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公开(公告)号：US08895292B2
公开(公告)日：2014-11-25
申请号：US12421252
申请日：2009-04-09
申请人： Hans Söderlund , Ari Hokkanen , Kari Kataja , Ingmar Stuns , Kai Kolari , Heli Siren , Stella Rovio , Reetta Satokari , Jan Rautio
发明人： Hans Söderlund , Ari Hokkanen , Kari Kataja , Ingmar Stuns , Kai Kolari , Heli Siren , Stella Rovio , Reetta Satokari , Jan Rautio
CPC分类号： G01N33/54326 , B01L3/50273 , B01L3/502761 , B01L2200/027 , B01L2200/0652 , B01L2200/0668 , B01L2200/0684 , B01L2200/10 , B01L2300/0636 , B01L2300/0681 , B01L2300/087 , B01L2300/16 , B01L2400/086 , B01L2400/088 , G01N33/54366 , G01N35/0098
摘要： A microfluidic chip device (MCD) and its use for performing miniaturized assays on magnetic microbeads (MMs) are described. The MCD is particularly useful for carrying out miniaturized transcript analysis by aiding affinity capturing (TRAC) assays, including PCR. The MCD comprises at least one reaction chamber with sealable liquid connections and at least one fluidic pillar filter in each chamber. The fluidic pillar filter comprises rods with spacings allowing MMs to pass. The sealable liquid connections feed liquid to the reaction chamber, wherein air bubbles are removed. The liquid stream contacts the MMs, which are manipulated with a magnetic rod. The liquid connections enable trapping of the MMs behind the pillar filters or in the channel, while the liquid is changed.
摘要翻译： 描述了一种微流体芯片器件（MCD）及其在磁性微珠（MM）上进行微型化测定的用途。 MCD通过辅助亲和力捕获（TRAC）测定（包括PCR）来进行小型化的转录物分析特别有用。 MCD包括至少一个具有可密封液体连接的反应室和每个室中的至少一个流体柱过滤器。 流体柱过滤器包括具有允许MMs通过的间隔的杆。 可密封的液体连接件将液体供应到反应室，其中除去气泡。 液体流接触MM，它们用磁力棒操纵。 当液体改变时，液体连接可以将柱塞过滤器或通道中的MM捕获。
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2.发明授权Method and test kit for quantitative determination of polynucleotides in a mixture 失效用于定量测定混合物中多核苷酸的方法和测试试剂盒公开(公告)号：US07361461B2
公开(公告)日：2008-04-22
申请号：US10519403
申请日：2003-07-04
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6816 , C12Q2545/114 , C12Q2537/143 , C12Q2525/204 , C12Q2563/167
摘要： The invention relates to a method and test kit for quantitative determination of the amounts or relative proportions of polynucleotides in a mixture. The invention enables assessment of dynamic variations in a mixed population of organisms using affinity aided solution hybridization. The test kit comprises organized pools of polynucleotide probes having approximately the same number of nucleotides, which are distinguishable using resolution enabling tags providing the probes with different sizes. The resolution enabling tags may simultaneously act as tracer, affinity or primer tags. The probes are allowed to hybridize with affinity tagged analyte polynucleotides. The result is hybrids, recoverable on separation aiding tools provided with counterparts of the affinity tag. After the quantitative release of the probes, the individual probes can be amplified and recorded. The method and test kit are useful for determining hygienic and epidemiologic situations and evaluating the effect of antibiotic treatment and sanitary measures.
摘要翻译： 本发明涉及用于定量测定混合物中多核苷酸的量或相对比例的方法和测试试剂盒。 本发明能够使用亲和辅助溶液杂交来评估混合生物群体中的动态变化。 测试试剂盒包括具有大致相同数目的核苷酸的有组织的多核苷酸探针池，其可使用分辨率使得能够使提供不同尺寸的探针的标签是可区分的。 分辨率启用标签可以同时作为示踪剂，亲和力或引物标签。 使探针与亲和标记的分析物多核苷酸杂交。 结果是混合物，可在与亲和标签的对应物提供的分离辅助工具上恢复。 在探针定量释放后，可以扩增和记录各个探针。 该方法和检测试剂盒可用于确定卫生和流行病学情况，并评估抗生素治疗和卫生措施的效果。
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3.发明授权Method and test kit for quantitative determination of polynucleotides in a mixture 有权用于定量测定混合物中多核苷酸的方法和测试试剂盒公开(公告)号：US08143388B2
公开(公告)日：2012-03-27
申请号：US12105960
申请日：2008-04-18
CPC分类号： C12Q1/6816 , C12Q2545/114 , C12Q2537/143 , C12Q2525/204 , C12Q2563/167
摘要： The invention relates to a method and test kit for quantitative determination of the amounts or relative proportions of polynucleotides in a mixture. The invention enables assessment of dynamic variations in a mixed population of organisms using affinity aided solution hybridization. The test kit comprises organized pools of polynucleotide probes having approximately the same number of nucleotides, which are distinguishable using resolution enabling tags providing the probes with different sizes. The resolution enabling tags may simultaneously act as tracer, affinity or primer tags. The probes are allowed to hybridize with affinity tagged analyte polynucleotides. The result is hybrids, recoverable on separation aiding tools provided with counterparts of the affinity tag. After the quantitative release of the probes, the individual probes can be amplified and recorded. The method and test kit are useful for determining hygienic and epidemiologic situations and evaluating the effect of antibiotic treatment and sanitary measures.
摘要翻译： 本发明涉及用于定量测定混合物中多核苷酸的量或相对比例的方法和测试试剂盒。 本发明能够使用亲和辅助溶液杂交来评估混合生物群体中的动态变化。 测试试剂盒包括具有大致相同数目的核苷酸的有组织的多核苷酸探针池，其可使用分辨率使得能够使提供不同尺寸的探针的标签是可区分的。 分辨率启用标签可以同时作为示踪剂，亲和力或引物标签。 使探针与亲和标记的分析物多核苷酸杂交。 结果是混合物，可在与亲和标签的对应物提供的分离辅助工具上恢复。 在探针定量释放后，可以扩增和记录各个探针。 该方法和检测试剂盒可用于确定卫生和流行病学情况，并评估抗生素治疗和卫生措施的效果。
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4.发明授权Method for determining amounts of polynucleotide sequences present in cell or tissue samples 有权确定存在于细胞或组织样品中的多核苷酸序列的量的方法公开(公告)号：US09416401B2
公开(公告)日：2016-08-16
申请号：US10451640
申请日：2002-01-10
CPC分类号： C12Q1/6816 , C12Q2600/158 , C12Q2565/627 , C12Q2565/518 , C12Q2537/143
摘要： The invention is related to a method and test kits for quantitative determination of polynucleotide amounts present in a sample. The test kit comprises organized pools with polynucleotide probes having distinct sizes and optionally provided with tracer tags or primer tags. The probes are allowed to hybridize with affinity tagged analyte polynucleotides from the sample. The result is hybrids, which can be recovered on a separation aiding tool provided with the pair of the affinity tag. After the quantitative release of the probes, the probes are either directly recorded, or if primer tagged, they are amplified and optionally provided with a tracer tag before recording. The invention provides a sensitive and quantitative determination of the amount polynucleotides present in a cell or tissue sample and allows a quantitative assessment of variations in the amounts of polynucleotides as a response to inherent changes or due to external stimuli.
摘要翻译： 本发明涉及用于定量测定样品中存在的多核苷酸量的方法和测试试剂盒。 测试试剂盒包括具有不同大小的多核苷酸探针的有组织的池，并且任选地具有示踪剂标签或引物标签。 允许探针与来自样品的亲和标记的分析物多核苷酸杂交。 结果是混合物，其可以在具有一对亲和标签的分离辅助工具上回收。 探针的定量释放后，直接记录探针，或者如果引物标记，则它们被扩增，并且在记录之前可选地提供示踪剂标签。 本发明提供对存在于细胞或组织样品中的多核苷酸的量的敏感和定量的测定，并允许定量评估多核苷酸的量的变化，作为对固有变化或由于外部刺激的反应。
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公开(公告)号：US07091014B1
公开(公告)日：2006-08-15
申请号：US09423554
申请日：1999-03-11
申请人： Aristos Aristidou , John Londesborough , Merja Penttilä , Peter Richard , Laura Ruohonen , Hans Söderlund , Anita Teleman , Mervi Toivari
发明人： Aristos Aristidou , John Londesborough , Merja Penttilä , Peter Richard , Laura Ruohonen , Hans Söderlund , Anita Teleman , Mervi Toivari
CPC分类号： C12N9/0016 , C12N9/0006 , C12N9/1025 , C12N9/1205 , C12P7/06 , C12P7/18 , C12P7/625 , C12P13/04 , C12P13/08 , Y02E50/16 , Y02E50/17
摘要： The present invention relates to genetic engineering of production microorganisms used in biotechnology to improve their properties so that they produce useful products more efficiently. The microorganisms express at least one enzyme that causes the functional coupling of the oxidation and reduction of substrates by two pyridine nucleotide-linked dehydrogenase reactions with different specificities for the NAD/NADH and NADP/NADPH coenzyme couples and so facilitates the transfer of electrons between the two coenzyme couples through the said substrates. In particular the invention relates to increasing the yields of products such as ethanol or amino acids from carbon and nitrogen sources such as biomass comprising hexoes, pentoses or their polymers.
摘要翻译： 本发明涉及用于生物技术的生产微生物的基因工程，以改善其性质，使得它们更有效地生产有用的产品。 微生物表达至少一种酶，其通过与NAD / NADH和NADP / NADPH辅酶偶合的不同特异性的两个吡啶核苷酸连接的脱氢酶反应引起底物的氧化和还原的功能偶联，并且因此促进电子在 两种辅酶对通过所述底物。 特别地，本发明涉及从碳和氮源例如包含己糖，戊糖或其聚合物的生物质中提高产物如乙醇或氨基酸的产率。
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公开(公告)号：US20140044744A1
公开(公告)日：2014-02-13
申请号：US14112220
申请日：2012-04-18
申请人： Kristiina Takkinen , Marja-Leena Laukkanen , Hans Söderlund , Sirpa Jylhä , Heidi Holkeri , Merja Niemi , Juha Rouvinen
发明人： Kristiina Takkinen , Marja-Leena Laukkanen , Hans Söderlund , Sirpa Jylhä , Heidi Holkeri , Merja Niemi , Juha Rouvinen
IPC分类号： C07K14/415
CPC分类号： C07K14/415 , A61K39/36
摘要： The present invention provides mutant polypeptides useful as hypoallergens. More specifically the present invention provides mutant Bet v 1 proteins and the use of such polypeptides as hypoallergens for desensitizing against birch pollen allergies. Furthermore, the invention provides vaccine formulations comprising such polypeptides; the use of such formulations; and to methods of vaccination against birch pollen allergy.
摘要翻译： 本发明提供用作低过敏原的突变体多肽。 更具体地，本发明提供了突变体Bet v 1蛋白质，以及这些多肽作为低过敏原用于对桦树花粉过敏进行脱敏的用途。 此外，本发明提供了包含这种多肽的疫苗制剂; 使用这种制剂; 以及针对桦树花粉过敏的接种方法。
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7.发明申请公开(公告)号：US20100086552A1
公开(公告)日：2010-04-08
申请号：US12523151
申请日：2008-01-29
申请人： Sirpa Jylhä , Merja Niemi , Juha Rouvinen , Marja-Leena Laukkanen , Kristiina Takkinen , Hans Söderlund , Soili Mäkinen-Kiljunen , Tari Haahtela
发明人： Sirpa Jylhä , Merja Niemi , Juha Rouvinen , Marja-Leena Laukkanen , Kristiina Takkinen , Hans Söderlund , Soili Mäkinen-Kiljunen , Tari Haahtela
CPC分类号： G01N33/6854 , C07K16/18 , C07K2299/00 , G01N33/6878 , G01N2333/795 , G01N2500/02 , G01N2800/24
摘要： The present invention relates to human IgE antibodies and derivatives thereof, which bind non-continuous planar allergenic epitope, such as in β-lactoglobulin, with high affinity and specificity. The present invention also relates to processes for making and engineering such allergen binding monoclonal antibodies and to methods for using these antibodies and derivatives thereof in the field of immunodiagnostics and immunotherapy.
摘要翻译： 本发明涉及以高亲和力和特异性结合非连续平面变应原表位的人IgE抗体及其衍生物，例如在β-乳球蛋白中。 本发明还涉及制备和制备这种变应原结合单克隆抗体的方法以及在免疫诊断和免疫治疗领域中使用这些抗体及其衍生物的方法。
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公开(公告)号：US20100311604A1
公开(公告)日：2010-12-09
申请号：US12523140
申请日：2008-01-29
申请人： Kristiina Takkinen , Juha Rouvinen , Merja Niemi , Sirpa Jylhä , Marja-Leena Laukkanen , Hans Söderlund
发明人： Kristiina Takkinen , Juha Rouvinen , Merja Niemi , Sirpa Jylhä , Marja-Leena Laukkanen , Hans Söderlund
IPC分类号： C40B30/04
CPC分类号： C07K16/28 , C07K2317/622 , C40B30/04 , C40B40/10
摘要： This invention relates to protein engineering technology. More particularly, the present invention relates to a method for preparing human IgE antibodies and derivatives thereof, which bind epitope structures that are weakly IgG or IgM immunoreactive.
摘要翻译： 本发明涉及蛋白质工程技术。 更具体地说，本发明涉及一种制备人IgE抗体及其衍生物的方法，该方法结合了弱IgG或IgM免疫反应性的表位结构。
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公开(公告)号：US06344341B1
公开(公告)日：2002-02-05
申请号：US09446920
申请日：1999-12-30
申请人： Sirkka Keränen , Jaana Toikkanen , Ville Tieaho , Hans Söderlund
发明人： Sirkka Keränen , Jaana Toikkanen , Ville Tieaho , Hans Söderlund
IPC分类号： C12P2106
摘要： This invention relates to recombinant-DNA-technology. Specifically this invention relates to new recombinant yeast cells transformed with SEB1 gene. Yeast cells transformed with several copies of SEB1 gene, or overexpressing the Seb1 protein by some other means, have an increased capacity to produce secreted foreign or endogenous proteins. Further, said new recombinant cells, when transformed with genes expressing suitable hydrolytic enzymes can utilize appropriate macromolecular compounds more efficiently, which results in increased cell mass production and/or more versatile utilization of the compounds in relevant biotechnical applications.
摘要翻译： 本发明涉及重组DNA技术。 具体地说，本发明涉及用SEB1基因转化的新的重组酵母细胞。 用几个拷贝的SEB1基因转化的酵母细胞，或通过其他方法过表达Seb1蛋白，具有增加产生分泌的外源或内源蛋白质的能力。 此外，当用表达合适的水解酶的基因转化时，所述新的重组细胞可以更有效地利用合适的大分子化合物，这导致在相关生物技术应用中化合物增加的细胞大量产生和/或更通用的利用。
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公开(公告)号：US06235535B1
公开(公告)日：2001-05-22
申请号：US09202976
申请日：1998-12-24
IPC分类号： G01N2176
CPC分类号： G01N33/5436 , G01N33/533 , Y10S436/80 , Y10S436/805 , Y10S436/819 , Y10S436/829
摘要： This invention relates to a fluorescence-based immunoassay method for the detection of an analyte, or for the measurement of its concentration in a biological sample. The method is based on the ability of a multivalent analyte to induce aggregation of receptor molecules labeled with a fluorophore, which molecules are anchored to and are freely mobile on a lipid membrane, and thereby cause changes in the fluorescence.
摘要翻译： 本发明涉及一种用于检测分析物或用于测量其在生物样品中的浓度的基于荧光的免疫测定方法。 该方法基于多价分析物诱导用荧光团标记的受体分子聚集的能力，该分子被锚定在脂质膜上并且可自由移动，从而引起荧光的变化。
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