公开(公告)号：US20210031197A1

公开(公告)日：2021-02-04

申请号：US17024221

申请日：2020-09-17

申请人： EMULATE, Inc.

发明人： S. Jordan Kerns ,  Riccardo Barrile ,  Geraldine Hamilton ,  Catherine Karalis ,  Daniel Levner ,  Carolina Lucchesi ,  Antonio Varone ,  Remi Villenave

IPC分类号： B01L3/00 ,  C12M1/00 ,  C12M3/06 ,  C12M1/42 ,  G01N33/50

摘要： An in vitro microfluidic “organ-on-chip” is described herein that mimics the structure and at least one function of specific areas of the epithelial system in vivo. In particular, a multicellular, layered, microfluidic culture is described, allowing for interactions between lamina propria-derived cells and the associated tissue specific epithelial cells and endothelial cells. This in vitro microfluidic system can be used for modeling inflammatory tissue, e.g., autoimmune disorders involving epithelia and diseases involving epithelial layers. These multicellular, layered microfluidic “organ-on-chip”, e.g. “epithelia-on-chip” further allow for comparisons between types of epithelia tissues, e.g., lung (Lung-On-Chip), bronchial (Airway-On-Chip), skin (Skin-On-Chip), cervix (Cervix-On-Chip), blood brain barrier (BBB-On-Chip), etc., in additional to neurovascular tissue, (Brain-On-Chip), and between different disease states of tissue, i.e. healthy, pre-disease and diseased areas. Additionally, these microfluidic “organ-on-chips” allow identification of cells and cellular derived factors driving disease states in addition to drug testing for reducing inflammation effecting epithelial regions.

公开(公告)号：US20210003559A1

公开(公告)日：2021-01-07

申请号：US16912391

申请日：2020-06-25

申请人： Hoffmann-La Roche Inc. ,  F. Hoffmann-La Roche AG ,  Emulate, Inc.

发明人： Cristina Bertinetti-Lapatki ,  Lauriane Cabon ,  Adrian B. Roth ,  Annie Moisan ,  Nikolche Gjorevski ,  Jordan S. Kerns ,  Geraldine A. Hamilton ,  Catherine Karalis ,  Heather Grant ,  Riccardo Barrile ,  Debora Barreiros Petropolis ,  Chaitra Belgur

IPC分类号： G01N33/50

摘要： Disclosed herein is a method for assessing a compound interacting with a target on polarized epithelial cells. The method comprising the steps of providing an organ chip comprising a main channel and polarized epithelial cells, wherein the main channel is divided into an apical channel and a basal channel separated by the polarized epithelial cells, wherein the apical side of the polarized epithelial cells is directed towards the apical channel and the basolateral side of the polarized epithelial cells is directed towards the basal channel. Determining the localization and optionally the expression level of the target on the polarized epithelial cells. Administering the compound and optionally immune cells, preferably peripheral blood mononuclear cells (PBMC) to the basal channel, when the target is localized on the basolateral side of the epithelial cells or administering the compound and optionally immune cells, preferably peripheral blood mononuclear cells (PBMC) to the apical channel, when the target is localized on the apical side of the epithelial cells. Measuring a parameter of the administration of the compound and the peripheral blood mononuclear cells.

公开(公告)号：US20240076625A1

公开(公告)日：2024-03-07

申请号：US18243242

申请日：2023-09-07

申请人： EMULATE, INC.

发明人： Antonio Varone ,  Magdalena Kasendra ,  Carolina Lucchesi ,  S. Jordan Kerns ,  Riccardo Barrile ,  Sonalee Barthakur

IPC分类号： C12N5/071 ,  B01L3/00 ,  C12M1/00 ,  C12M1/12 ,  C12M3/06 ,  G01N1/30 ,  G01N33/50

CPC分类号： C12N5/0679 ,  B01L3/502715 ,  B01L3/502761 ,  C12M23/16 ,  C12M23/26 ,  C12M25/02 ,  C12N5/069 ,  G01N1/30 ,  G01N33/5047 ,  G01N33/5064 ,  B01L2200/16 ,  B01L2300/123 ,  B01L2300/16 ,  C12N2500/00 ,  C12N2501/052 ,  C12N2501/2301 ,  C12N2501/2306 ,  C12N2501/25

摘要： The present invention contemplates compositions, devices and methods of simulating biological fluids in a fluidic device, including but not limited to a microfluidic chip. In one embodiment, fluid comprising a colloid under flow in a microfluidic chip has a fluid density or viscosity similar to a bodily fluid, e.g. blood, lymph, lung fluid, or the like. In one embodiment, a fluid is provided as a rheologically biomimetic blood surrogate or substitute for simulating physiological shear stress and cell dynamics in fluidic device, including but not limited to immune cells.

公开(公告)号：US11833512B2

公开(公告)日：2023-12-05

申请号：US17215900

申请日：2021-03-29

申请人： EMULATE, Inc.

发明人： S. Jordan Kerns ,  Riccardo Barrile ,  Geraldine Hamilton ,  Catherine Karalis ,  Daniel Levner ,  Carolina Lucchesi ,  Antonio Varone ,  Remi Villenave

IPC分类号： B01L3/00 ,  C12M1/00 ,  C12M3/06 ,  C12M1/42 ,  G01N33/50 ,  C12N5/00

CPC分类号： B01L3/502753 ,  B01L3/502715 ,  C12M23/16 ,  C12M29/04 ,  C12M35/08 ,  G01N33/5044 ,  C12N5/0018 ,  C12N5/0075

摘要： An in vitro microfluidic “organ-on-chip” is described herein that mimics the structure and at least one function of specific areas of the epithelial system in vivo. In particular, a multicellular, layered, microfluidic culture is described, allowing for interactions between lamina propria-derived cells and the associated tissue specific epithelial cells and endothelial cells. This in vitro microfluidic system can be used for modeling inflammatory tissue, e.g., autoimmune disorders involving epithelia and diseases involving epithelial layers. These multicellular, layered microfluidic “organ-on-chip”, e.g. “epithelia-on-chip” further allow for comparisons between types of epithelia tissues, e.g., lung (Lung-On-Chip), bronchial (Airway-On-Chip), skin (Skin-On-Chip), cervix (Cervix-On-Chip), blood brain barrier (BBB-On-Chip), etc., in additional to neurovascular tissue, (Brain-On-Chip), and between different disease states of tissue, i.e. healthy, pre-disease and diseased areas. Additionally, these microfluidic “organ-on-chips” allow identification of cells and cellular derived factors driving disease states in addition to drug testing for reducing inflammation effecting epithelial regions.

公开(公告)号：US10989721B2

公开(公告)日：2021-04-27

申请号：US15648162

申请日：2017-07-12

申请人： Emulate Inc.

发明人： Daniel Levner ,  Christopher David Hinojosa ,  Norman Wen ,  Jacob Fraser ,  Justin Nguyen ,  Riccardo Barrile ,  Geraldine Hamilton ,  Catherine Karalis ,  Hyoungshin Park ,  Antonio Varone ,  Andries Van der Meer ,  Monicah Otieno ,  David Conegliano

IPC分类号： G01N33/86 ,  B01L3/00 ,  C12M3/06 ,  C12M1/00 ,  C12M1/42 ,  C12M1/34 ,  G01N33/543 ,  G01N33/80

摘要： Compositions, devices and methods are described for preventing, reducing, controlling or delaying adhesion, adsorption, surface-mediated clot formation, or coagulation in a microfluidic device or chip. In one embodiment, blood (or other fluid with blood components) that contains anticoagulant is introduced into a microfluidic device comprising one or more additive channels containing one or more reagents that will re-activate the native coagulation cascade in the blood that makes contact with it “on-chip” before moving into the experimental region of the chip.

公开(公告)号：US20180015462A1

公开(公告)日：2018-01-18

申请号：US15648085

申请日：2017-07-12

申请人： Emulate Inc. ,  JANSSEN BIOTECH, Inc

发明人： Daniel Levner ,  Christopher David Hinojosa ,  Norman Wen ,  Jacob Fraser ,  Justin Nguyen ,  Riccardo Barrile ,  Geraldine Hamilton ,  Catherine Karalis ,  Hyoung Shin Park ,  Antonio Varone ,  Andries Van der Meer ,  Monica Otieno ,  David Conegliano

IPC分类号： B01L3/00 ,  G01N33/86

CPC分类号： G01N33/86 ,  B01L3/5023 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/502746 ,  B01L3/502761 ,  B01L2200/02 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2200/0647 ,  B01L2200/16 ,  B01L2300/0809 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/0883 ,  B01L2300/0887 ,  B01L2300/14 ,  B01L2300/16 ,  C12M23/16 ,  C12M29/00 ,  C12M29/04 ,  C12M29/10 ,  C12M35/08 ,  C12M41/46 ,  G01N33/54366 ,  G01N2500/10

摘要： Compositions, devices and methods are described for preventing, reducing, controlling or delaying adhesion, adsorption, surface-mediated clot formation, or coagulation in a microfluidic device or chip. In one embodiment, blood (or other fluid with blood components) that contains anticoagulant is introduced into a microfluidic device comprising one or more additive channels containing one or more reagents that will re-activate the native coagulation cascade in the blood that makes contact with it “on-chip” before moving into the experimental region of the chip.

公开(公告)号：US20170285003A1

公开(公告)日：2017-10-05

申请号：US15474214

申请日：2017-03-30

申请人： EMULATE, INC.

发明人： Geraldine A. Hamilton ,  Norman Wen ,  Catherine Karalis ,  Antonio Varone ,  Daniel Levner ,  Riccardo Barrile

IPC分类号： G01N33/50

CPC分类号： C12M23/16 ,  C12M21/00 ,  C12M25/02 ,  C12N5/0693 ,  G01N33/5011 ,  G01N33/5017

摘要： The invention generally relates to a microfluidic platforms or “chips” for testing and understanding cancer, and, more specifically, for understanding the factors that contribute to cancer invading tissues and causing metastases. Tumor cells are grown on microfluidic devices with other non-cancerous tissues under conditions that simulate tumor invasion. The interaction with immune cells can be tested to inhibit this activity by linking a cancer chip to a lymph chip.

公开(公告)号：US20240344005A1

公开(公告)日：2024-10-17

申请号：US18631480

申请日：2024-04-10

申请人： EMULATE, Inc.

发明人： Geraldine A. Hamilton ,  Norman Wen ,  Catherine Karalis ,  Antonio Varone ,  Daniel Levner ,  Riccardo Barrile

IPC分类号： C12M3/06 ,  C12M1/12 ,  C12N5/09 ,  G01N33/50

CPC分类号： C12M23/16 ,  C12M25/02 ,  C12N5/0693 ,  G01N33/5011 ,  G01N33/5017

摘要： The invention generally relates to a microfluidic platforms or “chips” for testing and understanding cancer, and, more specifically, for understanding the factors that contribute to cancer invading tissues and causing metastases. Tumor cells are grown on microfluidic devices with other non-cancerous tissues under conditions that simulate tumor invasion. The interaction with immune cells can be tested to inhibit this activity by linking a cancer chip to a lymph chip.

公开(公告)号：US12098352B2

公开(公告)日：2024-09-24

申请号：US16983850

申请日：2020-08-03

申请人： EMULATE, INC.

发明人： Janna Nawroth ,  Riccardo Barrile ,  David Conegliano ,  Remi Villenave ,  Carolina Lucchesi ,  Justin Nguyen ,  Antonio Varone ,  Catherine Karalis ,  Geraldine Hamilton

IPC分类号： C12M3/06 ,  B01L3/00 ,  C12N5/071 ,  C12N5/074

CPC分类号： C12M23/16 ,  B01L3/5027 ,  C12N5/0688 ,  C12N5/0696 ,  C12N2501/115 ,  C12N2501/117 ,  C12N2501/119 ,  C12N2501/155 ,  C12N2501/41 ,  C12N2502/1323 ,  C12N2502/27 ,  C12N2503/04 ,  C12N2506/02 ,  C12N2513/00 ,  G01N2800/12

摘要： An in vitro microfluidic “organ-on-chip” device is described herein that mimics the structure and at least one function of specific areas of the epithelial system in vivo. In particular, a stem cell-based Lung-on-Chip is described. This in vitro microfluidic system can be used for modeling differentiation of cells on-chip into lung cells, e.g., a lung (Lung-On-Chip), bronchial (Airway-On-Chip; small-Airway-On-Chip), alveolar sac (Alveolar-On-Chip), etc., for use in modeling disease states of derived tissue, i.e. as healthy, pre-disease and diseased tissues. Additionally, stem cells under differentiation protocols for deriving (producing) differentiated lung cells off-chips may be seeded onto microfluidic devices at any desired point during the in vitro differentiation pathway for further differentiation on-chip or placed on-chip before, during or after terminal differentiation.

公开(公告)号：US11970680B2

公开(公告)日：2024-04-30

申请号：US16089220

申请日：2017-03-30

申请人： EMULATE, INC.

发明人： Geraldine A. Hamilton ,  Norman Wen ,  Catherine Karalis ,  Antonio Varone ,  Daniel Levner ,  Riccardo Barrile

IPC分类号： G01N31/00 ,  C12M1/12 ,  C12M3/06 ,  C12N5/09 ,  G01N33/50 ,  G01N33/53

CPC分类号： C12M23/16 ,  C12M25/02 ,  C12N5/0693 ,  G01N33/5011 ,  G01N33/5017

摘要： The invention generally relates to a microfluidic platforms or “chips” for testing and understanding cancer, and, more specifically, for understanding the factors that contribute to cancer invading tissues and causing metastases. Tumor cells are grown on microfluidic devices with other non-cancerous tissues under conditions that simulate tumor invasion. The interaction with immune cells can be tested to inhibit this activity by linking a cancer chip to a lymph chip.