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公开(公告)号：US5019034A
公开(公告)日：1991-05-28
申请号：US331263
申请日：1989-03-30
CPC分类号： A61B5/14514 , A61K41/0047 , A61N1/0412 , A61N1/325 , A61N1/327 , A61B5/14532 , A61N1/0424 , A61N1/044
摘要： An electrical process for enhancing and/or controlling transport of molecules across tissue such as human and animal skin is disclosed. The process involves the use of a high voltage, short duration electrical pulses on the tissue surface to produce electroporation. Once this effect has occurred, concentration, pressure or temperature gradients, or iontopheresis can be used to move molecules across the tissue. The process can be repeatedly applied without producing undesirable tissue damage or can be used to purposely cause highly limited tissue damage for the purpose of providing a desired, relatively long term molecular transport pathway. The occurrence of the electroporation effect can be detected by monitoring the tissue for a reversible electrical breakdown, which, along with an enhanced tissue permeability, is the characteristic effect of electroporation.
摘要翻译： 公开了用于增强和/或控制分子穿过诸如人和动物皮肤的组织的电工艺。 该过程涉及在组织表面上使用高电压，短持续时间的电脉冲以产生电穿孔。 一旦发生这种影响，可以使用浓度，压力或温度梯度，或离子透析法来移动分子穿过组织。 该方法可以重复施加而不产生不期望的组织损伤，或者可以用于故意地引起高度有限的组织损伤以提供期望的相对长期的分子转运途径。 电穿孔效应的发生可以通过监测组织的可逆电击穿来检测，其与增强的组织渗透性一起是电穿孔的特征效应。
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公开(公告)号：US5019372A
公开(公告)日：1991-05-28
申请号：US579050
申请日：1990-09-07
CPC分类号： A61K9/0024 , A61K9/0009 , A61K9/5094
摘要： A magnetically responsive composition for the modulated, sustained administration of a biologically active substance, the composition being in the form of a body sized and shaped for placement in the environment of use, the environment including an aqueous fluid, the body comprising, in admixture, a first phase comprising a biocompatible, plastically deformable, polymeric matrix, the polymeric matrix being insoluble in the environment of use, a second phase having a biologically active substance distributed throughout the matrix to be released to the aqueous fluid outside the matrix, and a third phase comprising a magnetically responsive substance encapsulated within the matrix so that, upon exposure of the body to an oscillating magnetic field, the rate of release of the biologically active substance to the aqueous fluid is increased.
摘要翻译： 一种用于调节，持续施用生物活性物质的磁响应组合物，所述组合物为身体形式，其尺寸和形状用于放置在使用环境中，所述环境包括水性流体，所述身体包含混合物， 第一阶段，其包含生物相容性，可塑性变形的聚合物基质，所述聚合物基质不溶于使用环境，第二相具有分布在整个基质中的生物活性物质以释放到基质外部的水性流体， 其包括包封在基质内的磁响应物质，使得当身体暴露于振荡磁场时，生物活性物质释放到水性流体的速率增加。
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公开(公告)号：US4443545A
公开(公告)日：1984-04-17
申请号：US337910
申请日：1982-01-08
IPC分类号： C12N9/88
CPC分类号： C12N9/88
摘要： Heparinase is produced by growing the bacterium, Flavobacterium heparinum in an improved defined medium consisting of a carbon source, two or more amino acids and several salts in the absence of protein. The carbon source concentration is specifically kept below a certain level to promote improved heparinase production. The sulfate source concentration is also specifically kept below a certain level to promote improved heparinase synthesis. Heparinase can be produced in this medium with or without the addition of an inducer compound.
摘要翻译： 通过在不存在蛋白质的情况下，在由碳源，两种或更多种氨基酸和几种盐组成的改进的限定培养基中生长细菌，肝素黄杆菌来生产肝素酶。 碳源浓度特别保持在一定水平以促进肝素酶的产生。 硫酸根源浓度也被特异地保持在一定水平以促进改善的肝素酶合成。 肝素酶可以在该培养基中产生或不加入诱导剂化合物。
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公开(公告)号：US4391797A
公开(公告)日：1983-07-05
申请号：US291769
申请日：1981-08-10
CPC分类号： A61K9/1635 , A61K9/0051 , A61K9/2027
摘要： Delivery systems manufactured in the form of polymeric compositions for the controlled delivery of macromolecules to environments of use are disclosed. The systems are characterized as two-phase compositions comprising a phase formed of an insoluble polymeric matrix having limited water sorptivity containing in admixture therein an interpenetrating phase formed of a particulate hydrophilic water swellable, biologically active macromolecular material.
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公开(公告)号：US4164560A
公开(公告)日：1979-08-14
申请号：US756892
申请日：1977-01-05
CPC分类号： A61K9/1635 , A61K9/0051 , A61K9/2027
摘要： Delivery systems manufactured in the form of polymeric compositions for the controlled delivery of macromolecules to environments of use are disclosed. The systems are characterized as two-phase compositions comprising a phase formed of an insoluble polymeric matrix having limited water sorptivity containing in admixture therein an interpenetrating phase formed of a particulate hydrophilic water swellable, biologically active macromolecular material.
摘要翻译： 公开了以聚合物组合物形式制造的用于将大分子控制递送到使用环境的输送系统。 该系统的特征在于两相组合物，其包含由不溶性聚合物基质形成的相，所述相不饱和聚合物基质具有有限的水吸收性，其中混合有由颗粒状亲水性水溶胀性生物活性大分子材料形成的互穿相。
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6.发明授权Mesh-gel constructs for cell delivery containing enzymes and/or enzyme inhibitors to control gel degradation 失效用于细胞递送的网凝胶构建体，其含有酶和/或酶抑制剂以控制凝胶降解公开(公告)号：US06699470B1
公开(公告)日：2004-03-02
申请号：US09685232
申请日：2000-10-10
IPC分类号： A01N6300
CPC分类号： A61L27/3852 , A61L27/3604 , A61L27/3843 , A61L27/52 , A61L27/58
摘要： A cell delivery device is prepared comprising a controllable degradable gel phase, meshed within a polymer substrate for use in tissue-engineering. The gel phase comprises a degradable, natural or synthetic polymer, and includes a suspension of living cells. The polymer substrate comprises a biocompatible, degradable polymer, and may be synthetic or natural. Degradation of the gel phase may be controlled by enzyme activity or adjustment of gel phase physical properties. In one embodiment, the gel phase contains an enzyme and/or enzyme inhibitor to control degradation of the gel phase. The device is useful in tissue replacement and repair, and more particularly, in the repair of cartilage tissue.
摘要翻译： 制备细胞递送装置，其包含可控制的可降解凝胶相，其在聚合物基质内啮合以用于组织工程。 凝胶相包含可降解的天然或合成聚合物，并且包括活细胞的悬浮液。 聚合物底物包含生物相容的可降解的聚合物，并且可以是合成的或天然的。 凝胶相的降解可以通过酶活性或凝胶相物理性质的调节来控制。 在一个实施方案中，凝胶相含有酶和/或酶抑制剂以控制凝胶相的降解。 该装置可用于组织更换和修复，更具体地，在软骨组织的修复中是有用的。
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公开(公告)号：US5232696A
公开(公告)日：1993-08-03
申请号：US101262
申请日：1987-09-25
CPC分类号： A61K38/465 , A61M1/3687 , A61M1/1678 , A61M2202/046
摘要： A method and apparatus for reducing the levels of low density lipoproteins (LDL) in blood is disclosed. The LDL is contacted with an enzyme which modifies it in a manner such that the LDL is rapidly removed endogenously by the patients' own metabolic processes. The enzyme may be introduced into the patient by injection, transdermal transport, nasal insufflation and ingestion. Additionally, the enzyme may be contained in a reactor for both in vivo and extracorporeal use.
摘要翻译： 公开了一种降低血液中低密度脂蛋白（LDL）水平的方法和装置。 使LDL与以其使得LDL被患者自身的代谢过程内源性快速去除的方式接触的酶接触。 可以通过注射，透皮转运，鼻吹入和摄取将酶引入患者。 此外，酶可以包含在用于体内和体外使用的反应器中。
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公开(公告)号：US5178864A
公开(公告)日：1993-01-12
申请号：US567261
申请日：1990-08-14
CPC分类号： A61K38/465
摘要： A therapeutically effective amount of soluble phospholipase A.sub.2 is administered into a subject for lowering the low density lipoproteins ("LDL") in the blood. Phospholipase A.sub.2 modifies the plasma LDL by hydrolyzing the phospholipids present in LDL. As a result, the modified LDL is rapidly removed from the bloodstream by the catabolic processes.
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公开(公告)号：US06355224B1
公开(公告)日：2002-03-12
申请号：US09156549
申请日：1998-09-17
IPC分类号： A61B5055
CPC分类号： A61K49/1857 , Y10S977/929 , Y10S977/93
摘要： The use of electroactive materials having regions of high electron density as contrast agents in magnetic resonance imaging is described. The electroactive materials may be electroactive polymers, inorganic clusters, carbon clusters, molecules that inherently exhibit electron donor-acceptor behavior having regions or moieties of high electron density, or any combination of the abovementioned contrast agents. The contrast agents of the invention decrease relaxation times of water when introduced into a subject for magnetic resonance imaging. It is particularly preferred that the contrast agents be introduced in the form of a colloidal suspension.
摘要翻译： 描述了在磁共振成像中使用具有高电子密度区域的电活性材料作为造影剂。 电活性材料可以是电活性聚合物，无机簇，碳簇，固有地表现出具有高电子密度的区域或部分的电子给体 - 受体行为的分子，或上述造影剂的任何组合。 本发明的造影剂在引入受试者的磁共振成像时减少水的松弛时间。 特别优选的是造影剂以胶态悬浮液的形式引入。
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10.发明授权Electroactive materials for stimulation of biological activity of bone marrow stromal cells 失效用于刺激骨髓基质细胞生物活性的电活性材料公开(公告)号：US06190893B1
公开(公告)日：2001-02-20
申请号：US09156317
申请日：1998-09-18
IPC分类号： C12N1300
CPC分类号： C12N13/00 , C12M35/02 , C12N5/0068 , C12N2533/30
摘要： Compositions, methods and systems are provided for the stimulation of biological activities within bone marrow stromal cells by applying electromagnetic stimulation to an electroactive material, wherein the electromagnetic stimulation is coupled to the electromagnetic material. In general the present invention involves attaching or associating the desired bone marrow stromal cells to or with a surface comprising an electroactive material, and applying electromagnetic radiation directly to the desired area. In preferred embodiments, the stimulation of biological activities within bone marrow stromal cells results from inducing one or more activities including, but not limited to, gene expression, cell growth, cell differentiation, signal transduction, membrane permeability, cell division and cell signalling. In particularly preferred embodiments, the present invention stimulates bone cell regeneration. In exemplary embodiments, the electroactive materials used in the present invention are either two-dimensional substrates such as thin films having at least one surface of an electroactive material, or in alternative embodiments, the electroactive materials are three-dimensional substrates comprising a matrix having at least one surface of an electroactive material.
摘要翻译： 提供了组合物，方法和系统，用于通过对电活性材料施加电磁刺激来刺激骨髓基质细胞内的生物活性，其中电磁刺激与电磁材料耦合。 通常，本发明涉及将期望的骨髓基质细胞附着或连接到包含电活性物质的表面或与包含电活性材料的表面相连，并将电磁辐射直接施加到所需区域。 在优选的实施方案中，刺激骨髓基质细胞内的生物活性是由于诱导一种或多种活性，包括但不限于基因表达，细胞生长，细胞分化，信号转导，膜通透性，细胞分裂和细胞信号传导。 在特别优选的实施方案中，本发明刺激骨细胞再生。 在示例性实施方案中，本发明中使用的电活性材料是二维底物，例如具有至少一种电活性材料表面的薄膜，或者在替代实施方案中，电活性材料是三维基质，其包含基质， 电活性材料的至少一个表面。
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