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公开(公告)号：US20120071357A1
公开(公告)日：2012-03-22
申请号：US13267964
申请日：2011-10-07
申请人： Jacob Kitzman , Todd Richmond , Mark D'Ascanzo , Thomas Albert , Jeffrey Jeddeloh , Christina Middle
发明人： Jacob Kitzman , Todd Richmond , Mark D'Ascanzo , Thomas Albert , Jeffrey Jeddeloh , Christina Middle
IPC分类号： C40B40/06
CPC分类号： C12Q1/6834 , C12N15/1093
摘要： The present invention provides methods and compositions for the enrichment of target nucleic acids in a microarray system. In particular, the present invention provides methods and compositions for uniform enrichment of target nucleic acid molecules in a microarray format. The present invention also provides for intentionally non-uniform enrichment among target nucleic acid molecules.
摘要翻译： 本发明提供了用于在微阵列系统中富集靶核酸的方法和组合物。 特别地，本发明提供了以微阵列形式均匀富集靶核酸分子的方法和组合物。 本发明还提供靶核酸分子之间有意的不均匀的富集。
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公开(公告)号：US08383338B2
公开(公告)日：2013-02-26
申请号：US12391001
申请日：2009-02-23
申请人： Jacob Kitzman , Todd Richmond , Mark D'Ascenzo , Thomas Albert , Matthew Rodesch , Jeffrey Jeddeloh , Christina Middle
发明人： Jacob Kitzman , Todd Richmond , Mark D'Ascenzo , Thomas Albert , Matthew Rodesch , Jeffrey Jeddeloh , Christina Middle
CPC分类号： C12Q1/6834 , C12N15/1093
摘要： The present invention provides methods and compositions for the enrichment of target nucleic acids in a microarray system. In particular, the present invention provides methods and compositions for uniform enrichment of target nucleic acid molecules in a microarray format. The present invention also provides for intentionally non-uniform enrichment among target nucleic acid molecules.
摘要翻译： 本发明提供了用于在微阵列系统中富集靶核酸的方法和组合物。 特别地，本发明提供了以微阵列形式均匀富集靶核酸分子的方法和组合物。 本发明还提供靶核酸分子之间有意的不均匀的富集。
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公开(公告)号：US20090221438A1
公开(公告)日：2009-09-03
申请号：US12391001
申请日：2009-02-23
申请人： Jacob Kitzman , Todd Richmond , Mark D'Ascenzo , Thomas Albert , Matthew Rodesch , Jeffrey Jeddeloh , Christina Middle
发明人： Jacob Kitzman , Todd Richmond , Mark D'Ascenzo , Thomas Albert , Matthew Rodesch , Jeffrey Jeddeloh , Christina Middle
CPC分类号： C12Q1/6834 , C12N15/1093
摘要： The present invention provides methods and compositions for the enrichment of target nucleic acids in a microarray system. In particular, the present invention provides methods and compositions for uniform enrichment of target nucleic acid molecules in a microarray format. The present invention also provides for intentionally non-uniform enrichment among target nucleic acid molecules.
摘要翻译： 本发明提供了用于在微阵列系统中富集靶核酸的方法和组合物。 特别地，本发明提供了以微阵列形式均匀富集靶核酸分子的方法和组合物。 本发明还提供靶核酸分子之间有意的不均匀的富集。
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公开(公告)号：US20080194414A1
公开(公告)日：2008-08-14
申请号：US11970949
申请日：2008-01-08
申请人： Thomas J. Albert , Roland Green , Todd Richmond , Michael Molla , Jeffrey Jeddeloh , Jason Patrick Affourtit , Mathreyan Srinivasan , Brian Christopher Godwin , Matthew Rodesch
发明人： Thomas J. Albert , Roland Green , Todd Richmond , Michael Molla , Jeffrey Jeddeloh , Jason Patrick Affourtit , Mathreyan Srinivasan , Brian Christopher Godwin , Matthew Rodesch
CPC分类号： C12N15/1093 , C12Q1/6834
摘要： The present invention provides novel methods for reducing the complexity of preferably a genomic sample for further analysis such as direct DNA sequencing, resequencing or SNP calling. The methods use pre-selected immobilized oligonucleotide probes to capture target nucleic acid molecules from a sample containing denatured, fragmented (genomic) nucleic acids for reducing the genetic complexity of the original population of nucleic acid molecules.
摘要翻译： 本发明提供了用于降低优选用于进一步分析的基因组样品的复杂性的新方法，例如直接DNA测序，再测序或SNP呼叫。 该方法使用预先选择的固定化寡核苷酸探针从含有变性，片段（基因组）核酸的样品中捕获靶核酸分子，以降低核酸分子的原始群体的遗传复杂性。
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公开(公告)号：US20100222232A1
公开(公告)日：2010-09-02
申请号：US12754308
申请日：2010-04-05
申请人： Thomas J. Albert , Roland Green , Todd Richmond , Michael Molla , Jeffrey Jeddeloh , Jason Patrick Affourtit , Mathreyan Srinivasan , Brian Christopher Godwin , Matthew Rodesch
发明人： Thomas J. Albert , Roland Green , Todd Richmond , Michael Molla , Jeffrey Jeddeloh , Jason Patrick Affourtit , Mathreyan Srinivasan , Brian Christopher Godwin , Matthew Rodesch
CPC分类号： C12N15/1093 , C12Q1/6834
摘要： The present invention provides novel methods for reducing the complexity of preferably a genomic sample for further analysis such as direct DNA sequencing, resequencing or SNP calling. The methods use pre-selected immobilized oligonucleotide probes to capture target nucleic acid molecules from a sample containing denatured, fragmented (genomic) nucleic acids for reducing the genetic complexity of the original population of nucleic acid molecules.
摘要翻译： 本发明提供了用于降低优选用于进一步分析的基因组样品的复杂性的新方法，例如直接DNA测序，再测序或SNP呼叫。 该方法使用预先选择的固定化寡核苷酸探针从含有变性，片段（基因组）核酸的样品中捕获靶核酸分子，以降低核酸分子的原始群体的遗传复杂性。
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公开(公告)号：US20060014164A1
公开(公告)日：2006-01-19
申请号：US11043294
申请日：2005-01-26
申请人： Michael Molla , Todd Richmond , Steven Smith , Thomas Albert
发明人： Michael Molla , Todd Richmond , Steven Smith , Thomas Albert
CPC分类号： G06F19/24 , G06F19/20 , G06F19/22 , Y10S977/792
摘要： The present invention is a method to assist in the identification of single nucleotide polymorphisms (SNP) from microarray hybridization data. Data from hybridization protocols run on microarrays often have variations in the data resulting from variations in hybridization conditions and efficiencies and variations in optical intensities. An algorithm is described to screen the results to identify those data points most likely to be real SNPs as opposed to variations in the hybridization or sensing data.
摘要翻译： 本发明是一种帮助从微阵列杂交数据鉴定单核苷酸多态性（SNP）的方法。 在微阵列上运行的杂交方案的数据通常在杂交条件和效率的变化以及光学强度的变化中导致的数据变化。 描述了一种算法来筛选结果以识别最可能是真实SNP的那些数据点，而不是杂交或感测数据的变化。
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公开(公告)号：US07904250B2
公开(公告)日：2011-03-08
申请号：US11043294
申请日：2005-01-26
申请人： Michael Molla , Todd Richmond , Steven Smith , Thomas Albert
发明人： Michael Molla , Todd Richmond , Steven Smith , Thomas Albert
IPC分类号： G01N33/50
CPC分类号： G06F19/24 , G06F19/20 , G06F19/22 , Y10S977/792
摘要： The present invention is a method to assist in the identification of single nucleotide polymorphisms (SNP) from microarray hybridization data. Data from hybridization protocols run on microarrays often have variations in the data resulting from variations in hybridization conditions and efficiencies and variations in optical intensities. An algorithm is described to screen the results to identify those data points most likely to be real SNPs as opposed to variations in the hybridization or sensing data.
摘要翻译： 本发明是一种帮助从微阵列杂交数据鉴定单核苷酸多态性（SNP）的方法。 在微阵列上运行的杂交方案的数据通常在杂交条件和效率的变化以及光学强度的变化中导致的数据变化。 描述了一种算法来筛选结果以识别最可能是真实SNP的那些数据点，而不是杂交或感测数据的变化。
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公开(公告)号：US20100331204A1
公开(公告)日：2010-12-30
申请号：US12695447
申请日：2010-01-28
申请人： Jeff Jeddeloh , Todd Richmond , Matthew Rodesch , Daniel Gerhardt , Paul Marrione , Thomas Albert
发明人： Jeff Jeddeloh , Todd Richmond , Matthew Rodesch , Daniel Gerhardt , Paul Marrione , Thomas Albert
CPC分类号： C12Q1/6806 , C12Q1/6809 , C12Q1/6837 , C12Q2565/515 , C12Q2539/101 , C12Q2563/131 , C12Q2537/149
摘要： The present invention provides methods and systems for targeted nucleic acid sequence enrichment in a sample. In particular, the present invention provides for enriching for targeted nucleic acid sequences during hybridizations in hybridization assays by first depleting non-target nucleic acid sequences.
摘要翻译： 本发明提供了样品中目标核酸序列富集的方法和系统。 特别地，本发明提供了通过首先消耗非靶核酸序列在杂交测定中的杂交期间富集靶向的核酸序列。
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公开(公告)号：US20120238477A1
公开(公告)日：2012-09-20
申请号：US13419029
申请日：2012-03-13
申请人： Tom Albert , Todd Richmond , Matthew Rodesch , Klause-Peter Stengele , Jochen Bühler , Markus Ott
发明人： Tom Albert , Todd Richmond , Matthew Rodesch , Klause-Peter Stengele , Jochen Bühler , Markus Ott
CPC分类号： C07K17/06 , B01J19/0046 , B01J2219/00432 , B01J2219/00439 , B01J2219/00596 , B01J2219/0061 , B01J2219/00626 , B01J2219/00637 , B01J2219/00659 , B01J2219/00675 , B01J2219/00711 , B01J2219/00725 , B82Y30/00 , C40B50/18
摘要： An oligopeptide microarray and methods for the synthesis thereof are presented. Further presented is a microarray on a solid support comprising at least about 10,000 oligopeptide features per cm2 and preferably at least about 50,000 oligopeptide features per cm2.
摘要翻译： 提出了寡肽微阵列及其合成方法。 还提出了一种固体支持物上的微阵列，其包含每cm 2至少约10,000个寡肽特征，优选每cm 2至少约50,000个寡肽特征。
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公开(公告)号：US20140031244A1
公开(公告)日：2014-01-30
申请号：US13767207
申请日：2013-02-14
申请人： Todd Richmond , Jason Norton , Emile F. Nuwaysir , Roland Green , Kate Nuwaysir
发明人： Todd Richmond , Jason Norton , Emile F. Nuwaysir , Roland Green , Kate Nuwaysir
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6837 , C12Q1/6811 , G06F19/20 , C12Q2537/165
摘要： The present invention provides methods for optimizing oligonucleotide hybridization probes for use in basic and clinical research. Specifically, the invention involves hybridizing serially diluted genomic sample to the oligonucleotide probes on the array, such that a signal intensity is produced for each of the probes; computationally identifying optimized probes which exhibit signal intensities that correspond to the serial dilutions of genomic sample and are reproducibly strong relative to non-optimized probes.
摘要翻译： 本发明提供用于优化用于基础和临床研究的寡核苷酸杂交探针的方法。 具体地，本发明包括将序列稀释的基因组样品与阵列上的寡核苷酸探针杂交，使得为每个探针产生信号强度; 计算识别优化的探针，其显示对应于基因组样品的系列稀释度的信号强度，并且相对于未优化的探针是可重现的。
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