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公开(公告)号：US11142801B2
公开(公告)日：2021-10-12
申请号：US15766631
申请日：2016-10-07
发明人： Masami Arai , Sachio Nomura , Yuriko Nemoto , Takuya Yotani
IPC分类号： C12P19/34 , C12Q1/6886 , G01N30/88 , G01N30/02 , C12Q1/68 , C12N15/09 , C12N15/00 , C12Q1/6806 , B01J20/281
摘要： It is intended to provide a method for determining a tumor. The method for determining a tumor comprises: (1) treating genomic DNA prepared from a subject tissue or cell with bisulfite (the subject tissue or cell is derived from a patient who is affected by a tumor and is determined as (i) having MSI-H of the tumor in MSI examination and/or no or reduced expression of MLH1 in the tumor in immunohistochemical examination, and (ii) having no mutation in MLH1 in genetic examination); 2) amplifying, by PCR, DNA comprising a portion or the whole of MLH1 promoter region from the bisulfite-treated DNA; 3) subjecting the PCR amplification product to ion exchange chromatography to obtain a detection signal; 4) determining whether or not the peak of the detection signal is a peak indicating highly methylated DNA; and 5) determining the tumor as a tumor derived from a patient without Lynch syndrome when the peak is determined as a peak indicating highly methylated DNA.
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公开(公告)号：US10190172B2
公开(公告)日：2019-01-29
申请号：US15121656
申请日：2015-03-02
发明人： Yae Kanai , Eri Arai , Yuriko Nemoto , Takuya Yotani
IPC分类号： C12Q1/6827 , C12Q1/6886 , G01N30/96 , G01N30/88 , B01D15/36
摘要： It is intended to provide a rapid, convenient, and highly accurate method for determining the prognosis of cancer. The present invention provides a method for determining the prognosis of a renal cell carcinoma patient, comprising: (1) treating genomic DNA prepared from a renal tissue of a subject with bisulfite; (2) amplifying the bisulfite-treated DNA by PCR; (3) subjecting the obtained PCR amplification product to ion exchange chromatography; (4) obtaining the retention time of a detection signal obtained by the chromatography; and (5) determining the renal cell carcinoma of the subject as having poor prognosis when the result of the step (4) is shorter than a retention time serving as a reference.
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公开(公告)号：US09889447B2
公开(公告)日：2018-02-13
申请号：US14908073
申请日：2014-07-25
发明人： Yuriko Nemoto , Hiroaki Taira , Takuya Yotani , Satoru Tominaga
CPC分类号： B01L3/52 , B01L3/523 , B01L3/527 , B01L2200/025 , B01L2200/026 , B01L2200/16 , B01L2300/044 , B01L2300/0609 , B01L2300/0672 , B01L2300/0809 , B01L2300/12 , B01L2300/123 , G01N30/02 , G01N35/1002 , G01N35/1079 , G01N2030/027 , G01N2030/8822
摘要： Provided are a reagent supply method and device capable of using up a reagent in a container without a remnant to the extent possible, and supplying the reagent to an analysis device without mixing air bubbles even when the remaining amount of the reagent is extremely small. The reagent supply device includes: at least one reagent container including: a container body for storing a liquid reagent; and a reagent supply port arranged on the container body; and a support member for supporting the at least one reagent container so that the reagent supply port is positioned on a lower side of the container body. The reagent supply port is sealed by a plug including at least one nozzle penetration portion. The at least one nozzle penetration portion is formed of a material having a Shore hardness of from A5° to A90°.
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公开(公告)号：US10386277B2
公开(公告)日：2019-08-20
申请号：US14908034
申请日：2014-07-25
发明人： Hiroaki Taira , Yuriko Nemoto , Takuya Yotani , Satoru Tominaga
摘要： A mechanism for collecting a trace of a biological sample to be used for a biological sample analysis, and for preparing a diluted solution of the sample. A container for specimen preparation includes: a biological sample collecting device; and a container for sample dilution. The biological sample collecting device includes: a capillary tube having openings formed at both ends thereof; and a support member for supporting the capillary tube. The container for sample dilution includes: an opening; and a swirl flow generating member formed on an inner wall of the container for sample dilution. The capillary tube of the biological sample collecting device is arranged inside the container for sample dilution by the support member.
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公开(公告)号：US09689848B2
公开(公告)日：2017-06-27
申请号：US15026175
申请日：2014-09-30
发明人： Takuya Yotani , Yuriko Nemoto , Hideki Muraki
CPC分类号： G01N30/6047 , B01D15/20 , B65D41/0407 , B65D53/02 , G01N30/6004
摘要： Provided is a chromatography column stored in a storage container. The storage container for the column includes: a container body for storing the chromatography column; and a cover threadedly engaging with the container body. A groove for threaded engagement with the cover is formed in an inner wall of the container body. Sealing members are arranged on an inner bottom surface of the container body and a lower surface of the cover, respectively. When the container body and the cover are threadedly engaged with each other, the cover enters the container body to cause the sealing members on the inner bottom surface of the container body and the lower surface of the cover to press both a lower surface and an upper surface of the column, to thereby fix the column inside the container body and seal a fluid passage hole of the column.
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公开(公告)号：US20160223501A1
公开(公告)日：2016-08-04
申请号：US15026175
申请日：2014-09-30
发明人： Takuya Yotani , Yuriko Nemoto , Hideki Muraki
CPC分类号： G01N30/6047 , B01D15/20 , B65D41/0407 , B65D53/02 , G01N30/6004
摘要： Provided is a chromatography column stored in a storage container. The storage container for the column includes: a container body for storing the chromatography column; and a cover threadedly engaging with the container body. A groove for threaded engagement with the cover is formed in an inner wall of the container body. Sealing members are arranged on an inner bottom surface of the container body and a lower surface of the cover, respectively. When the container body and the cover are threadedly engaged with each other, the cover enters the container body to cause the sealing members on the inner bottom surface of the container body and the lower surface of the cover to press both a lower surface and an upper surface of the column, to thereby fix the column inside the container body and seal a fluid passage hole of the column.
摘要翻译： 提供存储在存储容器中的色谱柱。 该柱的储存容器包括：用于储存色谱柱的容器体; 以及与容器主体螺纹接合的盖。 在容器主体的内壁上形成有用于与盖接合的槽。 密封构件分别设置在容器主体的内底表面和盖的下表面上。 当容器主体和盖子彼此螺纹接合时，盖子进入容器主体，使密封件在容器主体的内底表面和盖的下表面上压下下表面和上部 从而将柱固定在容器主体内并密封柱的流体通道孔。
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