic volume control is responsive to the program change signal.
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公开(公告)号：US20060286599A1
公开(公告)日：2006-12-21
申请号：US11508582
申请日：2006-08-22
申请人： Douglas Gierde , Christopher Hanna
发明人： Douglas Gierde , Christopher Hanna
摘要： The invention provides, inter alia, methods of extracting an analyte from a solution comprising the steps of: passing a solution containing an analyte through an extraction channel having a solid phase extraction surface, whereby analyte adsorbs to the extraction surface of said extraction channel; purging bulk liquid from said extraction channel; and eluting the analyte by passing a desorption solvent through the channel. The invention further provides reagents, columns and instrumentation related to this and other methods.
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