公开(公告)号：US20160298158A1

公开(公告)日：2016-10-13

申请号：US15191113

申请日：2016-06-23

申请人： Battelle Energy Alliance, LLC

发明人： David N. Thompson ,  William A. Apel ,  Vicki S. Thompson ,  David W. Reed ,  Jeffrey A. Lacey ,  Emily D. Henriksen

IPC分类号： C12P19/14 ,  C12N9/16 ,  C12N9/34 ,  C12N9/24 ,  C12N9/80 ,  C12N9/42 ,  C12N9/40 ,  C12N9/18 ,  C12N9/38 ,  C12P19/02 ,  C12N9/88

CPC分类号： C12P19/14 ,  C12N9/1051 ,  C12N9/16 ,  C12N9/18 ,  C12N9/2402 ,  C12N9/2408 ,  C12N9/2428 ,  C12N9/2434 ,  C12N9/2437 ,  C12N9/2445 ,  C12N9/2465 ,  C12N9/2471 ,  C12N9/248 ,  C12N9/2482 ,  C12N9/2494 ,  C12N9/80 ,  C12N9/88 ,  C12N15/52 ,  C12P19/00 ,  C12P19/02 ,  C12Y204/0102 ,  C12Y301/01001 ,  C12Y301/01006 ,  C12Y301/01073 ,  C12Y301/02004 ,  C12Y302/01008 ,  C12Y302/01015 ,  C12Y302/0102 ,  C12Y302/01021 ,  C12Y302/01022 ,  C12Y302/01037 ,  C12Y302/01055 ,  C12Y302/01078 ,  C12Y302/01091 ,  C12Y302/01131 ,  C12Y302/01133 ,  C12Y302/01139 ,  C12Y305/01 ,  C12Y402/01007 ,  C12Y402/01008

摘要： Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods of at least partially degrading, cleaving, or removing polysaccharides, lignocellulose, cellulose, hemicellulose, lignin, starch, chitin, polyhydroxybutyrate, heteroxylans, glycosides, xylan-, glucan-, galactan-, or mannan-decorating groups using isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius.

公开(公告)号：US09404134B2

公开(公告)日：2016-08-02

申请号：US14727653

申请日：2015-06-01

申请人： BATTELLE ENERGY ALLIANCE, LLC

发明人： David N Thompson ,  William A Apel ,  Vicki S Thompson ,  David W Reed ,  Jeffrey A Lacey ,  Emily D. Henriksen

IPC分类号： C12P19/14 ,  C12N9/14 ,  C12N9/16 ,  C12P21/06 ,  C12P19/34 ,  C07H21/04 ,  C07K1/00 ,  C12P19/00 ,  C12N9/10 ,  C12N9/24 ,  C12N9/26 ,  C12N9/42 ,  C12N9/40 ,  C12N9/38 ,  C12N9/88 ,  C12N15/52 ,  C12N9/18

CPC分类号： C12P19/14 ,  C12N9/1051 ,  C12N9/16 ,  C12N9/18 ,  C12N9/2402 ,  C12N9/2408 ,  C12N9/2428 ,  C12N9/2434 ,  C12N9/2437 ,  C12N9/2445 ,  C12N9/2465 ,  C12N9/2471 ,  C12N9/248 ,  C12N9/2482 ,  C12N9/2494 ,  C12N9/80 ,  C12N9/88 ,  C12N15/52 ,  C12P19/00 ,  C12P19/02 ,  C12Y204/0102 ,  C12Y301/01001 ,  C12Y301/01006 ,  C12Y301/01073 ,  C12Y301/02004 ,  C12Y302/01008 ,  C12Y302/01015 ,  C12Y302/0102 ,  C12Y302/01021 ,  C12Y302/01022 ,  C12Y302/01037 ,  C12Y302/01055 ,  C12Y302/01078 ,  C12Y302/01091 ,  C12Y302/01131 ,  C12Y302/01133 ,  C12Y302/01139 ,  C12Y305/01 ,  C12Y402/01007 ,  C12Y402/01008

摘要： Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods of at least partially degrading, cleaving, or removing polysaccharides, lignocellulose, cellulose, hemicellulose, lignin, starch, chitin, polyhydroxybutyrate, heteroxylans, glycosides, xylan-, glucan-, galactan-, or mannan-decorating groups using isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius.

摘要翻译： 提供了分离和/或纯化的多肽和编码来自酸热环酸杆菌的多肽的核酸序列。 还提供了使用分离的至少部分降解，切割或除去多糖，木质纤维素，纤维素，半纤维素，木质素，淀粉，几丁质，聚羟基丁酸酯，异木聚糖，糖苷，木聚糖，葡聚糖，半乳聚糖或甘露聚糖装饰基团的方法 和/或编码来自酸性脂多酸杆菌的多肽的纯化多肽和核酸序列。

公开(公告)号：US20240191216A1

公开(公告)日：2024-06-13

申请号：US18123840

申请日：2023-03-20

申请人： LYGOS, INC.

发明人： Jeffrey A. Dietrich ,  Jeffrey L. Fortman ,  Eric J. Steen

IPC分类号： C12N9/16 ,  C07C51/38 ,  C07C67/08 ,  C07C67/31 ,  C12P7/46

CPC分类号： C12N9/16 ,  C12P7/46 ,  C12Y301/02004 ,  C07C51/38 ,  C07C67/08 ,  C07C67/31

摘要： Systems and methods for the production of malonate in recombinant host cells.

公开(公告)号：US20180201964A1

公开(公告)日：2018-07-19

申请号：US15898378

申请日：2018-02-16

申请人： Battelle Energy Alliance, LLC

发明人： David N. Thompson ,  William A. Apel ,  Vicki S. Thompson ,  David W. Reed ,  Jeffrey A. Lacey ,  Emily D. Henriksen

IPC分类号： C12P19/14 ,  C12N9/88 ,  C12N9/80 ,  C12N9/38 ,  C12N9/40 ,  C12N9/42 ,  C12N9/34 ,  C12N9/24 ,  C12N9/26 ,  C12N9/18 ,  C12N9/16 ,  C12N9/10 ,  C12P19/02 ,  C12P19/00 ,  C12N15/52

CPC分类号： C12P19/14 ,  C12N9/1051 ,  C12N9/16 ,  C12N9/18 ,  C12N9/2402 ,  C12N9/2408 ,  C12N9/2428 ,  C12N9/2434 ,  C12N9/2437 ,  C12N9/2445 ,  C12N9/2465 ,  C12N9/2471 ,  C12N9/248 ,  C12N9/2482 ,  C12N9/2494 ,  C12N9/80 ,  C12N9/88 ,  C12N15/52 ,  C12P19/00 ,  C12P19/02 ,  C12Y204/0102 ,  C12Y301/01001 ,  C12Y301/01006 ,  C12Y301/01073 ,  C12Y301/02004 ,  C12Y302/01008 ,  C12Y302/01015 ,  C12Y302/0102 ,  C12Y302/01021 ,  C12Y302/01022 ,  C12Y302/01037 ,  C12Y302/01055 ,  C12Y302/01078 ,  C12Y302/01091 ,  C12Y302/01131 ,  C12Y302/01133 ,  C12Y302/01139 ,  C12Y305/01 ,  C12Y402/01007 ,  C12Y402/01008

摘要： Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods of at least partially degrading, cleaving, or removing polysaccharides, lignocellulose, cellulose, hemicellulose, lignin, starch, chitin, polyhydroxybutyrate, heteroxylans, glycosides, xylan-, glucan-, galactan-, or mannan-decorating groups using isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius.

公开(公告)号：US09816114B2

公开(公告)日：2017-11-14

申请号：US14386272

申请日：2013-03-06

申请人： Lygos, Inc.

发明人： Jeffrey A. Dietrich ,  Jeffrey L. Fortman ,  Eric J. Steen

IPC分类号： C12N9/26 ,  C12P7/46 ,  C12N1/20 ,  C12N15/00 ,  C12N1/14 ,  C07H21/04 ,  C12N9/16 ,  C07C67/31 ,  C07C67/08 ,  C07C51/38

CPC分类号： C12P7/46 ,  C07C51/38 ,  C07C67/08 ,  C07C67/31 ,  C12N9/16 ,  C12Y301/02004 ,  C07C69/675 ,  C07C69/38 ,  C07C57/04

摘要： Systems and methods for the production of malonate in recombinant host cells.

公开(公告)号：US20150218601A1

公开(公告)日：2015-08-06

申请号：US14624713

申请日：2015-02-18

申请人： Achim MARX ,  Markus Poetter ,  Stefan Buchholz ,  Alexander May ,  Hermann Siegert ,  Georg Fuchs ,  Birgit Alber ,  Lothar Eggeling

发明人： Achim MARX ,  Markus Poetter ,  Stefan Buchholz ,  Alexander May ,  Hermann Siegert ,  Georg Fuchs ,  Birgit Alber ,  Lothar Eggeling

IPC分类号： C12P7/42 ,  C12N9/88 ,  C08F120/10 ,  C12N9/10 ,  C08F120/06 ,  C12N9/16 ,  C12N9/02

CPC分类号： C12P7/42 ,  C08F120/06 ,  C08F120/10 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/1096 ,  C12N9/16 ,  C12N9/88 ,  C12P7/40 ,  C12P7/52 ,  C12P7/625 ,  C12Y102/04004 ,  C12Y103/99012 ,  C12Y206/01042 ,  C12Y301/02004 ,  C12Y402/01017

摘要： The present invention relates to a cell which has been modified in comparison with its wild type in such a way that it is capable of forming more, by comparison with its wild, 3-hydroxyisobutyric acid or polyhydroxyalkanoates based on 3-hydroxyisobutyric acid via methylmalonate-semialdehyde or 3-hydroxybutyryl-coenzyme A as precursors. The invention also relates to a method of generating a genetically modified cell, to the genetically modified cell obtainable by these methods, to a method of producing 3-hydroxyisobutyric acid or polyhydroxyalkanoates based on 3-hydroxyisobutyric acid, to a method of producing methacrylic acid or methacrylic esters, and to a method of producing polymethacrylic acid or polymethacrylic esters. The present invention furthermore relates to an isolated DNA, to a vector, to the use of this vector for transforming a cell, to a transformed cell, and to a polypeptide.

摘要翻译： 本发明涉及与其野生型相比已被修饰的细胞，使得其能够通过与其野生型3-羟基异丁酸或基于3-羟基异丁酸的聚羟基链烷酸通过甲基丙二酸酯 - 半醛或3-羟基丁酰辅酶A作为前体。 本发明还涉及生产基于3-羟基异丁酸的3-羟基异丁酸或聚羟基链烷酸酯的生产方法，该方法可以通过这些方法产生遗传修饰的细胞，生产甲基丙烯酸或其制备方法 甲基丙烯酸酯，以及生产聚甲基丙烯酸或聚甲基丙烯酸酯的方法。 本发明还涉及分离的DNA，载体，使用该载体转化细胞，转化细胞和多肽。

公开(公告)号：US20240141397A1

公开(公告)日：2024-05-02

申请号：US18242912

申请日：2023-09-06

申请人： Genomatica, Inc.

发明人： Robin E. Osterhout ,  Priti Pharkya

IPC分类号： C12P17/10 ,  C12N9/00 ,  C12N9/02 ,  C12N9/04 ,  C12N9/06 ,  C12N9/10 ,  C12N9/16 ,  C12N15/52 ,  C12P7/18 ,  C12P7/40 ,  C12P7/44 ,  C12P13/00 ,  C12P17/08

CPC分类号： C12P17/10 ,  C12N9/0004 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/0016 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/13 ,  C12N9/16 ,  C12N9/93 ,  C12N15/52 ,  C12P7/18 ,  C12P7/40 ,  C12P7/44 ,  C12P13/001 ,  C12P13/005 ,  C12P17/08 ,  C12Y101/01027 ,  C12Y101/0103 ,  C12Y101/01035 ,  C12Y101/01037 ,  C12Y101/01172 ,  C12Y103/01031 ,  C12Y104/01009 ,  C12Y203/01174 ,  C12Y206/01019 ,  C12Y208/03006 ,  C12Y301/02004 ,  C12Y603/03004

摘要： Provided herein are non-naturally occurring microbial organisms having biosynthetic pathways for production of target products and one or more genetic modifications that reduce a byproduct of the biosynthetic pathway. Compositions of target products from such cells and methods of using such cells are provided.

公开(公告)号：US20160208289A1

公开(公告)日：2016-07-21

申请号：US14914741

申请日：2014-08-05

申请人： Invista Technologies S.à.r.l.

发明人： Adriana Leonora BOTES ,  Alex Van Eck CONRADIE

IPC分类号： C12P5/02 ,  C12N9/10 ,  C12N9/16 ,  C12N9/88

CPC分类号： C12P5/026 ,  C12N9/13 ,  C12N9/16 ,  C12N9/88 ,  C12Y208/02 ,  C12Y301/02 ,  C12Y301/02004 ,  C12Y401/01033 ,  C12Y402/01017 ,  C12Y402/01119

摘要： The document provides methods for biosynthesizing isobutene using one or more isolated enzymes such as one or more of a hydratase such as an enzyme classified under EC 4.2.1.—and a decarboxylating thioesterase, or using recombinant host cells expressing one or more such enzymes.

摘要翻译： 该文献提供了使用一种或多种分离的酶如一种或多种水合酶如EC 4.2.1中分类的酶和脱羧硫酯酶或使用表达一种或多种这样的酶的重组宿主细胞来生物合成异丁烯的方法。

公开(公告)号：US09234218B2

公开(公告)日：2016-01-12

申请号：US12602593

申请日：2008-05-30

申请人： Achim Marx ,  Markus Poetter ,  Stefan Buchholz ,  Alexander May ,  Hermann Siegert ,  Birgit Alber ,  Georg Fuchs ,  Lothar Eggeling

发明人： Achim Marx ,  Markus Poetter ,  Stefan Buchholz ,  Alexander May ,  Hermann Siegert ,  Birgit Alber ,  Georg Fuchs ,  Lothar Eggeling

IPC分类号： C12P7/42 ,  C12P7/52 ,  C12N9/02 ,  C12P7/40 ,  C12P7/62 ,  C08F120/06 ,  C08F120/10 ,  C12N9/10 ,  C12N9/16 ,  C12N9/88

CPC分类号： C12P7/42 ,  C08F120/06 ,  C08F120/10 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/1096 ,  C12N9/16 ,  C12N9/88 ,  C12P7/40 ,  C12P7/52 ,  C12P7/625 ,  C12Y102/04004 ,  C12Y103/99012 ,  C12Y206/01042 ,  C12Y301/02004 ,  C12Y402/01017

摘要： The present invention relates to a process for the preparation of methacrylic acid or methacrylic esters, comprising the process steps of IA) preparation of 3-hydroxyisobutyric acid by a process comprising the process step of bringing a cell which has been genetically modified in comparison with its wild type in such a way that it is capable of forming more 3-hydroxyisobutyric acid, or polyhydroxyalkanoates based on 3-hydroxyisobutyric acid in comparison with its wild type, into contact with a nutrient medium comprising, as carbon source, carbohydrates, glycerol, carbon dioxide, methanol, L-valine or L-glutamate under conditions under which 3-hydroxyisobutyric acid or polyhydroxyalkanoates based on 3-hydroxyisobutyric acid are formed from the carbon source, if appropriate, isolation of the 3-hydroxyisobutyric acid from the nutrient medium and also, if appropriate, neutralization of the 3-hydroxyisobutyric acid, IB) dehydration of the 3-hydroxyisobutyric acid with formation of methacrylic acid and also, where appropriate, esterification methacrylic acid. The invention also relates to a process for the preparation of polymethacrylic acid or polymethacrylic esters.

摘要翻译： 本发明涉及一种制备甲基丙烯酸或甲基丙烯酸酯的方法，其包括以下工艺步骤：IA）通过包括以下步骤的方法制备3-羟基异丁酸，所述方法包括将经遗传修饰的细胞与其 野生型，使得其能够形成更多的3-羟基异丁酸或与其野生型相比基于3-羟基异丁酸的聚羟基链烷酸酯与营养培养基接触，所述营养培养基包含作为碳源的碳水化合物，甘油，碳 二氧化物，甲醇，L-缬氨酸或L-谷氨酸盐，其中3-羟基异丁酸或基于3-羟基异丁酸的聚羟基链烷酸酯由碳源形成的条件下，如果合适，则从营养培养基中分离出3-羟基异丁酸， 如果适当，中和3-羟基异丁酸，IB）3-羟基异丁酸脱水形成甲基丙烯酸 酸，并且在适当的情况下酯化甲基丙烯酸。 本发明还涉及一种制备聚甲基丙烯酸或聚甲基丙烯酸酯的方法。

公开(公告)号：US20150275250A1

公开(公告)日：2015-10-01

申请号：US14727653

申请日：2015-06-01

申请人： BATTELLE ENERGY ALLIANCE, LLC

发明人： DAVID N. THOMPSON ,  WILLIAM A. APEL ,  VICKI S. THOMPSON ,  DAVID W. REED ,  JEFFREY A. LACEY ,  EMILY D. HENRIKSEN

IPC分类号： C12P19/00 ,  C12N9/18

CPC分类号： C12P19/14 ,  C12N9/1051 ,  C12N9/16 ,  C12N9/18 ,  C12N9/2402 ,  C12N9/2408 ,  C12N9/2428 ,  C12N9/2434 ,  C12N9/2437 ,  C12N9/2445 ,  C12N9/2465 ,  C12N9/2471 ,  C12N9/248 ,  C12N9/2482 ,  C12N9/2494 ,  C12N9/80 ,  C12N9/88 ,  C12N15/52 ,  C12P19/00 ,  C12P19/02 ,  C12Y204/0102 ,  C12Y301/01001 ,  C12Y301/01006 ,  C12Y301/01073 ,  C12Y301/02004 ,  C12Y302/01008 ,  C12Y302/01015 ,  C12Y302/0102 ,  C12Y302/01021 ,  C12Y302/01022 ,  C12Y302/01037 ,  C12Y302/01055 ,  C12Y302/01078 ,  C12Y302/01091 ,  C12Y302/01131 ,  C12Y302/01133 ,  C12Y302/01139 ,  C12Y305/01 ,  C12Y402/01007 ,  C12Y402/01008

摘要： Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods of at least partially degrading, cleaving, or removing polysaccharides, lignocellulose, cellulose, hemicellulose, lignin, starch, chitin, polyhydroxybutyrate, heteroxylans, glycosides, xylan-, glucan-, galactan-, or mannan-decorating groups using isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius.

摘要翻译： 提供了分离和/或纯化的多肽和编码来自酸热环酸杆菌的多肽的核酸序列。 还提供了使用分离的至少部分降解，切割或除去多糖，木质纤维素，纤维素，半纤维素，木质素，淀粉，几丁质，聚羟基丁酸酯，异木聚糖，糖苷，木聚糖，葡聚糖，半乳聚糖或甘露聚糖装饰基团的方法 和/或编码来自酸性脂多酸杆菌的多肽的纯化多肽和核酸序列。