公开(公告)号：US5006055A

公开(公告)日：1991-04-09

申请号：US389860

申请日：1989-08-04

申请人： Helmut Lebisch ,  Rainer Basel ,  Georg Fuchs ,  Hermann Dietmar ,  Ernst Wipfelder ,  Wilhelm Hekele

发明人： Helmut Lebisch ,  Rainer Basel ,  Georg Fuchs ,  Hermann Dietmar ,  Ernst Wipfelder ,  Wilhelm Hekele

IPC分类号： H04R1/10 ,  B29C33/40 ,  B29C39/02 ,  H04R25/00 ,  H04R25/02

CPC分类号： B29C33/405 ,  B29C39/02 ,  H04R25/656 ,  H04R25/658 ,  B29L2031/7532 ,  H04R2225/025 ,  Y10S264/30

摘要： An apparatus for manufacturing an otoplastic. An otoplastic is manufactured directly in the ear of a hearing-impaired person in that a deformable envelope is pulled over a die or over a shell or over an overlayed over-shell. This apparatus is then supplied with flowing otoplastic material between the die and the envelope, being supplied therewith in the ear. The envelope expands and assumes the shape of the auditory canal. After hardening or curing of the otoplastic material in the ear of the hearing-impaired person, the envelope and the die are removed and a module of either an in-the-ear or of a behind-the-ear hearing aid is integrated therewith.

公开(公告)号：US4871502A

公开(公告)日：1989-10-03

申请号：US185794

申请日：1988-04-22

申请人： Helmut LeBisch ,  Rainer Basel ,  Georg Fuchs ,  Hermann Dietmar ,  Ernst Wipfelder ,  Wilhelm Hekele

发明人： Helmut LeBisch ,  Rainer Basel ,  Georg Fuchs ,  Hermann Dietmar ,  Ernst Wipfelder ,  Wilhelm Hekele

IPC分类号： H04R1/10 ,  B29C33/40 ,  B29C39/02 ,  H04R25/00 ,  H04R25/02

CPC分类号： B29C33/405 ,  B29C39/02 ,  H04R25/656 ,  H04R25/658 ,  B29L2031/7532 ,  H04R2225/025 ,  Y10S264/30

摘要： A method and apparatus for manufacturing an otoplastic. An otoplastic is manufactured directly in the ear of a hearing-impaired person in that a deformable envelope is pulled over a die or over a shell or over an overlayed over-shell. This apparatus is then supplied with flowing otoplastic material between the die and the envelope, being supplied therewith in the ear. The envelope expands and assumes the shape of the auditory canal. After hardening or curing of the otoplastic material in the ear of the hearing-impaired person, the envelope and the die are removed and a module of either an in-the-ear or of a behind-the-ear hearing aid is integrated therewith.

摘要翻译： 用于制造耳用塑料的方法和装置。 直立在听力障碍者的耳朵内制造耳用塑料，因为可变形的外壳被拉过模具或者在外壳上或覆盖在外壳上。 然后，该设备在模具和信封之间提供流动的塑料材料，并在其中提供。 信封膨胀并呈现出耳道的形状。 在听觉受损人员的耳朵中的耳用塑料材料硬化或固化之后，将信封和模具移除，将耳内或耳后助听器的模块整合在一起。

公开(公告)号：US20100190224A1

公开(公告)日：2010-07-29

申请号：US12593090

申请日：2008-03-27

申请人： Markus Poetter ,  Achim Marx ,  Georg Fuchs ,  Birgit Alber ,  Tobias Juergen Erb

发明人： Markus Poetter ,  Achim Marx ,  Georg Fuchs ,  Birgit Alber ,  Tobias Juergen Erb

IPC分类号： C12P7/42 ,  C12N15/74 ,  C12N9/00 ,  C12N1/21 ,  C12N15/63 ,  C07H21/04

CPC分类号： C12P7/52 ,  C12N9/506 ,  C12P7/42

摘要： The present invention relates to an isolated DNA, which is selected from the following sequences: a) a sequence according to SEQ ID No. 01, b) an intron-free sequence that is derived from a sequence according to a) and encodes the same protein or peptide as the sequence according to SEQ ID No. 01, c) a sequence that encodes a protein or peptide, which comprises the amino acid sequence according to SEQ ID No. 02, d) a sequence that is at least 80% identical to a sequence according to a) to c), e) a sequence that hybridizes with the antisense strand of a sequence according to one of the groups a) to d) or would hybridize taking into account degeneration of the genetic code, f) a derivative of a sequence according to one of the groups a) to e) obtained by substitution, addition, inversion and/or deletion of one or more bases, g) a sequence that corresponds to SEQ ID No. 01 within the degeneration of the genetic code, h) a sequence with neutral sense mutations of SEQ ID No. 01, and i) a sequence complementary to a sequence according to one of the groups a) to h). The invention further relates to a vector, the use of this vector for transformation of a cell, a transformed cell, a polypeptide, cells genetically engineered relative to their wild type, a method of production of a genetically engineered cell, the genetically engineered cell obtainable by this method, the use of this cell and a method of production of 3-hydroxyisobutyric acid or of a derivative thereof.

摘要翻译： 本发明涉及分离的DNA，其选自以下序列：a）根据SEQ ID No 01的序列，b）源自根据a）的序列的内含子序列，并编码它们 蛋白质或肽，其为编码蛋白质或肽的序列，其包含根据SEQ ID No.22的氨基酸序列，d）至少80％相同的序列 根据a）至c）的序列，e）与根据a）至d）中的一个组的序列的反义链杂交的序列或将考虑到遗传密码的变性而杂交，f）a 根据通过一个或多个碱基的取代，添加，倒位和/或缺失得到的a）至e）中的一个序列的序列的衍生物，g）在遗传变性中对应于SEQ ID No 01的序列 代码，h）具有SEQ ID No 01的中性有义突变的序列，和i） 与根据a）至h）中的一个组合的序列互补的序列。 本发明进一步涉及载体，该载体用于转化细胞，转化细胞，多肽，相对于其野生型基因工程改造的细胞，遗传工程细胞的生产方法，可获得的遗传工程改造的细胞 通过该方法，可以使用该细胞和3-羟基异丁酸或其衍生物的制造方法。

公开(公告)号：US20150218601A1

公开(公告)日：2015-08-06

申请号：US14624713

申请日：2015-02-18

申请人： Achim MARX ,  Markus Poetter ,  Stefan Buchholz ,  Alexander May ,  Hermann Siegert ,  Georg Fuchs ,  Birgit Alber ,  Lothar Eggeling

发明人： Achim MARX ,  Markus Poetter ,  Stefan Buchholz ,  Alexander May ,  Hermann Siegert ,  Georg Fuchs ,  Birgit Alber ,  Lothar Eggeling

IPC分类号： C12P7/42 ,  C12N9/88 ,  C08F120/10 ,  C12N9/10 ,  C08F120/06 ,  C12N9/16 ,  C12N9/02

CPC分类号： C12P7/42 ,  C08F120/06 ,  C08F120/10 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/1096 ,  C12N9/16 ,  C12N9/88 ,  C12P7/40 ,  C12P7/52 ,  C12P7/625 ,  C12Y102/04004 ,  C12Y103/99012 ,  C12Y206/01042 ,  C12Y301/02004 ,  C12Y402/01017

摘要： The present invention relates to a cell which has been modified in comparison with its wild type in such a way that it is capable of forming more, by comparison with its wild, 3-hydroxyisobutyric acid or polyhydroxyalkanoates based on 3-hydroxyisobutyric acid via methylmalonate-semialdehyde or 3-hydroxybutyryl-coenzyme A as precursors. The invention also relates to a method of generating a genetically modified cell, to the genetically modified cell obtainable by these methods, to a method of producing 3-hydroxyisobutyric acid or polyhydroxyalkanoates based on 3-hydroxyisobutyric acid, to a method of producing methacrylic acid or methacrylic esters, and to a method of producing polymethacrylic acid or polymethacrylic esters. The present invention furthermore relates to an isolated DNA, to a vector, to the use of this vector for transforming a cell, to a transformed cell, and to a polypeptide.

摘要翻译： 本发明涉及与其野生型相比已被修饰的细胞，使得其能够通过与其野生型3-羟基异丁酸或基于3-羟基异丁酸的聚羟基链烷酸通过甲基丙二酸酯 - 半醛或3-羟基丁酰辅酶A作为前体。 本发明还涉及生产基于3-羟基异丁酸的3-羟基异丁酸或聚羟基链烷酸酯的生产方法，该方法可以通过这些方法产生遗传修饰的细胞，生产甲基丙烯酸或其制备方法 甲基丙烯酸酯，以及生产聚甲基丙烯酸或聚甲基丙烯酸酯的方法。 本发明还涉及分离的DNA，载体，使用该载体转化细胞，转化细胞和多肽。

公开(公告)号：US09234218B2

公开(公告)日：2016-01-12

申请号：US12602593

申请日：2008-05-30

申请人： Achim Marx ,  Markus Poetter ,  Stefan Buchholz ,  Alexander May ,  Hermann Siegert ,  Birgit Alber ,  Georg Fuchs ,  Lothar Eggeling

发明人： Achim Marx ,  Markus Poetter ,  Stefan Buchholz ,  Alexander May ,  Hermann Siegert ,  Birgit Alber ,  Georg Fuchs ,  Lothar Eggeling

IPC分类号： C12P7/42 ,  C12P7/52 ,  C12N9/02 ,  C12P7/40 ,  C12P7/62 ,  C08F120/06 ,  C08F120/10 ,  C12N9/10 ,  C12N9/16 ,  C12N9/88

CPC分类号： C12P7/42 ,  C08F120/06 ,  C08F120/10 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/1096 ,  C12N9/16 ,  C12N9/88 ,  C12P7/40 ,  C12P7/52 ,  C12P7/625 ,  C12Y102/04004 ,  C12Y103/99012 ,  C12Y206/01042 ,  C12Y301/02004 ,  C12Y402/01017

摘要： The present invention relates to a process for the preparation of methacrylic acid or methacrylic esters, comprising the process steps of IA) preparation of 3-hydroxyisobutyric acid by a process comprising the process step of bringing a cell which has been genetically modified in comparison with its wild type in such a way that it is capable of forming more 3-hydroxyisobutyric acid, or polyhydroxyalkanoates based on 3-hydroxyisobutyric acid in comparison with its wild type, into contact with a nutrient medium comprising, as carbon source, carbohydrates, glycerol, carbon dioxide, methanol, L-valine or L-glutamate under conditions under which 3-hydroxyisobutyric acid or polyhydroxyalkanoates based on 3-hydroxyisobutyric acid are formed from the carbon source, if appropriate, isolation of the 3-hydroxyisobutyric acid from the nutrient medium and also, if appropriate, neutralization of the 3-hydroxyisobutyric acid, IB) dehydration of the 3-hydroxyisobutyric acid with formation of methacrylic acid and also, where appropriate, esterification methacrylic acid. The invention also relates to a process for the preparation of polymethacrylic acid or polymethacrylic esters.

摘要翻译： 本发明涉及一种制备甲基丙烯酸或甲基丙烯酸酯的方法，其包括以下工艺步骤：IA）通过包括以下步骤的方法制备3-羟基异丁酸，所述方法包括将经遗传修饰的细胞与其 野生型，使得其能够形成更多的3-羟基异丁酸或与其野生型相比基于3-羟基异丁酸的聚羟基链烷酸酯与营养培养基接触，所述营养培养基包含作为碳源的碳水化合物，甘油，碳 二氧化物，甲醇，L-缬氨酸或L-谷氨酸盐，其中3-羟基异丁酸或基于3-羟基异丁酸的聚羟基链烷酸酯由碳源形成的条件下，如果合适，则从营养培养基中分离出3-羟基异丁酸， 如果适当，中和3-羟基异丁酸，IB）3-羟基异丁酸脱水形成甲基丙烯酸 酸，并且在适当的情况下酯化甲基丙烯酸。 本发明还涉及一种制备聚甲基丙烯酸或聚甲基丙烯酸酯的方法。

公开(公告)号：US20130007741A1

公开(公告)日：2013-01-03

申请号：US13515022

申请日：2010-12-10

申请人： Matthias Britsch ,  Georg Fuchs ,  Colin Hirsch ,  Andreas Florath

发明人： Matthias Britsch ,  Georg Fuchs ,  Colin Hirsch ,  Andreas Florath

IPC分类号： G06F9/455

CPC分类号： G06F9/4401 ,  G06F9/45558 ,  G06F11/203 ,  G06F11/2038 ,  G06F2009/45575 ,  G06F2009/45591 ,  G06F2009/45595 ,  H04L67/10 ,  H04L69/40

摘要： A computer cluster includes: a first compartment, further including a plurality of first physical nodes configured to provide first resources for a first infrastructural virtual instance and second resources for guest virtual instances; and a second compartment, further including a plurality of second physical nodes configured to provide third resources for a second infrastructural virtual instance and fourth resources for guest virtual instances. Each of the plurality of physical nodes further includes a mass storage device, the mass storage device further including: a partition provided for booting the physical node; a partition as part of resources assigned to an infrastructural virtual instance; and a partition as part of other resources. The first infrastructural virtual instance and the second infrastructural virtual instance are configured for monitoring each other.

摘要翻译： 计算机集群包括：第一隔间，还包括多个第一物理节点，所述多个第一物理节点被配置为为第一基础设施虚拟实例提供第一资源，并为所述虚拟实例提供第二资源; 以及第二隔间，还包括多个第二物理节点，所述多个第二物理节点被配置为为第二基础设施虚拟实例提供第三资源，以及为访客虚拟实例提供第四资源。 所述多个物理节点中的每一个还包括大容量存储设备，所述大容量存储设备还包括：提供用于引导所述物理节点的分区; 作为分配给基础设施虚拟实例的资源的一部分的分区; 和一个分区作为其他资源的一部分。 第一个基础设施虚拟实例和第二个基础设施虚拟实例被配置为相互监视。

公开(公告)号：US08843624B2

公开(公告)日：2014-09-23

申请号：US13515035

申请日：2010-12-10

申请人： Matthias Britsch ,  Georg Fuchs ,  Colin Hirsch ,  Andreas Florath

发明人： Matthias Britsch ,  Georg Fuchs ,  Colin Hirsch ,  Andreas Florath

IPC分类号： G06F15/173 ,  G06F9/455 ,  G06F9/44 ,  G06F11/20 ,  H04L29/08 ,  H04L29/14

CPC分类号： G06F9/4401 ,  G06F9/45558 ,  G06F11/203 ,  G06F11/2038 ,  G06F2009/45575 ,  G06F2009/45591 ,  G06F2009/45595 ,  H04L67/10 ,  H04L69/40

摘要： A computer cluster includes: a first compartment, further including a plurality of first physical nodes configured to provide first resources for guest virtual instances, each of the plurality of first physical nodes further including a mass storage device, the mass storage device further including: a first partition provided for booting the first physical node; and a second partition as part of the first resources; and a second compartment, further including a plurality of second physical nodes configured to provide second resources for guest virtual instances, each of the plurality of second physical nodes further including a mass storage device, the mass storage device including: a third partition provided for booting the second physical node; and a fourth partition as part of the second resources. The first and the second physical nodes are further configured to provide third resources for a plurality of storage management processes.

摘要翻译： 计算机集群包括：第一隔间，还包括多个第一物理节点，被配置为为客户虚拟实例提供第一资源，所述多个第一物理节点中的每一个还包括大容量存储设备，所述大容量存储设备还包括： 提供用于引导第一物理节点的第一分区; 和第二分区作为第一资源的一部分; 以及第二隔间，还包括被配置为为访客虚拟实例提供第二资源的多个第二物理节点，所述多个第二物理节点中的每一个还包括大容量存储设备，所述大容量存储设备包括：第三分区，其用于引导 第二个物理节点; 以及作为第二资源的一部分的第四分区。 第一和第二物理节点被进一步配置为为多个存储管理过程提供第三资源。

公开(公告)号：US20100291644A1

公开(公告)日：2010-11-18

申请号：US12602593

申请日：2008-05-30

申请人： Achim Marx ,  Markus Poetter ,  Stefan Buchholz ,  Alexander May ,  Hermann Siegert ,  Birgit Alber ,  Georg Fuchs ,  Lothar Eggeling

发明人： Achim Marx ,  Markus Poetter ,  Stefan Buchholz ,  Alexander May ,  Hermann Siegert ,  Birgit Alber ,  Georg Fuchs ,  Lothar Eggeling

IPC分类号： C12P7/62 ,  C12P7/40 ,  C12P7/44

CPC分类号： C12P7/42 ,  C08F120/06 ,  C08F120/10 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/1096 ,  C12N9/16 ,  C12N9/88 ,  C12P7/40 ,  C12P7/52 ,  C12P7/625 ,  C12Y102/04004 ,  C12Y103/99012 ,  C12Y206/01042 ,  C12Y301/02004 ,  C12Y402/01017

摘要： The present invention relates to a process for the preparation of methacrylic acid or methacrylic esters, comprising the process steps of IA) preparation of 3-hydroxyisobutyric acid by a process comprising the process step of bringing a cell which has been genetically modified in comparison with its wild type in such a way that it is capable of forming more 3-hydroxyisobutyric acid, or polyhydroxyalkanoates based on 3-hydroxyisobutyric acid in comparison with its wild type, into contact with a nutrient medium comprising, as carbon source, carbohydrates, glycerol, carbon dioxide, methanol, L-valine or L-glutamate under conditions under which 3-hydroxyisobutyric acid or polyhydroxyalkanoates based on 3-hydroxyisobutyric acid are formed from the carbon source, if appropriate, isolation of the 3-hydroxyisobutyric acid from the nutrient medium and also, if appropriate, neutralization of the 3-hydroxyisobutyric acid, IB) dehydration of the 3-hydroxyisobutyric acid with formation of methacrylic acid and also, where appropriate, esterification methacrylic acid. The invention also relates to a process for the preparation of polymethacrylic acid or polymethacrylic esters.

摘要翻译： 本发明涉及一种制备甲基丙烯酸或甲基丙烯酸酯的方法，其包括以下工艺步骤：IA）通过包括以下步骤的方法制备3-羟基异丁酸，所述方法包括将经遗传修饰的细胞与其 野生型，使得其能够形成更多的3-羟基异丁酸或与其野生型相比基于3-羟基异丁酸的聚羟基链烷酸酯与营养培养基接触，所述营养培养基包含作为碳源的碳水化合物，甘油，碳 二氧化物，甲醇，L-缬氨酸或L-谷氨酸盐，其中3-羟基异丁酸或基于3-羟基异丁酸的聚羟基链烷酸酯由碳源形成的条件下，如果合适，则从营养培养基中分离出3-羟基异丁酸， 如果适当，中和3-羟基异丁酸，IB）3-羟基异丁酸脱水形成甲基丙烯酸 酸，并且在适当的情况下酯化甲基丙烯酸。 本发明还涉及一种制备聚甲基丙烯酸或聚甲基丙烯酸酯的方法。

公开(公告)号：US09135018B2

公开(公告)日：2015-09-15

申请号：US13515022

申请日：2010-12-10

申请人： Matthias Britsch ,  Georg Fuchs ,  Colin Hirsch ,  Andreas Florath ,  Ralf Hager

发明人： Matthias Britsch ,  Georg Fuchs ,  Colin Hirsch ,  Andreas Florath ,  Ralf Hager

IPC分类号： G06F9/455 ,  G06F9/44 ,  G06F11/20 ,  H04L29/08 ,  H04L29/14

CPC分类号： G06F9/4401 ,  G06F9/45558 ,  G06F11/203 ,  G06F11/2038 ,  G06F2009/45575 ,  G06F2009/45591 ,  G06F2009/45595 ,  H04L67/10 ,  H04L69/40

摘要： A computer cluster includes: a first compartment, further including a plurality of first physical nodes configured to provide first resources for a first infrastructural virtual instance and second resources for guest virtual instances; and a second compartment, further including a plurality of second physical nodes configured to provide third resources for a second infrastructural virtual instance and fourth resources for guest virtual instances. Each of the plurality of physical nodes further includes a mass storage device, the mass storage device further including: a partition provided for booting the physical node; a partition as part of resources assigned to an infrastructural virtual instance; and a partition as part of other resources. The first infrastructural virtual instance and the second infrastructural virtual instance are configured for monitoring each other.

摘要翻译： 计算机集群包括：第一隔间，还包括多个第一物理节点，所述多个第一物理节点被配置为为第一基础设施虚拟实例提供第一资源，以及为客体虚拟实例提供第二资源; 以及第二隔间，还包括多个第二物理节点，所述多个第二物理节点被配置为为第二基础设施虚拟实例提供第三资源，以及为访客虚拟实例提供第四资源。 所述多个物理节点中的每一个还包括大容量存储设备，所述大容量存储设备还包括：提供用于引导所述物理节点的分区; 作为分配给基础设施虚拟实例的资源的一部分的分区; 和一个分区作为其他资源的一部分。 第一个基础设施虚拟实例和第二个基础设施虚拟实例被配置为相互监视。

公开(公告)号：US20130013766A1

公开(公告)日：2013-01-10

申请号：US13515035

申请日：2010-12-10

申请人： Matthias Britsch ,  Georg Fuchs ,  Colin Hirsch ,  Andreas Florath

发明人： Matthias Britsch ,  Georg Fuchs ,  Colin Hirsch ,  Andreas Florath

IPC分类号： G06F15/173 ,  G06F11/00

CPC分类号： G06F9/4401 ,  G06F9/45558 ,  G06F11/203 ,  G06F11/2038 ,  G06F2009/45575 ,  G06F2009/45591 ,  G06F2009/45595 ,  H04L67/10 ,  H04L69/40

摘要： A computer cluster includes: a first compartment, further including a plurality of first physical nodes configured to provide first resources for guest virtual instances, each of the plurality of first physical nodes further including a mass storage device, the mass storage device further including: a first partition provided for booting the first physical node; and a second partition as part of the first resources; and a second compartment, further including a plurality of second physical nodes configured to provide second resources for guest virtual instances, each of the plurality of second physical nodes further including a mass storage device, the mass storage device including: a third partition provided for booting the second physical node; and a fourth partition as part of the second resources. The first and the second physical nodes are further configured to provide third resources for a plurality of storage management processes.

摘要翻译： 计算机集群包括：第一隔间，还包括多个第一物理节点，被配置为为客户虚拟实例提供第一资源，所述多个第一物理节点中的每一个还包括大容量存储设备，所述大容量存储设备还包括： 提供用于引导第一物理节点的第一分区; 和第二分区作为第一资源的一部分; 以及第二隔间，还包括被配置为为访客虚拟实例提供第二资源的多个第二物理节点，所述多个第二物理节点中的每一个还包括大容量存储设备，所述大容量存储设备包括：第三分区，其用于引导 第二个物理节点; 以及作为第二资源的一部分的第四分区。 第一和第二物理节点被进一步配置为为多个存储管理过程提供第三资源。