公开(公告)号：US20050271557A1

公开(公告)日：2005-12-08

申请号：US11106209

申请日：2005-04-14

申请人： Hun-joo Lee ,  Nam Huh ,  Sang-hyun Paek ,  Chang-eun Yoo

发明人： Hun-joo Lee ,  Nam Huh ,  Sang-hyun Paek ,  Chang-eun Yoo

IPC分类号： G01N33/52 ,  B01L9/00

CPC分类号： B01L9/52 ,  B01J2219/00608 ,  B01J2219/00621 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01L2300/0819

摘要： Provided is a microarray holding device. The microarray holding device includes: a solid substrate in the form of a plate in which an engaging-receiving portion for receiving a microarray substrate to be engaged therewith is formed, the engaging-receiving portion being composed of an opening; sidewalls to be aligned with sidewalls of the microarray substrate to be engaged with the engaging-receiving portion; and a base surface on which a top or bottom surface of the microarray substrate to be engaged with the engaging-receiving portion is to be placed, wherein the base surface is depressed from a top or bottom surface of the solid substrate such that the other surface of the microarray substrate is lower than the top or bottom surface of the solid substrate when the microarray substrate is engaged with the engaging-receiving portion.

摘要翻译： 提供了一种微阵列保持装置。 微阵列保持装置包括：形成板状的固体基板，其中形成用于接收与其接合的微阵列基板的接合部分，所述接合部分由开口构成; 与微阵列基板的侧壁对准以与接合部接合的侧壁; 以及要在其上放置要与所述接合部分接合的所述微阵列基板的顶表面或底表面的基面，其中所述基底表面从所述固体基底的顶表面或底表面压下，使得所述另一表面 的微阵列基板与接合部接合时，低于固体基板的顶面或底面。

公开(公告)号：US07544505B2

公开(公告)日：2009-06-09

申请号：US11295844

申请日：2005-12-06

申请人： Sung-ouk Jung ,  Nam Huh ,  Soo-suk Lee ,  Chang-eun Yoo ,  Hun-joo Lee

发明人： Sung-ouk Jung ,  Nam Huh ,  Soo-suk Lee ,  Chang-eun Yoo ,  Hun-joo Lee

IPC分类号： C12M1/34

CPC分类号： B01L3/502738 ,  B01F13/0059 ,  B01F13/0222 ,  B01L3/50273 ,  B01L7/52 ,  B01L2300/0636 ,  B01L2300/0822 ,  B01L2300/0877

摘要： Provided is an agitation device used to agitate a solution in a hybridization chamber, the agitation device including: the hybridization chamber; first and second air channels connected to ends of the hybridization chamber; a first valve disposed in the first air channel; a second valve disposed in the second air channel; an integrated air channel connecting the first and second air channels; and a pump disposed in the integrated air channel. The agitation device is suitable for effective diffusion of a sample when performing hybridization using a DNA chip. Therefore, a probe can be effectively hybridized with a target material.

摘要翻译： 提供了用于搅拌杂交室中的溶液的搅拌装置，搅拌装置包括：杂交室; 第一和第二空气通道连接到杂交室的末端; 设置在第一空气通道中的第一阀; 设置在第二空气通道中的第二阀; 连接第一和第二空气通道的集成空气通道; 以及设置在集成空气通道中的泵。 搅拌装置适用于使用DNA芯片进行杂交时样品的有效扩散。 因此，探针可以有效地与靶材料杂交。

公开(公告)号：US20060099711A1

公开(公告)日：2006-05-11

申请号：US11267005

申请日：2005-11-04

申请人： Chang-eun Yoo ,  Joon-ho Kim ,  Kyu-youn Hwang ,  Hun-joo Lee ,  Hee-kyun Lim ,  Soo-min Ma ,  Nam Huh ,  Soo-suk Lee

发明人： Chang-eun Yoo ,  Joon-ho Kim ,  Kyu-youn Hwang ,  Hun-joo Lee ,  Hee-kyun Lim ,  Soo-min Ma ,  Nam Huh ,  Soo-suk Lee

IPC分类号： G01N1/00

CPC分类号： C12Q1/6806 ,  Y10T436/25 ,  C12Q2523/308

摘要： Provided is a lysis method for cells or viruses, including: immobilizing a metal-ligand complex on a solid support; and mixing the complex immobilized on the support with a cell or virus solution. According to the lysis method, by immobilizing a chemical on a solid support to perform cell lysis, the dilution problem according to the addition of a cell lysis solution can be resolved and a separate process of removing the chemical is not required so as to reduce the steps upon LOC implementation. In addition, since a variety of solid supports, such as chips, beads, nanoparticles etc. can be used, cell lysis apparatuses of various forms can be fabricated.

摘要翻译： 提供了一种细胞或病毒的裂解方法，包括：将固定支持物上的金属 - 配体复合物固定; 并将固定在载体上的复合物与细胞或病毒溶液混合。 根据裂解方法，通过将化学物质固定在固体支持体上以进行细胞裂解，可以解决根据细胞溶解溶液的添加的稀释问题，并且不需要单独的除去化学品的方法，以减少 LOC实施步骤。 此外，由于可以使用各种固体支持体，例如芯片，珠粒，纳米粒子等，因此可以制造各种形式的细胞裂解装置。

公开(公告)号：US07732191B2

公开(公告)日：2010-06-08

申请号：US11295843

申请日：2005-12-06

申请人： Soo-suk Lee ,  Sung-ouk Jung ,  Chang-eun Yoo ,  Hun-joo Lee ,  Christopher Hansung Ko ,  Nam Huh

发明人： Soo-suk Lee ,  Sung-ouk Jung ,  Chang-eun Yoo ,  Hun-joo Lee ,  Christopher Hansung Ko ,  Nam Huh

IPC分类号： C12M1/34 ,  C12M1/36

CPC分类号： B01L7/00 ,  B01F13/0059 ,  B01F13/0222 ,  B01L3/5027 ,  B01L2200/0689 ,  B01L2300/0636 ,  B01L2300/14 ,  B01L2300/1822 ,  B01L2400/0487 ,  G01N2035/00544

摘要： Provided is a hybridization system for hybridizing a biochip including: a chamber device including at least a hybridization chamber including a support for a biochip and a first cover having a sample inlet; an agitation device including: two air channels connected to ends of the hybridization chamber; two valves disposed in the air channels; an integrated air channel to which the two air channels are connected; and an air pump disposed in the integrated air channel; and a washing and drying device including: a flow channel connected to one of the two air channels through a branched valve; a flow pump disposed in the flow channel; and a buffer inlet disposed opposite the flow channel.

摘要翻译： 提供了一种用于杂交生物芯片的杂交系统，包括：至少包括一个包括用于生物芯片的支撑物的杂交室的腔室装置和具有样品入口的第一盖子; 一种搅拌装置，包括：两个连接到杂交室端部的空气通道; 设置在空气通道中的两个阀; 两个空气通道连接的集成空气通道; 以及设置在所述集成空气通道中的空气泵; 以及洗涤干燥装置，包括：流路，其通过分支阀连接到所述两个空气通道中的一个; 设置在流路中的流量泵; 以及与流动通道相对设置的缓冲器入口。

公开(公告)号：US07648838B2

公开(公告)日：2010-01-19

申请号：US11267005

申请日：2005-11-04

申请人： Chang-eun Yoo ,  Joon-ho Kim ,  Kyu-youn Hwang ,  Hun-joo Lee ,  Hee-kyun Lim ,  Soo-min Ma ,  Nam Huh ,  Soo-suk Lee

发明人： Chang-eun Yoo ,  Joon-ho Kim ,  Kyu-youn Hwang ,  Hun-joo Lee ,  Hee-kyun Lim ,  Soo-min Ma ,  Nam Huh ,  Soo-suk Lee

IPC分类号： G01N33/48 ,  G01N33/20 ,  G01N1/00

CPC分类号： C12Q1/6806 ,  Y10T436/25 ,  C12Q2523/308

摘要： Provided is a lysis method for cells or viruses, including: immobilizing a metal-ligand complex on a solid support; and mixing the complex immobilized on the support with a cell or virus solution. According to the lysis method, by immobilizing a chemical on a solid support to perform cell lysis, the dilution problem according to the addition of a cell lysis solution can be resolved and a separate process of removing the chemical is not required so as to reduce the steps upon LOC implementation. In addition, since a variety of solid supports, such as chips, beads, nanoparticles etc. can be used, cell lysis apparatuses of various forms can be fabricated.

摘要翻译： 提供了一种细胞或病毒的裂解方法，包括：将固定支持物上的金属 - 配体复合物固定; 并将固定在载体上的复合物与细胞或病毒溶液混合。 根据裂解方法，通过将化学物质固定在固体支持体上以进行细胞裂解，可以解决根据细胞溶解溶液的添加的稀释问题，并且不需要单独的除去化学品的方法，以减少 LOC实施步骤。 此外，由于可以使用各种固体支持物，例如芯片，珠粒，纳米粒子等，因此可以制造各种形式的细胞裂解装置。

公开(公告)号：US20060154360A1

公开(公告)日：2006-07-13

申请号：US11295843

申请日：2005-12-06

申请人： Soo-suk Lee ,  Sung-ouk Jung ,  Chang-eun Yoo ,  Hun-joo Lee ,  Christopher Ko ,  Nam Huh

发明人： Soo-suk Lee ,  Sung-ouk Jung ,  Chang-eun Yoo ,  Hun-joo Lee ,  Christopher Ko ,  Nam Huh

IPC分类号： C12M1/34

CPC分类号： B01L7/00 ,  B01F13/0059 ,  B01F13/0222 ,  B01L3/5027 ,  B01L2200/0689 ,  B01L2300/0636 ,  B01L2300/14 ,  B01L2300/1822 ,  B01L2400/0487 ,  G01N2035/00544

摘要： Provided is a hybridization system for hybridizing a biochip including: a chamber device including at least a hybridization chamber including a support for a biochip and a first cover having a sample inlet; an agitation device including: two air channels connected to ends of the hybridization chamber; two valves disposed in the air channels; an integrated air channel to which the two air channels are connected; and an air pump disposed in the integrated air channel; and a washing and drying device including: a flow channel connected to one of the two air channels through a branched valve; a flow pump disposed in the flow channel; and a buffer inlet disposed opposite the flow channel.

摘要翻译： 提供了一种用于杂交生物芯片的杂交系统，包括：至少包括一个包括用于生物芯片的支撑物的杂交室的腔室装置和具有样品入口的第一盖子; 一种搅拌装置，包括：两个连接到杂交室端部的空气通道; 设置在空气通道中的两个阀; 两个空气通道连接的集成空气通道; 以及设置在所述集成空气通道中的空气泵; 以及洗涤干燥装置，包括：流路，其通过分支阀连接到所述两个空气通道中的一个; 设置在流路中的流量泵; 以及与流动通道相对设置的缓冲器入口。

公开(公告)号：US20060147960A1

公开(公告)日：2006-07-06

申请号：US11295844

申请日：2005-12-06

申请人： Sung-ouk Jung ,  Nam Huh ,  Soo-suk Lee ,  Chang-eun Yoo ,  Hun-joo Lee

发明人： Sung-ouk Jung ,  Nam Huh ,  Soo-suk Lee ,  Chang-eun Yoo ,  Hun-joo Lee

IPC分类号： C12Q1/68 ,  C12M1/34 ,  B01F13/02

CPC分类号： B01L3/502738 ,  B01F13/0059 ,  B01F13/0222 ,  B01L3/50273 ,  B01L7/52 ,  B01L2300/0636 ,  B01L2300/0822 ,  B01L2300/0877

摘要： Provided is an agitation device used to agitate a solution in a hybridization chamber, the agitation device including: the hybridization chamber; first and second air channels connected to ends of the hybridization chamber; a first valve disposed in the first air channel; a second valve disposed in the second air channel; an integrated air channel connecting the first and second air channels; and a pump disposed in the integrated air channel. The agitation device is suitable for effective diffusion of a sample when performing hybridization using a DNA chip. Therefore, a probe can be effectively hybridized with a target material.

公开(公告)号：US07819138B2

公开(公告)日：2010-10-26

申请号：US11869835

申请日：2007-10-10

申请人： Hun-joo Lee ,  Nam Huh ,  Soo-suk Lee ,  Sung-ouk Jung ,  Soo-hyung Choi

发明人： Hun-joo Lee ,  Nam Huh ,  Soo-suk Lee ,  Sung-ouk Jung ,  Soo-hyung Choi

IPC分类号： F15C1/04

CPC分类号： G05D7/0694 ,  Y10T29/494 ,  Y10T137/0753 ,  Y10T137/2191 ,  Y10T137/86187

摘要： The microfluidic device includes a rotatable platform, a plurality of connection ports disposed at a portion of the platform proximate to a shaft connection hole, the plurality of connection ports capable of being connected to an external connector for selectively injecting and discharging fluid and being closed by the connector, a trap chamber disposed at a portion of the platform further away from the shaft connection hole than the plurality of connection ports, the trap chamber including an inlet connected with at least one connection port of the plurality of connection ports, an outlet connected with another connection port of the plurality of connection ports and structures which enlarge a contact area with the fluid and a temporary storage including an inlet connected with the outlet of the trap chamber and an outlet connected with another connection port of the plurality of connection ports.

摘要翻译： 所述微流体装置包括可旋转平台，多个连接端口，设置在所述平台的靠近轴连接孔的部分处，所述多个连接端口能够连接到外部连接器，用于选择性地注入和排出流体并且由 所述连接器，设置在所述平台的远离所述轴连接孔的所述平台部分处的所述收集室，所述收集室包括与所述多个连接端口中的至少一个连接端口连接的入口，所述入口连接到所述入口 多个连接端口中的另一个连接端口和扩大与流体的接触面积的结构以及包括与捕集室的出口连接的入口的临时存储器和与多个连接端口中的另一连接端口连接的出口。

公开(公告)号：US20120090428A1

公开(公告)日：2012-04-19

申请号：US12903617

申请日：2010-10-13

申请人： Thomas C. Bowen ,  Nam Huh

发明人： Thomas C. Bowen ,  Nam Huh

IPC分类号： F16H37/06 ,  F16D21/00

CPC分类号： F16H3/08 ,  F16D21/06 ,  F16D23/12 ,  F16D2021/0646 ,  F16D2021/0692 ,  F16D2023/141 ,  F16H3/006 ,  F16H2003/0931

摘要： A dual clutch transmission with multiple gears operatively coupled thereto, comprises: first and second input shafts coaxially arranged to each other; a first clutch operatively coupled with the first input shaft to drive the transmission with a gear selected from a first set of non-consecutively numbered gears; a second clutch coaxially arranged with the first clutch and operatively coupled with the second input shaft to drive the transmission with a gear selected from a second set of non-consecutively numbered gears; first and second clutch actuating devices, each including an actuator and a pivot member, each pivot member operatively coupled between the actuator and a clutch applicator of cylindrical shape to move its clutch applicator for clutch operation; and a plurality of first and second clutch levers received in a clutch retaining housing in a circularly and alternately arranged pattern. The first and second clutch levers are configured to move the first and second clutches, respectively. The cylindrically shaped clutch applicator of the first clutch actuating device is disposed coaxially with the cylindrically shaped clutch applicator of the second clutch actuating device, and the first clutch levers and the second clutch levers can operate independently to each other upon movement of the first clutch applicator or the second clutch applicator to perform a selective clutch operation between the first and second clutches.

摘要翻译： 具有与其可操作地连接的多个齿轮的双离合器变速器包括：彼此同轴布置的第一和第二输入轴; 第一离合器，其与第一输入轴可操作地联接，以用选自第一组非连续编号的齿轮的档位来驱动变速器; 与所述第一离合器同轴地布置的第二离合器，并且与所述第二输入轴可操作地联接以利用选自第二组非连续编号的齿轮的档位驱动所述变速器; 第一和第二离合器致动装置，每个包括致动器和枢转构件，每个枢转构件可操作地联接在致动器和圆柱形形式的离合器施加器之间，以移动其离合器施加器用于离合器操作; 多个第一和第二离合器杆以圆形和交替排列的图案容纳在离合器保持壳体中。 第一和第二离合器杆分别构造成移动第一和第二离合器。 第一离合器致动装置的圆柱形离合器施加器与第二离合器致动装置的圆柱形离合器施加器同轴设置，并且当第一离合器施加器运动时第一离合器杆和第二离合器杆可以彼此独立地操作 或第二离合器施加器，以在第一和第二离合器之间执行选择性离合器操作。

公开(公告)号：US07923551B2

公开(公告)日：2011-04-12

申请号：US12110391

申请日：2008-04-28

申请人： Myo-yong Lee ,  Nam Huh ,  Joon-ho Kim

发明人： Myo-yong Lee ,  Nam Huh ,  Joon-ho Kim

IPC分类号： C07H21/00

CPC分类号： C07H21/02 ,  C12N15/1003 ,  C12N15/1006

摘要： The present invention provides a method of purifying RNA, including contacting a solid support with an acidic solution having a RNA-containing sample and a kosmotropic salt having a concentration of less than 1M, thereby binding the RNA to the solid support. According to the present invention, RNA is purified efficiently due to high RNA yield and low contamination by DNA. The present invention is particularly effective in purifying RNAs of 200 nucleotides or less.

摘要翻译： 本发明提供了一种纯化RNA的方法，包括使固体支持物与具有含RNA样品的酸性溶液和浓度小于1M的感应性盐接触，从而将RNA与固体支持物结合。 根据本发明，由于高RNA产量和低DNA污染，RNA被有效地纯化。 本发明在纯化200个或更少核苷酸的RNA中特别有效。