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19 条记录 (耗时 0.042 秒)

    Enzyme, its method of production and its application to the preparation of methyl N-(L-aspartyl-1) L-phenylalaninate
    1.
    发明授权
    Enzyme, its method of production and its application to the preparation of methyl N-(L-aspartyl-1) L-phenylalaninate 失效
    酶，其制备方法及其在制备N-（L-天冬氨酰基-1）L-苯丙氨酸甲酯中的应用

    公开(公告)号：US4916062A

    公开(公告)日：1990-04-10

    申请号：US921632

    申请日：1986-10-21

    申请人： Francois Paul ,  Francis Duchiron ,  Pierre Monsan

    发明人： Francois Paul ,  Francis Duchiron ,  Pierre Monsan

    IPC分类号： C12P21/00 ,  C12N9/52 ,  C12R1/265

    CPC分类号： C12N9/52 ,  Y10S435/859

    摘要： A polymeric enzyme having a molecular weight higher than or equal to 200,000 and not exhibiting any proteolytic activity on casein derived from a culture of Micrococcus caseolyticus is disclosed, as is a method of producing the enzyme by filtration on gel buffered with an aqueous buffer of an enzymatic system prepared by lyophilization of an aqueous concentrate formed by ultrafiltration of the solution obtained by dissolving, in an aqueous solution of calcium chloride, an enzymatic system precipitated from a supernatant of a micro-organism culture broth by ammonium sulphate, wherein the enzyme is not retained on a gel calibrated for proteins with a molecular weight of between 5,000 and 20,000, buffered with a 20 mM TRIS-HCl buffer, at pH 7.5, containing 1.5 mM calcium chloride, and the enzymatic system is derived from a culture broth of said Micrococcus caseolyticus. The enzyme may be used for the production of aspartame by condensation of L-aspartic acid with methyl DL-phenylalaninate.

    摘要翻译： 公开了一种分子量高于或等于20万并且不表现出任何蛋白水解活性的聚合酶，所述酪蛋白衍生自小麦球菌的微生物溶菌素的培养物，以及通过在用缓冲液 将通过将通过将通过将硫酸铵从微生物培养液的上清液沉淀的酶系统溶解在氯化钙水溶液中而获得的溶液进行超滤形成的水性浓缩物冷冻干燥制备的酶系统，其中所述酶不是 保留在用于分子量为5,000至20,000的蛋白质的凝胶上校准，用含有1.5mM氯化钙的pH7.5的20mM TRIS-HCl缓冲液缓冲，酶系统来源于所述微球菌的培养液 病毒溶菌 该酶可以通过L-天冬氨酸与DL-苯丙氨酸甲酯的缩合而用于生产天冬甜素。

    Process for the purification of dextran-sucrase
    2.
    发明授权
    Process for the purification of dextran-sucrase 失效
    葡聚糖蔗糖酶纯化方法

    公开(公告)号：US4591563A

    公开(公告)日：1986-05-27

    申请号：US606642

    申请日：1984-05-03

    申请人： Francois Paul ,  Pierre Monsan ,  Daniel Auriol

    发明人： Francois Paul ,  Pierre Monsan ,  Daniel Auriol

    IPC分类号： C12N9/10 ,  C12R1/01

    CPC分类号： C12N9/1051 ,  Y10S435/814 ,  Y10S435/816 ,  Y10S435/822

    摘要： The invention is directed to a process for the purification of the dextran-sucrase produced by Leuconostoc mesenteroides bacteria.The process according to the invention consists of adding to the culture medium, that contains the extra-cellular enzyme and dextran, a quantity of a PEG type polyether so that, in the medium appear two non miscible phases, that are thus maintained under stirring in order to obtain a good contact. Thereafter the lower dextran phase is separated from the upper polyether phase in order to provide a dextran-sucrase enriched enzymatic preparation.The purified enzyme can be used in the synthesis processes of the dextrans possessing specific molecular weights for certain applications.

    摘要翻译： 本发明涉及纯化由明串珠菌肠球菌细菌产生的葡聚糖 - 蔗糖酶的方法。 根据本发明的方法包括向含有细胞外酶和葡聚糖的培养基中加入一定数量的PEG型聚醚，使得在培养基中出现两个不可混溶的相，从而保持在搅拌下 为了获得良好的接触。 此后，将较低葡聚糖相与上部聚醚相分离，以提供富含葡聚糖 - 蔗糖酶的酶制剂。 纯化的酶可用于某些应用中具有特定分子量的葡聚糖的合成过程。

    Method for the Identification of a Metabolic Pathway Family by Means of Positive Selection
    8.
    发明申请
    Method for the Identification of a Metabolic Pathway Family by Means of Positive Selection 审中-公开
    通过正选择鉴定代谢途径家族的方法

    公开(公告)号：US20100323390A1

    公开(公告)日：2010-12-23

    申请号：US12872280

    申请日：2010-08-31

    申请人： Pierre Monsan ,  Claude Bensoussan ,  Philippe Reulet ,  Renaud Nalin ,  Patrick Robe ,  Karine Tuphile ,  Benjamin Gillet ,  Pierre Pujic

    发明人： Pierre Monsan ,  Claude Bensoussan ,  Philippe Reulet ,  Renaud Nalin ,  Patrick Robe ,  Karine Tuphile ,  Benjamin Gillet ,  Pierre Pujic

    IPC分类号： C12Q1/02 ,  C40B30/06 ,  C12P33/02 ,  C12P7/22 ,  C12P7/42 ,  C12P13/02

    CPC分类号： C12P7/40 ,  C12N2503/02 ,  C12P7/02 ,  C12P13/02 ,  C12P33/00

    摘要： The invention relates to the direct selection of metabolic pathways having a determined function in the transformation of a substrate {Ai} into a target product {B}, which is of interest in the industrial, pharmaceutical or agri-food sectors. More specifically, the invention relates to the detection, within metagenomic libraries, of novel biosynthesis pathways involved in a biochemical reaction having a known product {B}. The selection and characterisation of said novel metabolic pathways enables {B} to be produced enzymatically. The invention provides an alternative to the chemical synthesis of the molecule in question {B}. Moreover, and above all, the invention can be used specifically to target and exploit the only metabolic pathways enabling the transformation of {Ai} into {B}, while eliminating the associated metabolic pathways that can catabolise the target product {B}.

    摘要翻译： 本发明涉及在将底物{Ai}转化为目标产物{B}中具有确定功能的代谢途径的直接选择，其在工业，制药或农业食品领域是感兴趣的。 更具体地，本发明涉及在宏基因组文库中检测参与具有已知产物{B}的生化反应的新生物合成途径。 所述新型代谢途径的选择和表征使得{B}可以酶促产生。 本发明提供了所述分子的化学合成（B）的替代方案。 此外，最重要的是，本发明可以专门用于靶向和利用唯一能够将{Ai}转化为{B}的代谢途径，同时消除可能分解目标产物{B}的相关代谢途径。