公开(公告)号：US11886110B2

公开(公告)日：2024-01-30

申请号：US17209814

申请日：2021-03-23

Applicant: ILLUMINA, INC.

Inventor： Timothy J. Merkel ,  Ruibo Wang ,  Daniel Wright ,  Danny Yuan Chan ,  Avishek Aiyar ,  Tanmay Ghonge ,  Neil Brahma ,  Arthur Pitera

IPC: G03F7/00 ,  B29C59/02 ,  C09D183/14 ,  G03F7/16 ,  B29K83/00 ,  B29K105/00

CPC classification number: G03F7/0002 ,  B29C59/022 ,  C09D183/14 ,  G03F7/162 ,  G03F7/168 ,  B29K2083/00 ,  B29K2105/0002

Abstract: An imprinting apparatus includes a silicon master and an anti-stick layer coating the silicon master. The silicon master includes a plurality of features positioned at an average pitch of less than about 425 nm, each of the plurality of features comprises a depression having an opening with its largest opening dimension being less than about 300 nm. The anti-stick layer includes a crosslinked silane polymer network.

公开(公告)号：US20240207846A1

公开(公告)日：2024-06-27

申请号：US18542447

申请日：2023-12-15

Applicant: ILLUMINA, INC.

Inventor： Johan Sebastian Basuki ,  Tyler J. Dill ,  Justin Fullerton ,  Tanmay Ghonge ,  Brian D. Mather ,  Wei Wei

IPC: B01L3/00

CPC classification number: B01L3/502715 ,  B01L3/502753 ,  B01L2300/0645 ,  B01L2300/0887

Abstract: An example of a flow cell includes a substrate; a plurality of reactive regions spatially separated from one another across the substrate; and a plurality of independently removable coatings respectively positioned over each of the plurality of reactive regions. Each of the plurality of reactive regions includes a polymeric hydrogel layer; and a reactive entity attached to the polymeric hydrogel layer.

公开(公告)号：US20230259034A1

公开(公告)日：2023-08-17

申请号：US18068229

申请日：2022-12-19

Applicant: ILLUMINA, INC.

Inventor： Jeffrey S. Fisher ,  Tanmay Ghonge ,  Sahngki Hong ,  Lewis J Kraft

IPC: G03F7/20

CPC classification number: G03F7/2026 ,  B29C59/026

Abstract: In a method, a resin layer of a stack (e.g., resin layer over sacrificial layer over transparent substrate) is imprinted to form a convex region including first region with first height and second region with second height

公开(公告)号：US20230285926A1

公开(公告)日：2023-09-14

申请号：US18145834

申请日：2022-12-22

Applicant: ILLUMINA, INC.

Inventor： Tanmay Ghonge ,  Brian D. Mather

IPC: B01J19/00 ,  C12Q1/6806

CPC classification number: B01J19/0046 ,  C12Q1/6806 ,  B01J2219/00722

Abstract: In an example method, a polymeric hydrogel is applied to a surface of a substrate including depressions separated by interstitial regions. Before or after the polymeric hydrogel is applied, a primer set is grafted to the polymeric hydrogel to form a grafted layer. The primer set includes cleavable first primers and uncleavable second primers. At a predetermined region of the grafted layer within a portion of at least some of the depressions, some of the cleavable first primers and some of the uncleavable second primers are altered to respectively introduce cleavable second primers and uncleavable first primers.

公开(公告)号：US20210302832A1

公开(公告)日：2021-09-30

申请号：US17209814

申请日：2021-03-23

Applicant: ILLUMINA, INC.

Inventor： Timothy J. Merkel ,  Ruibo Wang ,  Daniel Wright ,  Danny Yuan Chan ,  Avishek Aiyar ,  Tanmay Ghonge ,  Neil Brahma ,  Arthur Pitera

IPC: G03F7/00 ,  C09D183/14 ,  B29C59/02 ,  G03F7/16

Abstract: An imprinting apparatus includes a silicon master and an anti-stick layer coating the silicon master. The silicon master includes a plurality of features positioned at an average pitch of less than about 425 nm, each of the plurality of features comprises a depression having an opening with its largest opening dimension being less than about 300 nm. The anti-stick layer includes a crosslinked silane polymer network.

公开(公告)号：US20240219835A1

公开(公告)日：2024-07-04

申请号：US18530025

申请日：2023-12-05

Applicant: Illumina, Inc.

Inventor： Tanmay Ghonge ,  David Prescott ,  Daniel Wright ,  Yekaterina Rokhlenko ,  Alexandra Szemjonov ,  Francesca Patel-Burrows ,  Gavriela Moskowitz

IPC: G03F7/11 ,  B01J19/00 ,  B01L3/00 ,  G03F7/038

CPC classification number: G03F7/11 ,  B01J19/0046 ,  B01L3/502707 ,  G03F7/038 ,  B01J2219/00722 ,  B01L2200/12

Abstract: Examples of flow cells include substrates. Embodiments of the present disclosure also relate to methods of fabricating flow cell substrates. Some example workflows exploit light blocking properties of an imprint layer such that the process does not include etch steps. Such processes may be used to create substrates compatible with simultaneous paired-end sequencing methods.

公开(公告)号：US20230330617A1

公开(公告)日：2023-10-19

申请号：US18145843

申请日：2022-12-22

Applicant: ILLUMINA, INC.

Inventor： Tanmay Ghonge ,  Brian D. Mather

IPC: B01J19/00 ,  C12Q1/6806

CPC classification number: B01J19/0046 ,  C12Q1/6806 ,  B01J2219/00722

Abstract: A polymeric hydrogel is applied to a surface of a substrate including depressions separated by interstitial regions. Each depression includes a UV blocking layer positioned at a first region, and a second region that is transparent to UV and adjacent to the first region. Before or after the hydrogel is applied, a primer set is grafted to the hydrogel. The primer set includes 3′ blocked uncleavable first and second pre-primers. The hydrogel or grafted layer is removed from the interstitial regions. At a first predetermined region of the grafted layer within the second region: the first pre-primers are altered to introduce cleavable first primers; and the second pre-primers are altered to introduce uncleavable second primers. At a second predetermined region of the grafted layer within the first region: the first pre-primers are altered to introduce uncleavable first primers; and the second pre-primers are blocked to introduce cleavable second primers.