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1.发明授权Water soluble tri-substituted 1, 2-dioxetane compounds having increased storage stability, synthetic processes and intermediates 失效具有增加储存稳定性的水溶性三取代1,2-二氧环乙烷化合物，合成方法和中间体公开(公告)号：US06410751B1
公开(公告)日：2002-06-25
申请号：US09839472
申请日：2001-04-20
IPC分类号： C07F909
CPC分类号： G01N33/582 , C07C69/734 , C07C2603/74 , C07D321/00 , C07F9/12 , C07F9/65512 , G01N33/5308 , G01N33/54326
摘要： Stable, enzymatically triggered chemiluminescent 1,2-dioxetanes with improved water solubility and storage stability are provided as well as synthetic processes and intermediates used in their preparation. Dioxetanes further substituted with two or more water-solubilizing groups disposed on the dioxetane structure and an additional fluorine atom or lower alkyl group provide superior performance by eliminating the problem of reagent carryover when used in assays performed on capsule chemistry analytical systems. These dioxetanes display substantially improved stability on storage. Compositions comprising these dioxetanes, a non-polymeric cationic surfactant enhancer and optionally a fluorescer, for providing enhanced chemiluminescence are also provided.
摘要翻译： 提供了具有改善的水溶性和储存稳定性的稳定的，酶促触发的化学发光1,2-二氧杂环丁烷，以及用于其制备的合成方法和中间体。 二氧杂环丁烷进一步被设置在二氧杂环丁烷结构上的水溶性基团和另外的氟原子或低级烷基取代，当用于胶囊化学分析系统的测定中时，通过消除试剂残留的问题而提供优异的性能。 这些二氧杂环丁烷在储存时显示出显着提高的稳定性。 还提供了包含这些二氧杂环丁烷的组合物，非聚合阳离子表面活性剂增强剂和任选的荧光剂，用于提供增强的化学发光。
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2.发明授权Water soluble tri-substituted 1,2-dioxetane compounds having increased storage stability, synthetic processes and intermediates 失效具有增加储存稳定性，合成方法和中间体的水溶性三取代1,2-二氧杂环丁烷化合物
公开(公告)号：US6036892A
公开(公告)日：2000-03-14
申请号：US101331
申请日：1998-07-07
IPC分类号： G01N33/535 , C07C69/00 , C07D321/00 , C07F9/12 , C07F9/655 , C09K11/06 , C09K11/07 , G01N33/53 , G01N33/543 , G01N33/58 , C09K3/00 , C07C69/76 , C07F9/02 , C07F9/06 , C07H17/08
CPC分类号： C07C69/00 , C07D321/00 , C07F9/12 , C07F9/65512 , G01N33/5308 , G01N33/54326 , G01N33/582 , C07C2103/00
摘要： Stable, enzymatically triggered chemiluminescent 1,2-dioxetanes with improved water solubility and storage stability are provided as well as synthetic processes and intermediates used in their preparation. Dioxetanes further substituted with two or more water-solubilizing groups disposed on the dioxetane structure and an additional fluorine atom or lower alkyl group provide superior performance by eliminating the problem of reagent carryover when used in assays performed on capsule chemistry analytical systems. These dioxetanes display substantially improved stability on storage. Compositions comprising these dioxetanes, a non-polymeric cationic surfactant enhancer and optionally a fluorescer, for providing enhanced chemiluminescence are also provided.
摘要翻译： PCT No.PCT / US97 / 19618 Sec。 371日期：1998年7月7日 102（e）日期1998年7月7日PCT 1997年11月7日PCT公布。 第WO98 / 19989号公报 日期1998年5月14日提供稳定的酶促触发的化学发光1,2-二氧杂环丁烷，具有改善的水溶性和储存稳定性，以及用于其制备的合成方法和中间体。 二氧杂环丁烷进一步被设置在二氧杂环丁烷结构上的水溶性基团和另外的氟原子或低级烷基取代，当用于胶囊化学分析系统的分析中时，通过消除试剂残留的问题而提供优异的性能。 这些二氧杂环丁烷在储存时显示出显着提高的稳定性。 还提供了包含这些二氧杂环丁烷的组合物，非聚合阳离子表面活性剂增强剂和任选的荧光剂，用于提供增强的化学发光。
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公开(公告)号：US5721370A
公开(公告)日：1998-02-24
申请号：US748107
申请日：1996-11-08
IPC分类号： G01N33/535 , C07C69/00 , C07D305/08 , C07D321/00 , C07F9/12 , C07F9/655 , C09K11/06 , C09K11/07 , G01N21/76 , G01N33/53 , G01N33/543 , G01N33/58 , C07F9/28
CPC分类号： C07C69/734 , C07C67/343 , C07D321/00 , C07F9/12 , C07F9/65512 , G01N33/5308 , G01N33/54326 , G01N33/582 , C07C2103/74
摘要： Stable, enzymatically triggered chemiluminescent 1,2-dioxetanes with improved water solubility and storage stability are provided. Dioxetanes further substituted with two or more water-solubilizing groups disposed on the dioxetane structure and an additional fluorine atom or lower alkyl group provide superior performance by eliminating the problem of reagent carryover when used in assays performed on capsule chemistry analytical systems. These dioxetanes display substantially improved stability on storage. Compositions comprising these dioxetanes, a non-polymeric cationic surfactant enhancer and optionally a fluorescer, for providing enhanced chemiluminescence are also provided.
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公开(公告)号：US06891057B2
公开(公告)日：2005-05-10
申请号：US10754750
申请日：2004-01-09
IPC分类号： C07D219/00 , C07D219/02 , C07D221/08 , C07F7/18 , C07F9/113 , C07F9/64 , C07F9/6553 , C09K11/06 , G01N33/58 , C07F9/02
CPC分类号： C07F9/113 , C07D219/02 , C07D221/08 , C07F7/1804 , C07F9/64 , C07F9/655372 , C09K11/06 , G01N33/582
摘要： Novel compounds comprising a C—C double bond substituted at one carbon with two oxygen or sulfur atom-containing groups are disclosed. The compounds are useful in methods and compositions for generating chemiluminescence by reaction with a peroxidase enzyme and a peroxide. The chemiluminescence thus produced can be used as a detectable signal in assays for peroxidase enzymes and in assays employing enzyme-labeled specific binding pairs.
摘要翻译： 公开了包含在一个碳上被两个含氧或含硫原子的基团取代的C-C双键的新型化合物。 该化合物可用于通过与过氧化物酶和过氧化物反应产生化学发光的方法和组合物。 由此产生的化学发光可以用作过氧化物酶测定中的可检测信号，并且在使用酶标记的特异性结合对的测定中可以使用。
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5.发明授权
公开(公告)号：US6139782A
公开(公告)日：2000-10-31
申请号：US358004
申请日：1999-07-21
IPC分类号： G01N33/532 , C07D219/00 , C07F1/04 , C07F9/572 , C07F9/576 , C07F9/64 , C07F9/6541 , C09K3/00 , C09K11/07 , C12N11/02 , C12N15/09 , C12Q1/42 , G01N21/78 , G01N27/447 , G01N33/53 , G01N33/535
CPC分类号： C07F9/6541 , C07F9/5765 , C07F9/64 , C12Q1/42 , C12Q2334/00 , Y10S435/968
摘要： Novel heterocyclic compounds which generate chemiluminescence on reaction with a phosphatase enzyme are provided as well as a process for their preparation and intermediates useful therein. The compounds comprise a nitrogen, oxygen or sulfur-containing heterocyclic ring system bearing an exocyclic carbon-carbon double bond. The double bond is further substituted at the distal carbon with a phosphate group and an oxygen or sulfur atom-containing group.Novel compositions further comprising a cationic aromatic compound (CAC) in addition to the heterocyclic phosphate compound are provided. The addition of the CAC in the composition greatly increases the production of chemiluminescence and provides improved detection sensitivity. Compositions further comprising an anionic surfactant and a non-ionic surfactant provide additional improvements in detection sensitivity. The novel chemiluminescent compounds and compositions are useful in methods for producing light and in assays for phosphatase enzymes and enzyme inhibitors and in assays employing enzyme-labeled specific binding pairs.
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6.发明授权
公开(公告)号：US6090571A
公开(公告)日：2000-07-18
申请号：US358002
申请日：1999-07-21
IPC分类号： G01N33/532 , C07D219/00 , C07F1/04 , C07F9/572 , C07F9/576 , C07F9/64 , C07F9/6541 , C09K3/00 , C09K11/07 , C12N11/02 , C12N15/09 , C12Q1/42 , G01N21/78 , G01N27/447 , G01N33/53 , G01N33/535
CPC分类号： C07F9/6541 , C07F9/5765 , C07F9/64 , C12Q1/42 , C12Q2334/00 , Y10S435/968
摘要： Novel heterocyclic compounds which generate chemiluminescence on reaction with a phosphatase enzyme are provided as well as a process for their preparation and intermediates useful therein. The compounds comprise a nitrogen, oxygen or sulfur-containing heterocyclic ring system bearing an exocyclic carbon-carbon double bond. The double bond is further substituted at the distal carbon with a phosphate group and an oxygen or sulfur atom-containing group.Novel compositions further comprising a cationic aromatic compound (CAC) in addition to the heterocyclic phosphate compound are provided. The addition of the CAC in the composition greatly increases the production of chemiluminescence and provides improved detection sensitivity. Compositions further comprising an anionic surfactant and a non-ionic surfactant provide additional improvements in detection sensitivity. The novel chemiluminescent compounds and compositions are useful in methods for producing light and in assays for phosphatase enzymes and enzyme inhibitors and in assays employing enzyme-labeled specific binding pairs.
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7.发明授权Aryl N-alkylacridanthiocarboxylate derivatives useful for chemiluminescent detection 失效可用于化学发光检测的N-烷基酰基羧酸芳基酯衍生物
公开(公告)号：US5523212A
公开(公告)日：1996-06-04
申请号：US228290
申请日：1994-04-15
IPC分类号： G01N21/78 , C07D219/02 , C07D219/04 , C07D219/06 , C09B15/00 , C09K11/07 , C12Q1/28 , C12Q1/68 , G01N21/76 , G01N33/532 , G01N33/535 , G01N33/566 , C07H5/04 , C12Q1/00 , C12Q1/26
CPC分类号： C07D219/06 , C07D219/02 , C07D219/04 , C12Q1/28 , Y10S435/81 , Y10S435/968
摘要： Aryl N-alkylacridanthiocarboxylate compounds which produce chemiluminescence. The compounds produce light with peroxide and peroxidase. The compounds are used as a substrate in assays for various analytes.
摘要翻译： 产生化学发光的N-烷基酰基羧酸芳酯化合物。 该化合物用过氧化物和过氧化物酶产生光。 该化合物用作各种分析物测定中的底物。
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公开(公告)号：US06696569B2
公开(公告)日：2004-02-24
申请号：US09833050
申请日：2001-04-11
IPC分类号： C07D21900
CPC分类号： C07F9/113 , C07D219/02 , C07D221/08 , C07F7/1804 , C07F9/64 , C07F9/655372 , C09K11/06 , G01N33/582
摘要： Novel compounds comprising a C—C double bond substituted at one carbon with two oxygen or sulfur atom-containing groups are disclosed. The compounds are useful in methods and compositions for generating chemiluminescence by reaction with a peroxidase enzyme and a peroxide. The chemiluminescence thus produced can be used as a detectable signal in assays for peroxidase enzymes and in assays employing enzyme-labeled specific binding pairs.
摘要翻译： 公开了包含在一个碳上被两个含氧或含硫原子的基团取代的C-C双键的新型化合物。 该化合物可用于通过与过氧化物酶和过氧化物反应产生化学发光的方法和组合物。 由此产生的化学发光可以用作过氧化物酶测定中的可检测信号，并且在使用酶标记的特异性结合对的测定中可以使用。
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9.发明授权Water soluble tri-substituted 1,2-dioxetane compounds having increased storage stability, synthetic processes and intermediates 失效具有增加储存稳定性，合成方法和中间体的水溶性三取代1,2-二氧杂环丁烷化合物公开(公告)号：US06353129B1
公开(公告)日：2002-03-05
申请号：US09839622
申请日：2001-04-20
IPC分类号： C07C6973
CPC分类号： G01N33/582 , C07C69/734 , C07C2603/74 , C07D321/00 , C07F9/12 , C07F9/65512 , G01N33/5308 , G01N33/54326
摘要： Stable, enzymatically triggered chemiluminescent 1,2-dioxetanes with improved water solubility and storage stability are provided as well as synthetic processes and intermediates used in their preparation. Dioxetanes further substituted with two or more water-solubilizing groups disposed on the dioxetane structure and an additional fluorine atom or lower alkyl group provide superior performance by eliminating the problem of reagent carryover when used in assays performed on capsule chemistry analytical systems. These dioxetanes display substantially improved stability on storage. Compositions comprising these dioxetanes, a non-polymeric cationic surfactant enhancer and optionally a fluorescer, for providing enhanced chemiluminescence are also provided.
摘要翻译： 提供了具有改善的水溶性和储存稳定性的稳定的，酶促触发的化学发光1,2-二氧杂环丁烷，以及用于其制备的合成方法和中间体。 二氧杂环丁烷进一步被设置在二氧杂环丁烷结构上的水溶性基团和另外的氟原子或低级烷基取代，当用于胶囊化学分析系统的测定中时，通过消除试剂残留的问题而提供优异的性能。 这些二氧杂环丁烷在储存时显示出显着提高的稳定性。 还提供了包含这些二氧杂环丁烷的组合物，非聚合阳离子表面活性剂增强剂和任选的荧光剂，用于提供增强的化学发光。
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10.发明授权Compounds, compositions and methods for generating chemiluminescence with phosphatase enzymes 失效用磷酸酶生成化学发光的化合物，组合物和方法公开(公告)号：US06296787B1
公开(公告)日：2001-10-02
申请号：US09557726
申请日：2000-04-26
IPC分类号： C09K300
CPC分类号： C07F9/6541 , C07F9/5765 , C07F9/64 , C12Q1/42 , C12Q2334/00 , Y10S435/968
摘要： Novel heterocyclic compounds which generate chemiluminescence on reaction with a phosphatase enzyme are provided as well as a process for their preparation and intermediates useful therein. The compounds comprise a nitrogen, oxygen or sulfur-containing heterocyclic ring system bearing an exocyclic carbon—carbon double bond. The double bond is further substituted at the distal carbon with a phosphate group and an oxygen or sulfur atom-containing group. Novel compositions further comprising a cationic aromatic compound (CAC) in addition to the heterocyclic phosphate compound are provided. The addition of the CAC in the composition greatly increases the production of chemiluminescence and provides improved detection sensitivity. Compositions further comprising an anionic surfactant and a non-ionic surfactant provide additional improvements in detection sensitivity. The novel chemiluminescent compounds and compositions are useful in methods for producing light and in assays for phosphatase enzymes and enzyme inhibitors and in assays employing enzyme-labeled specific binding pairs.
摘要翻译： 提供了与磷酸酶反应产生化学发光的新型杂环化合物以及其制备方法和可用于其中的中间体。 该化合物包含带有环外碳 - 碳双键的氮，氧或含硫杂环体系。 提供了双键在磷酸基和含氧或含硫原子的基团的末端碳上进一步取代的。还提供除了杂环磷酸酯化合物之外还包含阳离子芳族化合物（CAC）的新型组合物。 在组合物中添加CAC大大增加了化学发光的产生并提供了改善的检测灵敏度。 进一步包含阴离子表面活性剂和非离子表面活性剂的组合物提供检测灵敏度的另外的改进。 新颖的化学发光化合物和组合物可用于生产光的方法和用于磷酸酶和酶抑制剂的测定和使用酶标记的特异性结合对的测定中。
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