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    Dose determination for inducing microcavitation in retinal pigment epithelium (RPE)
    3.
    发明授权
    Dose determination for inducing microcavitation in retinal pigment epithelium (RPE) 有权
    用于诱导视网膜色素上皮（RPE）微量化的剂量测定

    公开(公告)号：US08968280B2

    公开(公告)日：2015-03-03

    申请号：US13145605

    申请日：2010-01-22

    申请人： Charles P. Lin ,  Clemens Alt ,  Ho Lee

    发明人： Charles P. Lin ,  Clemens Alt ,  Ho Lee

    IPC分类号： A61B18/18 ,  A61F9/008

    CPC分类号： A61F9/008 ,  A61F9/00821 ,  A61F2009/00863

    摘要： Methods and systems for controlling selective targeting of retinal pigment epithelium (RPE) cells within a treatment region of the RPE. The methods include (a) depositing a selected amount of energy on a test region of the RPE; (b) determining an extent to which microcavitation has occurred in the test region; and (c) on the basis of the determination, either depositing the selected amount of energy on the treatment region, or depositing an increased amount of energy on the test region, and repeating steps (b) and (c).

    摘要翻译： 用于控制RPE治疗区域内视网膜色素上皮细胞（RPE）细胞选择性靶向的方法和系统。 所述方法包括（a）在RPE的测试区域上沉积选定量的能量; （b）确定在测试区域发生微气蚀的程度; 和（c）基于该确定，将所选择的能量沉积在处理区域上，或者在测试区域上沉积增加的能量，并重复步骤（b）和（c）。

    In vivo flow cytometry based on cellular autofluorescence
    4.
    发明授权
    In vivo flow cytometry based on cellular autofluorescence 有权
    基于细胞自体荧光的体内流式细胞术

    公开(公告)号：US08574859B2

    公开(公告)日：2013-11-05

    申请号：US12867141

    申请日：2009-03-27

    申请人： Charles P. Lin ,  Alicia L. Carlson ,  Clemens Alt ,  David P. Biss ,  Costas M. Pitsillides ,  Chunqiang Li

    发明人： Charles P. Lin ,  Alicia L. Carlson ,  Clemens Alt ,  David P. Biss ,  Costas M. Pitsillides ,  Chunqiang Li

    IPC分类号： G01N33/00

    CPC分类号： A61B5/0071 ,  A61B5/0059 ,  A61B5/0068 ,  G01N15/1434 ,  G01N33/537 ,  G01N33/56972 ,  G01N2015/008 ,  G01N2015/1497 ,  Y10T436/13

    摘要： The present invention generally provides methods and systems for performing in vivo flow cytometry by using blood vessels as flow chambers through which flowing cells can be monitored in a live subject in vivo without the need for withdrawing a blood sample. In some embodiments, one or more blood vessels are illuminated with radiation so as to cause a multi-photon excitation of an exogenous fluorophore that was previously introduced into the subject to label one or more cell types of interest. In some other embodiments, rather than utilizing an exogenous fluorophore, endogenous (intrinsic) cellular fluorescence can be employed for in vivo flow cytometry. The emission of fluorescence radiation from such fluorophores in response to the excitation can be detected and analyzed to obtain information regarding a cell type of interest.

    摘要翻译： 本发明通常提供了通过使用血管作为流动室进行体内流式细胞术的方法和系统，通过该流室可以在活体内体内监测流动细胞，而不需要抽出血样。 在一些实施方案中，用辐射照射一个或多个血管，以便引起先前引入对象的外源性荧光团的多光子激发以标记一种或多种感兴趣的细胞类型。 在一些其它实施方案中，不是利用外源荧光团，内源（内在）细胞荧光可用于体内流式细胞术。 可以检测和分析来自这种荧光团的响应于激发的荧光辐射的发射以获得关于感兴趣的细胞类型的信息。

    In-vivo monitoring of circulating apoptotic cells
    5.
    发明授权
    In-vivo monitoring of circulating apoptotic cells 有权
    循环凋亡细胞的体内监测

    公开(公告)号：US07745155B2

    公开(公告)日：2010-06-29

    申请号：US11190729

    申请日：2005-07-27

    申请人： Charles P. Lin ,  Xunbin Wei ,  Dorothy Sipkins

    发明人： Charles P. Lin ,  Xunbin Wei ,  Dorothy Sipkins

    IPC分类号： G01N33/574 ,  G01N21/00 ,  G01N21/66 ,  A61K49/00

    CPC分类号： A61K49/0032 ,  A61K49/0056 ,  A61K49/0097 ,  G01N33/56966 ,  G01N33/57496 ,  G01N33/582 ,  G01N2510/00 ,  G01N2800/52 ,  Y10T436/101666 ,  Y10T436/13

    摘要： The present invention provides methods and systems for performing in vivo flow cytometry. In one embodiments, selected circulating cells of interest of a subject are labeled with fluorescent probe molecules. The labeled cells are irradiated in-vivo so as to excite the fluorescent probes, and the radiation emitted by the excited probes is detected, preferably confocally. The detected radiation is then analyzed to derive desired information, such as relative cell count, of the cells of interest. In some embodiments, the circulating cells comprise apoptotic cells whose detection can allow, e.g., non-invasive monitoring of the efficacy of a cancer treatment, such as an anti-tumor or an anti-angiogenic therapy.

    摘要翻译： 本发明提供用于进行体内流式细胞术的方法和系统。 在一个实施方案中，用荧光探针分子标记受试者感兴趣的选定的循环细胞。 将标记的细胞体内照射以激发荧光探针，并且检测被激发的探针发射的辐射，优选共方。 然后分析所检测的辐射以获得感兴趣的细胞的所需信息，例如相对细胞计数。 在一些实施方案中，循环细胞包含凋亡细胞，其检测可以允许例如非侵入性监测癌症治疗（例如抗肿瘤或抗血管生成治疗）的功效。

    Fiber-coupled multiplexed confocal microscope
    6.
    发明授权
    Fiber-coupled multiplexed confocal microscope 失效
    光纤耦合多重共焦显微镜

    公开(公告)号：US07292390B2

    公开(公告)日：2007-11-06

    申请号：US10830905

    申请日：2004-04-22

    申请人： Charles P. Lin ,  Robert H. Webb

    发明人： Charles P. Lin ,  Robert H. Webb

    IPC分类号： G02B21/06

    CPC分类号： G02B21/0032 ,  G02B21/0024 ,  G02B21/0028

    摘要： A confocal microscope system that is inherently fiberoptic compatible is described which has line scanning aided image formation. An incoherent fiberoptic bundle maps a line illumination pattern into a dispersible group of separate sources, and then remaps this confocally selected remitted light to the original line. Fibers, not confocal with the illumination, carry light to be rejected from the image back on itself upon double passing, while separate fibers carry light from non-confocal sample planes. The transformation allows efficient rejection of unwanted photons at a slit aperture. The fiber bundle and an objective lens provide a flexible probe for imaging internal tissue for pathological examination on a cellular level.

    摘要翻译： 描述了固有地光纤相容的共焦显微镜系统，其具有线扫描辅助图像形成。 不相干的光纤束将线照明图案映射成可分散的一组源，然后将该共同选择的汇入光重新映射到原始行。 不共焦照明的光纤在双重通过时将光从图像中反射回来，而分离的光纤携带非共焦样品平面的光。 该转换允许在狭缝孔径处有效地排除不需要的光子。 纤维束和物镜提供用于成像内部组织以在细胞水平进行病理检查的柔性探针。

    OPTICAL DEVICES AND METHODS FOR SELECTIVE AND CONVENTIONAL PHOTOCOAGULATION OF THE RETINAL PIGMENT EPITHELIUM
    7.
    发明申请
    OPTICAL DEVICES AND METHODS FOR SELECTIVE AND CONVENTIONAL PHOTOCOAGULATION OF THE RETINAL PIGMENT EPITHELIUM 有权
    用于选择性和常规色素沉积的光学设备和方法

    公开(公告)号：US20120296320A1

    公开(公告)日：2012-11-22

    申请号：US13480955

    申请日：2012-05-25

    申请人： Charles P. Lin ,  Clemens Alt

    发明人： Charles P. Lin ,  Clemens Alt

    IPC分类号： A61F9/008

    CPC分类号： A61F9/008 ,  A61B18/20 ,  A61B2017/00057 ,  A61B2018/00636 ,  A61F9/00821 ,  A61F2009/00863 ,  A61F2009/00897

    摘要： The present invention provides devices and methods for applying radiation to the retina of a patient. In one embodiment, an apparatus includes a radiation source for generating a radiation beam suitable for absorption by retinal pigment epithelial cells. One or more optical components are included to direct the beam onto the retina. A scanner is optically coupled to the radiation source to control movement of the beam in two dimensions to allow a scan over the retina. A controller applies control signals to the scanner to adjust beam movement to illuminate a plurality of retinal locations in a temporal sequence according to a predefined pattern. The device can be operated in one mode to effect selective targeting of retinal pigment epithelial cells, or in another mode to effect thermal photocoagulation of the retina.

    摘要翻译： 本发明提供了用于向患者的视网膜施加辐射的装置和方法。 在一个实施例中，一种装置包括用于产生适于由视网膜色素上皮细胞吸收的辐射束的辐射源。 包括一个或多个光学部件以将光束引导到视网膜上。 扫描仪光耦合到辐射源以控制光束在两个维度上的移动，以允许在视网膜上进行扫描。 控制器将控制信号施加到扫描仪，以根据预定义的模式以时间顺序调整光束移动以照亮多个视网膜位置。 该装置可以以一种模式操作以实现视网膜色素上皮细胞的选择性靶向，或在另一模式下进行视网膜的热凝固。

    Optical devices and methods for selective and conventional photocoagulation of the retinal pigment epithelium
    8.
    发明授权
    Optical devices and methods for selective and conventional photocoagulation of the retinal pigment epithelium 有权
    用于视网膜色素上皮的选择性和常规光凝的光学装置和方法

    公开(公告)号：US08187257B2

    公开(公告)日：2012-05-29

    申请号：US11263677

    申请日：2005-10-31

    申请人： Charles P. Lin ,  Clemens Alt

    发明人： Charles P. Lin ,  Clemens Alt

    IPC分类号： A61B18/20

    CPC分类号： A61F9/008 ,  A61B18/20 ,  A61B2017/00057 ,  A61B2018/00636 ,  A61F9/00821 ,  A61F2009/00863 ,  A61F2009/00897

    摘要： The present invention provides devices and methods for applying radiation to the retina of a patient. In one embodiment, an apparatus includes a radiation source for generating a radiation beam suitable for absorption by retinal pigment epithelial cells. One or more optical components are included to direct the beam onto the retina. A scanner is optically coupled to the radiation source to control movement of the beam in two dimensions to allow a scan over the retina. A controller applies control signals to the scanner to adjust beam movement to illuminate a plurality of retinal locations in a temporal sequence according to a predefined pattern. The device can be operated in one mode to effect selective targeting of retinal pigment epithelial cells, or in another mode to effect thermal photocoagulation of the retina.

    摘要翻译： 本发明提供了用于向患者的视网膜施加辐射的装置和方法。 在一个实施例中，一种装置包括用于产生适于由视网膜色素上皮细胞吸收的辐射束的辐射源。 包括一个或多个光学部件以将光束引导到视网膜上。 扫描仪光耦合到辐射源以控制光束在两个维度上的移动，以允许在视网膜上进行扫描。 控制器将控制信号施加到扫描仪，以根据预定义的模式以时间顺序调整光束移动以照亮多个视网膜位置。 该装置可以以一种模式操作以实现视网膜色素上皮细胞的选择性靶向，或在另一模式下进行视网膜的热凝固。

    DOSE DETERMINATION FOR INDUCING MICROCAVITATION IN RETINAL PIGMENT EPITHELIUM (RPE)
    9.
    发明申请
    DOSE DETERMINATION FOR INDUCING MICROCAVITATION IN RETINAL PIGMENT EPITHELIUM (RPE) 有权
    诱导上皮细胞（RPE）中微量滴定的剂量测定

    公开(公告)号：US20120029490A1

    公开(公告)日：2012-02-02

    申请号：US13145605

    申请日：2010-01-22

    申请人： Charles P. Lin ,  Clemens Alt ,  Ho Lee

    发明人： Charles P. Lin ,  Clemens Alt ,  Ho Lee

    IPC分类号： A61F9/008

    CPC分类号： A61F9/008 ,  A61F9/00821 ,  A61F2009/00863

    摘要： Methods and systems for controlling selective targeting of retinal pigment epithelium (RPE) cells within a treatment region of the RPE. The methods include (a) depositing a selected amount of energy on a test region of the RPE; (b) determining an extent to which microcavitation has occurred in the test region; and (c) on the basis of the determination, either depositing the selected amount of energy on the treatment region, or depositing an increased amount of energy on the test region, and repeating steps (b) and (c).

    摘要翻译： 用于控制RPE治疗区域内视网膜色素上皮细胞（RPE）细胞选择性靶向的方法和系统。 这些方法包括（a）在RPE的测试区域上沉积选定量的能量; （b）确定在测试区域发生微气蚀的程度; 和（c）基于该确定，将所选择的能量沉积在处理区域上，或者在测试区域上沉积增加的能量，并重复步骤（b）和（c）。

    Methods for in-vivo flow cytometry
    10.
    发明授权
    Methods for in-vivo flow cytometry 有权
    体内流式细胞仪的方法

    公开(公告)号：US08108031B2

    公开(公告)日：2012-01-31

    申请号：US11895702

    申请日：2007-08-27

    申请人： Irene Georgakoudi ,  Charles P. Lin

    发明人： Irene Georgakoudi ,  Charles P. Lin

    IPC分类号： A61B8/14

    CPC分类号： A61B5/1455 ,  A61B2503/40 ,  G01N15/1459 ,  G01N2015/0073 ,  G01N2015/008 ,  G01N2015/0084 ,  G01N2015/1486 ,  G01N2015/1488

    摘要： The present invention provides methods and systems for performing in-vivo flow cytometry to obtain desired information regarding one or more cell types of interest flowing through a subject's circulatory system. In one embodiment of the invention, a portion of the subject's circulating blood is illuminated with radiation having multiple wavelength components, and the backscattered radiation generated in response to the excitation radiation is detected at a plurality of scattering angles and analyzed to derive the desired information.

    摘要翻译： 本发明提供用于进行体内流式细胞术以获得关于流过对象的循环系统的一种或多种感兴趣细胞类型的期望信息的方法和系统。 在本发明的一个实施例中，用具有多个波长分量的辐射照射受试者的循环血液的一部分，并且以多个散射角度检测响应于激发辐射而产生的反向散射辐射并分析以导出所需信息。