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    Methods and Algorithms For Cell Enumeration in a Low-Cost Cytometer
    1.
    发明申请
    Methods and Algorithms For Cell Enumeration in a Low-Cost Cytometer 有权
    低成本细胞计数器细胞计数的方法和算法

    公开(公告)号：US20110052037A1

    公开(公告)日：2011-03-03

    申请号：US12707578

    申请日：2010-02-17

    申请人： Frank A.W. Coumans ,  Jan Greve ,  Frank P. Modica ,  Leon W.M.M. Terstappen ,  Arjan G.J. Tibbe ,  John A. Verrant

    发明人： Frank A.W. Coumans ,  Jan Greve ,  Frank P. Modica ,  Leon W.M.M. Terstappen ,  Arjan G.J. Tibbe ,  John A. Verrant

    IPC分类号： G06K9/00

    CPC分类号： G01N15/1475 ,  G01N33/5094 ,  G01N33/54333

    摘要： The enumeration of cells in fluids by flow cytometry is widely used across many disciplines such as assessment of leukocyte subsets in different bodily fluids or of bacterial contamination in environmental samples, food products and bodily fluids. For many applications the cost, size and complexity of the instruments prevents wider use, for example, CD4 analysis in HIV monitoring in resource-poor countries. The novel device, methods and algorithms disclosed herein largely overcome these limitations. Briefly, all cells in a biological sample are fluorescently labeled, but only the target cells are also magnetically labeled. In addition, non-magnetically labeled cells are imaged for viability in a modified slide configuration. The labeled sample, in a chamber or cuvet, is placed between two wedge-shaped magnets to selectively move the magnetically labeled cells to the observation surface of the cuvet. An LED illuminates the cells and a CCD camera captures the images of the fluorescent light emitted by the target cells. Image analysis performed with a novel algorithm provides a count of the cells on the surface that can be related to the target cell concentration of the original sample. The compact cytometer system provides a rugged, affordable and easy-to-use technique, which can be used in remote locations.

    摘要翻译： 通过流式细胞术对流体细胞的计数被广泛应用于许多学科，例如评估不同体液中的白细胞亚群或环境样品，食品和体液中的细菌污染。 对于许多应用，仪器的成本，尺寸和复杂性阻止了更广泛的使用，例如资源贫乏国家的艾滋病毒监测中的CD4分析。 本文公开的新颖的装置，方法和算法在很大程度上克服了这些限制。 简言之，生物样品中的所有细胞都被荧光标记，但只有靶细胞也被磁性标记。 另外，非磁性标记的细胞在修改的载玻片配置中的存活力被成像。 将标记的样品置于室或铜管中，放置在两个楔形磁体之间，以选择性地将磁性标记的细胞移动到铜管的观察表面。 LED照亮细胞，CCD相机捕获由靶细胞发射的荧光的图像。 用新算法进行的图像分析提供了与原始样品的靶细胞浓度相关的表面上的细胞计数。 紧凑型细胞仪系统提供坚固，经济实惠且易于使用的技术，可用于偏远地区。

    Methods and algorithms for cell enumeration in a low-cost cytometer
    2.
    发明授权
    Methods and algorithms for cell enumeration in a low-cost cytometer 有权
    低成本细胞计数器细胞计数的方法和算法

    公开(公告)号：US07764821B2

    公开(公告)日：2010-07-27

    申请号：US11434321

    申请日：2006-05-12

    申请人： Frank A. W. Coumans ,  Jan Greve ,  Frank P. Modica ,  Leon W. M. M. Terstappen ,  Arjan G. J. Tibbe ,  John A. Verrant

    发明人： Frank A. W. Coumans ,  Jan Greve ,  Frank P. Modica ,  Leon W. M. M. Terstappen ,  Arjan G. J. Tibbe ,  John A. Verrant

    IPC分类号： G06K9/00

    CPC分类号： C12M41/36 ,  G01N15/1475

    摘要： The enumeration of cells in fluids by flow cytometry is widely used across many disciplines such as assessment of leukocyte subsets in different bodily fluids or of bacterial contamination in environmental samples, food products and bodily fluids. For many applications the cost, size and complexity of the instruments prevents wider use, for example, CD4 analysis in HIV monitoring in resource-poor countries. The novel device, methods and algorithms disclosed herein largely overcome these limitations. Briefly, all cells in a biological sample are fluorescently labeled, but only the target cells are also magnetically labeled. In addition, non-magnetically labeled cells are imaged for viability in a modified slide configuration. The labeled sample, in a chamber or cuvet, is placed between two wedge-shaped magnets to selectively move the magnetically labeled cells to the observation surface of the cuvet. An LED illuminates the cells and a CCD camera captures the images of the fluorescent light emitted by the target cells. Image analysis performed with a novel algorithm provides a count of the cells on the surface that can be related to the target cell concentration of the original sample. The compact cytometer system provides a rugged, affordable and easy-to-use technique, which can be used in remote locations.

    摘要翻译： 通过流式细胞术对流体细胞的计数被广泛应用于许多学科，例如评估不同体液中的白细胞亚群或环境样品，食品和体液中的细菌污染。 对于许多应用，仪器的成本，尺寸和复杂性阻止了更广泛的使用，例如资源贫乏国家的艾滋病毒监测中的CD4分析。 本文公开的新颖的装置，方法和算法在很大程度上克服了这些限制。 简言之，生物样品中的所有细胞都被荧光标记，但只有靶细胞也被磁性标记。 另外，非磁性标记的细胞在修改的载玻片配置中的存活力被成像。 将标记的样品置于室或铜管中，放置在两个楔形磁体之间，以选择性地将磁性标记的细胞移动到铜管的观察表面。 LED照亮细胞，CCD相机捕获由靶细胞发射的荧光的图像。 用新算法进行的图像分析提供了与原始样品的靶细胞浓度相关的表面上的细胞计数。 紧凑型细胞仪系统提供坚固，经济实惠且易于使用的技术，可用于偏远地区。

    Methods and algorithms for cell enumeration in a low-cost cytometer
    3.
    发明授权
    Methods and algorithms for cell enumeration in a low-cost cytometer 有权
    低成本细胞计数器细胞计数的方法和算法

    公开(公告)号：US08189899B2

    公开(公告)日：2012-05-29

    申请号：US12707578

    申请日：2010-02-17

    申请人： Frank A. W. Coumans ,  Jan Greve ,  Frank P. Modica ,  Leon W. M. M. Terstappen ,  Arjan G. J. Tibbe ,  John A. Verrant

    发明人： Frank A. W. Coumans ,  Jan Greve ,  Frank P. Modica ,  Leon W. M. M. Terstappen ,  Arjan G. J. Tibbe ,  John A. Verrant

    IPC分类号： G06K9/00

    CPC分类号： G01N15/1475 ,  G01N33/5094 ,  G01N33/54333

    摘要： The enumeration of cells in fluids by flow cytometry is widely used across many disciplines such as assessment of leukocyte subsets in different bodily fluids or of bacterial contamination in environmental samples, food products and bodily fluids. For many applications the cost, size and complexity of the instruments prevents wider use, for example, CD4 analysis in HIV monitoring in resource-poor countries. The novel device, methods and algorithms disclosed herein largely overcome these limitations. Briefly, all cells in a biological sample are fluorescently labeled, but only the target cells are also magnetically labeled. In addition, non-magnetically labeled cells are imaged for viability in a modified slide configuration. The labeled sample, in a chamber or cuvet, is placed between two wedge-shaped magnets to selectively move the magnetically labeled cells to the observation surface of the cuvet. An LED illuminates the cells and a CCD camera captures the images of the fluorescent light emitted by the target cells. Image analysis performed with a novel algorithm provides a count of the cells on the surface that can be related to the target cell concentration of the original sample. The compact cytometer system provides a rugged, affordable and easy-to-use technique, which can be used in remote locations.

    摘要翻译： 通过流式细胞术对流体细胞的计数被广泛应用于许多学科，例如评估不同体液中的白细胞亚群或环境样品，食品和体液中的细菌污染。 对于许多应用，仪器的成本，尺寸和复杂性阻止了更广泛的使用，例如资源贫乏国家的艾滋病毒监测中的CD4分析。 本文公开的新颖的装置，方法和算法在很大程度上克服了这些限制。 简言之，生物样品中的所有细胞都被荧光标记，但只有靶细胞也被磁性标记。 另外，非磁性标记的细胞在修改的载玻片配置中的存活力被成像。 将标记的样品置于室或铜管中，放置在两个楔形磁体之间，以选择性地将磁性标记的细胞移动到铜管的观察表面。 LED照亮细胞，CCD相机捕获由靶细胞发射的荧光的图像。 用新算法进行的图像分析提供了与原始样品的靶细胞浓度相关的表面上的细胞计数。 紧凑型细胞仪系统提供坚固，经济实惠且易于使用的技术，可用于偏远地区。

    METHODS AND COMPOSITION TO GENERATE UNIQUE SEQUENCE DNA PROBES LABELING OF DNA PROBES AND THE USE OF THESE PROBES
    7.
    发明申请
    METHODS AND COMPOSITION TO GENERATE UNIQUE SEQUENCE DNA PROBES LABELING OF DNA PROBES AND THE USE OF THESE PROBES 有权
    生成DNA探针的独特序列DNA探针的方法和组合以及这些探针的使用

    公开(公告)号：US20090220955A1

    公开(公告)日：2009-09-03

    申请号：US12067532

    申请日：2006-09-20

    申请人： John A. Verrant

    发明人： John A. Verrant

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C40B30/04

    CPC分类号： C12Q1/6886 ,  C12Q1/34 ,  C12Q1/6811 ,  C12Q1/6876 ,  C12Q2600/106 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2533/101 ,  C12Q2531/113 ,  C12Q2521/301 ,  C12Q2563/131 ,  C12Q2563/103

    摘要： The invention relates generally to the field of identification of DNA sequences, genes or chromosomes. Methods and composition to obtain Unique Sequence DNA probes are provided. Composition comprises of any double stranded DNA containing Unique Sequences from which the repetitive sequences are eliminated according to the method described in this invention. The invention also relates to the preservation of cells that have been identified after immunuomagnetic selection and fluorescent labeling in order to further interrogate the cells of interest. Furthermore the invention relates to genetic analysis of cells that have been identified after immunomagnetic selection and fluorescent labeling.

    摘要翻译： 本发明一般涉及DNA序列，基因或染色体的鉴定领域。 提供了获得独特序列DNA探针的方法和组成。 组合物包含根据本发明描述的方法从其中排除重复序列的任何含有唯一序列的双链DNA。 本发明还涉及在免疫磁性选择和荧光标记之后鉴定的细胞的保存以进一步询问感兴趣的细胞。 此外，本发明涉及在免疫磁性选择和荧光标记之后鉴定的细胞的遗传分析。