公开(公告)号：US6153192A

公开(公告)日：2000-11-28

申请号：US96044

申请日：1993-07-22

申请人： Erhard Kopetzki ,  Christian Klein ,  Dieter Mangold ,  Werner Stock ,  Reiner Schlipfenbacher

发明人： Erhard Kopetzki ,  Christian Klein ,  Dieter Mangold ,  Werner Stock ,  Reiner Schlipfenbacher

IPC分类号： A61K39/395 ,  C07K7/06 ,  C07K7/08 ,  C07K14/00 ,  C07K16/00 ,  C07K16/06 ,  C07K16/18 ,  C12N5/10 ,  C12N15/02 ,  C12P21/08 ,  C12R1/91 ,  G01N31/22 ,  G01N33/53 ,  A61K39/00 ,  A61K38/00 ,  G01N33/566

CPC分类号： C07K16/18 ,  C07K16/06 ,  Y10S530/81 ,  Y10S530/812

摘要： A peptide according to the present invention is not more than 40 amino acids long and contains a sequence which is at least 6 amino acids long from the amino acid partial sequence between the amino acids 144 and 183 of human .alpha.1-microglobulin or/and a sequence which is at least 6 amino acids long from the amino acid partial sequence between the amino acids 1 and 20 of human .alpha.1-microglobulin.An antibody according to the present invention is capable of specific binding to a peptide according to the present invention as well as to human .alpha.1-microglobulin.In order to determine human .alpha.1-microglobulin in a sample liquid by an immunoassay, the sample liquid is brought into contact simultaneously or sequentially with defined amounts of the components antibody and peptide whereby one of the components is labelled and the determination is carried out by means of this label.

摘要翻译： 根据本发明的肽不超过40个氨基酸长，并且含有与人α1-微球蛋白的氨基酸144和183之间的氨基酸部分序列至少6个氨基酸长的序列或/和 该序列与人α1-微球蛋白的氨基酸1和20之间的氨基酸部分序列至少6个氨基酸长。 根据本发明的抗体能够与根据本发明的肽以及人α1-微球蛋白特异性结合。 为了通过免疫测定法在样品液体中测定人α1-微球蛋白，将样品液体与定义量的组分抗体和肽同时或依次接触，由此其中一种组分被标记，并且测定由 该标签的手段。

公开(公告)号：US5160486A

公开(公告)日：1992-11-03

申请号：US450557

申请日：1989-12-14

申请人： Reiner Schlipfenbacher ,  Dieter Mangold ,  Rolf Lerch ,  Joachim Steinbiss

发明人： Reiner Schlipfenbacher ,  Dieter Mangold ,  Rolf Lerch ,  Joachim Steinbiss

IPC分类号： G01N33/543 ,  G01N33/558

CPC分类号： G01N33/54386 ,  G01N33/558 ,  Y10S435/805

摘要： Test carrier for analysis of a sample liquid with the help of a specific binding reaction of two bioaffine binding partners, one of which is contained in the sample and one in the reagent system of the test carrier, with several capillary-active test zones (11-16; 21-25) arranged substantially next to one another on a base layer (2). The test zones are in liquid contact with one another so that they form a liquid transport path (20, 30) along which a liquid flows, driven by capillary forces, from a start zone (11, 21), a reaction thereby taking place between the first binding partner and the reagent system containing the second reaction partner, which reaction leads to a labelled species characteristic for the desired analysis. A third binding partner specific for the first binding partner sets as capturing reagent which is so arranged in a zone of the liquid transport path (20, 30) and is so measured with regard to the amount used that it binds a part of the first binding partner and thereby removes it from the reaction leading to the characteristic labelled species. A process for the analysis of a liquid sample with the above test carrier is also provided.

摘要翻译： 借助于两个生物亚硫酸结合配偶体的特异性结合反应（其中一个包含在样品中，一个在测试载体的试剂系统中）与样品液体分离的测试载体，具有几个毛细管活性测试区（11 -16; 21-25），其基本上彼此相邻布置在基底层（2）上。 测试区彼此液体接触，使得它们形成液体输送路径（20,30），液体沿着该液体输送路径（20,30）从毛细作用力从起始区域（11,21）流动，由此发生反应 第一结合配偶体和含有第二反应伴侣的试剂系统，该反应导致所需分析的标记物质特征。 特定于第一结合伴侣的第三结合配偶体设置为捕获试剂，其被布置在液体输送路径（20,30）的区域中，并且相对于所使用的量如此测量，其结合了第一结合物的一部分 从而将其从导致特征标记物种的反应中除去。 还提供了使用上述测试载体分析液体样品的方法。

公开(公告)号：US5188939A

公开(公告)日：1993-02-23

申请号：US549893

申请日：1990-07-09

申请人： Dieter Mangold ,  Reiner Schlipfenbacher

发明人： Dieter Mangold ,  Reiner Schlipfenbacher

IPC分类号： G01N33/53 ,  G01N33/532 ,  G01N33/535 ,  G01N33/543 ,  G01N33/68

CPC分类号： G01N33/54386 ,  G01N33/535 ,  G01N33/6857 ,  Y10S436/819

摘要： A method for carrying out competitive displacement immunoassays which entails the use of an oligovalent labelled antibody having at least 4 binding sites for the analyte to be determined per label.

摘要翻译： 用于进行竞争性置换免疫测定的方法，其需要使用具有至少4个结合位点的寡核苷酸标记抗体来分析每个标签。

公开(公告)号：US5310885A

公开(公告)日：1994-05-10

申请号：US887239

申请日：1992-05-19

申请人： Josef Maier ,  Dieter Mangold ,  Reiner Schlipfenbacher

发明人： Josef Maier ,  Dieter Mangold ,  Reiner Schlipfenbacher

IPC分类号： B01J20/281 ,  C07K14/76 ,  C07K16/00 ,  C07K17/08 ,  C07K17/10 ,  G01N30/88 ,  G01N33/543 ,  C07K3/18 ,  C07K17/02 ,  C07K17/12

CPC分类号： G01N33/543 ,  Y10S530/812 ,  Y10S530/813 ,  Y10S530/814 ,  Y10S530/815 ,  Y10S530/817

摘要： The present invention provides a process for immobilizing a protein or protein containing substance. The material to be immobilized is aggregated, contacted in a liquid with a hydrophilic solid phase and the solid phase, after contact has taken place, is dried. The present invention is also concerned with the solid phase prepared by this process and with the use thereof for analytical determination.

摘要翻译： 本发明提供一种固定含有蛋白质或蛋白质的物质的方法。 要固定的材料在接触发生后被聚集，在具有亲水性固相的液体中并且固相被干燥。 本发明还涉及通过该方法制备的固相及其用于分析测定的用途。

公开(公告)号：US5403706A

公开(公告)日：1995-04-04

申请号：US383759

申请日：1989-07-20

申请人： Hans-Erich Wilk ,  Dieter Mangold ,  Rolf Lerch ,  Joachim Steinbiss

发明人： Hans-Erich Wilk ,  Dieter Mangold ,  Rolf Lerch ,  Joachim Steinbiss

IPC分类号： C12N11/08 ,  C12Q1/34 ,  G01N21/78 ,  G01N33/52 ,  G01N33/543 ,  G01N33/552 ,  C12Q1/00 ,  C12N11/06 ,  G01N33/53

CPC分类号： G01N33/525 ,  C12N11/08 ,  G01N33/54393 ,  G01N33/552 ,  Y10S530/815

摘要： A carrier matrix of polyvinyl alcohol-coated glass is dissolvably impregnated with reagent. The matrix is manufactured by slurrying glass fibers in an excess of water and polyvinyl alcohol and forming a layer, which is then dried and impregnated with reagent.

摘要翻译： 聚乙烯醇涂覆的玻璃的载体基质可溶剂地浸渍试剂。 该基体通过将玻璃纤维在过量的水和聚乙烯醇中制浆并形成一层来制造，然后将其干燥并用试剂浸渍。

公开(公告)号：US07641862B2

公开(公告)日：2010-01-05

申请号：US12330645

申请日：2008-12-09

申请人： Siegfried Noetzel ,  Jean-Philippe Bogardi ,  Dieter Mangold

发明人： Siegfried Noetzel ,  Jean-Philippe Bogardi ,  Dieter Mangold

IPC分类号： G01N1/18 ,  G01N1/10 ,  G01N33/48 ,  G01N33/72

CPC分类号： B01L3/502746 ,  B01L3/502753 ,  B01L2200/10 ,  B01L2300/0681 ,  B01L2300/0809 ,  B01L2300/0867 ,  G01N33/558 ,  G01N33/721 ,  Y10T436/25 ,  Y10T436/25125 ,  Y10T436/25375 ,  Y10T436/255 ,  Y10T436/25625 ,  Y10T436/2575

摘要： An analytical test element for blood analyses is provided having an application site and a microfluidic channel structure in fluid communication with the application site. The channel structure includes at least first and second analytical channels for receiving first and second portions of a blood sample applied to the application site. The first analytical channel includes a first analytical site to determine the total haemoglobin value (Hb) of the blood sample. The second analytical channel includes a second analytical site to determine a glycohaemoglobin value (HbA1c) of the blood sample.

摘要翻译： 提供了一种用于血液分析的分析测试元件，其具有与施用部位流体连通的施用部位和微流体通道结构。 通道结构包括至少第一和第二分析通道，用于接收施加到施用部位的血样的第一和第二部分。 第一分析通道包括用于确定血液样品的总血红蛋白值（Hb）的第一分析位点。 第二分析通道包括第二分析位点，以确定血液样品的糖血红蛋白值（HbA1c）。

公开(公告)号：US5118609A

公开(公告)日：1992-06-02

申请号：US648685

申请日：1991-01-31

申请人： Manfred Baier ,  Helmut Jering ,  Rolf Lerch ,  Dieter Mangold ,  Ellen Mossner ,  Gunter Pappert ,  Siegfried Notzel

发明人： Manfred Baier ,  Helmut Jering ,  Rolf Lerch ,  Dieter Mangold ,  Ellen Mossner ,  Gunter Pappert ,  Siegfried Notzel

IPC分类号： C07K1/16 ,  C07K16/00 ,  C07K17/10 ,  C12Q1/00 ,  C12Q1/34 ,  D04H1/54 ,  D04H1/58 ,  D21H11/12 ,  D21H13/08 ,  D21H13/16 ,  D21H13/24 ,  D21H13/26 ,  D21H17/34 ,  D21H17/36 ,  D21H17/37 ,  D21H17/39 ,  G01N33/545 ,  G01N33/548

CPC分类号： D21H17/34 ,  G01N33/545 ,  D21H11/12 ,  D21H13/08 ,  D21H13/16 ,  D21H13/24 ,  D21H13/26 ,  D21H17/36 ,  D21H17/37 ,  D21H17/39 ,  Y10S435/805 ,  Y10S435/97 ,  Y10S530/814 ,  Y10S530/815

摘要： A carrier fleece is prepared for use as reagent carrier from which reagents can be dissolved in as assay such as immunological analysis. The carrier fleece contains from 5 to 60% by weight of cellulose-containing fibers, from 40 to 95% by weight of polyester or polyamide polymer fibers or a combination thereof and from 5 to 30% by weight of the fibers of an organic binding agent which has a hydroxyl or an ester group or a combination thereof. In an immunological analysis, the carrier fleece is impregnated with an immunologically active agent such as a beta-galactosidase conjugate and then introduced into a solution of a sample containing an immunologically active substance to be analyzed. The immunologically active agent is eluted into the sample solution and the presence of the immunologically active substance in the sample is determined.

摘要翻译： 制备载体羊毛作为试剂载体，试剂可以作为试剂溶解，如免疫分析。 载体绒毛含有5至60重量％的含纤维素的纤维，40至95重量％的聚酯或聚酰胺聚合物纤维或其组合，和5至30重量％的有机粘合剂的纤维 其具有羟基或酯基或其组合。 在免疫学分析中，载体羊毛用免疫活性剂例如β-半乳糖苷酶缀合物浸渍，然后引入含有待分析的免疫活性物质的样品的溶液中。 将免疫活性剂洗脱到样品溶液中，并测定样品中免疫活性物质的存在。

公开(公告)号：US5064615A

公开(公告)日：1991-11-12

申请号：US371208

申请日：1989-06-26

申请人： Dieter Mangold ,  Brigitte Gambke

发明人： Dieter Mangold ,  Brigitte Gambke

IPC分类号： G01N31/00 ,  G01N9/36 ,  G01N21/80 ,  G01N31/22 ,  G01N33/48 ,  G01N33/52

CPC分类号： G01N31/22 ,  G01N33/52 ,  G01N33/521

摘要： The ionic strength or the specific gravity of an aqueous liquid is determined by mixing the liquid with a reagent composition which contains at least one pH buffer substance but no polyelectrolyte polymer, or at least one pH buffer substance and/or at least one complex former, determining the pH of the mixture, and evaluating the determined value.

摘要翻译： 通过将液体与含有至少一种pH缓冲物质但不含聚电解质聚合物或至少一种pH缓冲物质和/或至少一种络合物的试剂组合物混合来测定水性液体的离子强度或比重， 确定混合物的pH值，并评估确定的值。

公开(公告)号：US5015572A

公开(公告)日：1991-05-14

申请号：US340885

申请日：1989-04-19

申请人： Jurgen Backhaus ,  Dieter Mangold ,  Wolfgang-Reinhold Knappe

发明人： Jurgen Backhaus ,  Dieter Mangold ,  Wolfgang-Reinhold Knappe

IPC分类号： G01N33/48 ,  C12Q1/00 ,  C12Q1/34 ,  C12Q1/37 ,  C12Q1/44 ,  C12Q1/48 ,  G01N33/52

CPC分类号： G01N33/526 ,  C12Q1/34 ,  C12Q1/37 ,  C12Q1/48 ,  C12Q2334/50 ,  C12Q2334/70

摘要： Carrier-bound multicomponent detection system for the colorimetric determination of esterolytically- and/or proteolytically-active ingredients of body fluids, wherein the components of the detection system are present in different reagent layers, and where a liquid exchange between the layers is possible.

摘要翻译： 用于比色测定体液的酯解和/或蛋白水解活性成分的载体结合多组分检测系统，其中检测系统的组分存在于不同的试剂层中，并且层之间的液体交换是可能的。

公开(公告)号：US20110194980A1

公开(公告)日：2011-08-11

申请号：US13089721

申请日：2011-04-19

申请人： Juergen Klepp ,  Thomas Fischer ,  Rolf Lerch ,  Dieter Mangold ,  Juergen Schaeffler

发明人： Juergen Klepp ,  Thomas Fischer ,  Rolf Lerch ,  Dieter Mangold ,  Juergen Schaeffler

IPC分类号： G01N30/00

CPC分类号： B01D69/12 ,  B01D67/0088 ,  G01N33/521 ,  Y10T436/2575

摘要： A method for applying reactive films containing solids to microporous membranes is provided wherein the membrane is firstly moistened and the reactive film containing solids is applied to the membrane while it is still moist. Membranes produced in this manner can contain reactive films having a high proportion of film openers and can be used advantageously in diagnostic elements for detecting constituents and, in particular, large hydrophobic analytes in body fluids.

摘要翻译： 提供了一种将含有固体的反应性膜施加到微孔膜的方法，其中首先润湿膜，并且将含有固体的反应性膜施加到膜上，同时其仍然是潮湿的。 以这种方式生产的膜可以含有具有高比例的开膜剂的反应性膜，并且可以有利地用于检测成分的诊断元件，特别是用于体液中的大的疏水性分析物。