公开(公告)号：US20240226893A1

公开(公告)日：2024-07-11

申请号：US18559029

申请日：2022-05-12

Applicant: IMEC VZW ,  Janssen Biotech Inc.

Inventor： Peter Peumans ,  Olivier Henry ,  Willem Van Roy ,  Tim Stakenborg ,  Alexander Graham Irwin Tulloch

IPC: B01L3/00

CPC classification number: B01L3/502761 ,  B01L3/502715 ,  B01L2200/0652 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/161 ,  B01L2400/086 ,  B01L2400/088

Abstract: A device for sorting biological cells comprises a flow channel configured to pass a flow of liquid carrying the biological cells. The flow channel comprises at least one zone associated with a cell category. Each zone of the flow channel comprises at least one surface coated with molecules having an affinity to the cell category associated with the zone, the at least one surface of the zone being configured to modify, by the molecules of the surface, a movement of a cell belonging to the associated cell category of the zone as the cell passes the zone. The device comprises a detector configured to detect a cell exhibiting at least one modified movement in one zone of the flow channel, and to transmit a trigger signal based on the detection of the cell. The device further comprises an actuator configured to divert the detected cell based on the trigger signal.

公开(公告)号：US11220706B2

公开(公告)日：2022-01-11

申请号：US16448713

申请日：2019-06-21

Applicant: IMEC VZW

Inventor： Tim Stakenborg ,  Paolo Fiorini

IPC: C12Q1/6806 ,  B01L3/00 ,  B01L7/00 ,  C12Q1/686

Abstract: The disclosure provides methods and systems for analyzing fluid samples comprising obtaining fluid samples in at least one cavity of a substrate and introducing also buffers and/or reagents in the cavity, performing nucleic acid extraction and/or purification in the cavity, and performing nucleic acid amplification in the same cavity.

公开(公告)号：US20150155179A1

公开(公告)日：2015-06-04

申请号：US14560202

申请日：2014-12-04

Applicant: IMEC VZW

Inventor： Karolien Jans ,  Alexandra Dusa ,  Tim Stakenborg

IPC: H01L21/308 ,  H01L29/06

CPC classification number: H01L21/3085 ,  B81B2207/056 ,  B81C1/00206 ,  B82Y40/00 ,  G01N33/487 ,  H01L21/3086 ,  H01L21/32 ,  H01L21/32051 ,  H01L29/06

Abstract: A method for creating a pattern on a substrate (101) is presented, the method comprises: providing a substrate (101) comprising silicon; creating a sacrificial layer (102) on the substrate (101), wherein the sacrificial layer is formed on a first surface area (101a) of the substrate thereby leaving a second surface area (101b) exposed; depositing a first functional layer (103) at least on the second surface area (101b) of the substrate (101); removing the sacrificial layer (102); wherein: removing the sacrificial layer (102) is performed by etching the sacrificial layer (102) with an acidic aqueous solution that does not adversely affect the first functional layer (103) and the substrate (101).

Abstract translation: 提出了一种用于在衬底（101）上形成图案的方法，所述方法包括：提供包括硅的衬底（101）; 在所述基板（101）上形成牺牲层（102），其中所述牺牲层形成在所述基板的第一表面区域（101a）上，从而使第二表面区域（101b）露出; 至少在基板（101）的第二表面区域（101b）上沉积第一功能层（103）; 去除牺牲层（102）; 其中：通过用对第一功能层（103）和衬底（101）不产生不利影响的酸性水溶液蚀刻牺牲层（102）来进行去除牺牲层（102）。

公开(公告)号：US11276481B2

公开(公告)日：2022-03-15

申请号：US17040036

申请日：2019-03-22

Applicant: IMEC VZW

Inventor： Tim Stakenborg ,  Chang Chen ,  Kris Covens ,  Qing Cai ,  Maarten Fauvart

IPC: G16B50/00 ,  C12Q1/6869 ,  G06N3/12

Abstract: The present invention relates to a method for writing data comprising a sequence of bits, the data being written in a form of nucleic acid, by in-vitro enzymatically producing memory nucleic acid from a strand of memory writing substrate nucleic acid, wherein the strand of memory writing substrate nucleic acid comprises a plurality of spacer sections and memory writing sections sandwiched between the spacer sections. Each of the spacer sections comprises one or more nucleobases, and each of the memory writing sections comprises a nucleobase other than the nucleobases of an adjacent spacer section upstream of the memory writing section in a travel direction of an enzyme along the strand of memory writing substrate nucleic acid. The method comprising: repeating of: synthesising, in liquid medium comprising the strand of memory writing substrate nucleic acid contacted with the enzyme, a spacer portion of the memory nucleic acid from a spacer section by the enzyme by contacting with a solution of spacer nucleotides compatible with the nucleobases of the spacer section; halting the synthesising of the spacer portion in a position where the enzyme is reaching the memory writing section resulting from incompatibility between spacer nucleotides and nucleobases of the portion of the memory nucleic acid from the memory writing section; receiving a sub-sequence of the sequence of bits, said sub-sequence comprising at least one bit; selecting a memory nucleotide compatible with the nucleobase of the memory writing section, and comprising a first label or first modification, on a condition that said sub-sequence comprises a predetermined first sequence of bit-values, and selecting a memory nucleotide compatible with the nucleobase of the memory writing section, and comprising a second label or second modification, on a condition that said sub-sequence comprises a predetermined second sequence of bit-values; and subsequent to the halting, synthesising, in the liquid medium comprising the strand of memory writing substrate nucleic acid contacted with the enzyme, a memory portion of the memory nucleic acid from the memory writing section by the enzyme by contacting the enzyme with a solution of the selected memory nucleotide.

公开(公告)号：US11260361B2

公开(公告)日：2022-03-01

申请号：US16411811

申请日：2019-05-14

Applicant: IMEC vzw

Inventor： Philippe Vereecken ,  Brecht Put ,  Tim Stakenborg ,  Arnaud Furnemont ,  Luca Di Piazza

IPC: B01J19/00 ,  C25B3/29 ,  C25B9/19 ,  C25B9/70 ,  C01G23/047 ,  C07H21/00 ,  H01B1/12

Abstract: A device for synthesis of macromolecules is disclosed. In one aspect, the device comprises an ion-releaser having a synthesis surface comprising an array of synthesis locations arranged for synthesis of the macromolecules. The ion-releaser also includes an ion-source electrode, which is arranged to contain releasable ions and is arranged to be in contact with each of the synthesis locations of the synthesis surface, thereby release ions to the synthesis locations. The ion-releaser further comprises activating electrodes, which are arranged to be in contact with the ion-source electrode, wherein each one of the activating electrodes is arranged in association with one of the synthesis locations via the ion-source electrode. The ion-releaser is arranged to release at least a portion of the releasable ions from the ion-source electrode to one of the synthesis locations, by activation of the activating electrode associated with the synthesis location.

公开(公告)号：US20180172588A1

公开(公告)日：2018-06-21

申请号：US15736457

申请日：2016-06-30

Applicant: IMEC VZW

Inventor： Peter Peumans ,  Liesbet Lagae ,  Willem Van Roy ,  Tim Stakenborg ,  Pol Van Dorpe

IPC: G01N21/64

CPC classification number: G01N21/6408 ,  G01N21/274 ,  G01N21/6428 ,  G01N21/645 ,  G01N21/648 ,  G01N2021/6413 ,  G01N2021/6439

Abstract: Sensor devices for quantifying luminescent targets are described herein. An example device comprises a light source for exciting the targets, thus generating luminescence signals and a detector for detecting these signals, resulting in a detected signal which comprises a desired signal originating from the targets and a background signal. It moreover comprises a bleaching device for bleaching of at least part of the sources generating the background signal and a processor configured to trigger the bleaching device to start bleaching, and to trigger the light source for exciting the remaining luminescent targets which are not bleached, and to trigger the detector for detecting the luminescence signal of the remaining luminescent targets, so as to generate a measurement signal representative for the quantification of the luminescent targets.

公开(公告)号：US20180172587A1

公开(公告)日：2018-06-21

申请号：US15736414

申请日：2016-06-22

Applicant: IMEC VZW

Inventor： Peter Peumans ,  Liesbet Lagae ,  Willem Van Roy ,  Tim Stakenborg ,  Pol Van Dorpe

IPC: G01N21/64

CPC classification number: G01N21/6408 ,  G01N21/6456 ,  G01N21/648

Abstract: A sensor device for quantifying luminescent targets. The device comprises a light source for exciting the targets, thus generating luminescence signals, and a detector for detecting these signals of the targets in a cell, resulting in a detected signal comprising a desired signal and a background signal. The detector has a spatial cell resolution and/or a time resolution that is so high that only a limited number of targets will be present in the cell when measuring at low concentration and/or that only a limited number of targets add to the cell in between two measurements. A change in the number of targets in the cell can be observed in the detected signal. The device comprises a processor configured to distinguish the desired and the background signal, and to combine the detected signals of the different cells and/or moments in time, to quantify the targets.

公开(公告)号：US20150105297A1

公开(公告)日：2015-04-16

申请号：US14512762

申请日：2014-10-13

Applicant: IMEC VZW ,  Katholieke Universiteit Leuven, KU LEUVEN R&D

Inventor： Tim Stakenborg ,  Robert Neely ,  Pol Van Dorpe ,  Johan Hofkens

IPC: C12Q1/68

CPC classification number: C12Q1/68 ,  B01L3/5027 ,  B01L2300/0636 ,  B01L2300/0645 ,  C12Q1/6816 ,  G01N33/48721 ,  C12Q2527/113 ,  C12Q2563/113 ,  C12Q2563/116 ,  C12Q2565/629

Abstract: A micro-fluidic device for mapping a DNA or RNA strand labeled at a plurality of specific sites with labels suitable for generating a detection signal when interacting with a detector element, the device comprising: a micro-fluidic channel; and a plurality of detector elements for detecting the labels by acquiring the detection signals, the detector elements being positioned longitudinally along the micro-fluidic channel, each detector element having a width, successive detector elements being separated by an inter-detector gap having a width, wherein the widths of at least two of the detector elements are different and/or wherein the widths at least two of the inter-detector gaps are different.

Abstract translation: 一种微流体装置，用于将在多个特定位点处标记的DNA或RNA链与用于在与检测器元件相互作用时产生检测信号的标签进行映射，所述装置包括：微流体通道; 以及多个检测器元件，用于通过获取检测信号来检测标签，检测器元件沿着微流体通道纵向定位，每个检测器元件具有宽度，连续的检测器元件由具有宽度的检测器间间隔 ，其中所述检测器元件中的至少两个的宽度是不同的，和/或其中所述检测器间间隔中的至少两个的宽度是不同的。

公开(公告)号：US11752498B2

公开(公告)日：2023-09-12

申请号：US17475807

申请日：2021-09-15

Applicant: Imec vzw

Inventor： Benjamin Jones ,  Tim Stakenborg ,  Paolo Fiorini

IPC: B01L3/00 ,  F16K99/00 ,  B05C7/04

CPC classification number: B01L3/502707 ,  B01L3/50273 ,  B01L3/502715 ,  B01L3/502738 ,  F16K99/0021 ,  B01J2219/00353 ,  B01J2219/00389 ,  B01J2219/00414 ,  B01J2219/00418 ,  B01J2219/00637 ,  B01L3/502746 ,  B01L2200/0689 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/16 ,  B01L2400/0406 ,  B01L2400/0487 ,  B01L2400/0688 ,  B01L2400/086 ,  B05C7/04

Abstract: A fluidic device (100) is described for locally coating an inner surface of a fluidic channel. The fluidic device (100) comprises a first (101), a second (102) and a third (103) fluidic channel intersecting at a common junction (105). The first fluidic channel is connectable to a coating fluid reservoir and the third fluidic channel is connectable to a sample fluid reservoir. The fluidic device (100) further comprises a fluid control means (111) configured for creating a fluidic flow path for a coating fluid at the common junction (105) such that, when coating, a coating fluid propagates from the first (101) to the second (102) fluidic channel via the common junction (105) without propagating into the third (103) fluidic channel. A corresponding method for coating and for sensing also has been disclosed.

公开(公告)号：US10746731B2

公开(公告)日：2020-08-18

申请号：US15577311

申请日：2016-06-28

Applicant: IMEC VZW

Inventor： Rita Vos ,  Karolien Jans ,  Tim Stakenborg

IPC: G01N33/543 ,  G01N33/531

Abstract: A method for immobilizing an analyte-recognizing molecule (1) on a surface (2′) functionalized with chemical groups Y1 suitable for reacting with a chemical group X2 of a coupling molecule (7) to form a reaction product comprising a chemical group Y2 suitable for reacting with the analyte-recognizing molecule (1), the method comprising the steps of: a) Providing the functionalized surface (2′), b) Contacting the functionalized surface (2′) with a solution (6) comprising simultaneously: i) The coupling molecule (7), and ii) The analyte-recognizing molecule (1).