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    Method for forming polymerized microfluidic devices
    3.
    发明授权
    Method for forming polymerized microfluidic devices 有权
    形成聚合微流体装置的方法

    公开(公告)号：US08394312B1

    公开(公告)日：2013-03-12

    申请号：US13237027

    申请日：2011-09-20

    申请人： Gregory J. Sommer ,  Anson V. Hatch ,  Ying-Chih Wang ,  Anup K. Singh ,  Ronald F. Renzi ,  Mark R. Claudnic

    发明人： Gregory J. Sommer ,  Anson V. Hatch ,  Ying-Chih Wang ,  Anup K. Singh ,  Ronald F. Renzi ,  Mark R. Claudnic

    IPC分类号： B81B7/00

    CPC分类号： B01L3/502707 ,  B81B2201/058 ,  B81C1/00119

    摘要： Methods for making a microfluidic device according to embodiments of the present invention include defining˜cavity. Polymer precursor solution is positioned in the cavity, and exposed to light to begin the polymerization process and define a microchannel. In some embodiments, after the polymerization process is partially complete, a solvent rinse is performed, or fresh polymer precursor introduced into the microchannel. This may promote removal of unpolymerized material from the microchannel and enable smaller feature sizes. The polymer precursor solution may contain an iniferter. Polymerized features therefore may be capped with the iniferter, which is photoactive. The iniferter may aid later binding of a polyacrylamide gel to the microchannel surface.

    摘要翻译： 根据本发明的实施例的制造微流体装置的方法包括限定腔。 将聚合物前体溶液定位在空腔中并暴露于光以开始聚合过程并限定微通道。 在一些实施方案中，在聚合过程部分完成之后，进行溶剂漂洗，或引入微通道的新鲜聚合物前体。 这可以促进从微通道去除未聚合的材料，并且能够实现更小的特征尺寸。 聚合物前体溶液可以含有引发剂。 因此，聚合特征可以被具有光活性的引发剂覆盖。 引发剂可以帮助以后将聚丙烯酰胺凝胶结合到微通道表面。

    Method for forming polymerized microfluidic devices
    4.
    发明授权
    Method for forming polymerized microfluidic devices 有权
    形成聚合微流体装置的方法

    公开(公告)号：US08047829B1

    公开(公告)日：2011-11-01

    申请号：US12321881

    申请日：2009-01-26

    申请人： Gregory J. Sommer ,  Anson V. Hatch ,  Ying-Chih Wang ,  Anup K. Singh ,  Ronald F. Renzi ,  Mark R. Claudnic

    发明人： Gregory J. Sommer ,  Anson V. Hatch ,  Ying-Chih Wang ,  Anup K. Singh ,  Ronald F. Renzi ,  Mark R. Claudnic

    IPC分类号： B81B7/00

    CPC分类号： B01L3/502707 ,  B81B2201/058 ,  B81C1/00119

    摘要： Methods for making a micofluidic device according to embodiments of the present invention include defining a cavity. Polymer precursor solution is positioned in the cavity, and exposed to light to begin the polymerization process and define a microchannel. In some embodiments, after the polymerization process is partially complete, a solvent rinse is performed, or fresh polymer precursor introduced into the microchannel. This may promote removal of unpolymerized material from the microchannel and enable smaller feature sizes. The polymer precursor solution may contain an iniferter. Polymerized features therefore may be capped with the iniferter, which is photoactive. The iniferter may aid later binding of a polyacrylamide gel to the microchannel surface.

    摘要翻译： 根据本发明的实施例的制造微流体装置的方法包括限定空腔。 将聚合物前体溶液定位在空腔中并暴露于光以开始聚合过程并限定微通道。 在一些实施方案中，在聚合过程部分完成之后，进行溶剂漂洗，或引入微通道的新鲜聚合物前体。 这可以促进从微通道去除未聚合的材料并且使得能够更小的特征尺寸。 聚合物前体溶液可以含有引发剂。 因此，聚合特征可以被具有光活性的引发剂覆盖。 引发剂可以帮助以后将聚丙烯酰胺凝胶结合到微通道表面。

    Microelectroporation device for genomic screening
    5.
    发明授权
    Microelectroporation device for genomic screening 有权
    用于基因组筛选的微电极装置

    公开(公告)号：US08828736B2

    公开(公告)日：2014-09-09

    申请号：US13173180

    申请日：2011-06-30

    申请人： Thomas D. Perroud ,  Ronald F. Renzi ,  Oscar Negrete ,  Mark R. Claudnic

    发明人： Thomas D. Perroud ,  Ronald F. Renzi ,  Oscar Negrete ,  Mark R. Claudnic

    IPC分类号： G01N25/08 ,  C12M1/42 ,  B01L3/00

    CPC分类号： B01L3/502761 ,  B01L2200/0668 ,  B01L2300/0645 ,  B01L2300/0864 ,  C12M35/02 ,  Y10T436/2575

    摘要： We have developed an microelectroporation device that combines microarrays of oligonucleotides, microfluidic channels, and electroporation for cell transfection and high-throughput screening applications (e.g. RNA interference screens). Microarrays allow the deposition of thousands of different oligonucleotides in microscopic spots. Microfluidic channels and microwells enable efficient loading of cells into the device and prevent cross-contamination between different oligonucleotides spots. Electroporation allows optimal transfection of nucleic acids into cells (especially hard-to-transfect cells such as primary cells) by minimizing cell death while maximizing transfection efficiency. This invention has the advantage of a higher throughput and lower cost, while preventing cross-contamination compared to conventional screening technologies. Moreover, this device does not require bulky robotic liquid handling equipment and is inherently safer given that it is a closed system.

    摘要翻译： 我们开发了一种微电极装置，其结合寡核苷酸微阵列，微流体通道和电穿孔用于细胞转染和高通量筛选应用（例如RNA干扰筛选）。 微阵列允许在微观斑点中沉积数千种不同的寡核苷酸。 微流体通道和微孔能够有效地将细胞装载到装置中并防止不同寡核苷酸斑点之间的交叉污染。 电穿孔允许通过最大限度地减少细胞死亡同时使转染效率最大化将核酸最佳地转染到细胞（特别是难转染细胞，如原代细胞）中。 本发明具有较高的生产能力和较低的成本，同时防止与常规筛选技术相比的交叉污染。 此外，该装置不需要庞大的机器人液体处理设备，并且由于它是封闭的系统，所以固有地更安全。

    MICROELECTROPORATION DEVICE FOR GENOMIC SCREENING
    7.
    发明申请
    MICROELECTROPORATION DEVICE FOR GENOMIC SCREENING 有权
    用于基因检测的微电子设备

    公开(公告)号：US20120004144A1

    公开(公告)日：2012-01-05

    申请号：US13173180

    申请日：2011-06-30

    申请人： Thomas D. Perroud ,  Ronald F. Renzi ,  Oscar Negrete ,  Mark R. Claudnic

    发明人： Thomas D. Perroud ,  Ronald F. Renzi ,  Oscar Negrete ,  Mark R. Claudnic

    IPC分类号： C40B60/12

    CPC分类号： B01L3/502761 ,  B01L2200/0668 ,  B01L2300/0645 ,  B01L2300/0864 ,  C12M35/02 ,  Y10T436/2575

    摘要： We have developed an microelectroporation device that combines microarrays of oligonucleotides, microfluidic channels, and electroporation for cell transfection and high-throughput screening applications (e.g. RNA interference screens). Microarrays allow the deposition of thousands of different oligonucleotides in microscopic spots. Microfluidic channels and microwells enable efficient loading of cells into the device and prevent cross-contamination between different oligonucleotides spots. Electroporation allows optimal transfection of nucleic acids into cells (especially hard-to-transfect cells such as primary cells) by minimizing cell death while maximizing transfection efficiency. This invention has the advantage of a higher throughput and lower cost, while preventing cross-contamination compared to conventional screening technologies. Moreover, this device does not require bulky robotic liquid handling equipment and is inherently safer given that it is a closed system.

    摘要翻译： 我们开发了一种微电极装置，其结合寡核苷酸微阵列，微流体通道和电穿孔用于细胞转染和高通量筛选应用（例如RNA干扰筛选）。 微阵列允许在微观斑点中沉积数千种不同的寡核苷酸。 微流体通道和微孔能够有效地将细胞装载到装置中并防止不同寡核苷酸斑点之间的交叉污染。 电穿孔允许通过最大限度地减少细胞死亡同时使转染效率最大化将核酸最佳地转染到细胞（特别是难转染细胞，如原代细胞）中。 本发明具有较高的生产能力和较低的成本，同时防止与常规筛选技术相比的交叉污染。 此外，该装置不需要庞大的机器人液体处理设备，并且由于它是封闭的系统，所以固有地更安全。

    ION CHAMBER BASED NEUTRON DETECTORS
    8.
    发明申请
    ION CHAMBER BASED NEUTRON DETECTORS 有权
    基于离子室的中子探测器

    公开(公告)号：US20130020492A1

    公开(公告)日：2013-01-24

    申请号：US13559370

    申请日：2012-07-26

    申请人： Mark S. Derzon ,  Paul C. Galambos ,  Ronald F. Renzi

    发明人： Mark S. Derzon ,  Paul C. Galambos ,  Ronald F. Renzi

    IPC分类号： G01T3/00

    CPC分类号： G01T3/008 ,  G01T3/00 ,  H01J47/02

    摘要： A neutron detector with monolithically integrated readout circuitry, including: a bonded semiconductor die; an ion chamber formed in the bonded semiconductor die; a first electrode and a second electrode formed in the ion chamber; a neutron absorbing material filling the ion chamber; and the readout circuitry which is electrically coupled to the first and second electrodes. The bonded semiconductor die includes an etched semiconductor substrate bonded to an active semiconductor substrate. The readout circuitry is formed in a portion of the active semiconductor substrate. The ion chamber has a substantially planar first surface on which the first electrode is formed and a substantially planar second surface, parallel to the first surface, on which the second electrode is formed. Desirably, the distance between the first electrode and the second electrode may be equal to or less than the 50% attenuation length for neutrons in the neutron absorbing material filling the ion chamber.

    摘要翻译： 一种具有单片集成读出电路的中子检测器，包括：键合半导体管芯; 形成在所述键合半导体管芯中的离子室; 形成在所述离子室中的第一电极和第二电极; 填充离子室的中子吸收材料; 以及电耦合到第一和第二电极的读出电路。 键合的半导体管芯包括结合到有源半导体衬底的蚀刻半导体衬底。 读出电路形成在有源半导体衬底的一部分中。 离子室具有基本上平面的第一表面，第一电极形成在其上，并且平行于第一表面的基本平坦的第二表面，在其上形成第二电极。 理想地，第一电极和第二电极之间的距离可以等于或小于填充离子室的中子吸收材料中的中子的50％的衰减长度。

    Modular optical detector system
    10.
    发明授权
    Modular optical detector system 有权
    模块化光学检测器系统

    公开(公告)号：US06998598B2

    公开(公告)日：2006-02-14

    申请号：US10633794

    申请日：2003-08-04

    申请人： Brent A. Horn ,  Ronald F. Renzi

    发明人： Brent A. Horn ,  Ronald F. Renzi

    IPC分类号： H01J3/14 ,  H01J40/14 ,  H01J5/16

    CPC分类号： G01N30/74 ,  B01L3/5027 ,  G01N21/645 ,  G01N2201/024

    摘要： A modular optical detector system. The detector system is designed to detect the presence of molecules or molecular species by inducing fluorescence with exciting radiation and detecting the emitted fluorescence. Because the system is capable of accurately detecting and measuring picomolar concentrations it is ideally suited for use with microchemical analysis systems generally and capillary chromatographic systems in particular. By employing a modular design, the detector system provides both the ability to replace various elements of the detector system without requiring extensive realignment or recalibration of the components as well as minimal user interaction with the system. In addition, the modular concept provides for the use and addition of a wide variety of components, including optical elements (lenses and filters), light sources, and detection means, to fit particular needs.

    摘要翻译： 模块化光学检测器系统。 探测器系统设计用于通过用激发辐射诱导荧光并检测发射的荧光来检测分子或分子物质的存在。 因为该系统能够精确地检测和测量皮摩尔浓度，所以它理想地适用于通常用于微量化学分析系统和特别是毛细管色谱系统。 通过采用模块化设计，检测器系统提供了替换检测器系统的各种元件的能力，而不需要对组件进行大量的重新对准或重新校准，以及最小化与系统的用户交互。 此外，模块化概念提供了各种组件的使用和添加，包括光学元件（透镜和滤光片），光源和检测装置，以适应特殊需要。